Objective To Construct the prokaryotic expression vector of the fusion gene IFN-?1b/CSPⅡ. MethodsIFN-?1b was amplified from the human genomic DNA by PCR and cloned into prokaryotic expression vector pGEX-4T-1. The recombinant plasmid pGEX-4T-1/IFN-?1b was constructed. Circumsporozoite proteinⅡ(CSPⅡ) was amplified from the Plasmodium falciparum genomic DNA by PCR and was cloned into the prokaryotic expression vector pGEX-4T-1. The recombinant plasmid pGEX-4T-1/CSPⅡ was constructed. IFN-?1b was cut from the recombinant plasmid pGEX-4T-1/IFN-?1b digested with BamHⅠ and EcoRⅠ and ligated with the recombinant plasmid pGEX-4T-1/CSPⅡ also digested with BamHⅠ and EcoRⅠ . The recombinant prokaryotic plasmid pGEX-4T-1/IFN-?1b/CSPⅡ was constructed. The fusion gene IFN-?1b/CSPⅡ was expressed in E.coli by IPTG. Results The prokaryotic expression vector pGEX-4T-1/IFN-?1b, pGEX-4T-1/CSPⅡ and pGEX-4T-1/IFN-?1b/CSPⅡ were identified by PCR, enzyme digestion and gene sequencing. The expressed fusion protein/IFN-?1b/CSPⅡ in E.coli was identified by SDS-PAGE and Western blot. Conclusion The prokaryotic expression vector of the fusion gene IFN-?1b/CSPⅡ was successfully constructed, which was then expressed in E.coli .

Objective To explore the change of plasma D-dimer (DD),lactate dehydrogenase isoenzyme 3 (LDH3),tissue plasminogen activator (t-PA) in the early stage of acute pulmonary embolism and their clinical significance.Methods Forty-six patients with acute pulmonary embolism of early stage (pulmonary embolism group) and 50 cases of normal person (control group) were enrolled in this study.The patients in pulmonary embolism group were divided into low-risk(18 patients),middle-risk(15 patients) and higher-risk (13 patients).The levels of DD,LDH3 and t-PA were detected and compared.Results The levels of DD,LDH3 and t-PA in pulmonary embolism group were (2.95 ± 0.73) mg/L,(421.42 ± 63.29)U/L and (0.85 ±0.02) U/L,and significantly higher than those in control group [(0.03 ±0.01) mg/L,(198.17 ± 23.37) U/L and (0.59 ± 0.02) U/L] (P ＜ 0.05).The levels of DD,LDH3 and t-PA in low-risk patients [(1.49 ± 0.04) mg/L,(315.47 ± 38.24) U/L and (0.63 ± 0.01) U/L] were significantly lower than those in middle-risk patients [(2.22 ±0.27) mg/L,(382.41 ±54.36) U/L and (0.79 ±0.02) U/L] and higher-risk patients [(3.38 ± 0.98) mg/L,(583.93 ± 117.21) U/L and (0.92 ± 0.03) U/L] (P ＜ 0.05).The levels of LDH3 and t-PA in middle-risk patients were significantly lower than those in higher-risk patients (P ＜ 0.05).After treatment,the levels of DD,LDH3 and t-PA were significantly decreased [(2.95 ± 0.73)mg/L vs.(1.03 ±0.11) mg/L,(421.42 ±63.29) U/L vs.(223.24 ±25.04) U/L,(0.85 ±0.02) U/L vs.(0.62 ± 0.02) U/L] (P ＜ 0.05).Conclusions The levels of DD,LDH3 and t-PA in the early stage of acute pulmonary embolism are increased significantly,and with the remission of the patient's condition,the levels of DD,LDH3 and t-PA are decreased.United detection conduces to the diagnosis of acute pulmonary embolism.

