Objective To establish a HPLC method of determining chrysin and baicalein in Senmen Oroxyli. Method The contents of chrysin and baicalein were determined by HPLC. The chromatographic conditions were: Kromasi1-C_(18) column (5 ?m, 250 mm?4.6 mm), with methanol-acetonitrile-water-glacial acetic acid (25 : 25 : 50 : 0.2) as the mobile phase, at the flow rate of 1 mL/min, the detecting wavelength at 275 nm and at the room temperature. Results The contents of baicalein and chrysin could be detected by this HPLC method, and got good separation and good linear relationship. RSD were 1.53% and 1.35%, indicating good repeatability, recovery RSD were 1.85% and 1.59%, indicating a good recovery. Conclusion The method is simple, feasible and repeatable, and can be provide reference for quality control of Senmen Oroxyli.

OBJECTIVE: To explore the influence of psychological factor on asthma, the protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms. METHODS: Allergy and stress-induced asthma models were established in rats by giving ovalbumin and restraining stress. Radioimmunoassay, enzyme linked immunosorbent assay, in situ hybridization and immunohistochemical method were used to detect plasma corticosterone, IL-4, IFN-gamma, expression of glucocorticoid receptor (GR) mRNA in hippocampus and adrenocorticotropic hormone corticoliberin (CRH) positive neuron in hypothalamus. Optical and electron microscopes were used to observe the morphological changes of pulmonary hilar and hippocampal tissues. RESULTS: Shugan Lifei Recipe (SGLFR) could reduce plasma corticosterone, decrease CRH positive neurons in paraventricular nucleus of hypothalamus, up-regulate the expression of GR mRNA in hippocampus and reduce the injury of hippocampal neuron. SGLFR had certain inhibitive effect on hyperfunction of hypothalamo-pituitary-adrenal cortex (HPA) axis in rats with asthma under stress and could also relieve the pathological changes of the pulmonary hilar tissue. The level of plasma IFN-gamma was increased while the level of plasma IL-4 was decreased in SGLFR-treated group. CONCLUSION: The mechanism of SGLFR in treating the rats with asthma under stress is probably to regulate the hyperfunction of HPA axis and the disorder of immuo-system.

Objective To investigate different dosage of dexmedetomidine for preventing elderly postanes-thetic shivering.Methods 100 ASAⅠ~Ⅱ elderly patients (60~80 ages)scheduled for laparotomy were enrolled in this study.According to a computer-generated list of random numbers,patients were randomly allocated to receive dexmedetomidine in the four groups:group A (0.9%normal saline),group B (dexmedetomidine 0.25μg/kg),group C (dexmedetomidine 0.5μg/kg),group D (dexmedetomidine 0.75μg/kg).The occurrence of postanesthetic shive-ring and adverse reactions in perioperative period and post operation were observed.Results The incidence rate shiv-ering were 24% and 20% in group C and D,which were significantly lower than 64% in group A (χ2 =5.333,6. 876,all P<0.05),but there was no difference between C and D groups(χ2 =0.117,P>0.05).Rectal temperature was no difference among four groups(F=1.271,P>0.05).Extubation time was shorter in group A(21.6 ±4.0)min than that in group D(24.4 ±2.8)min(F=6.194,P<0.05).Conclusion Our results suggest that dexmedetomi-dine 0.5 or 0.75 μg/kg can prevent shivering after general anesthesia effectively.But potential for delayed extubation time with dexmedetomidine was noted.The loading dose of 0.5 μg/kg dexmedetomidine is recommeded to prevent eld-erly shivering after general anesthesia.

Objective To explore the relation of skin carotenoid levels with lifestyle.Methods Complete clinical data of 493 adults who received health check-up in our hospital between December 2010 and December 2011were collected for this retrospective study.The relationship between skin carotenoid level and lifestyle was investigated.ResultsNo strong relationship between skin carotenoid level and age,education level,occupation and marriage status was found ( x2 values were 2.150,6.443,4.560 and 2.028,respectively; all P ＞0.05 ).However,skin carotenoid was significantly correlated with dietary variety,intake of fruits and vegetables,exercise frequency,cigarette smoking,passive smoking,mood,sleeping and gender( x2 values were 520.234,89.936,4.002,81.428,154.271,84.871,11.546 and 2.150,respectively ; all P ＜0.05 ).Conclusions Our data suggest a relationship between lifestyle and skin carotenoid level.Skin carotenoid assessment may help to detect bad lifestyle in time.

Objective To investigate the current status of moral distress in clinical nurses,and analyze its influential factors.Methods A total of 240 clinical nurses were recruited by stratified sampling method.They were investigated with general situation questionnaire and moral distress scale.Results The average score of moral distress was (2.66±0.41).Multiple linear regression analysis showed that department of work and qualification were influencing factors on moral distress of clinical nurses.Conclusions This study found that nurses' moral distress was above the middle level.Some targeted measures should be taken by nursing managers,in order to help decrease nurses' moral distress.

At present, long-acting interferon combined with ribavirin is still a major therapeutic method for hepatitis C in China, and precision medicine can help cure more patients with hepatitis C. This article briefly introduces the diagnosis and treatment of hepatitis in China and the importance of the strategy of virologic response-guided therapy (RGT), and analyzes the influencing factors for the achievement of sustained virologic response. HCV RNA quantitation is the foundation of RGT strategy, and HCV RNA quantitation with a high specificity performed at crucial time points during treatment helps to evaluate response accurately, realize the individualized treatment of hepatitis C, and improve the cure rate of hepatitis C.

OBJECTIVETo establish a SPE-HPLC method for the determination and pharmacokinetic study of evodiamine and rutacarpine in rat plasma.

