1.Application of polysaccharides from marine organism in enternal nutrition
Bingna CAI ; Huili SUN ; Yuantao WU ; Peng WAN
Parenteral & Enteral Nutrition 2010;17(2):109-112,117
With the rapid development of molecular nutrition, the urgent demand of rationalizing and individual clinical enternal nutrition support was realized. The enteral nutrition was added with special nutrition substrate. The application of carbohydrate in enteral nutrition and the status of comprehensive use of polysaccharides were summarized in this article. Based on the study of traditional fiber in enteral nutrition(EN) , the application of marine polysaccharides on the diseases oriented EN was analyzed and discussed. So, new nutrition substrate from marine polysaccharides is proposed for exploitation of various diseases oriented EN. Therefore, the individual nutrition support becomes possible.
2.An animal experimental study on access to mediastinum with flexible endoscope via suprasternal fossa
Yongchao ZHANG ; Enqiang LINGHU ; Daqing JIN ; Weimin LI ; Xiaole PENG ; Xiulan ZHANG ; Huili GUO
Chinese Journal of Digestive Endoscopy 2014;31(5):269-271
Objective To evaluate the feasibility and safety of mediastinum examination with flexible endoscope via suprasternal fossa.Methods A total of 20 healthy pigs were enrolled.Mediastinocosopy was performed via a tunnel to thoracix through a 1.5 cm incision on suprastemal fossa.Mediastinum was observed and complications were recorded.Results Mediastinocosopy was successfully achieved in all pigs.No severe bleeding or death occurred during the procedure.Five pigs got fever after the procedure with elevated white blood cell count.Four pigs had pneumothorax and three of them died in 1-6 days.Conclusion Mediastinocosopy with flexible endoscope through suprastemal fossa is feasible to diagnose mediastinal diseases,which can be important experimental evidence for the mini-invasive therapy of mediastimum diseases.
3.Evaluation of the effect of non-intubation anesthesia with ketamine combined with dexmedetomidine for elective surgery after burns in children
Chinese Journal of Postgraduates of Medicine 2021;44(12):1096-1099
Objective:To investigate the clinical effect of non-intubation anesthesia with ketamine combined with dexmedetomidine for elective surgery after burns in children.Methods:From January 2018 to December 2019, 50 children with burns who underwent elective surgery and were admitted to the Hanchuan People′s Hospital were enrolled and they were divided into the control group and the observation group by random number table, with 25 cases in each group. Both groups were given ketamine 1-2 mg/kg for induction of anesthesia, the control group was given propofol 4 mg/(kg·h) constant-rate pump injection, and the observation group was given dexmedetomidine 1 μg/(kg·h) pump injection for 10 min and then 0.5 μg/(kg·h) to maintain pump injection. The number of basic anesthesia, operation time and intraoperative fluid infusion between the two groups were compared. The mean arterial pressure (MAP), heart rate (HR) and blood oxygen saturation (SpO 2) at enter the operating room (T 1), 1 min before operation (T 2), 3 min after operation (T 3), end of the operation (T 4), recovery (T 5) points in the two groups were recorded and compared. The occurrence of adverse anesthesia reactions in the two groups and postoperative recovery were compared. Results:There was no statistically significant difference in the number of basic anesthesia, operation time, and intraoperative fluid infusion between the two groups ( P>0.05). The levels of MAP and HR did not change significantly at different time points in the control group ( P>0.05); the level of SpO 2 at T 2 and T 3 was lower than that at T 1 and was lower than that in the observation group at the same time point ( P<0.05); the levels of MAP and HR in the observation group at T 2, T 3, T 4, and T 5 were significantly lower than that at T 1, and were lower than that in the control group at the same time point ( P<0.05). The incidence of adverse reactions in the control group was higher than that in the observation group: 24.0%(6/25) vs. 4.0%(1/25), and the difference was statistically significant ( P<0.05); the scores of sedation and restlessness scale in the control group was higher than that in the observation group: (3.14 ± 0.76) scores vs. (1.22 ± 0.41) scores, the scores of the Ramsay score in the control group was lower than that in the observation group: (1.53 ± 0.36) s cores vs.(3.27 ± 30.41) scores, and the differences were statistically significant ( P<0.05). Conclusions:It is safe and effective to use ketamine combined with dexmedetomidine for non-intubation anesthesia during elective surgery after burns in children. The clinical anesthesia effect is significantly better than that of ketamine combined with propofol.
