Objective: To study the cellular immune response and the anti-tumor effects induced by intramuscular DNA immu- nization with HER2/neu extracellular domain gene. Methods: HEH2/neu oncogene extracelltilar domain gene was isolated and inserted to pCDNA3 expression vector. The cellular immune response and its anti-tumor effects were analyzed after intramuscular DNA immunization. Results: A specific cytotoxic activity by spleen cells from HER2/neu ECD gene immunized mice was found in vitro. Tumor growth was inhibited after tumor challenge in vivo in immunized mice. Conclusion: Specific cellular immune re- sponse and anti-tumor effect were elicited by HEB2/neu extracellular domain gene immunization.

Objective: To induce an immune response by intramuscular immunization with HER2/neu oncogene extracellular do- main (ECD) DNA and observe the effect in vivo by an abortion model in mice.Methods: Human and rat HEH2/neu ECD gene were cloned and inserted into expressive vector pCDNA3. The DNA immunization, expression of HER2/neu ECD, antibody de- tection and abortion in immunized mice were studied. Results: HER2/neu ECD protein expressed on muscle cells in the injected site, specific antibody to HER2/neu was induced by DNA immunization. The average of the offspring per delivery from immu- nized mice was significantly lower than that from control group, and the abortive embryos were found in uterus from immunized mice.Conclusion: The results show that both human and rat HER2/neu ECD gene can induced effective immune response by DNA immunization.

OBJECTIVE To evaluate the medical instrument′s cleaning effect and bacterial elimination result by using multi-enzyme cleaning agent.METHODS The total 229 pieces of moderate contaminated medical instrument were randomly divided into 2 groups:control group and experiment group.The effects after routine water immersion and processing of multi-enzyme cleaning(including hand cleaning and machineone were observed and the bacteriological examination in the sample was checked.RESULTS 96% of the medical instruments in experiment group were cleaned,while only 35% of them in control group were cleaned.It was showed the cleaning effect of multi-enzyme was better than routine water immersion.In the experiment group,the contaminated rates of blood pincers and tweezers after hand cleaning with multi-enzyme were 33.7% and 25.5%,respectively,while the contaminated rates of blood pincers and tweezers after machine cleaning with multi-enzyme were 0.It indicated that the bacterial eliminate rate of machine cleaning with multi-enzyme was higher than hand cleaning with multi-enzyme.CONCLUSIONS Multi-enzyme agents are better for medical instrument cleaning.

For making a improving of cytological study doctors in the short term in the basic operated techniques and the level of diagnosis of cytology,the experience of teaching practice in the past fifteen years were reviewed.Flexible teaching ways were appearanced that such as,the reasonable teaching plan and target were maked,promotting self-learning strategies in the work,and special topic lectures and difficult cases discussion were performed with multi-media teaching,and so on.Finally, the activeness and initiative of study doctors were mobilized,and may be passably has the obtaineding in the satisfactory teaching effect.

OBJECTIVETo study and design a maternal and fetal monitoring system based on the cloud computing and internet of things, which can monitor and take smart care of the mother and fetus in 24 h.

METHODSUsing a new kind of wireless fetal monitoring detector and a mobile phone, thus the doctor can keep touch with hospital through internet. The mobile terminal was developed on the Android system, which accepted the data of fetal heart rate and uterine contraction transmitted from the wireless detector, exchange information with the server and display the monitoring data and the doctor's advice in real-time.

RESULTSThe mobile phone displayed the fetal heart rate line and uterine contraction line in real-time, recorded the fetus' grow process. It implemented the real-time communication between the doctor and the user, through wireless communication technology.

CONCLUSIONSThe system removes the constraint of traditional telephone cable for users, while the users can get remote monitoring from the medical institutions at home or in the nearest community at any time, providing health and safety guarantee for mother and fetus.

