Objective To evaluate the effects of different time courses and depths of acute normovolemic hemodilution (ANH) with hematocrit (Hct) on injury to the mucous membrane of small intestines in rabbits.Methods Thirty-two adult rabbits,weighing 2.0-2.5 kg,were randomly divided into 4 groups (n=8 each) using a random number table:control group (group C),Hct 18％ group (H1 group),Hct 15％ group (H2 group) and Hct 12％ group (H3 group).The animals were anesthetized with iv 20％ urethane 4 ml/kg,tracheostomized and mechanically ventilated.Blood samples were taken from the left femoral artery.Blood withdrawn from the femoral artery =2 ×body weight× body fluid per kilogram × (initial Hct-target Hct) ÷ (initial Hct+target Hct),and was simultaneously replaced with the equal volume of 6％ hydroxyethyl starch 130/0.4 infused over 30 min via the left femoral vein until the target Hct was achieved in H1,H2 and H3 groups.At 30 min of stabilization after puncture (T0) and 2,4 and 8 h after ANH (T1-3),blood samples were obtained from the superior mesenteric artery and vein for blood gas analysis and for determination of plasma concentrations of tumor necrosis factor-α (TNF-α) in the superior mesenteric vein.Oxygen extraction rate (ERO2) was calculated.Small intestinal mucosal tissues were obtained at T3 to observe the pathological changes with light microscope.Results Compared with those at T0,the plasma concentrations of TNF-α at T3 and ERO2 at T1-3 were significantly increased in H1 group,and the plasma concentrations of TNF-α at T3 and ERO2 at T1-3 were increased in H2 and H3 groups.The injury to the mucous membrane of small intestine was mild in group H1,and was severe in H2 and H3 groups.Conclusion ANH performed with 6％ hydroxyethyl starch causes injury to the mucous membrane of small intestine when Hct is 18％ and the time course ≥ 8 h,and when Hct ≤ 15％ and the time course ≥ 2 h in the rabbits.

Objective To investigate the effect of different degrees of acute normovolemic hemodilution (ANH) with 6% HES 200/0.5 on mucous membrane of small intestine in rabbits. Methods Thirty-two adult rabbits weighing 2.0-2.5 kg were randomly divided into 4 groups ( n = 8 each): Ⅰ control group underwent no ANH, and Ⅱ, Ⅲ, Ⅳ group underwent ANH with hematocrit (Hct) reduced to 24%, 18% and 12% respectively (group H1-3 ). The animals were anesthetized with 20% urethane 4 ml/kg, thracheostomized and mechanically ventilated (VT = 8 ml/kg, RR = 50 bpm). The body temperature was maintained at 36.5-37.6 ℃. Left carotid artery was cannulated for MAP monitoring and blood sampling. Right jugular vein was cannulated for CVP monitoring. Blood withdrawn from femoral artery was simultaneously replaced by iv infusion of equal volume of HES (200/0.5) until the target Hct was achieved. Blood samples were obtained from superior mesenteric vein (SMV)for determination of plasma concentrations of TNF-α before ANH(T0) and at 8 h after ANH (T1). Small intestinal mucosal tissues were obtained at T1 to observe the pathological changes by electron microscopy. Results Compared with those at T0, plasma concentrations of TNF-a in SMV were significantly increased at T1 in group H2 and H3 ( P ＜ 0.01 ), but no significant change was found in group H1 ( P ＞ 0.05). Compared with group C, plasma concentrations of TNF-α in SMV were significantly increased in group H2 and H3 ( P ＜ 0.01 ), but no significant change was found in group H1 ( P ＞ 0.05). No injury was found in mucous membrane of small intestine in group H1 . The injury to mucous membrane of small intestine was mild in group H2 and severe in group H3 . Conclusion ANH with 6% HES 200/0.5 does not affect mucous membrane of small intestine when Hct is reduced to 24% The injury to mucous membrane of small intestine occurs when Hct is reduced to≤ 18%.

Objective To analyze the causes of non-elective removal of pereutaneously inserted central venous catheters (PICC) in premature infants. Methods We retrospectively collected the clinical data of 104 premature infants and analyzed the causes of non-elective removal of PICC. Results PICC was removed when the treatment was finished (n = 93, elective removal group) or due to complications ( n = 7, non-elective removal group). In non-elective removal group, the percentage of catheter tip located in the subclavian jugular was signifi-cantly higher in non-elective removal group than in the elective removal group (X2 = 24.931, P = 0. 000 ). The length of inserted catheter was ( 8.4 + 2.2 ) cm in the non-elective removal group and ( ( 10.2:1:1.6) cm in the elective removal group ( t = 3. 391, P = 0. 001 ). Conclusion The main cause of non-elective removal of cathe-ters in premature infants may be that catheter tip fails to reach the vena cava.

Objective To evaluate the relationship between the heart diastolic function and pulse wave velocity in patients with diabetes mellitus.Methods Fifty-five patients with diabetes mellitus underwent echocardiogram exam,PWV,and BNP determination.Patients were divided into two groups according to the results:the group with normal heart diastolic function(n =33)and the group with abnormal heart diastolic function( n =22).Another 30 healthy volunteers served as control group.The values of PWV were compared.between the three groups.The relationship between E/E' and PWV,BNP and PWV were evaluated with correlation analysis.Results The PWV value of abnormal heart diastolic function group was significantly higher than that of other two groups.PWV was positively correlated with E/E'and BNP ( r =0.58,P ＜ 0.05 ; r =0.63,P ＜ 0.05).Conclusion PWV is associated with the heart diastolic function.PWV can serve as an important factor to predict abnormal heart diastolic function in patients with diabetes mellitus.