4. Arthroscopic bridging reconstruction of irreparable massive rotator cuff tears using autogenous fascia lata
Wei DING ; Minzhe ZHENG ; Mingguang BI ; Ting ZHANG ; Lingxiao PAN ; Zhaoxiang PENG ; Peixing HU ; Jin LI ; Shaohua DING
Chinese Journal of Orthopaedics 2020;40(3):138-145
Objective:
To investigate the clinical outcomes of arthroscopic bridging reconstruction of irreparable massive rotator cuff tears using autogenous fascia lata.
Methods:
From July 2015 to July 2017, a total of 10 cases (4 male and 6 female) who were treated with arthroscopic bridging reconstruction for irreparable massive rotator cuff tears using autogenous fascia lata were retrospectively analyzed. The age before surgery was 61.3±2.9 years (range 57-67 years). There were 7 patients with right shoulders and 3 with left shoulders. The dominate sides were involved in 7 cases. The trauma history was documented in 2 shoulders. The duration of preoperative symptoms was 14.0±13.5 months (1-48 months). The case with revision surgery was not included. The patients were examined with magnetic resonance imaging (MRI) to evaluate the healing of fascia lata patch bridging in the joint at one week, six months, one year and two years after operation. The motion range of shoulder and the clinical scores, including visual analogue scale (VAS), University of California Los Angeles (UCLA) score, Constant-Murley score and American Shoulder & Elbow Surgeons (ASES) score, were measured before surgery and at follow-up duration.
Results:
All cases were reconstructed the horizontal couple. No perioperative complication was occurred and all surgery were completed safely and successfully. At the end of two years, the score of ASES was 92.2±3.5 (range 88.3-98.3), UCLA 31.6±2.0 (range 28-34), Constant-Murley 85.2±5.4 (range 78-93) with significant difference (
5. A multicenter, retrospective study of pathogenic bacteria distribution and drug resistance in febrile neutropenic patients with hematological diseases in Shanghai
Jun ZHU ; Jiong HU ; Yuanfei MAO ; Fangyuan CHEN ; Jianyi ZHU ; Jumei SHI ; Dandan YU ; Siguo HAO ; Rong TAO ; Peng LIU ; Shiyang GU ; Jian HOU ; Haiyan HE ; Aibin LIANG ; Yi DING ; Ligen LIU ; Yinghua XIE ; Qi ZHU ; Yehua YU ; Yonghua YAO ; Wei CHEN ; Huili XU ; Xiuhua HAN ; Chun WANG
Chinese Journal of Hematology 2017;38(11):945-950
Objective:
To investigate the pathogen spectrum distribution and drug resistance of febrile neutropenic patients with hematological diseases in Shanghai.
Methods:
A retrospective study was conducted on the clinical isolates from the febrile neutropenic patients hospitalized in the departments of hematology in 12 general hospitals in Shanghai from January 2012 to December 2014. The drug susceptibility test was carried out by Kirby-Bauer method. WHONET 5.6 software was used to analyze pathogenic bacteria and drug susceptibility data.
Results:
A total of 1 260 clinical isolates were collected from the febrile neutropenic patients. Gram-positive bacteria accounted for 33.3% and Gram-negative bacteria accounted for 66.7%.
6.Periostin inhibits hypoxia-induced oxidative stress and apoptosis in human periodontal ligament fibroblasts p38 MAPK signaling pathway.
Huili LIU ; Yidan WANG ; Yangli YUE ; Peng ZHANG ; Yali SUN ; Qiaohua CHEN
Journal of Zhejiang University. Medical sciences 2020;40(7):942-948
OBJECTIVE:
To investigate the effect of periostin on hypoxia-induced oxidative stress and apoptosis in human periodontal ligament fibroblasts and the molecular mechanism involved.
METHODS:
cultured human periodontal ligament fibroblasts were placed in an anaerobic gas-producing bag for hypoxia treatment for 48 h followed by treatment with periostin at low (25 ng/mL), moderate (50 ng/mL) or high (100 ng/mL) doses. MTT assay was used to measure the cell viability, and the cell apoptosis rate was determined using flow cytometry. The contents of IL-1β, IL-6 and TNF-α in the cells were determined with ELISA, and ROS levels were measured using a fluorescent plate reader. The intracellular SOD activity was detected using ELISA. The expressions of HIF-1α, P21, cyclin D1, Bax, cleaved caspase-3, Bcl-2, P38MAPK and p-p38 MAPK proteins in the cells were detected with Western blotting.
