This paper reported nine cases of pancreatic lithiasis. Pancreatolithotomy and pancreaticojejunostomy was performed in 4 cases; Pancreatoduodenectomy in 1 case; Laparotomy and biopsy in 1 case complicated with pancreatic carcinoma and hepatic metastasis; And nonoperative treatment in 3 cases. Our data showed that pancreatic fluid stasis caused by stenosis of pancreatic duct and/or the distal segment of bile duct maybe one of the important factors of pancreatic stone formation. The diagnosis and principles of surgical treatment of pancreatic lithiasis were discussed.

Objecctive To study the expression and significance of CD44V6 and E-Cd in gallbladder carcinoma. Methods The expression of CD44V6 and E-Cd was detected by immunohistochemical technique in 48 cases of gallbladder carcinoma, 29 cases of gallbladder adenomal polypi and 25 cases of chronic calculous cholecytitis. The association of CD44V6 and E-Cd expression with the clinical and pathologic parameters was analyzed. Results E-Cd expressed in 21 of 48 cases of gallbladder carcinoma(43 75%), 21 of 29 cases of gallbladder adenomal polypi(72 42%) and 24 of 25 cases of chronic calculous cholecytitis(96 00%), while CD44V6 expressed in 31 of 48 cases of gallbladder carcinoma(64 58%), 9 of 29 cases of gallbladder adenomal polypi(31 03%) and 1 of 25 cases of chronic calculous cholecytitis(4 00%). The expression rates of CD44V6 and E-Cd in the three kinds of tissues were significantly different(P

Objective To detect the effect of exogenous IL-6 on the proliferation of cholangiocarcinoma cell line QBC939.Methods MTT was used to observe the proliferation of cell line QBC939 treated with exogenous IL-6.Following the annexin V/FITC and PI staining,FCM was used to detect the apoptosis of QBC939 treated with exogenous IL-6.Results The proliferation of cell line QBC939 was increased after treatment with IL-6 and was positively related with the concentration of IL-6(P

Objective To study the relationship between the microvesel density(MVD) ,vascular endothelial growth factor(VEGF) , matrix metalloproteinases 2 ( MMP2 ) with the metastasis, prognosis of cholangiocarcinoma. Methods MVD,expression of VEGF and MMP2 were investigated by means of immunohistochemistry in 45 cholangiocarcinoma tissues and 8 normal bile duct tissues. Results (1)MVD and positive expression rates of VEGF , MMP2 in cholangiocarcinoma tissues were significantly higher than those in normal bile duct tissues ( P

Objective To analyze the outcome of patients with acute obstruction of left colorectal cancer treated by modified antegrade colonic lavage with primary tumor resection and anastomosis.Methods From April 2002 to April 2007,112 patients with acute obstruction of left colorectal cancer underwent surgery.During the operation the left colon was exteriorized and placed into a sterilized plastic bag to protect the surgical field from contamination,then a catheter was inserted via the appendix,and after antegrade colonic lavage,primary resection and anastomosis was performed.Results Tumor resection and primary anastomosis was successfully done in the 112 cases.Postoperatively,1 case had anastomotic leak which healed after reoperation with proximal colostomy,and one patient died.Conclusions Modified antegrade colonic lavage is a simple procedure,the bowel can be rapidly decompressed with essentially no contamination,and has a high level of bowel cleansing.It is possible to safely perform primary resection and anastomosis for left colon cancer after the modified antegrade lavage.

