Objective In order to understand the characteristics and the distribution of HIV infection among voluntary blood donation population by analyzing the result about anti -HIV confirmed positive blood of the blood donors.Methods In the study,researchers used ELISA three and four generations of reagents to carry out anti -HIV blood test,and used the Western Blot to confirm the result.Results 176 024 cases of blood collected from voluntary blood donors had been made anti -HIV blood test,between June 1999 and June 2014.The result showed that 274 cases were testing positive,accounting for 0.16%,and 27 cases were confirmed positive,accounting for 0.015%. All the confirmed positive bloods were HIV -1 type virus infection.In the confirmed positive,23 male patients accounted for 85.2%,and it was greater than the proportion of women;age ranged from 19 to 30 years (16 cases, accounting for 59.3% of the total number;professional farmers in 13 cases accounted for 48.2% and it was bigger than other occupations;The cultural degree was less than college level;The regional distribution was mainly in Yuci in 9 cases,each of the remaining counties each accounted for 1 ~2 cases,3 cases in the field of mobile staff,students, migrant workers in 1 case.Conclusion The number of confirmed positive is rising up year by year,and the distribution of HIV infection population is widespread and spread from high -risk groups to the general population. According to the recent situation,the author suggests to ensure the blood safety,it is necessary to do that increasing the publicity and recruitment about the voluntary blood donation,and strengthening blood screening efforts.

Objective:To understand suicide prevention knowledge, suicide attitude and related factors among undergraduates in Changsha.Methods:Using multistage stratified cluster sampling, a total of 1664 undergraduates from six universities in Changsha were tested with Suicide Awareness Questionnaire, Suicide Attitude Questionnaire (QSA), Adolescent Self-Rating Life Events Check List (ASLEC), Social Support Questionnaire (SSQ) and Symptom Checklist 90 (SCL-90). Results:The mean score of suicide awareness among undergraduates was 10.8?0.9 and the correct response rate was 54.5%. The result of suicide awareness score showed, female undergraduates had a lower score than males (9.2?0.8, 12.3?0.8;t=2.33, P=0.020), medical undergraduates had a higher score than ones of humanities and social science or engineering (13.9?0.8, 10.0?1.0, 9.7?0.7;F=10.47, P=0.000), undergraduates from one-child families had a higher score than ones from non-one-child families (11.9?0.7, 9.1?0.9;t=3.25, P=0.034), and college students from nuclear family had a higher score than ones from extended families or one-parent families (14.0?0.9, 10.3?0.8, 9.4?0.7;F=7.49, P=0.000). The major factors related to suicide awareness were gender, specialty and family type (?=0.087, 0.342 and 0.084, respectively). And the major factors related to attitude towards attributes of suicide behaviors were specialty, gender, suicide ideation and mental health status (?=0.688, -0.055, 0.810 and 0.731, respectively). Conclusions:College students in Changsha have severe misunderstands about suicide prevention. It is necessary to pay more attention to the education of suicide knowledge and to cultivate the correct suicide attitude among college students.

OBJECTIVE To study the hospital infection among surgical system inpatients in order to work out more effective management plans concerning hospital infection.METHODS Totally 5963 surgical patients from Dec 2000 to Nov 2002 were analyzed by means of the retrospective study.RESULTS Among them there were hospital infections in 243 cases.The surgical hospital infection rate was 4.08% and the neurosurgical hospital infection rate was 5.37% which was the highest among all departments.The main part of infection mostly located in lower respiratory tract(21.30%),then were higher respiratory and urinary tracts(9.88%),incision site(10.70%),gastrointestinal tract(9.05%) and the others(6.17%).The infection rate in male patients was higher than in the females.The sex ratio was 2.2∶1.The infection rate was 4.67%.The highest infection rate after admission in hospital and at 31 to 60 days from January to March(4.95%) as well as from October to December(4.58%).The bacteriological rate was 28.40%,lower than the rate of≥50.00% demanded by the Ministry of Health.CONCLUSIONS The hospital infection in surgical patients is closely related to the departments,sex,duration of hospital stay,season and invasive clinical procedures.