Objective To assess the prostaglandin E2 (PGE2) levels in tumor mass and peripheral blood of patients with gastric cancer and explore their clinical significance. Methods The PGE2 levels in tumor mass, tumor margins of excision and peripheral blood of 40 patients with gastric cancer were measured by a kit of PGE2 ELISA. Results The PGE2 levels were higher in the tissues of gastric cancer than in tumor margins of excision [(8.225?2.425) vs (1.669?0.287) ng/g, P

Objective To present the status of smoking control in the Health and Family Planning System in Hubei Province, and to provide scientific basis for smoking control through secret investigations. Methods During the period from July to August in 2015, 168 sanitary Health and Family Planning institutions were chosen by stratified selection to make questionnaires based on Evaluation Standards for Smoke-free Health and Family Planning Institutions issued by the National Health and Family Planning Commission of the People's Republic of China. Secret investigations were conducted in 165 sampled institutions and comparisons were made between smoke-free environment establishment as well as execution processes according to the fraction after converting into centesimal system (actual score/reserved portion × 100). Results The overall score for smoking control in Health and Family Planning System of Hubei Province has climbed to 79.29 ± 12.40.compared to that of 2011 has increased, but 1.6 lower than the national comprehensive evaluated score of 2014. The score of the city (82.14 ± 10.51) proved to be higher than that of the province (66.51±19.69) and the county, (75.35±13.40);disease control institutions (84.77± 9.45) enjoyed a higher score that the others with lowest scores in medical institutions (72.80 ± 14.95);Xianning won the highest score (88.52±5.12);Jingmen (69.81±5.16) and Xiaogan (67.92±7.98) proved to be the lowest in their scores. Nine regions in 17 embraced a lowest discrepancy of over 30 scores and the highest discrepancy of 50.94 scores between the highest score and the lowest among different institutions;12.73% (21/165) medical institutions had no publicity materials; 5/17 municipal institutions and 3/10 county level institutions were still found selling cigarettes, with only 46.06%(76/165) institutions reaching the standard of banning smoking in indoor places, 21.21%(35/165) institutions still have indoor smoking in non-working places, 1.82%(3/165) institutions have indoor smoking in working places. Only 18 (32.14%) institutions out of 56 medical institutions investigated have established smoking cessation clinics. Conclusions Banning-smoking work is less optimistic in general for Health and Family Planning System in Hubei Province, especially for substratum health and family planning institutions. All kinds of departments should take drastic measures to guaranttee the establishment of smoking-free environment.

Objectve To detect whether the CTP(phosphocholine cytidylyltransferase) gene was expressed in the asexual erythrocytic stages of Plasmodium falciparum (FCC 1/HN )by using the RT - PCR and to construct eukaryotic expression vector of CTP. Method The erythrocytic stage parasites of Plasmodium falciparum were cultured as described by Trager and Jensen. RNA from erythrocytic stage parasite was extracted by using Trizol reagent. The complete genes coding for CTP gene isolates FCCI/HN were amplified by reverse transcriptase -polymerase chain reaction(RT- PCR). CTP gene was cloned into eukaryotic expression vector pcDNA3. Results CTP encoding gene was amplified from the erythrocytic stages of Plasmodiumfalciparum (FCC 1/HN) and eukaryotic expression vector of CTP was constructed. Conclusion CTP gene was expressed in the erythrocytic stages of Plasmodium falciparum (FCC 1/HN) and eukaryotic expression vector of CTP was successfully constructed.

Objective To investigate the significance of macrophage migration inhibitory factor(MIF) in ovarian endometriosis (OEms).Methods MIF of ectopic endometriumeutopic endometrium in OEms and endometrium in non-endometriosis (30 cases in every group ) was detected by Elivision TM plus immunohistochemical.Quantitative images analytical system was performed with computer to analyze the mean absorbance(A) of MIF.Results The A of MIF in ectopic and eutopic endometrium of OEms and the normal control was respectively ( 0.180 ± 0.013 ),( 0.158 ± 0.022 ) and ( 0.143 ± 0.029 ),there was statistical significance between groups ( F =47.676,P ＜ 0.001 ).The expression of MIF in proliferative phase of eutopic endometrium of OEms was(0.157 ±0.018),extremely stronger than that in the same phase of the normal control (0.146 ± 0.029 ) ( t =2.656,P =0.009 ) ; and MIF in secretory phase of eutopic endometrium of OEms was (0.160±0.028),obviously higher than that in the same phase of the normal control(0.137 ±0.030) (t =3.059,P =0.003).Conclusion The MIF may play a significant role in the pathogenesis and development of ovarian endometriosis.

