Objective To investigate the effects of different doses of gabapentin on streptozotocin (STZ)-induced diabetic neuropathic pain in rats.Methods Male SD rats aged 6 weeks weighing 180-200 g were used in this study. Diabetes ntellitus ( DM) was induced by intraperitoneal STZ 60 mg/kg and confirmed one week later by blood glucose =16.7 mmol/L before breakfast. The DM rats were randomly divided into 4 groups ( n = 6 each) : gabapentin groups received intraperitoneal gabapentin 30, 60 and 120 mg/kg twice a day (at 9:00 am and 3:00 pm) for 3 weeks respectively and control group received intraperitoneal normal saline 0.6 ml instead of gabapentin. The paw withdrawal threshold to von Frey filament stimulation was measured before and at 30, 60, 120, 180, 240 min after first gabapentin injection and once a week for 3 weeks. Results After gabapentin 60 and 120 mg/kg, the paw withdrawal threshold to mechanical stimuli was significantly increased and lasted for about 4 h. The analgesic effect peaked at 60 min after IP gabapentin injection. Normal saline and gabapentin 30 mg/kg had no significant analgesic effect. The degree of analgesia was significantly decreased at day 14 and 21 of treatment with gabapentin 60 and 120 mg/kg as compared with that at 60 min after gabapentin injection. Conclusion The hyperalgesia and allodynia in rats with diabetes mellitus can be effectively reversed by gabapentin 60 and 120 mg/kg,while long-term use of gabapentin can induce drug tolerance.

Objective:To develop an HPLC wavelength switching method for the determination of seven components in Shenrong Guben tablets simultaneously.Methods:A Waters Sunfire C18column (250 mm ×4.6 mm,5 μm) was adopted. The column temperature was set at 30 ℃. The mobile phase was acetonitrile-0.1% phosphate solution with gradient elution. The flow rate was 0.9 ml·min-1. The detection wavelength were set at 330 nm for verbascoside and martinoside,230 nm for albiflorin and paeoniflorin,and 203 nm for ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1. Results:The linear range of verbascoside, martinoside,albiflorin, paeoniflorin, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1was 6.38-159.50 μg·ml-1(r =0.999 3),3.19-79.75 μg·ml-1(r =0.999 9),4.37-109.25 μg·ml-1(r =0.999 5),14.26-356.50 μg·ml-1(r =0.999 4), 1.95-48.75 μg·ml-1(r = 0.999 8), 2.21- 55.25 μg·ml-1(r = 0.999 7) and 2.09- 52.25 μg·ml-1(r = 0.999 1), respectively. The average recovery and the corresponding RSD was 98.24% (1.11%),97.64% (1.43%),99.23% (0.80%), 100.13% (0.65%),96.99% (1.56%),98.10% (1.24%) and 97.75%(1.37%),respectively. Conclusion:The developed method can determine the contents of verbascoside, martinoside, albiflorin, paeoniflorin, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1in Shenrong Guben tablets simultaneously,which can be applied in the quality control of Shenrong Guben tablets.

ObjectiveTo obtain the status of latent tuberculosis infection （LTBI） among tuberculosis （TB）-related health-care workers （HCWs） in Shanghai， and to explore the risk factors related to TB infection. MethodsA multi-center cross-sectional study was conducted by recruiting medical workers from multiple designated TB hospitals， centers for disease control and prevention， and community health service centers in Shanghai. Each subject was required to complete a questionnaire and to provide a blood sample for TB infection test. Univariate and multivariate analysis ware made in order to find risk factors relating to TB infection. ResultsA total of 165 medical workers were recruited， and the proportion of TB infection was 16.36% （95%CI： 11.49%‒22.76%）. Multivariate logistic analysis showed that clinical doctors and nurses （adjusted OR=9.756， 95%CI： 1.790‒53.188）， laboratory staffs （adjusted OR=78.975， 95%CI： 8.749‒712.918）， and nursing and cleaning workers （adjusted OR=89.920， 95%CI： 3.111‒2 598.930） had higher risk of TB infection. ConclusionThe overall LTBI prevalence among TB-related HCWs is low. However， working as doctors， nurses， laboratory staffs， nursing workers and cleaning workers are risk factors of TB infection. TB-related HCWs who work at hospitals are at risk of TB infection comparing to medical staffs who work outside hospitals.