ObjectiveTo investigate the effect of surrounding-acupuncture on scalp on hemiplegia.Methods90 patients were divided into three groups. First group were treated with surrounding-acupuncture on scalp, second group with traditional scalp-acupuncture, the third group was control group. ADL and Mannal Function Test (MFT) were used to evaluate the function pre- and post treatment. ResultsThere were no significant differences among the three groups on ADL and MFT at beginning, that were improved significantly after treatment. However, the first group improved more significantly than that of the other two groups for ADL and low limb MFT. The first group showed significantly improvement for Hamilton Rating Scale for Depression pre- and post treatment.ConclusionThe surrounding-acupuncture on scalp is effective method for hemiplegia.

Objective To investigate the method of disinfection and sterilization of ventilator tubes and accessories in order to increase the sterilization quality of them. Methods 120 ventilator tubes and accessories from ICU and emergency department were randomly dividied into the control group,and the study group. Bacteriologic tests were conducted in the two groups. Results There was significat differ-ence in bacteriologic tests between the two groups. Conclusions It is necessary to research into steriliza-tion method of ventilator tubes and accessories.

Objective To study the influence of sunscreens with different efficacy on delayed type hypersensitivity (DTH) and their immunoprotective effect in mice.Methods A cohort of mice were randomly divided into 5 groups with 10 mice in each group:group 1 as the positive control without irradiation,group 2 receiving solar-simulated radiation (SSR) only,group 3 receiving SSR and protected by sunscreen l with sun protection factor 15(SPF15)and persistent pigment darkening(PPD)12,group 4 receiving SSR and protected by sunscreen 2 with SPF 50 and PPD 28,and group 5 as the negative contml receiving SSR only.SSR was carried out on the back of mice with the UVA dose being 1.4 J/cm2 and UVB dose being 100 mJ/cm2 for 10 days.After a 5-day irradiation,the groups 1 to 4 were immunized by intraperitoneal injection with 100 μl(107 cells/ml) of Candida albicans suspension.On the 10th day both sides of the posterior foot pad were measured;then the foot pads were injected with additional 50 μl of the Candida albicans suspension.Twenty-four hours after the injection,the thickness of each foot pad was measured,and immunosuppression rate was calculated.Finally,the mice were sacrificed and skin samples were obtained from the back of these mice followed by the examination of CDla, CD80 and CD86 expression by Western blot.Resets The thickness of edema in foot pads was 0.41±0.38 mm,0.21±0.23 mm and 0.30 ± 0.25 mm in group 1,3 and 4,respectively,significantly higher than in group 5 and 2(0.04±0.03 mm,0.14±0.12 mm,respectively,all P<0.05),while no significant difference was observed between the group 3 and 4(P>0.05).Significant differences were observed in the immunosuppression rate between group 2,3 and 4(73.0%±11.3%,54.1%±6.4%,29.7%±7.5%,respectively,all P<0.01).Western blot revealed a significant increment in the expression of CDla protein in group 1 compared with group 2 as well as in the expression of CD86 protein in group 1 and group 3 compamd with group 2 and group 5(all P<0.05),but no statistical difference was observed between the other groups in the expression level of CDla,CD80 or CD86(P>0.05).Conclusions The exposure to sub-erythema dose of UV can induce DTH,and sunscreens have an immunoprotective effect in this process.Epidermal Langerhans cells are not essential for UV-induced immunosuppression.

