AIM: To investigate the CPU86017 and its enantiomers inhibit abnormal gene expression of calcineurin and NFκB in rat cardiomyopathy induced by L-thyroxin and compare the effect of CPU86017 (racemate) with its 4 enantiomers: (7S, 13R), (7S, 13S), (7R,13S), and (7R,13R)-CPU86017 in this model. METHODS: The animals were randomly divided into 7 groups. The rat hypertrophied model was produced by treatment with L-thyroxin 0.2 mg·kg-1·d-1, sc for 10 d and treated with CPU86017 or its enantiomers 4 mg·kg-1·d-1, sc from d 6 to d 10. The changes in left ventricular (LV) weight index, redox system, and the NO and iNOS activity in the myocardium were investigated. The expression of mRNA of calcineurin、NF-κB in the left ventricle was measured. RESULTS: There were significant cardiac hypertrophy and oxidative stress in rats treated by L-thyroxin. The expression of calcineurin, NFκB mRNA were upregulated (P<0.05, compared with that of control). After treatment with CPU86017 (racemate and enantiomers), LV remodeling and the redox system were improved. CPU86017 and (7S,13R)-CPU86017 showed a better improvement on LV remodeling and the redox than the other isomers and restored the normal expression of calcineurin, NF-κB (P<0.05, P<0.01), respectively. CONCLUSION: It suggested that an up-regulation of calcineurin and NFκB possibly related to the altered intracellular calcium handling system plays a role in the progression of L-thyroxin induced cardiomyopathy and CPU-86017 and its 7S,13R-CPU86017 enantiomer effectively inhibit the abnormal expression of calcineurin and NFκB genes, the NOS enzyme and oxidant stress in the cardiomyopathy.

Objective To investigate the clinical value of prenatal MRI in the diagnosis of fetal bowel obstruction.Methods Pregnant women suspected to have fetal abdominal abnormalities by ultrasonography were suggested to undergo MRI examinations within two days.Scanning sequence included FIESTA,SSFSE and T1WI SPGR sequence,with field of view focused on the fetal abdomen.After the final diagnoses of the cases were obtained by induced labor pathological examination or postpartum imaging or operation,the imaging data and the clinical data were reviewed and analyzed retrospectively.Results A total of 23 cases with bowel obstruction were included in the study.Four fetuses with duodenal atresia showed low T1 signal,high T2 signal characterized by double-bubble sign on MRI.There were 10 fetuses with jejunoileal atresia,showing bowel dilatation and hyperintense micro-colon on T1WI.Five cases of them depicted expansion of the terminal ileum with high T1 meconium signal.One each fetus had colonic atresia,intestinal malrotation with double-bubble and whirl sign.Annular pancreas with double-bubble sign and pressure trace of the bracket shape was detected in 3 fetuses.Meconium peritonitis was present in 4 fetuses,with 2 of them showing dilatation of intestine,ascites and pseudocysts.Conclusions According to the signal characteristics of amniotic fluid and meconium in the gastrointestinal tract on MRI,the obstructive level and development status of the distal bowel can be determined with MRI.It can provide additional information to ultrasonography,which brings clinical significance to prenatal diagnosis and intrapartum surgical operation.

Objective: To understand the status of occupational health surveillance to workers exposed to toxic environment in a city, so as to provide scientific basis for strategy of occupational diseases prevention. Methods: In January 2017, collecting the data of on-the-job poisonous and harmful workers occupational health surveillance in the city from January 1, 2010 to December 31, 2016 in China Information System for Disease Control and Prevention, the trend of occupational health monitoring in the city was analyzed, and the differences between diverseeconomic types, enterprise scale, industry nature and hazard factors were analyzed and compared. Results: The occupational health examination rate showed an overall upward trend in workers exposed to dust in the city from 2010-2016 (t=3.607, P<0.05) , and the detection rate of occupational contraindications was on the rise in workers exposed to chemical factors (t=3.071, P<0.05). The detection rate of occupational contraindications and suspected occupational diseaseswere significant in different economic types, enterprise scale, industry nature and hazard factors (P<0.05). The detection rate of occupational contraindications was the highest among the large enterprises (1.24%) , the manufacturing industry (0.84%) and the state-owned economy (1.49%). The detection rate of suspected occupational diseases (0.04%) and occupational contraindications (1.15%) were the highest in the physical factors. Conclusion Occupational health monitoring in the city is not optimistic. It is necessary to focuson the occupational health care of manufacturing practitioners, raise the detection rate of suspected occupational diseases, and standard the occupational health check work to protect the occupational health.

