Objective To evaluate the expression of annexin V in maternal blood and placenta,and to explore the relationship between annexin V and preeclampsia(PE). Methods 120 women with PE who delivered babies in the Second Hospital of Hebei Medical University from December 2007 to December 2009 were chosen as study groups. They were classified into four groups: early-onset mild group (n =30),early-onset severe group (n=30), late-onset mild group ( n = 30) and late-onset severe group (n=30). 30women without perinatal complications who accepted elective term cesarean section were chosen as control group. Western blot and immunohistochemistry were used to detect the expression and localization of annexin V in placenta and maternal blood. Flow cytometry was employed to detect the apoptosis of cytotrophoblast.Annexin V mRNA level was determined by reverse transcription (RT)PCR. Prothrombin time (PT),activated partial thromboplastin time (APTT), fibrinogen (FiB), international normalized ratio (INR) were detected in each group. Results (1) The expression of annexin V in placenta and maternal blood were:0.54±0.12 and 0.62±0.17 in early-onset mild group; 0.47±0.15 and 0.56±0.24 in early-onset severe group; 0.74±0.23 and 1.08±0.32 in late-onset mild group; 0.68±0.28 and 0.72±0.21 in late-onset severe group;1.73±0.35 and 1.55±0.27 in control group. They were significantly lower in PE groups than in control group (P＜0.05 ). However, there was no significant difference among PE groups (P＞0. 05). (2) The early apoptosis, late apoptosis percentage of trophoblast cells were: 3. 21%, 0. 86%, in early-onset mild group; 5.32%, 0. 72%, in early-onset severe group; 2. 43%, 0. 63%, in late-onset mild group;4. 28%, 0. 48% in late-onset severe group; 1.05%, 0. 59%, in control group. Early apoptosis percentage in each group of PE was higher than that in control group ( P ＜ 0. 05 ). However, there was no significant difference among PE groups (P＞0.05). (3) The annexin V mRNA levels in placenta were:25.0±3.0 in early-onset mild group; 24. 8 ± 3.0 in early-onset severe group; 25.4 ± 3. 9 in late-onset mildgroup; 25.1±2.7 in late-onset severe group, respectively. All were significantly higher than that in control group (30. 6±3.0, P＜ 0. 05), and no significant difference was found among PE groups (P＞0.05).(4) PT, APTT, FiB, INR levels were: (11.3±2.4), (25.6±2.9) s, (4.6±0.9) g/L and 0.9 ±0.2in early-onset mild group; (12.1±1.9), (27. 2 ±2. 1 ) s, (5.0 ± 1. 0) g/L and 0. 9 ±0. 2 in early-onset severe group; (11.7±2.3), (26.5±2.3)s,(5.0±0.7)g/L and 0.8±0.3 in late-onset mild group;(11.4±2.6), (27.3±3.0) s, (4.3 ±0.8) g/L and 0.8 ±0.3 in late-onset severe group; (12.4±2.7), ( 28.0±1.9) s, (5.1±1.2) g/L and 0.9 ± 0.2 in control group. There was no significant difference among PE groups and control group ( P ＞ 0.05 ). Conclusion The expression changes of annexin V in placenta and maternal blood were observed in patients with PE. This indicated that annexin V played an important role in the pathogenesis and progression of PE by affecting coagulation.

Objective To learn current status of elderly depressive disorders in Yuetan community residents to provide evidence for policy making.MethodsCluster sampling method was used to enroll 352 elderly individuals from Yuetan Community for Geriatric Depression Scale(GDS)and self-designed questionnaire.Results The morbidity of elderly depressive disorder was 23.86%(84/352).The awareness rate of risk factor of elderly depressive disorder Was 4.83%to 42.90%.Conclusion The morbidity of elderly depressive disorder of Yuetan Community is higher,and related healthcare education should be strengthened.