Objective To explore the characteristics of blood oxygen level dependent (BOLD) magnetic resonance imaging (MRI) in healthy native kidneys. To investigate the relationship between BOLD-MRI and common physiological in-dexes. Methods GE 3.0T MRI scanner and Torsopa phased-array coil were employed to acquire renal coronal T1WI and BOLD image. Ninety patients who were ruled out chronic kidney diseases underwent BOLD-MRI with T2*-spoiled gradient recalled echo (T2*SPGR) sequence. BOLD images were analyzed on R2*map software. Cortical and medullary R2*values were analyzed in bilateral kidneys and in different gender. Different regional R2*values in cortex and medulla were also ana-lyzed. Physiological indices including age, body height and weight, body mass index (BMI), body surface area (BSA), estimat-ed glomerular filtration rate (eGFR) were recorded. Correlation between R2*value and physiological indices were analyzed. Results Bilateral renal cortical R2*values [left (16.56±1.40) Hz and right (16.66±1.28)Hz, respectively] were less than val-ues in medulla [left (28.82±3.71)Hz and right (28.36±3.72)Hz, respectively]. Female and male cortical R2*values [female (16.55±1.30Hz) and male (16.66±1.38)Hz, respectively] were also less than corresponding values in medulla [female (28.46± 3.64) Hz and male (28.70±3.78) Hz, respectively]. In bilateral renal medullary region, R2*values in low pole (27.29±3.05) Hz was less than values in middle (29.32±3.47) Hz and upper pole (29.16±4.21)Hz (F=15.184, P<0.001). Age was positive-ly correlated with R2* values in medulla (r =0.284, P =0.002). However, eGFR was negatively correlated with medullary R2*value (r=-0.232, P=0.007). Conclusion R2*values reflected the levels of renal partial pressure of oxygen and as-sessed the degree of renal ischemia. BOLD MRI could offer a simple, convenient and noninvasive method for to evaluate renal oxygen metabolism in cortex and medulla.

Objective To evaluate whether S-100β protein could be a serum marker for traumatic spinal cord injury (SCI). Methods From June, 2013 to October, 2014, 24 patients with complete SCI were measured the serum S-100β protein concentrations with en-zyme-linked immunosorbent assay, one week, three and six months after SCI. Serum from ten healthy persons was as normal control. Re-sults The serum S-100βprotein concentrations increased one week and 3 months after SCI (Z>4.273, P<0.001). Conclusion The increase of serum S-100βprotein may help assessing early impairment after complete SCI.

Objective To study the relationship between the asthenia TCM syndromes in embryonic development stops and the level of zinc, iron, copper, magnesium, phosphorus, calcium in serum, and provide thoughts for assisting reproduction and preventing miscarriage. Methods Totally 100 patients of embryonic development stops were selected randomly (6-10 weeks gestation) to be the investigated group, with other 100 cases of live fetus as the control group. The contents of trace elements in serum were detected with atomic absorption spectrometry. Results The levels of trace elements in embryonic development stops patients were generally lower than the control group. The serum iron in patients with spleen deficiency syndrome, and serum zinc and iron in patients with kidney deficiency syndrome were significant lower (P<0.05). Conclusion The contents of trace elements in serum have relationships with asthenia TCM syndromes in embryonic development stops. It should be paid attention to supplementing trace elements during the gestation period. For patients with deficiency of kidney and spleen, the supplement of zinc and iron should be given greater prominence.

Objective:To ascertain the distribution of interventional radiodiagnosis and treatment resources and the current situation of radiological protection in Beijing, standardize the interventional radiodiagnosis and treatment, and promote the implementation of radiation protection regulatory measures.Methods:Various medical institutions at differetn levels that perform interventional radiodiagnosis and treatment in Beijing were identified as the survey objects. With special questionnaires designed, the investigation groups at two levels of municipality and district was established to investigate the basic situation of interventional radiology and occupational health monitoring by the end of 2020. The indexes and parameters were analyzed and evaluated under the relevant national regulations and standards.Results:By the end of 2020, there were 93 medical institutions performing interventional radiology in Beijing, together with 236 digital subtraction angiography machines (DSA) with higher than 800 mA. A total of 135 593 cases of interventional radiological surgical operation were performed. There were 40 hospitals annually performing more than 1 000 cases and 41 hospitals perfoming 10-1 000 cases. There were 3 539 interventional radiological workers, with 99.0% holding radiological worker certificates. The passing rates of occupational health examination, personal dose monitoring and radiation protection training were 96.9%, 99.5% and 95.8%, respectively. A total of 3 532 sets of protective articles were provided for the workers, of which 98.9% were equipped with split or integrated lead clothing, but 6.5% were not equipped with lead protective glasses and 54.9% were not equipped with lead protective gloves.Conclusions:The radiation protection management for the interventional radiodiagnosis and treatment is generally good. However, the regulatory mechanism should be further improved based on the current distribution of interventional radiodiagnosis and treatment resources, with focus on strengthening the occupational health examination, the radiation protection training, and the configuration and use of protective equipment.