OBJECTIVE:To establish the quality standard of Sophora flavescens decoction.METHODS: The preparation was determined qualitatively using TLC,and the contents of matrine and oxymatrine were determined by HPLC.RESULTS: The TLC spots of S.flavescens decoction were clear and well-separated.The linear ranges of matrine and oxymatrine were 0.024 92～0.648 44 ?g(r=0.999 9) and 0.073 4～1.908 4 ?g(r=0.999 9).The average recoveries were 102.9% and 102.3% and RSDs were 6.9% and 102.3%,respectively (n=6).CONCLUSION: Establish standard can be used for the quality control of S.flavescens decoction.

Breast cancer is one of the most common malignant tumors in women. Bone is commonly affected in the context of metastatic breast cancer.Once bone metastasis happens, patient would experience poor prognosis and impaired quality of life.However, there is a lack of approaches for more sensitive and specific diagnosis and treatments for breast cancer bone metastasis.Thus, it is im-perative to find new treatment target from molecular mechanism.In this paper, we review the current research progress on the molecular mechanism from several levels including gene profile, proteins and microRNAs.We also review the establishment of animal models of breast cancer bone metastasis.With the achievements acquired in the completed or undergoing researches on breast cancer bone metasta-sis, we hope the finding of the optimal diagnostic and therapeutic targets could lead the breast cancer research into a new era.

Biofilms are communities of surface-associated bacteria or fungi embedded in a self-produced extracellular polymeric matrix that are notoriously difficult to be eradicated and are sources of many recalcitrant infections. Treatment for biofilm infection with any individual drug is always less effective, while the combinations of different types of drugs are superior to monotherapy concerning the removing of biofilms. This paper focus on research progress in recent years for synergistic effect of drugs in combination against biofilms formed by Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida albicans.

In line with the hospital development practice in recent years,this paper discussed the early-warning system for hospital talents drainage.First of all,it points out that early-warning indicators,early-warning analysis and early-control measures are key elements of such a system.On this basis,it pinpoints key points in building this system,including organizational assurance,by-level management,dynamic tracking,fairness,bettering of HR mechanism,and reservation of potential HR resources.Therefore,this will be highly useful and practical for studying how to minimize talents drainage and maintain competitive advantages of hospitals.

With the growing prevalence of obesity,diagnosis and treatment of obesity has drawn great attention.On the basis of previous guidelines on obesity,May 2016,American Association of Clinical Endocrinologists (AACE)and American College of Endocrinology (ACE)announced the clinical practice guidelines for comprehensive medical care of patients with obesity.The guidelines give advice and guidance in obesity diagnosis,assessment,treatment goals and treatment strategies around obesity-related complications as center.This article interprets the key content of the guideline in order to acquire enlightenment for clinical work.

Objective:To define the kinetics of soluble intercellular adhesion molecule 1 in serum levels after cerebral ischemia and their correlation with the clinical outcome, infarct volume as measured on CT scans ,and the relevance of vascular risk factors Methods:The levels of serum sICAM 1 were mesured with enzyme linked immunosorbent assay (ELISA) method in 35 patients with acute cerebral infaration and 20 cases of normal control Results:Serum sICAM 1's concentration in the samples of cerebral infarction on second day (P

Objective To explore predictive value of spectra parameter changing of transcranial Doppler (TCD) for intracranial pressure (ICP) and cerebral perfusion pressure (CPP) in patients with intracranial infection. Methods TCD examination was taken before 128 times of lumbar puncture for pressure testing in 42 patients with intracranial infection. Meanwhile, Data of TCD and cerebrospinal fluid (CSF) pressure were recorded for later analysis and compared with control group.Results With ICP increase progressively , the TCD of above patients showed high resistance spectra, waveform sharpened, peak systolic velocity (Vs) without significant change while in end diastolic velocity (Vd) decreased and pulsatility index (PI) increased.Compared with control group, their blood flow velocity significantly increased.According to the TCD parameter and stepwise regression equations, predicted ICP and CPP ( ICPe and CPPe) were positive correlated with actual ICP and CPP (r=0.594,0.910, allP