RESULTS:
Hypoxia treatment significantly reduced the cell viability ( < 0.05), increased P21, Bax, and cleaved caspase-3 protein levels ( < 0.05), promoted cell apoptosis ( < 0.05), and decreased cyclin D1 and Bcl-2 protein levels ( < 0.05) in the cells. Compared with the hypoxic group, the cells treated with periostin at different concentrations showed significantly increased cell viability ( < 0.05) with significantly lowered apoptotic rates ( < 0.05) and decreased expression levels of Bax and cleaved caspase-3 ( < 0.05) but significantly increased expression levels of cyclin D1 and Bcl-2 ( < 0.05). Hypoxic exposure of the cells resulted in significantly increased expression levels of HIF-1α and p-p38 MAPK ( < 0.05) and increased levels of IL-1β, IL-6, TNF-α and ROS ( < 0.05) but decreased SOD activity ( < 0.05). Periostin treatment at different concentrations significantly lowered the expression levels of HIF-1α and p-p38 MAPK ( < 0.05) and the levels of IL-1β, IL-6, TNF-α and ROS ( < 0.05) and significantly increased SOD activity in the hypoxic cells ( < 0.05).
CONCLUSIONS
Periostin promotes the proliferation, inhibits apoptosis, enhances cellular antioxidant capacity, and reduces inflammatory damage in human periodontal ligament fibroblasts exposed to hypoxia possibly by inhibiting the activation of the p38 MAPK signaling pathway.
Apoptosis
;
drug effects
;
Cell Adhesion Molecules
;
administration & dosage
;
Cell Hypoxia
;
Fibroblasts
;
drug effects
;
Humans
;
Oxidative Stress
;
drug effects
;
Periodontal Ligament
;
cytology
;
Signal Transduction
;
drug effects
;
p38 Mitogen-Activated Protein Kinases
7.Periostin inhibits hypoxia-induced oxidative stress and apoptosis in human periodontal ligament fibroblasts p38 MAPK signaling pathway.
Huili LIU ; Yidan WANG ; Yangli YUE ; Peng ZHANG ; Yali SUN ; Qiaohua CHEN
Journal of Southern Medical University 2020;40(7):942-948
OBJECTIVE:
To investigate the effect of periostin on hypoxia-induced oxidative stress and apoptosis in human periodontal ligament fibroblasts and the molecular mechanism involved.
METHODS:
cultured human periodontal ligament fibroblasts were placed in an anaerobic gas-producing bag for hypoxia treatment for 48 h followed by treatment with periostin at low (25 ng/mL), moderate (50 ng/mL) or high (100 ng/mL) doses. MTT assay was used to measure the cell viability, and the cell apoptosis rate was determined using flow cytometry. The contents of IL-1β, IL-6 and TNF-α in the cells were determined with ELISA, and ROS levels were measured using a fluorescent plate reader. The intracellular SOD activity was detected using ELISA. The expressions of HIF-1α, P21, cyclin D1, Bax, cleaved caspase-3, Bcl-2, P38MAPK and p-p38 MAPK proteins in the cells were detected with Western blotting.
RESULTS:
Hypoxia treatment significantly reduced the cell viability ( < 0.05), increased P21, Bax, and cleaved caspase-3 protein levels ( < 0.05), promoted cell apoptosis ( < 0.05), and decreased cyclin D1 and Bcl-2 protein levels ( < 0.05) in the cells. Compared with the hypoxic group, the cells treated with periostin at different concentrations showed significantly increased cell viability ( < 0.05) with significantly lowered apoptotic rates ( < 0.05) and decreased expression levels of Bax and cleaved caspase-3 ( < 0.05) but significantly increased expression levels of cyclin D1 and Bcl-2 ( < 0.05). Hypoxic exposure of the cells resulted in significantly increased expression levels of HIF-1α and p-p38 MAPK ( < 0.05) and increased levels of IL-1β, IL-6, TNF-α and ROS ( < 0.05) but decreased SOD activity ( < 0.05). Periostin treatment at different concentrations significantly lowered the expression levels of HIF-1α and p-p38 MAPK ( < 0.05) and the levels of IL-1β, IL-6, TNF-α and ROS ( < 0.05) and significantly increased SOD activity in the hypoxic cells ( < 0.05).
CONCLUSIONS
Periostin promotes the proliferation, inhibits apoptosis, enhances cellular antioxidant capacity, and reduces inflammatory damage in human periodontal ligament fibroblasts exposed to hypoxia possibly by inhibiting the activation of the p38 MAPK signaling pathway.