Objective To investigate the effects of progesterone on apoptosis and Survivin expression protein of Ishikawa cell line of human endometrial carcinoma in vitro.Methods The proliferation of Ishikawa cells in vitro was determined by methyl thiazolyl terazolium (MTT) medhod.Cell cycle,apoptotic percentage were detected by cytometer. The expression of Survivin protein of Ishikawa cells was detected by immunocytochemistry. Results Progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone reached at 0.5?10-5～10-4mol/L(P

Objective To compare curative effects between laparoscopic oviduct-sparing operation and medical interventional treatment for the management of tubal pregnancy.Methods A total of 63 patients with tubal pregnancy were randomly divided into two groups.One group underwent laparoscopic salpingotomy and aspiration of the gestational sac content, while the other group underwent intra-tubal injection of methotrexate(MTX).Curative effects of the two procedures were compared.Results Laparoscopic oviduct-sparing operation was successfully performed on one session in 31 out of 32 patients,the success rate being 96.9%.Medical interventional treatment was conducted successfully in 24 out of 31 patients,23 of whom received one dose of MTX and 1 of whom received two doses,the success rate being 77.4%.There was no statistical difference between the two groups in success rates (?~2=3.765,P=0.052).The time for serum ?-hCG to normal levels was significantly shorter in patients receiving laparoscopic oviduct-sparing operation(9.1?2.1 d) than in patients receiving interventional treatment(26.3?3.9 d)(t=-20.974,P=0.000).Conclusions Both laparoscopic oviduct-sparing operation and medical interventional treatment can effectively preserve affected oviduct for patients with tubal pregnancy.As compared with medical interventional treatment,laparoscopic oviduct-sparing operation has a broader range of indications,less side effects,and a shorter recovery time of serum ?-hCG.

Nematoda anticoagulant protein c2((NAPc2)) is a protein originally isolated from the canine hookworm Ancylostoma caninum.NAPc2 binds to a noncatalytic site on fⅩa then selectively inhibits fVⅡa/TF complex.NAPc2 is proven to be a safe and potent anticoagulant in Phase Ⅰ and Phase Ⅱ clinical trials,and it has prolonged elimination half-life(longer than 50 h).In a word,NAPc2 may play a promising role in the prevention and treatment of thrombosis,disseminated intravascular coagulopathies(DIC) and tumor.

Objective To investigate the current status of self-management action of patients with chronic kidney disease (CKD) during early-mid stage and its influencing factors. Methods 80 patients were recruited in a tertiary hospital in Beijing from March to June 2011.They were surveyed with a self-designed self-management action questionnaire of patients with CKD. Results The total score of self-management action in patients with CKD was(69.10±5.63 ).1.25％ patients with CKD had bad self-management ability and 88.75％ patients had moderate self-management ability,only 10.00％ patients had good self-management ability.Patients of younger age,with higher education level,ever attending lectures or having inpatient experience had better self-management ability.And the methods of payment also had significant influence on the self-management of patients with CKD. Conclusions When taking care of patients with CKD,health care providers should be more individual case-based,especially paying more attention to those who are older,with low education degree,never have attended health education lectures and do not have inpatient experience,in order to increase patients' self-management ability and help them improve their health status.