Objective: To provide scientific evidence for the prevention and control measures of acute and chronic occupational poisoning by analyzing the epidemiological and occupational characteristics of occupational chemical poisoning cases reported in Tianjin city during 2006-2015. Methods: In September 2016, based on the China Disease Control and Prevention Information System, a database of occupational poisoning cases in Tianjin from 2006 to 2015 was established. The epidemiological characteristics of occupational poisoning were analyzed including three distributions firstly, and then the occupational characteristics were analyzed such as length of service, industry distribution and source of poison. Results: There were 174 occupational poisoning patients in Tianjin from 2006 to 2015, including 16 cases of acute poisoning (9.20%) and 158 cases of chronic poisoning (90.8%) , and the proportion of occupational poisoning cases reported in Binhai New Area is the highest. The number of reported cases of chronic occupational poisoning shows a downward trend. Occupational poisoning cases are concentrated mainly in the age of 40-60 years and length of service of 10-20 years. The high incidence of occupational poisoning mainly exists in the chemical raw materials and chemical manufacturing industry and general, professional equipment manufacturing industry. Acute poisoning was mainly by asphyxial gas (50.00%) , and chronic poisoning was mainly by organic solvent (43.67%) . Conclusion The distribution of occupational poisoning cases in diverse toxic types and regions in Tianjin are quite different. The occupational safety precautions of the chemical raw materials and chemical products manufacturing and occupational poisoning control work in Binhai new area should be strengthened to reduce the occurrence of occupational poisoning.

Objective:To analyze the distribution characteristics of new pneumoconiosis in different industries in Tianjin from 2009 to 2018, in order to provide a theoretical basis for the prevention and treatment of pneumoconiosis.Methods:In November 2019, the data of new pneumoconiosis cases in Tianjin from 2009 to 2018 were collected and classified according to difference industries. The epidemiological characteristics of new pneumoconiosis in different industries were analyzed based on time, type of pneumoconiosis, type of work, age, and working age.Results:A total of 4657 new cases of pneumoconiosis were reported in Tianjin from 2009 to 2018, 4640 cases (99.63%) in the first stage, 13 cases (0.28%) in the second stage, and 4 case (0.09%) in the third stage. The number of new cases increased with time and then decreased. Among them, there were 3482 males (74.77%) and 1175 females (25.23%) . The proportion of women with new pneumoconiosis in the non-metallic mineral products industry was the highest, and the differences were statistically significant ( P<0.05) . There are differences in the distribution of new pneumoconiosis species in different industries (χ 2=4920.11, P<0.05) . Silicosis is mainly distributed in non-metallic mineral products industry, metal smelting and rolling processing industry, petroleum processing coking and nuclear fuel processing industries. Foundry worker′s pneumoconiosis is mainly distributed in the general equipment manufacturing industry and special equipment manufacturing industry, and cement pneumoconiosis is mainly distributed in the non-metallic mineral products industry. The types of work are mainly distributed in smelting, casting, raw materials and forming workers, and the sum of the three accounts for 36.46% (1689/4657) . The M ( P25, P75) of diagnosis age was 56.0 (50.0, 63.0) years, and the M ( P25, P75) of working age was 19.0 (16.0, 26.0) years. The working age of new pneumoconiosis patients in the petroleum processing, coking and nuclear fuel processing industries is 19.0 (16.0, 26.0) years, which is shorter than that of other industries ( P<0.05) ; The diagnostic age of the general equipment manufacturing industry is 54.0 (49.0, 59.0) years, which is less than that of other industries ( P<0.05) . New pneumoconiosis is mainly distributed in large and medium-sized enterprises. New cases of pneumoconiosis in non-metallic mineral products industry, metal smelting, calendar processing industry and general equipment manufacturing industry are mainly distributed in large and medium-sized enterprises. New cases of pneumoconiosis in the metal products industry, special equipment manufacturing industry and petroleum processing, coking and nuclear fuel processing industries are mainly distributed in large enterprises. Conclusion:The work of preventing and controlling pneumoconiosis in Tianjin has a long way to go. We should implement targeted measures according to the characteristics of pneumoconiosis industry, strengthen the supervision of hazard industries, and effectively control the occurrence of pneumoconiosis.

