OBJECTIVETo establish an HPLC method for simultaneous determination of eight iridiods in Gardeniae Fructus.

METHODKromasil C18 column (4. 6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-water-trifluoroacetic acid (6:94: 0.05) as the mobile phase at the flow rate of 1.0 mL x min(-1). The detection wavelength was set at 238 nm, and the column temperature was 40 degrees C.

RESULTThe linear ranges of geniposide, gardoside, shanzhiside, geniposidic acid, deacetyl asperulosidic acid methyl ester, gardenoside, scandoside methyl ester, and genipin gentiobioside were 1.5036 - 15.036, 0.04256 - 0.4256, 0.1038 - 1.038, 0.00992 - 0.0992, 0.02332 - 0.2332, 0.4128 - 4.128, 0.02040 - 0.2040 and 0.4656 - 4.656 microg, respectively. Their average recoveries were 99.6% , 100.6% , 101.2%, 99.5%, 100.3% , 98.7%, 99.8% and 100.1%, respectively.

CONCLUSIONThe method shows good separation and it is so simple, accurate and highly repeatable that it can be used for providing basis for quality control of Gardeniae Fructus.

Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Gardenia ; chemistry ; Iridoid Glycosides ; analysis ; isolation & purification

Objective To study influence of hospital-community-family integrated nursing model for patients with coronary heart disease based on Omaha system under the " Nightingale + " era.Methods A total of 90 coronary heart disease patients were selected as research objects,and were divided into observation group and control group according to digital table method,with 45 cases per group.The control group implemented traditional nursing,while observation group implemented hospital-community-family integrated nursing model based on Omaha system under the " Nightingale +" era.Results The observation group had higher compliance,quality of life after discharge than the control group (P ＜ 0.05).The incidence rates of primary and secondary cardiovascular adverse events were lower than that in the control group (P ＜ 0.05).Conclusion Hospital-community-family integrated nursing model for patients with coronary heart disease based on Omaha system is feasible,and can enhance compliance after discharge coronary heart disease patients,improve the quality of life,reduce the incidence rate of cardiovascular adverse events.

Objective To study influence of hospital-community-family integrated nursing model for patients with coronary heart disease based on Omaha system under the " Nightingale + " era.Methods A total of 90 coronary heart disease patients were selected as research objects,and were divided into observation group and control group according to digital table method,with 45 cases per group.The control group implemented traditional nursing,while observation group implemented hospital-community-family integrated nursing model based on Omaha system under the " Nightingale +" era.Results The observation group had higher compliance,quality of life after discharge than the control group (P ＜ 0.05).The incidence rates of primary and secondary cardiovascular adverse events were lower than that in the control group (P ＜ 0.05).Conclusion Hospital-community-family integrated nursing model for patients with coronary heart disease based on Omaha system is feasible,and can enhance compliance after discharge coronary heart disease patients,improve the quality of life,reduce the incidence rate of cardiovascular adverse events.