Objective To investigate the projects funded by ministry of national science and technology (NSTP) in China allocated to oral medicine from 2003 to 2013.Methods The data regarding the NSTP allocated to oral medicine research from 2003 to 2013 were collected,including funding years,the expenses and numbers of programs,the secondary discipline of oral medicine and the program units,etc.Multidimensional analyses were provided subsequently.Results From 2003 to 2013,a total number of 59 projects and 210 million CNY supported by NSTP were allocated to oral medicineresearch.The development trend showed increased year by year.The oral medicine researches were allocated by almost all major types of NSTP and the funding directions were tending to diversified development.But,the projects units were relatively concentrated in clinical,teaching and research institutions,only few enterprises.Conclusions Over the past 10 years,the sustainable improvement of NSTP in oral medicine research has led to the development of oral medicine discipline.NSTP has becoming an important way for oral medicine research to obtain State-level funding.

AIM: To explore the existence of deficiency of TGF-?_1 in leukemia cells and its possible mechanism in the pathogenesis of leukemia. METHODS: The levels of TGF-?_1 were detected by ELISA in the cultured supernatant of leukemia cell lines and primary cells from patients with acute leukemia. TGF-?_1 gene was transduced into HL-60 cells by lipofectin-mediated DNA transfection. In the presence of G418, the HL-60 clone expressing TGF-?_1 was selected. The effects of exogenous TGF-?_1 gene on the proliferation and apoptosis of HL-60 cells were studied by leukemic colony assay, tumorigenicity in athymic nude mice, DNA fragmentation and cell cycle analysis. The expression of intrinsic TGF-?_1, [STBX]bcl-2 oncogene, hTERT mRNA on the apoptosis of HL-60 cells induced by exogenous TGF-?_1 gene were detected by RT-PCR. RESULTS: The levels of TGF-?_1 were obviously lower in the supernatant of leukemia cell lines and primary cells from patients with acute leukemia, as compared with normal controls (P

Objective:To compare two digital methods of quantitatively accessing the degree of facial asymmetry by three-dimensional data.Methods: The three-dimensional data of 20 subjects were got by the FaceScan, and then were input to the reverse engineering software Imageware 13.0 and Geomagic 12 .Their mirror data were acquired and superimposed with the original data by the methods of interactive closest points ( ICP) and Procrustes analysis ( PA) .The mid-sagittal planes of the two methods were ex-tracted respectively, the degree of facial asymmetry and the distance of 21 automatic landmarks to mid-sagittal plane were calculated and compared.Results:The paired t test was taken and t=1.346, P=0.193.Conclusion:We can safely come to the conclusions that for the subjects with no evident facial asymmetry, there are no significant difference between the PA and the ICP methods for extracting the mid-sagittal plane from three-dimensional data.

Objective To explore the MSCT manifestations of pulmonary focal ground-glass opacity (GGO)nodules with differ-ent pathological types.Methods The thin-layer CT images of 87 patients with GGO nodules were retrospectively reviewed.Of them, 57 cases were confirmed by surgery and pathology,including 8 inflammations,2 benign tumors,1 1 adenomatous hyperplasias (AAH),1 9 carcinomas in situ (AIS),and 1 7 adenocarcinomas.The other 30 cases were followed up without surgery.Results Blur edge was found in 6 of the 8 inflammations (75.0%).However,the organized pneumonias showed large irregular shape with clear edge.Of 30 pre-infiltrating lesions (AAH or AIS),28 (93.3%)were ranged from 0.8 cm to 2.0 cm,26 (86.7%)were round or nearly round,25 (83.8%)showed clear edge,and 25 (83.8%)had blood vessels penetrated signs.Of 17 adenocarcinomas,15 (88.2%) were larger than 2.0 cm,13 (76.5%)showed irregular or lobular shape,8 (47.0%)showed coarse edge,and 10 (58.8%)had pleural indentation signs.Of 36 AISs and adenocarcinomas,9 were solid components (specificity of 95.2%),5 had vacuole signs (specificity of 95.2%),and 13 had penetrated and enlarged blood vessels (specificity of 100%).Conclusion There are characteristic MSCT appearances for different pathological types of GGOs.

