1.CT and MRI findings of ovarian granulosa cell tumor
Journal of Practical Radiology 2017;33(9):1397-1400
Objective To study the CT and MRI features of ovarian granulosa cell tumor(OGCT).Methods The clinical and imaging data of 12 cases with OGCT proved by pathology were analyzed retrospectively(10 cases were performed with CT,5 cases were performed with MRI,and 3 cases were performed with both CT and MRI).Results All lesions were solitary.The tumors presented round,oval, or lobulated with clear boundary.In 12 cases, 3 cases were multiple cystic masses with uneven thickness of cyst walls and septation.Mild enhancement of the cyst walls and septation was showed after contrast enhancement.7 cases were cystic and solid masses with multiple cysts, typically with sponge-like pattern,and most cases showed slightly to moderately enhancement.2 cases were solid mass with septation,1 case showed significant enhancement,and 1 case showed slightly enhancement.6 of 12 cases presented enlargement of the utreus and endometrium thickening,1 case was associated with endometrial carcinoma.Conclusion OGCT has characteristic CT and MRI features, and CT and MRI examinations combined with estrogen abnormality are helpful for diagnosis.
2.Correlation analysis of type A influenza virus genetic variation characteristic with survival selective pressure
Xiaoming ZHOU ; Huifang ZHAO ; Jialiang LU
Chinese Journal of Infectious Diseases 1997;0(04):-
Objective Study the relationship between type A influenza virus genetic variation with survival selective pressure, help for the finding of possible vaccine conserved antigen target. Methods Select seven strains of same HA (Hemagglutinin) serotype, regional and isolation time closely related type A influenza virus with full HA gene coding sequence; use Blast2 program to calculate the parameter of nucleotide conservative, amino acid conservative, mutation ratio of codon 3rd (over) non 3rd locus, survival selective pressure indicator of these virus strains; analysis the parameters relationship with survival selective pressure indicator. Results Nucleotide conservative of HA gene is significantly lower than that of other genes; amino acid conservative of HA gene is similar with NS gene, all lower than that of other genes; genetic variation of HA gene is under survival selective pressure; selective pressure toward NS, PB1 gene is relatively lower than that toward NA, NP gene; survival selective pressure indicator is strongly correlated with mutation frequency upon codon 3rd locus. Conclusions Genetic variation of influenza virus is determined both by mechanism of relax replication model and survival selective pressure; genetic conservative of each gene is different, NA, NP gene could be selected as possible vaccine target for their relative high conservative, HA gene possesses medium genetic conservative with prospective of finding more conserved epitope region within its full sequence, NS, PB1 are not recommended as vaccine candidate for their relative low genetic conservative.
3.Protective effect of phospholipase A_2 inhibitor on vital organs injury from intestinal ischemia-reperfusion
Gang ZHAO ; Huifang XU ; Wei JIANG
Chinese Journal of Anesthesiology 1996;0(09):-
Phospholipase A_2 (PLA_2) inhibitor quinacrine was used to explore protective effect on multiple organ dysfunction (MOD) caused by intestinal ischemia-reperfusion (I/R) in rats. Gut I/R caused the increase of gut PLA_2 activity and induced endotoxemia and bacteriemia. Pretreatment with intravenous quinacrine 10mg?kg~(-1) attenuated bacteria and endotoxin translocation,markedly lowered the levels of thromboxane A_2 and prostacyclin I_2 in blood,and provided protection from the development of vital organs dysfunction. As a result,the survival rate in pretreatment group increased by 25%. The results demonstrate that gut I/R promotes gut barrier failure,then contributes to the development of MOD by allowing bacteria or endotoxin reaching the circulation. PLA_2 and PLA_2-dependent lipid mediators play an important role in the development of gut I/R injury and MOD. Intravenous quinacrine has protection against MOD resulting from gut I/R.
