Recently, the incidence of both diabetes mellitus and diabetic foot has been increasing.Ischemia, neuropathy, and infection are major causes of diabetic foot ulcer. In addition to other conventional treatments, hyperbaric oxygen is an effective adjunctive therapy with less side-effects. Hyperbaric oxygen may play a role through different mechanisms in improving the prognosis of diabetic foot.

When the tracheotomy and tracheal intubation patients were treated in hyperbaric oxygen chamber,not ordi-nary facemask but specially made therapeutic facilities should be used.To select a more reasonable therapeutic facility for patients treatment in HBO chamber,all kinds of hyperbaric oxygen therapeutic facilities for the tracheotomy and tracheal intubation patients of our country in recent years are introduced from the aspects of material,facility,impermeability,ther-apeutic efficiency,influences on oxygen concentration in chamber,the advantages and disadvantages of each kind were demonstrated.

Objective:To observe the effect of timosaponin AⅢon the proliferation of cultured melanoma B16 and A375 cells and nitrite produced from macrophage activation. Methods:MTT assay was adopted to detect the cell growth inhibition. The morphological changes of B16 and A375 cells were observed under an inverted microscope. Nitrite production of activated mouse macrophages induced by lipopolysaccharide ( LPS) plus IFN-γ was measured by Griess assay. Results: Timosaponin AⅢ could significantly inhibit the growth of B16 cells at the concentration of 16 μmol·L-1 after the 48- and 72-hour treatment, and significantly inhibit the growth of A375 cells at the concentration ranged from 4 to 16 μmol·L-1 after the 48-and 72-hour treatment. Shrinkage, vacuoles and necrosis of B16 and A375 cells were observed after the 48-and 72-hour treatment by 16μmol·L-1 timosaponin AⅢ,the other concentration of timosaponin AⅢ showed no notable effect on B16 cells and vacuoles of A375 cells appeared at the concentration from 4 to 16 μmol· L-1. Compared with RAW 264. 7 stimulated LPS plus IFN-γ,timosaponin AⅢ could significant inhibit nitrite production of macro-phage inflammatory cell model at the concentration of 10μmol·L-1(P<0. 01). Conclusion:Timosaponin AⅢcan inhibit the prolif-eration of melanoma B16 and A375 cells and macrophage inflammation,suggesting it has anti-tumor and anti-inflammatory effects . The anti-tumor effect of timosaponin AⅢ may be related to the inhibition of tumor inflammation.

Objective To discuss the apparatus of inspired oxygen in hyperbaric oxygen(HBO)chamber for patients after tracheotomy.Methods Many kinds of apparatus of inspired oxygen were analyzed,including composition,usage and characteristics.Results The apparatus of inspired oxygen play an active role in treating patients.But in the above apparatus,there are great differences in serving range,efficiency of inspired oxygen,curative effect,reliability and so on.Conclusions When serious patients after tracheotomy are treated in HBO chamber earlier,it is very important to select reasonable apparatus.

This paper introduces the current situation of hyperbaric oxygen (HBO) therapy, the environment characteristics in HBO chamber and the qualification of sputum aspirators. What's more, this paper also describes the construction, principles and characteristics of all kinds of sputum aspirators in HBO chamber. When critical patients are treated in HBO chamber, it is very important to select reasonable sputum aspirators.

Objective To investigate the effect of ellagic acid on human epidermal melanocyte melanogenesis and melanin transfer, and the mechanism thereof. Methods The human melanocytes and and keratinocytes were co-cultured and purified. After passing the second generation, cells of 1∶10 ratio were inoculated into the small dish (3 cm × 3 cm). The changes of melanin content and tyrosinase activity in melanocytes were detected before and after intervention with ellagic acid (100, 10 and 1 mg/L) for 48 h. The melanin transfer in cultured cells was detected by flow cytometry method. The 10 nmol/L arbutin was used as the positive control. Results The tyrosinase activity was down-regulated by ellagic acid in a dose-dependent manner. The ellagic acid can reduce the melanin content except for the 1 mg/L of ellagic acid. The melanin transfer was also inhibited by ellagic acid in a dose-dependence manner. Conclusion Ellagic acid can be used for skin-whitening cosmetic and the depigmenting effect might be due to the down-regulation of melanogenesis and melanin transfer.

In Scientific Research Institutions,it has great significance of the S&T resources archiving and sharing to promote the deepening reform of S&T system and realize the great leap-forward development of S&T innovation.In this paper,we conduct empirical analysis on the conditions of S&T resources archiving and sharing in a Medical Organization in Beijing.The results show that the S&T resources archiving and sharing in Medical Organization have achieved initial success,however,the achievements are not ideal and the waste and shortage of S&T resources still coexisted.In view of the deficiencies of the idea,system,laws and regulations of S&T resources archiving and sharing,this paper puts forward corresponding countermeasures and suggestions.

Objective To investigate the application and administration of hyperbaric oxygen chambers in Beijing.Methods Forty-eight hospitals with hyperbaric oxygen chambers in Beijing were investigated by field investigation,letter investigation or telephone investigation.The investigation includes grades of hospital,personnel,equipment,therapy of severe cases,time of therapy and treatment plans in questionnaire.Results Hyperbaric oxygen was popular in Beijing,which ensured emergency treatments.But the scale was little relatively and the number of full-time technicians was less,besides,the therapeutic system and plans were diversified,which should be standardized.Conclusions Something must be done to improve the conditions of hyperbaric oxygen medicine in Beijing,such as cultivating personnel,standardizing therapeutic system,standardizing treatment plans,realizing computer control and so on.

Objective To develop a gas dynamic exposure device with a simple structure.Methods The device was composed of a water tank, an gas chest, an exposure kit and an gas-out bottle. The chest body was made of organic glass pane, drainage method was used to distribute the gas properly, and airflow was regulated with the gravity and valve.Results The device was proved to have simple structure and high effects.Conclusion The device has low cost, simple structure and properly distributed gas, and can be reused for exposure test.

Ginsenoside Rb3 (GRb3) is one of the main components in plasma of Panax quinquefolius Saponin of stem and leaf (PQS), which can be into human plasma. Previous studies have found PQS has estrogen-like vascular protective effects. In the present study, we investigated the estrogen-like protective effect of GRb3 on oxidative stress and dysfunction of endothelial cells induced by oxidized low-density lipoprotein. The activities of SOD, NOS and the contents of MDA in the cell lysate were examined by enzyme method or spectrophotometry. The NO and ET-1 concentrations in the cell culture supernatant were measured by ELISA method. The iNOS and eNOS mRNA expression were measured by real time RT-PCR, while the phosphorylation levels of Akt was measured by Western blotting. The results showed that GRb3 could enhance the activity of SOD, reduce the content of MDA, increase the level of NOS, NO, ET-1 and iNOS mRNA expression while decrease the eNOS mRNA expression and the phosphorylation level of Akt. These effects were blocked by estrogen receptor antagonist ICI182780. GRb3 can play a role in protecting vascular endothelial cells by estrogen receptors, the protective mechanism is similar to 17-β estrodiol.