Objective To investigate the expressions of phosphatase and tensin homolog deleted on chromosome 10(PTEN) and P53 protein in cholangiocarcinoma and evaluate their possible relationship and clinical significance. Methods The expressions of PTEN and P53 in 36 cases of cholangiocarcinoma, eight cases of normal biliary duct and 30 cases of para-neoplastic tissue were detected by S-P immunohistochemistry method. Results The positive rate of PTEN protein expression was 63.89%(23/36) in cholangiocarcinoma, which was significantly lower than that in normal biliary duct (100%,8/8) and that in para-neoplastic tissue(100%,30/30), and which had no relationship with tumor locus, but was significantly correlated with the degree of differentiation and metastasis. The positive rate of P53 protein expression was 44.44%(16/36) in cholangiocarcinoma, which was significantly higher than that in normal biliary duct( 0%, 0/8) and that in para-neoplastic tissue (0%, 0/30), and it had no relationship with tumor locus and metastasis,but was significantly correlated with the degree of differentiation. The PTEN protein expression had correlation with the expression of P53. Conclusion The expression of PTEN protein was significantly lower in cholangiocarcinoma and significantly correlated with the degree of differentiation and metastasis other than the locus of tumor. The expression of P53 protein was associated with the degree of differentiation, but not with the tumor locus and metastasis. Moreover, The PTEN protein expression had correlation with the P53 expression.

Objective To investigate the correlation of the expressions of PTTG and VEGF proteins in extrahepatic cholangiocarcinoma,and study its role in the development of extrahepatic cholangiocarcinoma.Methods Expression of PTTG and VEGF proteins was detected by SABC immunohistochemical technique in 36 cases of extrahepatic cholangiocarcinoma,30 cases of adjacent histologically noncancerous bile duct tissues and 12 cases of benign bile duct lesions.Results The positive rates of PTTG and VEGF proteins were(72.2)%(26/36) and 83.3%(30/36) respectively,in extrahepatic cholangiocarcinoma;and 63.3%(19/30) and 76.7%(23/30) in adjacent histologically noncancerous bile duct tissues.The expression of PTTG protein was significantly positively correlated with that of VEGF protein(P

Objective To investigate the influence of bile duct ligation ( BDL ) on macrophage ( M ) activity and bacterial translocation in mice . Methods After 7 days of BDL , the levels of serum total bilirubin(TB) , albumin(Alb) , glutamic - oxaloacetic transaminase(AST) , creatinine(Cr) , alkaline phosphatase(ALP) and nitrogen oxide(NO) producted by peritoneal macrophages were measured . The mesenteric lymph nodes were cultured for bacteria . The liver and kidney tissues were histopathologically examined. Results The levels of serum iTB,AST,AKP in BDL group were significantly higher than those in control group(P

Objective To study the significance of expression of VEGF in the hepatocellular carcinoma tissue(HCT) and the expression of AFPmRNA in the peripheral vein blood in hepatocellular carcinoma patients(HCC) with metastasis. Methods AFPmRNA was detected in peripheral blood before operation and the expression of VEGF in HCC tissue was analyzed by immunohistochemistry after operation in 20 patients. The relations between the expression of VEGF protein and AFPmRNA with clinicpathological parameters were analyzed statistically. Results (1)Among the 20 patients with HCC, 10 were positive expression of VEGF protein, which had no relation with the clinicopathological parameters. (2)Of the 20 patients, positive expression of AFPmRNA were detected in the peripheral blood in 15 patients, and AFP≥200?g/L were found in 13 patients.(3) AFPmRNA in the peripheral blood vein was detected in 9 of 10 patients with VEGF positive expression. Conclusions (1)Either VEGF protein expressed in HCC tissue or AFPmRNA detected in the peripheral blood could be as a guide line to judge the tendency of the metastasis of HCC,however former is simpler and the later is more sensitive . (2)The detection of AFPmRNA in the peripheral blood is more sensitive than the detection of serum AFP lever for surveillance of the high risk patients and postoperative follow up of the patients with HCC.

Objective To investigate the relationship between the expression of the Smad4,TGF-?_1 and T?R II and the possible mechanisms in the development of cholangiocarcinoma. Methods Immunohistochemical SABC method was used to examine the expression of Smad4,TGF-?_1 and T?R II in 49 cases of cholangiocarcinoma and 8 nomal common bile duct.Results The expression rates of Smad4,TGF-?_1 and T?R II in cholangiocarcinoma were (40.82)%(20/49),71.43%(35/49) and 36.73%(18/49) respectively; in normal common bile duct tissues were 87.50(7/8),12.50%(1/8) and 100%(8/8) respectively. There was a significant relationship between the (expression) of TGF-?_1 and clinical stage, and tumor metastasis (P