4.A comparison between laparoscopic and open intrafascial hysterectomy
Yinghua ZHAO ; Huifang CHEN ; Jieyan LI
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To evaluate the clinical significancy of laparoscopic intrafascial hysterectomy. Methods We reviewed 37 cases of laparoscopic intrafascial hysterectomy and 45 cases of open intrafascial hysterectomy. A controlled study on operative time, blood loss, time to first flatus, postoperative morbidity and complications was made. Results All operations in two groups were successfully completed, and no severe complications were seen. In laparoscopic group and open group, the operative time, blood loss, time to first flatus, and postoperative morbidity were (158?36.7) min and (99?24.3) min (t=8.714, P
5.A 10 years retrospective study of the projects supported by the ministry of national science and technology in oral medicine
Yijiao ZHAO ; Huifang YANG ; Yong WANG
Chinese Journal of Medical Science Research Management 2016;29(3):192-195,205
Objective To investigate the projects funded by ministry of national science and technology (NSTP) in China allocated to oral medicine from 2003 to 2013.Methods The data regarding the NSTP allocated to oral medicine research from 2003 to 2013 were collected,including funding years,the expenses and numbers of programs,the secondary discipline of oral medicine and the program units,etc.Multidimensional analyses were provided subsequently.Results From 2003 to 2013,a total number of 59 projects and 210 million CNY supported by NSTP were allocated to oral medicineresearch.The development trend showed increased year by year.The oral medicine researches were allocated by almost all major types of NSTP and the funding directions were tending to diversified development.But,the projects units were relatively concentrated in clinical,teaching and research institutions,only few enterprises.Conclusions Over the past 10 years,the sustainable improvement of NSTP in oral medicine research has led to the development of oral medicine discipline.NSTP has becoming an important way for oral medicine research to obtain State-level funding.
6.Experimental study on effect of pulse-actlvating injection on injury of free radical in acute paraquat poison-ing in rats
Huifang LIU ; Yanyan ZHAO ; Chunsheng CHEN
Clinical Medicine of China 2009;25(10):1035-1037
Objective To study the therapeutic effect of pulse-activating injection on acute poisoning by pa-raquat(PQ). Methods 50 Sprague-Dawley (SD) rats were randomly divided into five experimental groups: blank group, negative control group, positive control group, low-dose pulse-activating injection group (LDG), and high-dose pulse-activating injection group(HDG) (n = 10 for each group). Blank group were injected with normal suline,30 ml/kg,and other groups were established as acute paraquat poisoning models. Macroscopic and histopathological ex-aminations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) both in plasma and bronchoalveolar lavage flui(BALF) were detected. Results Compared with that in negative control group,lung congestion and lung edema of LDG group were mitigated; and the MDA level decreased from (5.04± 0.50) nmol/ml,(1. 19±0.18) nmol/ml to (4.04±0.21) nmol/ml,(0.79±0.04) nmol/ml both in plasma and BALF;the SOD activities were increased from (123.30±20. 39) U/ml, (26.43±2.22) U/ml to (277.09± 11.66) U/ml,(37.10± 2.49) U/ml as well; the GSH-Px activities were increased from (1796.63 ±81. 12) U/ml, (598.24 ± 62.50) U/ml to (2151.54 ± 148.32) U/ml, ( 1788.44 ± 175.11 ) U/ml as well ( P < 0. 01 ). Conclusions Administration of pulse-activating injection could improve the lipid peroxidation damage caused by a-cute poisoning of PQ.
7.Relationship between subjective well-being,general self-efficacy,working efficiency and psychological status of nurses
Huifang HE ; Qingguo ZHAO ; Juan FENG
Chinese Journal of Behavioral Medicine and Brain Science 2009;18(4):338-339
Objective To study the relationship between general self-efficacy, social support, psychological health and working efficiency.Methods Using General Self-efficacy Scale (GSES), Social Support Rating Scale, Symptom Checklist-90 (SCL-90) and supervisor's rating of work efficiency to investigate 262 nurses. And using correlation and regression analyze the results. Results Correlation analysis found that general self-efficacy and subjective social support had significant negative correlations with the general symptomatic index of SCL-90( r =-0.31,-0.29,P <0.05).Results of regression analysis showed that general self-efficacy, objective social support, using of the social support, and general symptomatic index of SCL-90 had significant effects on working efficiency (β=0.41,0.36,0.35,-0.28,P <0.05).Conclusion Working efficiency of nurses is mainly effected by general self-efficacy, objective social support, using of the social support and psychological status. As a result,all these factors should be taken under consideration in order to improve working efficiencies of nurses.