Apoptosis
;
Fibroblasts
;
Humans
;
Hypoxia
;
Oxidative Stress
;
Periodontal Ligament
;
Signal Transduction
;
p38 Mitogen-Activated Protein Kinases
8.Extraction Technology of Volatile Oil from Wenjing Huoxue Cataplasm
Huili PENG ; Lina ZHAO ; Yanqin CHENG ; Liyan ZHAO ; Yinglu FENG ; Mingchun LI
Journal of Pharmaceutical Practice 2023;41(8):492-495
Objective To optimize the supercritical CO2 extraction conditions of volatile oil from Wenjing Huoxue cataplasm. Methods On the basis of single factor investigation on the comprehensive score of extraction yield , osthole content and isoimperatorin, the effects of extraction temperature, pressure and time on the comprehensive score of extracted volatile oil were optimized by orthogonal design. Results In the single factor experiment, the factors that had a great influence on the comprehensive score of the extracted volatile oil were extraction temperature, extraction pressure and extraction time. The orthogonal experiment results showed that the extraction temperature and extraction pressure had a significant influence on the comprehensive score of volatile oil. The optimized extraction process was as follows: extraction temperature at 55 ℃, extraction pressure as 30 MPa, and extraction time as 2 h. Conclusion The extraction process optimized in this experiment is stable and feasible, which could be used for the extraction and preparation of the volatile oil.
9.Outcomes of neoadjuvant chemoradiotherapy followed by total mesorectal excision surgery for locally advanced rectal cancer: a single-institution experience.
Michelle Shu Fen TSENG ; Huili ZHENG ; Ivy Wei Shan NG ; Yiat Horng LEONG ; Cheng Nang LEONG ; Wei Peng YONG ; Wai Kit CHEONG ; Jeremy Chee Seong TEY
Singapore medical journal 2018;59(6):305-310
INTRODUCTIONNeoadjuvant chemoradiotherapy (CRT) followed by total mesorectal excision (TME) surgery for locally advanced rectal cancer has been shown to improve local control and reduce toxicity, as compared to adjuvant CRT. We reported the outcomes of our patients with locally advanced rectal cancer treated at National University Hospital, Singapore.
METHODSFrom April 2002 to December 2014, 117 patients with T3/4, N0/+, M0 rectal cancer received neoadjuvant CRT followed by TME surgery. The treatment regimen comprised a total radiotherapy dose of 50.4 Gy in 28 daily fractions delivered concurrently with 5-fluorouracil or capecitabine chemotherapy over 5.5 weeks. All patients were planned for TME surgery. Local control, disease-free survival, overall survival and treatment toxicities were analysed.
RESULTSMedian follow-up was 34 (range 2-122) months. 11.5% (13/113) of patients achieved a pathological complete response (pCR) and 72.6% (85/117) had either tumour or nodal downstaging following neoadjuvant CRT. 5.2% (5/96) of patients had Grade 3 acute toxicities (dermatitis and diarrhoea) and 3.1% (3/96) had Grade 3 late toxicities (fistula and stricture). There was no Grade 4 toxicity noted. The five-year local recurrence, disease-free survival and overall survival rates were 4.5%, 65.7% and 80.6%, respectively. Multivariate analysis showed that nodal positivity was a predictor of poor disease-free survival and poor overall survival. Tumour downstaging and pCR did not improve outcomes.
CONCLUSIONOur outcomes were comparable to internationally published data, and this treatment regimen remains the standard of care for locally advanced rectal cancer in our local population.
10.Primary cilia mediate Klf2-dependant Notch activation in regenerating heart.
Xueyu LI ; Qiang LU ; Yuanyuan PENG ; Fang GENG ; Xuelian SHAO ; Huili ZHOU ; Ying CAO ; Ruilin ZHANG
Protein & Cell 2020;11(6):433-445
Unlike adult mammalian heart, zebrafish heart has a remarkable capacity to regenerate after injury. Previous study has shown Notch signaling activation in the endocardium is essential for regeneration of the myocardium and this activation is mediated by hemodynamic alteration after injury, however, the molecular mechanism has not been fully explored. In this study we demonstrated that blood flow change could be perceived and transmitted in a primary cilia dependent manner to control the hemodynamic responsive klf2 gene expression and subsequent activation of Notch signaling in the endocardium. First we showed that both homologues of human gene KLF2 in zebrafish, klf2a and klf2b, could respond to hemodynamic alteration and both were required for Notch signaling activation and heart regeneration. Further experiments indicated that the upregulation of klf2 gene expression was mediated by endocardial primary cilia. Overall, our findings reveal a novel aspect of mechanical shear stress signal in activating Notch pathway and regulating cardiac regeneration.