Objective To evaluate the efficacy and safety of mometasone furoate dry powder inhalation(MF DPI) in treating mild and moderate asthma .Methods The databases of PubMed ,EMBASE ,CINAHL were retrieved .The randomized ,controlled trials(RCT) on mometasone furoate dry powder inhalation in treating mild and moderate asthma were collected .The quality evalua‐tion and the data extraction were performed according to the Cochrane systematic evaluation method .The RevMan 5 .0 .2 software was adopted for conducting statistical analysis .Results A total of 9 RCT involving 1 795 patients with mild and moderate asthma were included .The meta‐analysis results showed that MF DPI 200 mcg/d improved FEV1(MD=0 .24 ,95% CI:0 .17 -0 .30) ,am‐PEF(MD= 25 .25 ,95% CI:8 .18 -42 .32) ,pmPEF(MD= 16 .00 ,95% CI:2 .10 -29 .90);MF DPI 400 mcg/d improved FEV1 (MD=0 .32 ,95% CI:0 .25-0 .39) ,amPEF(MD=36 .44 ,95% CI:23 .82-49 .05) ,pmPEF (MD=28 .50 ,95% CI:14 .11-42 .89) , which suggested that MF DPI 200 mcg/d and MF DPI 400 mcg/d improving FEV1 ,amPEF and pmPEF was higher than the place‐bo ;for reducing patient′s early morning dyspnea and albuterol dose ,MF DPI 200 mcg/d and MF DPI 400 mcg/d were superior to placebo .For reducing patient′s early morning wheezing ,MF DPI 200 mcg/d and MF DPI 400 mcg/d were not superior to placebo . For the occurrence of adverse events ,there was no statistical difference between MF DPI 200 mcg/d and MF DPI 400 mcg/d with placebo(P>0 .05) .Conclusion The existing evidence indicates that MF DPI has higher effect and safety in treating mild and mod‐erate asthma .

Objective To observe the effects of statins on ATP-binding cassette transporter 1(ABCA1) gene expression in neurons and astrocytes.Methods Primary human astrocytes and neuron cell line HCN-2 were incubated with simvastatin and pravastatin at different concentrations and under different conditions.Using RNA isolated from the cultured cells,we performed quantitative PCR to measure the ABCA1 gene expression in astrocytes and neurons.The protein expression of ABCA1 was measured by Western blot.Results The two statins significantly decreased the ABCA1 gene and protein expressions.Compared with pravastatin,simvastatin significantly reduced the expression of ABCA1 in neurons and astrocytes by 95% and 75%,respectively(both P

Object To study the callus culture of Gynura segetum (Lour.) Merr., so as to lay the foundation for the rapid propagation of its breeds.Methods The explants used in culture were root, stem and leaf of G. segerum. The MS basal medium containing different plant hormones was applied. Results The best explants were leaf of G. segetum. The medium induced callus was MS basal medium containing 1 0 mg/L NAA and 4 0 mg/L 6 BA and the callus induction rate was 64% . The sprouting rate of the callus transplanted to fitting differential medium was 86 7%.Conclusion The rapid propagation of G. segetum can be carried out by inducing callus of the plant.

Objective To study microvascular density(MVD),DNA ploidy and immunophenotype in prostatic intraepithelial neoplasia(PIN) as well as their relationship with its biological properties.Methods We studied paraffin-embedded specimens from 24 cases of benign prostatic hyperplasia(BPH),23 cases of low-grade prostatic intraepithelial neoplasia(LGPIN),and 26 cases of prostatic carcinoma(PC) with high-grade prostatic intraepithelial neoplasia(HGPIN).Image analysis was used to measure the DNA average ploidy and MVD,and after that the expressions of 34?El2,P63,P504s and CD34 were detected by immunohistochemistry SP.Results DNA ploidy and MVD in PIN were higher than those in BPH,but lower than in PC,and correlation was detected among different grades of PIN.MVD had a positive correlation with DNA aneuploidy in different grades of PIN(P

Objective To study the cell death in macrophages (THP-1) stimulated with different agonists (H37Rv-PPD or BCG-PPD) and to investigate the relationship between Toll like receptor (TLR)-2 and THP-1 apoptosis. Methods H37Rv-PPD and BCG-PPD were used to stimulate THP-1 cells for 3 h, 8 h, 15 h and 24 h, respectively with or without TLR-2 blockade. Cells were analyzed by flow cytometry to detect the TLR-2 expression. Annexin V staining and Hochest staining were performed to evaluate apoptosis. Results The apoptosis cells were increased when stimulated with BCG-PPD and the percentage was 30.2% at 24 h, which were confirmed by Hochest staining.However, the expression of TLR-2 did not increase simultaneously with percentage of 8.8% at 24 h.Nevertheless, most cells presented with necrosis form when stimulated with H37Rv-PPD and the expression of TLR-2 remained at high level with the percentage of 17.2% at 24 h, while the percent of apoptosis rate was only 7.7%. Under treatment of TLR-2 antibodies, the percentage of apoptosis decreased to 10.5% at 24 h of BCG-PPD stimulation and TLR-2 expressions were down-regulated to less than 3% at all time points; but after H37Rv-PPD stimulation, the percentage of apoptosis and TLR-2 expression did not changed obviously. Conclusions The attenuated BCG-PPD induces THP-1 apoptosis predominately, which is partially correlated with TLR-2 expression. While virulent H37Rv-PPD induces THP-1 necrosis predominately.