Objective To select the risk factors and focus on the pathogenesis of anterior ischemic optic neuropathy (AION) after cataract surgery.Methods A retrospective review of all patients with phacoemulsification surgery referred to the Ophthalmology Divisions,the First Affiliated Hospital,Zhengzhou University,from September 1,2010 to September 1,2016 was performed.Eligible patients were 11 206 cases(13 320 eyes),30 cases (30 eyes) were complicated with AION after cataract surgery (AION group),and 90 cases (90 eyes) were selected as control group according to the ratio of 1 ∶ 3 by random sequence.Factors of small cup disc ratios,previous surgery history,cardiac disease,diabetes,hypertension,hyperlipemia,smoking,carotid disease and intraocular pressure (IOP) were collected,x2 test,Logistic regression and t test were performed to analyze risk factors for AION.Results Small cup-disc ratios,diabetes,hypertension,hyperlipemia,carotid disease were influencing factors of AION after cataract surgery.Hyperlipemia and carotid disease were risk factors of AION after cataract surgery.There was no significant difference in preoperative intraocular pressure between two groups(all P ＞ 0.05).The intraocular pressure at postoperative 1 day and 7 days in AION group were higher than those in control group (all P ＜ 0.05).Conclusion Hyperlipemia and carotid artery disease are risk factors for AION after cataract surgery,and high intraocular pressure may be the inductive factor of AION.

Objective:To evaluate the actual measurement accuracy of 2 three-dimensional(3D)facial scanners for real person. Methods:3D digital face models of 1 0 volunteers with normal ficial form were obtained by 3dMD and FaceScan facial scanners respec-tively.The measurement values of 1 0 feature lengths and 5 feature angles were measured on each 3D model by the software respective-ly.The reference values of all characteristics were acquired by line laser scanner (Faro)with high accuracy.Statistical and surveying analysis were taken between the measurement values and reference values.Facial morphology measurement error and actual accuracy of facial scanners were obtained finally.Data were statistically analysed.Results:The length measurement accuracy of 3dMD and FaceS-can was(-0.37 ±0.68)mm and (-0.29 ±0.53)mm(P =0.223),the angle measurement accuracy was (-0.22 ±2.1 4)°and (0.1 2 ±2.69)°(P =0.428),respectively.Conclusion:The 3D data of ficial morphology obtained by the 2 scanners are not signifi-cantly different.

Objective:To compare the accuracyof interactive closet point (ICP)algorithm,Procrustes analysis (PA)algorithm,andalandmark-independent method to construct the mid-sagittal plane (MSP)of the cone beam computed tomography.To provide theoretical basis for establishing coordinate systemof CBCT images and symmetric analysis.Methods:Ten patients were selected and scanned by CBCT before orthodontic treatment.The scan data was imported into Mimics 10.0 to reconstructthree dimensional skulls.And the MSP of each skull was generated by ICP algorithm,PA algorithm and landmark-independ-ent method.MSP extracted by ICP algorithm or PA algorithm involvedthree steps.First,the 3D skull processing was performed by reverse engineering software geomagic studio 2012 to obtain the mirror skull. Then,the original and its mirror skull was registered separately by ICP algorithm in geomagic studio 2012 and PA algorithm in NX Imageware 1 1 .0.Finally,the registered data were united into new data to calcu-late the MSP of the originaldata in geomagic studio 2012.The mid-sagittal plane was determined by SEL-LA (S),nasion (N),basion (Ba)as traditional landmark-dependent methodconducted in software InVivoDental 5.0.The distance from 9 pairs of symmetric anatomical marked points to three sagittal plane were measured and calculated to compare the differences of the absolute value.The one-way ANOVA test was used to analyze the variable differences among the 3 MSPs.The pairwise comparison was performed with LSD method.Results:MSPs calculated by the three methods were available for clinic analysis,which could be concluded from the front view.However,there was significant differences among the distances from the 9 pairs of symmetric anatomical marked points to the MSPs (F=10.932,P=0.001).LSD test showed there was no significant difference between the ICP algorithm and landmark-independent method (P=0.1 1 ),while there was significant difference between the PA algorithm and landmark-independent methods (P =0.01 ).Conclusion:Mid-sagittal plane of 3D skulls could be generated base on ICP algorithm or PA algorithm.There was no significant difference between the ICP algorithm and landmark-independent method.For the subjects with no evident asymmetry,ICP algorithm is feasible in clinical analysis.

Objective To explore the influence of alimentary tract reconstruction after gastrectomy on the blood glucose level in patients with gastric cancers combined with type 2 diabetes mellitus. Methods From January 2004 to December 2009, the level of blood glucose and body weight before operation and 1,3,6 months after operation in 87 gastric cancer combined with type 2 diabetes mellims patients were retrospectively analyzed. These patients underwent different alimentary tract reconstructions,including 48 patients for Billroth I after distal subtotal gastrectomy (group A), 39 patients for esophageal Roux-en-Y jejunostomy after total gastrectomy (group B). Fasting blood glucose (FBG) level and body weight of these patients were compared. Results In group A, change of FBG before and after operation were not significant (P ＞ 0.05 ). The levels of FBG in group B were significantly lower in 1,3,6 months after operation [(6.7 ±0.8), (6.6 ±0.6), (6.8 ±0.7) mmol/L] than that before operation [(9.7 ± 1.4) mmol/L](P＜0.05). The lower value average difference of FBG at 1,3,6 months was significant between group A and group B (P＜0.05 ). In group B, 6 months after operation's total effective rate was 87.2% (34/39). Changes of body weight before and after operation in group A and group B were significant (P ＜ 0.05 ). But between two groups, the changes of body weight between 1,3,6 months and before operation were not significant (P ＞0.05).Conclusions Esophageal Roux-en-Y jejunostomy after total gastrectomy has obvious influence on FBG level in patients with gastric cancers combined with type 2 diabetes mellitus. It takes about 1 month to reveal the effect of operation and has nothing to do with weight loss.