Objective To investigate the function and possible action mechanisms of microRNA hsa-mir-634 in Vero cells.Methods The binding sites for hsa-mir-634 in the 3' UTR of cyclin D1 (CCND1) were predicated by bioinformatics methods.Then,the 3'UTR sequence of CCND1 containing the binding sites for hsamir-634 was amplified by PCR.Site-directed mutagenesis was used to create mutations in the binding sites.The wild and mutant 3' UTR sequences of the CCND1 gene were ligated into the psi-CHECK2 vector separately to construct dual-luciferase reporter vectors,including CHECK2-CCND1 wild,CHECK2-CCND1 mut 1,CHECK2-CCND1 mut 2 and CHECK2-CCND1 mut 3.Then,293T cells were transfected with the four constructed plasmids,and luciferase activity was measured 48 hours after the transfection.Vero cells were transfected with hsa-mir-634 mimics and negative control separately,and harvested after additional culture for different durations; the Vero cells remaining untreated served as the blank control.Subsequently,fluorescence-based quantitative PCR and Western blot were performed to detect the mRNA and protein expressions of CCND1 respectively in,3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,inner salt (MTS) assay to evaluate the proliferation of,and flow cytometry to detect the apoptosis in,Vero cells.Results The binding sites for hsa-mir-634 in the 3'UTR of CCND1 were successfully predicated.Sequencing results showed the successful construction of dual-luciferase reporter vectors.As the luciferase assay revealed,the overexpression of hsa-mir-634 could significantly inhibit the CCND1 3'UTR-mediated luciferase activity.Compared with the negative control,the hsamir-634 mimics markedly decreased the protein expression of CCND1,but had no obvious effect on the mRNA expression of CCND1 in Vero cells.The proliferation of Vero cells transfected with hsa-mir-634 mimics was significantly restrained compared with those transfected with the negative control,and the strongest restraining effect was observed on day 4 after the transfection.In addition,the overexpression of hsa-mir-634 also induced the apoptosis of Vero cells,with the apoptosis rate being 8.03％,7.96％ and 17.33％ in the blank control group,negative control group and mimics group respectively.Conclusion Hsa-mir-634 may regulate the proliferation and apoptosis of Vero cells via influencing the expression of CCND1.

Objective To observe the occurrence of vascular calcification in maintenance hemedialysis (MHD) patients. Method Sixty-nine MHD patients were enrolled in this study. Vascular calcification was evaluated by plain X-my films of chest, upper and lower extremites. Analyzed the relationship of vascular calcification and cardiovascular disease. Results Vascular calcification on X-ray films presented in 38 cases (55%). Of the 23 cases with calcification on arteries of extremites, 14 cases were found to have both intimal calcification and medial calcification. Patients with vascular calcification were older. Ischemic heart disease and cardiac failure appeared more frequently in the patients with vascular calcification. Conclusions Both intimal calcification and medial calcification are frequently found in the MHD patients. Vascular calcification is probablly associated with cardiovascular disease in MHD patients.

Objective: To examine the relationship between parental rearing and adolescent personality traits. Methods: A sample of 637 adolescents was tested by CAPI and EMBU. Results: Neuroticism of girls is higher than that of boys, but interpersonal harmony, face and defensiveness of boys is higher than those of girls. Extraversion, openness and agreeableness of only child is higher than those of other adolescents. Most correlations between parental rearing and Adolescent personality traits are significant. Parental warmth and understanding have positive effect on adolescent extraversion, agreeableness and conscientiousness, and father punishment and mother refusal have negative effect on adolescent agreeableness and interpersonal relationship. Conclusion: Adolescent personality is greatly affected by parental rearing.

Objective To study the operating approaches of laparoscopic resection of ovarian benign cystic teratomas. Methods 67 patients underwent operative laparoscopy for ovarian cystic teratomas(23 ovarian cystectomies,44 oophorectomies or adnexectomies),including 27 patients who had cysts more than 8cm in diameter.In operation, a plastic bag was used and the abdominal cavity was abundantly flushed with warm physiologic saline.The larger cysts were aspirated to reduce the cysts and extracted through abdominal wall incision. Results Cyst rupture occurred in 17 cases (17/44) when cystectomy was performed. No cyst spillage occurred in all the oophorectomies and adexectomies.The mean operating time was (34?19)min.Blood loss was 3ml～7ml.No complications occurred and none of the patients needed conversion from laparoscopy to laparotomy.chemical peritonitis wes not seen in any of the patiants. Conclusions Laparoscopic surgery is safe and feasible for ovarian benign cystic teratomas and allows the removal of larger ovarian cysts.