Objective To evaluate the effectiveness of the supportive group therapy on the psychological traumatic in the nurses who experienced a violence event.Methods 58 nurses were enrolled in this study and randomly arranged to therapy group and control group.Ninety minutes group psychotherapy was used in the therapy group once weekly and last 6 weeks,and no any intervention method was used in the control group.For both groups,evaluations were conducted at three time points that was baseline,immediately after the completion of the intervention and 3 months after the completion of psychotherapy.Evaluations were conducted using the Impact of Event Scale-Revised (IES-R) and Profile of Mood States (POMS).Results The reduction of the total scores of ISE-R and flashback factor,hyper-arousal factor,avoidance behavior factor in the therapy group were higher than those in control group (-5.00 (3.89) vs-1.48 (3.05),P ＜ 0.01 ;-1.53 (1.46) vs-0.60 (1.90),P ＜ 0.05 ;-1.97 (2.71) vs-0.18 (1.76),P ＜ 0.01 ;-1.50 (2.60) vs-0.70 (2.08),P ＜ 0.01,respectively).Both the reduction of total scores of PMOS and the tension-anxiety factor and depression-dejection factor scores on the POMS differed significantly between the two groups(-2.80 (19.40) vs-0.41 (14.05),P ＜ 0.01 ;-1.85 (3.64) vs 0.37(3.40),P＜0.01 ;-1.10(6.52) vs 0.13(4.30),P＜0.01,respectively).All these effects maintained 3 months after the psychotherapy completion.Conclusion The group psychotherapy can alleviate the psychological traumatic of nurses experienced violence events,and it can be used to protect the mental health of these nurses.

Objective To investigate the essential points of the diagnosis and the surgical treatment of malignant gastrointestinal tumors in children.Methods Clinical data involved the sites and pathological types of the tumors,clinical manifestation,effective treatment and prognosis were retrospectively analyzed in 29 consecutive patients reffered to Beijing Children's Hospital between January 1996 and May 2008.Results Of 29 patients,26 were males and 3 were females(aged from 1 year and 10 month to 14 years,mean age of 10 years).Gastric tumor was found in 5 cases (interstitialoma in 1,lymphoma in 2,endodermal sinus tumor in 1 and P-J syndrome canceration in 1),whereas intestinal tumor was found in 24 cases(malignant Iymphoma in 17,colon carcinoma in 4 and carcinoid of appendix in 3).The main clinical manifestations were abdominal pain,emesia,fever,alimentary tract hemorrhage and emaciation.Surgical complication involved perforation,internal fistula,obstruction,invaginate and hemorrhage.Twenty-four patients underwent(gaster)intestine resection and anastomosis.Biopsy was performed in 5 patients.Two patients with colonic carcinoma relapsed 6 months to 1 year after operation.Of 29 patients,17 patients survived,6 patients missed the follow-up and 6 patients died.Conclusions Pediatric malignant gastrointestinal tumor is rare.The unspecific presentation makes the diagnosis difficult.In diagnosis of acute abdomen and gastrointestinal tract diseases,the possibility of malignant tumor should be reminded.The imaging and endoscopic examination are very important to diagnosis.The surgical resection or combined with chemotherapy are mainly used in treatment of tumors.The prognosis is related to the pathological nature of the tumor.

Sox-2(SRY related HMG box-2)is an essential embryonic stem cell gene and a necessary transcription factor for maintaining the proliferation and self-renewal capability of embryonic stem cell .Sox-2 is involved in complex cell signal transduction pathway network and adjusting biological behavior of cells .Expression of Sox-2 is adjusted by different factors in transduction , transla-tion and post-translation levels .In recent years , increasing evidences indicate Sox-2 has an important role in tumor development , growth, proliferation, and metastasis, which is involved in various biological behavior of tumor cells in many types of tumors .This pa-per reviews the role of Sox-2 in adjusting tissue and tumor development and complex cell signal transduction pathway network of Sox -2 and clinical values of Sox-2 in tumor diagnosis and treatment until now .