Objective To explore the normal values for two-dimension solid state high resolution anorectal manometry (HRAM) in healthy adult volunteers.Methods The healthy adult volunteers were recruited by advertisement and underwent solid state HRAM in the left lateral position.Anorectal pressures and rectal sensation were recorded and analyzed.Results (1) A total of 126 Chinese healthy adult volunteers (male:50 cases (39.7％);age:(37.5 ± 14.2) years old) were recruited in this study.(2)Mean anal resting pressure (MERP) was (71.8 ± 17.3) mmHg (1 mmHg =0.133 kPa).Maximum anal resting pressure (MARP) was (79.3 ± 17.8) mmHg,Maximum anal squeeze pressure (MSP) was (178.7 ± 52.8) mmHg.Anal high pressure zone (HPZ) length was (3.4 ± 0.6) cm.During simulated evacuation,residual anal pressure (RAP) was (63.8 ±20.5) mmHg,and anal relaxation rate (ARR) was (37.0 ± 11.5) ％.Rectal threshold volume for first sensation (FST),desire to defecate (DDT),urgency to defecate (UDT) and maximum discomfort (MDT) was (47.4 ±10.0) ml,(84.5 ±18.2) ml,(125.8 ± 28.5) ml,and (175.5 ±36.1) ml,respectively.(3) Compared with female subjects,male subjects had higher MSP [(211.0 ± 50.7) mmHg vs (157.5 ± 42.5) mmHg],RAP [(71.6 ± 18.1) mmHg vs (58.8 ± 20.5) mmHg]and rectal MDT[(187.0 ±36.4) mmHg vs (168.0 ±34.1)mmHg],but lower ARR [(32.1 ±8.0)％ vs (40.2 ±12.3)％],all P＜0.01.(4) MERP,MARP,MSP and rectal MDT were higher in young group (≤ 40 years old),all P ＜ 0.05.Conclusions These observations provide normal values for two-dimension solid state HRAM,which have significant difference between genders and different age groups.

Objective: To investigate the value of longitudinal strain and peak strain dispersion in the evaluation of left ventricular longitudinal strain and longitudinal strain synchrony in essential hypertensive patients with preserved ejection fraction. Methods: A total of 50 essential hypertensive patients with preserved ejection fraction were collected as hypertensive group, they were divided into left ventricular hypertrophy( LVH) group (n=29) and non left ventricular hypertrophy(NLVH) group(n=21), and 26 healthy volunteers were collected as control group, two-dimensional echocardiography was performed. Peak systolic longitudinal strain(LPS), global LPS (GLPS), time to LPS (TTPLS) and longitudinal peak strain dispersion (PSD) were determined by the offline EchoPAC 201.54 software. It can automatically obtaine the bull′s-eye map of the corresponding 18-segmental LPS and TTPLS. Results: Left atrial diameter (LAD) was higher in NLVH group than that in control group(P<0.05). LAD, left ventricle end-systolic diameter(LVESD), left ventricular end diastolic diameter(LVEDD), interventricular septal thickness (IVST), left ventricular mass index (LVMI) and left ventricular posterior wall thickness (LVPWT) were higher in the LVH group than those in the control group and NLVH group (all P<0.05), there was no significant differences in left ventricular ejection fraction(LVEF) among the three groups (all P>0.05). LPS and TTPLS: LPS of basal, middle, apical left ventricular segments were significantly lower and TTPLS of middle left ventricular segments were significantly longer in the LVH group than those in the control group and NLVH group (all P<0.05). LPS of middle left ventricular segments was significantly lower in the NLVH group and TTPLS of basal left ventricular segments was obviously greater in the LVH group than those in the control group (all P<0.05). PSD increased and absolute value of GLPS decreased gradually in the control group, NLVH group and LVH group(all P<0.05). There was a negative correlation between absolute value of GLPS and PSD in the hypertensive group (r=-0.61, P<0.01). Measurement of PSD had well repeatability and stability. Conclusions Longitudinal strain and PSD are promising approach to evaluate left ventricular longitudinal systolic function earlier in hypertensive patients with preserved ejection fraction. It has well repeatability and stability and it provides help for clinical diagnosis and efficacy assessment.