Objective:To analyze the distribution characteristics of new pneumoconiosis in different industries in Tianjin from 2009 to 2018, in order to provide a theoretical basis for the prevention and treatment of pneumoconiosis.Methods:In November 2019, the data of new pneumoconiosis cases in Tianjin from 2009 to 2018 were collected and classified according to difference industries. The epidemiological characteristics of new pneumoconiosis in different industries were analyzed based on time, type of pneumoconiosis, type of work, age, and working age.Results:A total of 4657 new cases of pneumoconiosis were reported in Tianjin from 2009 to 2018, 4640 cases (99.63%) in the first stage, 13 cases (0.28%) in the second stage, and 4 case (0.09%) in the third stage. The number of new cases increased with time and then decreased. Among them, there were 3482 males (74.77%) and 1175 females (25.23%) . The proportion of women with new pneumoconiosis in the non-metallic mineral products industry was the highest, and the differences were statistically significant ( P<0.05) . There are differences in the distribution of new pneumoconiosis species in different industries (χ 2=4920.11, P<0.05) . Silicosis is mainly distributed in non-metallic mineral products industry, metal smelting and rolling processing industry, petroleum processing coking and nuclear fuel processing industries. Foundry worker′s pneumoconiosis is mainly distributed in the general equipment manufacturing industry and special equipment manufacturing industry, and cement pneumoconiosis is mainly distributed in the non-metallic mineral products industry. The types of work are mainly distributed in smelting, casting, raw materials and forming workers, and the sum of the three accounts for 36.46% (1689/4657) . The M ( P25, P75) of diagnosis age was 56.0 (50.0, 63.0) years, and the M ( P25, P75) of working age was 19.0 (16.0, 26.0) years. The working age of new pneumoconiosis patients in the petroleum processing, coking and nuclear fuel processing industries is 19.0 (16.0, 26.0) years, which is shorter than that of other industries ( P<0.05) ; The diagnostic age of the general equipment manufacturing industry is 54.0 (49.0, 59.0) years, which is less than that of other industries ( P<0.05) . New pneumoconiosis is mainly distributed in large and medium-sized enterprises. New cases of pneumoconiosis in non-metallic mineral products industry, metal smelting, calendar processing industry and general equipment manufacturing industry are mainly distributed in large and medium-sized enterprises. New cases of pneumoconiosis in the metal products industry, special equipment manufacturing industry and petroleum processing, coking and nuclear fuel processing industries are mainly distributed in large enterprises. Conclusion:The work of preventing and controlling pneumoconiosis in Tianjin has a long way to go. We should implement targeted measures according to the characteristics of pneumoconiosis industry, strengthen the supervision of hazard industries, and effectively control the occurrence of pneumoconiosis.

Objective: To study the effects p-phenylenediamine (PPD) on lung function and health-related quality of life of occupational exposed workers. Methods: This study was based on data from a company that produce hair dye containing PPD in China. Workers who exposed to PPD were selected as the study group, and workers un-exposed to PPD were selected as the control group. Questionnaires on health-related quality of life of workers using the 36-item Short Form Health Survey (SF-36) . Occupational health examination assessment results were tested in Taizhou Cancer Hospital. The lung function test includes forced vital capacity (FVC) , forced expiratory volume in one second (FEV_1.0) , and ratio of FEV_1.0 to FVC (FEV_1.0/FVC) . Results: The difference in systolic blood pressure between the PPD exposed group and the control group was statistically significant (P<0.05) . FVC, FEV_1.0, and FEV_1.0/FVC of the lung function indexes in the exposed group were lower than those in the control group (P<0.05) . In the health-related quality of life, body pain (P=0.002) , general health (P=0.029) , vitality (P=0.038) , and mental health (P=0.003) were lower in the exposed group than in the control group. Conclusion Occupational exposed to PPD may induce hazard to the workers’lung function and may cause detrimental effect on workers’ health-related quality of life.