Heterotopic ossification is a common complication after acetabular fractures and fractures of the elbow.Heterotopic ossification often leads to severe joint movement disorder,which brings great pain to the patient.This paper reviewed the clinical research,including pathogenesis,clinical diagnosis,prevention,treatment and future directions of heterotopic ossification to investigate the effective method in prevention and treatment of heterotopic ossification.

OBJECTIVE:To optimize the extraction technology of Baiyi rectal solution. METHODS:The effects of water amount and extraction time on extraction technology were investigated by central composite design-response surface method using OD value of berberine hydrochloride and ferulic acid and the extract yield as index. Validation test was also conducted. RESULTS:The optimal extraction technology was as 11-fold water,decocting 70 min for the first time;5-fold water,decocting 30 min for the second time. In validation test,OD value of first extraction was 0.940 0,and the bias between observed and predicted values was -2.07%;that of second extraction was 0.851 8,and the bias was -2.41%. CONCLUSIONS:The extraction technology of Baiyi rectal solution is reasonable,feasible and suitable for industrial production.

This study was aimed to establish an analysis method of amentotaxus biflavone distribution in rats' tissues, including the heart, liver, spleen, lungs, kidney, brain, stomach, large intestine and small intestine, in order to investigate its distribution characteristics in tissues after rats gavaged with amentotaxus biflavone. HPLC-UV was employed to determine contents of amentotaxus biflavone in rats' tissues. The intragastric administration dose of amentotaxus biflavone was 500 mg·kg-1. Rats were sacrificed by cervical dislocation 10 min, 0.5, 1, 2, 4, 8, 12 h after intragastric administration. Tissues of the heart, liver, spleen, lungs, kidney, stomach, large intestine, small intestine and brain were removed and dissected immediately. Distribution of amentotaxus biflavone in each tissue was determined after processing. The results showed that tissue in different range had a good linear range. The lowest detection limit was 0.125 ng. The RSD of intra and inter-day was less than 10%. The absolute recovery rate of amentotaxus biflavone in each tissue was between 75.07% and 89.80%. The relative recovery rate was between 92.00% and 107.00%. Amentotaxus biflavone in each tissue was stable in different tissues in refrigerator of -20° C for 15 days. It was concluded that there were relatively large concentration differences of amentotaxus biflavone in different tissues. After intragastric administration, amentotaxus biflavone was mainly distributed in the stomach, large intestine, small intestine, liver and kidney, and then the heart, lungs and spleen. It also distributed in brain tissues through the blood-brain barrier.

Objective To select excellent tomato mutants by space treatment and to cultivate directly new varieties for production application and good seed resource.Methods Seeds of tomato cultivar were carried into space by scientific experiment recoverable satellite.The main agricultural characters of tomato mutant were observed and analyzed.Results Seeds of determinate growth habit tomato cultivar T10-3-2 were carried into space in 2002 by a recoverable satellite(Shenzhou 4).After cultivation and selection,two tomato variants(H1 and H2) and their offspring populations were obtained in SP2.Variations included quantitative character variations such as maturity,fruit characters and fruit trait,as well as genetic character variations such as changes from determinate growth to indeterminate growth of plant growth habits,and from the no shoulders to the green shoulders of fruits.These two kinds of changes were dominant mutations,and could inherit stably.The research also indicated that variations(amplitude,standard deviation and coefficient of variation) of each generation of two mutants,reduced along with generation increasing.It was showed these characters to be easily stable.Conclusion Tomato seeds loaded on spacecraft can truly form the tomato character variation,and the variation character may be stably inherited.

ObjectiveTo explore the clinical significance and characteristics about terminal sialyl residues linked to N-glycans in the tissue and bile glycoproteins from bile duct carcinoma. MethodsThe mRNA expression of sialyltransferases, including ST3Gal-Ⅲ and ST6Gal-Ⅰ, was detected in the tissue extracts of 35 cases with bile duct carcinoma(BDC) and 35 cases of benign biliary disease (BBD) by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR). With the lectin of WGA(Triticum valgaris agglutinin), which can recognise the terminal sialyl residues in N-glycans, all the specimens of BDC and BBD were subject to lectin histochemical staining, followed by computer-aided digital analysis. Lectin dot-blotting with horseradish peroxidase-labeled WGA was performed for the bile samples from each of the 35 cases of BDC and BBD. ResultsThe mRNA levels of ST3Gal-Ⅲ and ST6Gal-Ⅰ elevated in BDC(P

Objective:Explore the education content and method for the accompanying persons to enhance the capacity of taking care of patient .Methods:Life satisfaction index ( LSR-A) Scale and accompanying persons chaperone capability questionnaire are the tools of this research .This research trained the 45 accompanying persons care ability, and used before and after comparison .Results:Before the intervention , Most of the persons′life satis-faction levels was at a low level (41 persons′≤13), only 4 (8.9%) at a medium level.After the intervention, life satisfaction levels was improved (27 persons′≥14).Life sat!sfaction was improved (P<0.05).Chaperone capability situation was improved , for the P values in all the examination were <0 .05 .Conclusion:The manage-ment could strengthen the support for accompanying persons , could effectively improve their life satisfaction and im-prove their ability to accompany , thus improving the quality of patient care .The hospital should also consider the nursing ethical problems in accompanying persons education .