The morbidity of hyperuricemia(HUA) has showed an increasing tendency in recent years,along with the improved social living conditions.HUA has been proved to be closely related to kidney diseases,metabolic diseases and cardiovascular diseases.However,people pay little attention to the relationship between uric acid and renal diseases in pediatrics,and the relevant studies and progress are lacking.Now a review is made about the metabolism of uric acid,the etiology and pathogenesis of HUA,and the relationship between HUA and kidney diseases or drugs,in order to pay attention to the relationship between HUA and kidney diseases.

Objective To construct an infectious full-length cDNA clone of enterovirus 71(EV71)and develop a technological platform for study on vaccine development as well as molecular virology of EV71.Methods According to the nucleotide sequence of EV71 strain 085 isolated in China,four pairs of primers were designed for amplification of four end to end overlapping subgenomic cDNA fragments,the cDNA fragments were directional cloned into pBluescript SK(+)vector,and the virus genome cDNA clone was obtained by ligation orderly.The rescued virus of parental strain 085 from RNA transfected host cells was identified by RT-PCR,IFA,titration as well as transmission electron microscope(TEM)after the transcription of the full-length cDNA clone in vitro.Results The full-length cDNA clone was constructed successfully,and the typical CPE was observed after its transcription into Vero cells.The rescued virus with 20-30 nm in diameter can not only be neutralized by EV71 special anti-serum but also react with anti-EV71 monoclonal antibody that virus infected cells stained with FITC can be detected by IFA.After amplification from the total RNA extraction of virus infected cells by RT-PCR with EV71 special primers,the 226 bp products can be detected.The growth curve showed that the rescued virus can propagate in Vero cells stably with a titer of 4.5 ～6.0 lgCCID50/ml during 8 passages.The plaque formed by rescued virus is identical as parental virus in morphology but smaller in size.Conclusion An infectious full-length clone of EV71 was developed successfully,which will be used for further study on pathogenesis and vaccine development of EV71.

The technique of chimeric antigen receptor-engineered T cells (CAR-T) is combined single chain antibody and T-cell activation modification and has specific and durable killing effect on tumors.It had achieved stunning results on clinical trials of B lymphocytic leukemias,B lymphomas and solid tumors.Meanwhile it also had potential risk on the off target effects,cytokine release syndrome,graft versus host disease and so on.This paper briefly reviews the principal of CAR-T cellular mechanism,advantages and clinical applications.

Objective To observe the effect and analyze the advantage of Qilian Fuzheng capsule in maintenance treatment of advanced non-small cell lung cancer. Methods The advanced non-small cell lung cancer patients were randomly divided into observation group and control group, 32 cases in each group. The control group was given the best supportive care, and the observation group was given Qilian Fuzheng capsule 4 capsules three times daily additionally. The tumor control rate, quality of life, PFS and OS of the two groups were compared by follow-up. Results Qilian Fuzheng capsule maintenance treatment of advanced non-small cell lung cancer improved the quality of life. PFS of observation group and control group was 5.4 months and 4.1 months, respectively (P<0.05), and OS of the two groups was 12.3 months and 10.6 months, respectively (P>0.05). The tumor control rate of observation group was 77.42% (24/31), control group was 60.00% (18/30), with no significant difference (P>0.05). Conclusion Qilian Fuzheng capsule is a feasible application in maintenance treatment of advanced non-small cell lung cancer.

Objective To explore the re-treatment approach for nonsmall-cell lung cancer(NSCLC)in clinic with therapeutic alliance of cryotherapy and interventional chemotherapy. Methods Sixty two patients with uncontrolled NSCLC after common radiotherapy and(or)chemotherapy were re-treated with therapeutic alliance of cryotherapy and interventional chemotherapy, and their serum tumor markers of NSE, CEA, CY21-1 and the value(I.S%,I.O.D%)of correlative protein in nucleolus region-Ag-NORs were tested before and after the re-treatment. Meanwhile, patients' one year survival and therapeutic effect to NSCLC were recorded during the follow up. Results After the re-treatment, distinguished decline of the serum tumor markers was observed(P

Objective To discuss the clinical characteristics and the relationship with the survival of smoking lung cancer patients.Methods The clinical data of 368 cases were retrospectively analyzed by comparing the differences of the age,gender,histological type,clinical stage,treatment and tradition Chinese medicine typing,and their correlation with the survival was analyzed.Results Among the 368 patients,the ones aged between 50 to 69 accounted for 69.84％ ; the proportion between the male ones and the female ones was 7.98 ∶ 1 ; the proportion of the squamous cell carcinoma was as high as 52.45％ ; the proportion of the patients with small cell lung cancer in extensive stage was significantly higher than that of the patients in the limited stage(75.38％ ∶ 24.62％) ; the numbers of the patients with non-small cell lung cancer in stage Ⅲ and Ⅳ was significantly higher than that in the stage Ⅰ and Ⅱ (82.77％ ∶ 17.23％) ; the ones with non-surgical treatment accounted for 76.36％,and the proportion of ones with surgery treatment was 23.64％ ; most of the patients were treated by both Chinese medicine and chemotherapy(41.04％) ; the main types of all patients were qi-yin deficiency syndrome and toxic heat flourishing syndrome,accounting for 36.96％ and 29.28％ respectively.The age (F =10.356,P =0.000),clinical stage (F =11.289,P =0.000),treatment methods (t =2.624,P =0.021),smoking index(F =3.681,P =0.021) were the key factors effecting the survival (P ＜ 0.05),and the gender,histological type,tradition Chinese medicine type had no significant effect(P ＞ 0.05).Conclusion The clinical characteristics of the cases are 50-69 aged,male,squamous cell carcinoma,advanced stage,treatment without surgery.The survival of smoking lung cancer patients is effected by the age,clinical stage,treatment methods and smoking index.

