Objective To establish a novel method for the determination of betaine in serum by high performance liquid chromatography with UV detection.Methods Pre-column derivatization of serum was performed directly in acetonitrile without extraction with p-bromophenacyl bromide and 18-crown-6 ether as catalyst.The p-bromophenacyl ester derivatives were then separated by Supelcosil LC-SCX, using an was 0.8 ml/min and the effluent was monitored at 259 nm. Betaine was used for preparation of standard curve and quantification with external standards.Results The linearity of this method was 6.25-200.00 μmol/L,the regression was 0.999 8.The detection limit was 3.0 μmol/L.The within-day imprecision was 1.88%-3.79% ( average 3.24% ), the between-day imprecision was 3.14%-6.76% ( average 4.39% ), the recovery rates were 95.89%-102.86% (average 99.16% ).Conclusion This method is sensitive, rapid, accurate and suitable for the research and routine clinical practice.

Atrioventricular Block ; Heart Septal Defects, Ventricular ; Humans

Objective To study and discuss influencing factors of the elderly with ST elevation myocardial infarction (STEMI) underwent emergency percutaneous coronary intervention (PCI) after myocardial perfusion.Methods A total of 168 STEMI patients in our hospitalwere divided into total perfusion (CMP) group,reperfusion (PMP) group and non perfusion (NMP) group according to the integral contrast perfusion (APS) scores,including 54 cases in CMP group,96 cases in PMP group and 18 cases in NMP group.General information,coronary angiography and PCI results were compared,and the influencing factors of myocardial perfusion after primary PCI were compared by Logistic regression analysis.Results The general data showed significant difference in admission blood glucose,Killip grade,preinfarction angina incidence and balloon opening time,peak value of creatine kinase isoenzyme (CK-MB) and creatine kinase (CK) (P<0.05);In addition,there was significant difference in high thrombus load rate (P<0.05);Logistic regression analysis showed that the intravenous infusion of tirofiban,pre infarction angina,admission blood glucose,high thrombus load and onset to balloon time were factors affecting myocardial perfusion for PCI patients (P<0.05).Conclusion Blood glucose,intravenous infusion of tirofiban,preinfarction angina,high thrombus load and the onset to balloon time on admission are influencing factors for elderly STEMI patients after undergoing PCI myocardial perfusion.

Objective To study and discuss influencing factors of the elderly with ST elevation myocardial infarction (STEMI) underwent emergency percutaneous coronary intervention (PCI) after myocardial perfusion.Methods A total of 168 STEMI patients in our hospitalwere divided into total perfusion (CMP) group,reperfusion (PMP) group and non perfusion (NMP) group according to the integral contrast perfusion (APS) scores,including 54 cases in CMP group,96 cases in PMP group and 18 cases in NMP group.General information,coronary angiography and PCI results were compared,and the influencing factors of myocardial perfusion after primary PCI were compared by Logistic regression analysis.Results The general data showed significant difference in admission blood glucose,Killip grade,preinfarction angina incidence and balloon opening time,peak value of creatine kinase isoenzyme (CK-MB) and creatine kinase (CK) (P<0.05);In addition,there was significant difference in high thrombus load rate (P<0.05);Logistic regression analysis showed that the intravenous infusion of tirofiban,pre infarction angina,admission blood glucose,high thrombus load and onset to balloon time were factors affecting myocardial perfusion for PCI patients (P<0.05).Conclusion Blood glucose,intravenous infusion of tirofiban,preinfarction angina,high thrombus load and the onset to balloon time on admission are influencing factors for elderly STEMI patients after undergoing PCI myocardial perfusion.