8.Surgical treatment for correcting abnormal mixed head position in nystagmus with a predominant head turn
Jing ZHAO ; Huifang HAN ; Weifeng SUN
Ophthalmology in China 2009;18(4):275-278
Objective To evaluate the surgical effect for congenital nystagmus (CN) with an abnormal head position in all three axes of a predominant head turn. Design Retrospective case series. Participants 11 patients with horizontal nystagmus and abnormal head position treated in Xingtai Eye Hospital during 2003-2008. All had an abnormal head position in all three axes with a predomi-nant head turn. Methods Recession of the yoke muscles responsible for the slow phase of nystagmus (one medial rectus of 6mm, and one lateral rectus 8 mm), and simultaneously resection of the yoke muscles responsible for the fast phase of nystagmus (the other lateral rectus 9 mm, and medial rectus 7 mm) were performed. The follow-up was from 6 to 20 months. Main Outcome Measures The visual acuity of both eyes in primary position, the degree of head turn, chin elevation or depression, head tilt. Results At the last follow-up, the all 11 patients undergoing the operation for CN gained two or more lines of Snellen acuity with both eyes in primary position for be-ing corrected best. The postoperative mixed head position of 9 cases got disappear or ≤ 10°. Of 1 case developed overcorrection of the head turn. 1 case has the head turn of ≤15°, and 1 case remained the head turn of 25°. Conclusion Recessions and resections of the horizontal yoke rectus muscles in nystagmus with blockage position, when the head turn predominates over the vertical and torsional components, are effective in diminishing the abnormal head position on all three axes, and improve the visual acuity with both eyes in primary position. (Ophthalmol CHN, 2009, 18: 275-278)
9.Determination of Paraquat in Serum by Ultraviolet Spectrophotometry
Yanyan ZHAO ; Huifang LIU ; Lina HAO
Journal of Environment and Health 1992;0(05):-
Objective To establish a method for determining paraquat in the serum by ultraviolet spectrophotometry and present a quantitative index for paraquat poisoning salvage. Methods The sample was deproteinizated by 20% trichloracetic acid(TAC). 50 ?l microcell was used, the detection wavelength was 257 nm. Results The linearity was within 0.05~50 ?g/ml, r=0.999 9. The average recovery rates were 90.0%-102.4% and RSD=3.9%(n=4), the lowest detection limit was 0.01 ?g/ml. Conclusion The method is simple, rapid, precise and suitable for the determination of paraquat in the serum.
10.Efficacy of imatinib plus granulocyte-colony-stimulating factor for treatment of patients with chronic myeloid leukemia
Huifang ZHAO ; Yongping SONG ; Baijun FANG ; Ning LI ; Xudong WEI
Journal of Leukemia & Lymphoma 2011;20(2):92-94
Objective To study the treatment effect by addition of granulocyte-colony-stimulating factor (G-CSF) that could reduce the level of residual disease in patients with Ph-positive chronic myeloid leukemia (CML) who appeared to have achieved a suboptimal response to imatinib (IM) alone. Methods Eleven patients with CML who had achieved≥ 35 % Ph-negativity on treatment of IM were enrolled. The initial dose of imatinib was 400 mg or 600 mg orally daily, and G-CSF, 5 μg/kg subcutaneously daily. The administration of G-CSF was postponed or interrupted in the event of leukocytosis (leukocytes ≥ 30 ×109/L) until the whitecell count fell <20 × 109/L. Efficacy was assessed by serial monitoring of blood levels of bcr-abl transcripts.Treatment with G-CSF was discontinued if the patient did not achieve a reduction in the transcript level of at least 0.5 log after 6 months. For patients whose bcr-abl transcript levels continued to decline but had not yet reached molecular remission, treatment was designed to continue for 1 to 6 months. Results Of 11 evaluable patients, nine had an appreciable decline in bcr-abl transcript levels(include 7 cases the reduction was greater than 1 log and 2 cases the reduction was greater than 0.5 log), 2 cases the reduction was lower than 0.5 log.In 7 cases the reduction was greater than 1 log, including five patients who did not achieved complete cytogenetic response and two patients achieved complete molecular responses. No bleeding episodes occurred.No patient discontinued therapy because of toxicity and there were no treatment-related deaths. Conclusion The addition of G-CSF should be considered safely and successfully for patients who fail to obtain optimal response to IM alone and this approach deserves further evaluation.