METHODA Kromasil C18 column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-water-tetrahydrofuran-acetic acid (51:48:1:0.1) as a mobile phase and at a flow rate of 1 mL x min(-1), and the UV detection was at 225 nm. The column temperature was 35 degrees C. After the analytes were extracted from the plasma of rats by solid phase extraction (SPE), the content of evodiamine and rutaecarpine was measuared by HPLC method using halcinonide as an internal standard solution.

RESULTAfter transdermal administration to rats, the pharmacokinetic behavior of evodiamine and rutaecarpine belongs to the one-compartment model. The main pharmacokinetic parameters was as follows: K(a) 0.224 h(-1) and 0.220 h(-1), K(e) 0.114 h(-1) and 0.118 h(-1), C(max) 0.211 mg x L(-1) and 0.272 mg x L(-1), T(peak) 6.132 h and 6.102 h, respectively.

CONCLUSIONThe method in this study is simple, rapid and sensitive. It is proved to be suitable for pharmacokinetic study of evodiamine and rutaecarpine.

Animals ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; pharmacokinetics ; Indole Alkaloids ; blood ; pharmacokinetics ; Male ; Plant Extracts ; blood ; pharmacokinetics ; Quinazolines ; blood ; pharmacokinetics ; Rats ; Rats, Sprague-Dawley ; Solid Phase Extraction ; methods

Objective To evaluate the efficacy of the sublingual immunotherapy with Dermatophagoides farianae drops on different age groups. Methods The efficacy of 117 patients with AR received SLIT treatment for more than one year were retrospectively analyzed. These patients were divided into three groups according to the age,children group(6-14 year old,35 patients),youth age group(15-35 year old,45 cases)and middle-aged group(36-66 year old,37 pa-tients). Before and SLIT treatment for half one year, one year,symptom score, medication score and signs score were evaluated. Results After SLIT treatment for half a year and one year, the symptom score, medication score and signs score in these patients significantly reduced compared with before(P<0.05). Compared with after half a year treatment,the symptom score of children group significantly reduced(q=3.05,P<0.05),and other indicators were not statistically significant (P>0.05). The symptom score, medication score and signs score among three age groups were not statisti-cally significant (P>0.05) at all the time points. Conclusion Dermatophagoides farinae drops on allergic rhinitis can play a significant and similar effect in the different age groups.

Objective:To observe the effect of goal-oriented management of continuous monitoring of regional cerebral oxygen saturation (rSO 2) on early postoperative neurocognitive disorders (PND) in elderly spinal surgery patients. Methods:From November 2018 to July 2019, 60 patients undergoing posterior lumbar interbody fusion in the Second Hospital of Shanxi Medical University were selected and randomly divided into control group and intervention group, 30 cases in each group. RSO 2 was recorded before anesthesia induction (T 0), 10 min after anesthesia induction (T 1), 10 min after prone position (T 2), 10 min after spinal decompression (T 3), 30 min after spinal decompression (T 4) and 10 min after extubation (T 5); The basic value of rSO 2, the minimum value of rSO 2 (rSO 2min), the average value of rSO 2 (rSO 2mean) and the maximum percentage of decrease of rSO 2 (rSO 2% max) were recorded. When rSO 2 <55% or rSO 2% max >10% and the duration was longer than 15 s, the intervention group took measures such as adjusting head position, adjusting blood pressure, increasing FiO 2 and respiratory parameters, increasing P ETCO 2 until rSO 2 returned to the required range; The control group did not interfere with the intraoperative rSO 2. Neuropsychological tests were used to evaluate the cognitive function of the two groups 7 days after operation. The patients were followed up 30 days after operation with the revised cognitive function telephone questionnaire (TICS-M). The incidence of postoperative neurocognitive impairment (PND) was recorded. The perioperative data and postoperative adverse reactions of the two groups were recorded. Results:At T 3 and T 4, the rSO 2 of the intervention group was significantly higher than that of the control group ( P<0.01), the intraoperative rSO 2min and rSO 2mean of the intervention group were higher than the control group, and the rSO 2%max was lower than the control group ( P<0.05). The incidence of PND 7 days after surgery, extubation time, postanesthesia care unit (PACU) stay time, hospital stay and postoperative adverse reactions in the intervention group were lower than those in the control group ( P<0.05). There was no significant difference in the incidence of PND 30 days after operation between the two groups ( P>0.05). Conclusions:The goal-oriented management of rSO 2 can reduce the incidence of early postoperative PND in elderly spine surgery patients, which is conducive to the rapid recovery of patients after surgery.

Objective:To investigate the anxiety and depression of elderly patients with lung cancer before and after chemotherapy.Methods:A total of 168 elderly patients diagnosed with lung cancer and eligible for chemotherapy in a Grade A tertiary hospital in Shenyang from Jan 2022 to Feb 2024 were selected as the study subjects.The basic data,self-rating depression scale(SDS),and self-rating anxiety scale(SAS)scores before and after chemotherapy were collected.The Spearman rank correlation test was used to analyze the correlation of the general data of patients with SDS and SAS scores before and after chemotherapy.Results:SDS and SAS scores of lung patients after chemotherapy were significantly higher than those before chemotherapy(P＜0.05).The proportion of patients with depression and anxiety after chemotherapy was also significantly higher than that before chemotherapy(P＜0.05).Results of Spearman's rank correlation test showed that,the increase of SDS and SAS scores was correlated with gender,pathological type,chemotherapy cycle,combined underlying diseases,and respiratory symptoms(P＜0.05).Conclusions:Chemotherapy increases the proportion of anxiety and depression in elderly patients with lung cancer.Gender,pathological type,chemotherapy cycle,combined underlying diseases,and respiratory system symptoms are correlated with the occurrence of anxiety and depression.