Objective To investigate the significance of hypoxia inducible factor-1(HIF-1) expression in ovarian endometriosis (Oems). Methods Elivision Tm plus Immunohistochemical and Stereology methods were used to detect HIF-1 expression in 30 cases of ectopic endometrium, 30 cases of eutopic endometrium in ovarian endometriosis and 30 cases of endometrium in ovarian teratoma as the normal control. Quantitative images analysis was performed with the aid of computer to collect and analyze the mean optical density (MOD) of HIF-1 expression. Results The MOD values of HIF-1 in ectopic and eutopic endometrium of Oems and the normal control were 0. 077 ± 0. 014, 0. 070 ± 0. 013 and 0. 061 ± 0. 007 respectively, which showed significantly differences between each other groups(F = 37. 738, P ＜ 0. 01 ＝. HIF-1 expression in proliferative phase (0.076 ±0.007) of eutopic endometrium of Oems was significantly higher than that in secretary phase (0. 059 ±0. 014) (t = 6. 918 ,P ＜0. 01 ＝; which was also significantly higher than that of the normal control in the same phase (0.060±0.007) (t=-12.724,P ＜0.01 ＝.Conclusion HIF-1 may play an important role in the pathogenesis and development of Oems.

0.05),but the total expenses and antibiotic cost of the medical treatment in group B were obviously lower than those in group A(P

OBJECTIVE To investigate the ESBLs ′ genotypes and the resistance of Escherichia coli and Klebsiella pneumoniae in Taizhou Hospital of Zhejiang Province.METHODS We collected 30 strains of E.coli and K.pneumoniae expressing ESBLs,then analyzed their encoding genotypes of TEM,SHV,PER,VEB and CTX genes by PCR and DNA sequencing technology separately.RESULTS There were 26 strains expressing blaCTS-M gene among 30 strains,in which 16 strains belonged to blaCTS-M-9 subgroup genotype,8 strains to blaCTX-M-1,7 strains to blaCTX-M-2,4 strains to blaCTX-M-1 as well as blaCTX-M-2,1 strain to blaCTX-M-2 as well as blaCTX-M-9 and 1 strain belonged to blaCTX-M-1 as well as blaCTX-M-9.CONCLUSIONS The prevalent clinical genotype of E.coli and K.pneumoniae is blaCTX-M-9.

OBJECTIVE:To discuss the medication regularity of Chinese herbal compound formula for osteoporosis. METHODS:A total of 104 Chinese herbal compound formula for osteoporosis (OP) published from 1995 to 2005 were retrieved from China Hospital Knowledge Database(CHKD); and a database was set up using EXCEL2000 software,with the drug name of each herbal medicine in the compound herbal formula included and a statistical analysis was carried out.The herbs which were used frequently were subjected to data mining and analysis using association rules. RESULTS:Of the total 104 Chinese herbal compound formula for osteoporosis,leading the first 12 places in terms of application frequency in turn were prepared Rehmannia glutinosa,Herba Epimedii,Dioscorea opposita and Eucommia ulmoides,etc.In the association rule set,there were respectively 7,6,6 and 8 rules which respectively consisted of 2,3,4 and(5,6,7) herbs.CONCLUSION:The relationship between the three factors(renal deficiency,splenic asthenia,and blood deficiency) and osteoporosis were verified in the study of the Chinese herbal compound formula for osteoporosis.