Acupuncture Points ; Adult ; Aged ; Female ; Ginger ; chemistry ; Humans ; Male ; Middle Aged ; Moxibustion ; Stroke ; complications ; Urinary Incontinence ; etiology ; physiopathology ; therapy ; Urination

Obesity and its related metabolic diseases become major health problems in the world.Adipose tissue plays an important role in the development of obesity.FSP27,a member of the CIDE family proteins,is expressed at high levels in white adipose tissue and differentiated 3T3L1 cells.The objective of current study is to establish a FSP27 knockdown preadipocyte cell line to investigate the in vivo function of mouse FSP27.The double strand siRNA of mouse FSP27 corresponding to nucleotides 270 to 291 was synthesized and inserted into pSilencer2.1.pSilencer-siFSP27 was co-transfected into 293T cells with the HA-mFSP27 expression vector to test its knock-down efficiency.The FSP27 siRNA was then transferred to a lentiviral vector.Lentivirus were generated and used to infect 3T3-L1 cells.It was shown here that lentivirus containing FSP27siRNA can effectively knockdown FSP27 expression in 3T3-L1 cells.Establishment of FSP27 knock-down cell line provides a useful tool for the study of in vivo function FSP27.

This paper detailed the Coleman concept and practice of coach-led transitional care of elderlies,team members and their responsibilities,intervention aspects and main tasks,as well as the intervention program and its effect analysis.Then the authors analyzed the present transitional care development in China,and recommended on development of a likewise care model for senior chronic disease patients in the country,such as building multi-disciplinary transitional care teams to provide uninterrupted care and service,encourage patients and caregivers to embrace self-management of diseases,and build an appraisal mechanism to ensure quality of care.

Cognitive impairment after stroke is one of the common problems, that affects the physical, behavioural and emotional rehabilitation, etc. There is no effective means of Western medicine. This article reviewed understanding, medication, acupuncture and moxibustion for cognitive impairment in view of Chinese Medicine.

This paper summarized the epidemiology and management of Chinese andWestern Medicine for voiding disorders.

The spasticity can affect the motor function, activities of daily living (ADL), quality of life (QOL) of hemiplegic patients,and also delay the rehabilitation process. Principally, the methods of reducing spasticity included physical therapy and medication at present.The massage technique has been having outstanding effect on alleviating spasticity after stroke. This article reviewed the clinical efficacy,methods and mechanisms of massage treatment for hemiplegic spasticity.

Objective To study the relation between the clinical feature and glycoprotein D gene sequence analysis of a wild strain of HSV 2 isolated from one relapsed patient with genital herpes. Methods The partial glycoprotein D gene sequence of the above mentioned strain was amplified and cloned with PCR. Results The comparison of the amino acid sequence of gD gene between the wild strain and HSV 2G strain published showed that there was a mutation at site80 and site159. Conclusion In order to develop effective vaccine which is suitable for China, it is necessary to investigate the differences of the gene structure and function of gD among HSV isolated from China and other countries.

Objective: To study the corrosion resistance of orthodontic magnet with titanium nitride coating in artificial saliva. Methods: 6 samples of magnet in the size 3 mm?5 mm?7 mm were prepared.3 of them were coated with titanium nitride by plasma enhanced chemical vapor deposition,another 3 without coating were used as controls.The samples were immersed into artificial saliva at 37 ℃ for one month. Then the surface characters of two kinds of magnets were observed with the naked eye and by electron-probe microanalysis (EPMA). Results: The surface of the magnets with titanium nitride coating was fine and close in texture and there were no cleft and disintegrated area. However, that of the magnets without titanium nitride coating was uneven and coarse in texture,and there were evident clefts and disintegrated areas. Conclusion: The corrosion resistance of orthodontic magnets with titanium nitride coating is stronger than that without coating.

Objective: To study the corrosion resistance of the ortho do ntic magnets with titanium nitride (TiN) coating. Methods: The m agnets coated TiN film were immersed into artificial saliva at 37 ℃ for two mon ths. During the immersion, the concentration of iron ion in the artificial saliv a was analyzed with atomic absorption spectrophotometry(AAS). After immersion, t he weight change of the magnets was measured.The magnets coated with nickel film and without coating were used as the controls.Results: In all t he groups, iron ion concentration in the artificial saliva increased with the in crease of immersion time(P

Objective:Comprehensive evaluation on the puerperal women's health status,and probing those influencing factors on their quality of life. Methods:513 puerperal women received the survey on health status including physical, psychological and social function dimensions by relevant items of Quality of Life designed by WHO and related factors were analyzed by stepwise regression.Results:The QOL of puerperal women were lower than general women aged between 18-49 years old in three dimensions(91.44?4.05/97.84?3.66,u=25.95,82.42?7.02/89.81?8.50,u=17.53,88.87?4.99/98.12?4.59,u=29.47,均P