Objective: To study the effect of p-phenylenediamine (PPD) on liver and kidney function in occupational exposed workers. Methods: Workers in a hair dye production enterprise which used p-phenylenediamine as a raw material for production were selected as the main research population. Then we conducted a questionnaire survey on the basic conditions of workers and conducted occupational health checkups on general health status, liver and kidney function. Occupational health examination assessment results were tested in Taizhou Cancer Hospital. All data was built using EpiData 3.1 software, and statistical analysis was performed using software SPSS 20.0. Results: The liver function indicators including direct bilirubin, prealbumin, total protein, and white protein, globulin, aspartate aminotransferase, glutamyl transpeptidase, and total bilirubin in the workers exposed to high concentration of PPD were at high normal values, and these indicators were significantly different from low PPD concentration group (P<0.05) . The serum creatinine and serum uric acid in the renal function index were significantly higher in workers exposed to PPD than in workers exposed to low concentrations and in the control group (P<0.05) . Conclusion Occupational exposed to PPD may have a hazard to the workers’ liver and kidney function. Long-term occupational exposure to PPD may lead to increased cumulative exposure of workers, which may cause potential chronic liver and kidney damage in occupationally exposed populations.

OBJECTIVE: To explore the genetic basis of a pedigree affected with Alagille syndrome (ALGS). METHODS: Targeted capture and next generation sequencing was carried out for the proband. Candidate variants were verified by Sanger sequencing among his family members. Their pathogenicity of the variant was predicted with bioinformatic analysis. Clinical characteristics and genotype-phenotype correlation were analyzed. RESULTS: The proband, his elder sister and mother were found to carry a heterozygous c.1270dupG (p.Ala424Glyfs*5) variant of the JAG1 gene, which may lead to premature termination of translation and a truncated protein with loss of function. The variant was unreported previously. The phenotypes of the proband (cholestasis, pulmonary artery stenosis and peculiar faces) have differed from those of his elder sister (cholestasis with pruritus, posterior embryonic ring of cornea) and mother (with no clinical manifestation). Cholestasis and peculiar face of the proband became insignificant with age. CONCLUSION The c.1270dupG (p.Ala424Glyfs*5) variant of the JAG1 gene probably underlay the ALGS in this pedigree with incomplete penetrance.
Aged ; Alagille Syndrome/genetics* ; Heterozygote ; High-Throughput Nucleotide Sequencing ; Humans ; Pedigree ; Phenotype

Objective To investigate the effect of subchronic inhalation of toluene diisocyanate (TDI) on the pathological changes, oxidative stress damage, and HO-1 expression levels in rat liver tissues. Methods Forty healthy 3-week-old SPF-grade Sprague-Dawley male rats were randomly divided into 4 groups (control group, low-dose group, medium-dose group, and high-dose group), each with 10 rats. The rats were placed in a HOPE-MED 8050A movable poison cabinet in a cage. TDI was administered to animals by inhalation at doses of 0, 3.06 mg/m³, 12.25 mg/m³, and 49.00 mg/m³, respectively, for 6 hours a day and 5 days a week, and continuously for 13 weeks. The control group was exposed to fresh air. The effect of TDI on pathological changes, oxidative stress damage and HO-1 expression in rat liver tissues was examined. Results Compared with the control group, the rats in the medium and high-dose TDI-exposed groups exhibited vacuolar changes, hepatocyte swelling, steatosis and other pathological changes. With the increase of the TDI dose, the gap between hepatocytes was widened, mitochondria were swollen and vacuolated, and mitochondrial cristae disappeared. The expression levels of HO-1 gene and protein in the liver tissues of the low, medium, and high dose groups were significantly higher than those in the control group (P<0.05). Compared with the control group, the number of HO-1 positive cells in the low, medium and high dose groups increased and the staining increased gradually, and the difference was statistically significant (P<0.05). Conclusion TDI exposure can cause oxidative damage to rat liver tissues and induce the expression levels of HO-1 gene and protein expression.