[Objective]To observe the expressional variability and correlation of cox-2 and p53 protein between osteosarcoma and osteochondroma and explore their clinical significance.[Method]Sixty Specimens divided into 2 groups were taken from the department of pathology,Third Hospital of Hebei Medical University during 1998~2004.Among them,40(24 male and 16 female)aged 8~38(18.68?6.47)at time of therapy were from cases of osteosarcoma excision;20(13 male and 7 female)aged 9~27(17.95?4.77)from benign lesion of bone(osteochondroma)operation.Immunohistochemistry staining(Streptavidin-Peroxidasc,S-P method)was performed to detect the expressions of cox-2 protein and p53 protein in 40 cases of osteosarcoma.The criteria of positive expression was that brown granule was found in cytoplasm(cox-2)or in nucleus(p53).The percentage of cox-2 or p53 positive expression in five high power fields was cunted randomly in each case.Positive(-):positive oncocytes account 50%.Software SAS v6.12 was used in data analysis.The difference of cox-2 and p53 expressions between osteosarcoma and osteochondroma was analyzed with Chi-square Test.Spearman method was used,with the standard ?=0.05,to analyze the relationship between cox-2 and p53 expression among osteosarcoma.[Result]The positive rate of cox-2 and p53 expressions were 67.5% and 55.0% in osteosarcoma,there was noticeable distinction between osteosarcoma and osteochondroma,most of them was high.There was a positive correlation between them,rs=0.58,P

Epidermal growth factor receptor (EGFR), an important target for tumor targeted therapy, is overexpressed / mutated in the majority of tumors. Immune cells modified by chimeric antigen receptor , including T cells, NK cells and so on, is a new technology in recent years, which has showed an unique advantage in the treatment of malignant tumors. In this paper, the research progress of T cells and NK cells modified with chimeric antigen targeting for EGFR and its mutants will be reviewed.

Objective To systematically evaluate the influence of TNF-α and NSE levels in cerebrospinal fluid on Chinese children with viral encephalitis (VE).Methods Literature about the influence of TNF-α and NSE levels in cerebrospinal fluid on Chinese children with VE,which were published before Nov.1,2016,were searched in databases including CNKI,Wanfang Data,Cochrane Library and PubMed.And the case-control study about the TNF-α and NSE levels in cerebrospinal fluid between Children with VE (VE group) and children without VE (control group) were included in this study,and the quality evaluation was implemented and the data were extracted.And then the RevMan 5.3 was used to carry out the meta-analysis.Results Nine case-control studies were included in this study,involving 536 cases in the VE group and 284 cases in the control group.The results showed that the level of TNF-α and NSE in cerebrospinal fluid of the VE group were all significantly higher than that of the control group,and the differences was statistically significant [SMD=1.75,95%CI:1.56-1.93,P<0.01;SMD=1.34,95%CI:1.17-1.51,P<0.01].Conclusion The levels of TNF-α and NSE in cerebrospinal fluid are positively correlated with the incidence of VE in Chinese children.

BACKGROUND:With the prevalence of total hip arthroplasty,the demand for revision has been increased due to the prosthesis life and various complications.OBJECTIVE:To retrospectively analyze the reasons and clinical effectiveness of revision,clinical application of revision prosthesis,and related revision techniques.METHODS:Clinical data of 85 patients undergoing total hip revision were analyzed retrospectively.Among which,there were 23 cases of biological T.O.P acetabular cup (allograft bone graft) plus polyethylene liner,51 cases of titanium mesh cup plus bone cement fixation,and 11 cases of common acetabular cup;common hip stem in 63 cases (including 47 cases of bone cement fixation);lengthened hip stem in 34 cases (16 cases of modular prosthesis and 18 cases of bone cement fixation).Subsequently,the hip function was assessed by Harris hip scores.RESULTS AND CONCLUSION:All cases were followed up,and the mean follow-up was 30.2 months.(1) The average Harris hip scores changed from preoperative 30.07 to postoperative 90.32.The wound healed well,no dislocation or infection,and the hip function was improved significantly.(2) To conclude,aseptic loosening of the prosthesis is the main cause of reversion occurred,followed by prosthesis dislocation.Acetabular wall bone defect and decrease in bone mass are the main problems in revision.Adequate preoperative preparation for possible and unexpected events,and reasonable selection of prosthesis according to the bone defects will be helpful.Noticeably,early arthroplasty reversion will obtain good clinical efficacy.