Objective To analyze the diagnostic value of human leukocyte antigen(HLA)-B27 expression for ankylosing spondylitis.Methods 100 patients with ankylosing spondylitis were selected as the study group. 100 healthy people were selected as the control group.HLA -B27 beads enzyme immunoassay reagent kits was used to implement qualitative detection of fresh whole blood in the study group,and compared HLA -B27 positive rates of the two groups,as well as the diagnosis value of HLA -B27 antigen expression.Results The HLA -B27 positive rate of the study group was 73.00%,which was higher than 9.00% of the control group,the difference was statistically significant (χ2 =84.66,P <0.01).The diagnosis rate of the male patients was 90.91%,which was higher than 72.41% of the female patients,the difference was statistically significant (χ2 =4.35,P <0.05 ).Conclusion HLA -B27 antigen has a high value for clinical diagnosis of ankylosing spondylitis,which is worthy of promotion.

might be related to the growth and metastasis of HCC.

Objective To research effects of water extraction of Salvia miltiorrhiza in sperm quality of mice. Methods Forty adult male Kunming mice were randomly divided into 5 groups, including low concentration group, medium concentration group, high concentration group, control group and positive control group in eight mice in each group. The low, medium and high concentration groups were given by gavage with 1 500, 3 000 and 6 000 mg/kg water extraction of salvia miltiorrhiza once a day respectively, while the control group was given gavage with 10 mL/kg normal saline daily, as well as the positive control group was injected with 100 mg/kg cyclophosphamide once; All mice were sacrificed after 30 days. We measured the weight gain, viscera coefficient and sperm quality in each mouse. Results There were no statistical difference in the weight of every week, increase of the weight, testis and epididymis coefficients among the control group, the low, medium and high concentration groups (P > 0.05), while the weight and weight increase were significantly higher than that in the positive control group (P < 0.05). Sperm concentration, viability, morphology, motility, PR, VSL, VAP of low, medium and high concentrations group were significantly improved than the control group (P < 0.05), and the sperm quality in the four groups were higher than that in the positive control group (P < 0.05). Conclusion Water extraction of salvia miltiorrhiza can obviously improve the sperm viability, morphology, and motility of mice.

Objective:To obtain purified recombinant Litopenaeus vannamei allergen protein Lit v 1.2.Methods: The target gene of Lit v 1.2 was inserted into clone vector pGEM-T and then ligated to the expression vector pET 44a.The pET44a-Liv 1.2 was transformed into Rosetta and screened by ampicillin resistance .The recombinant protein was expressed by IPTG induction .The protein was purified by 6-His tag affinity chromatography and the purification was analyzed by SDS-PAGE gel electrophoresis .Results:The ex-pression plasmid pET44a-Lit v 1.2 was constructed.SDS-PAGE showed that expressed Lit v 1.2 was efficient and soluble in E.coli Rosetta.The protein molecular weight was consistent with the theoretical value .The highly purified target protein was obtained.Conclusion:In this study ,we successfully gained highly purified recombinant allergen protein Lit v 1.2 which was expressed in prokaryotic system and purified by affinity chromatography column .The purified Lit v 1.2 protein will facilitate us to further study its role in immunological responses .

Objective To prepare cognitive assessment questionnaire on nursing ethics. Methods The methods of the research applied interview and face cross-sectional survey,prelimi-nary test paper was formed on the basis of the original research papers,and formal assessment test was formed with 17 questions.Using stratified sampling,468 nurses aged 19~53 years were col-lected from 4 first-class hospitals,reliability and validity assessment were tested through factor analysis regression and other statistical analysis methods.Results Nurses in nursing work on ethics problem of cognitive assessment table eventually confirmed 17 items,internal consistency co-efficient of the questionnaire was 0.922,and internal alpha coefficients of 2 factors were 0.901 and 0.921.By factor analysis,basic nursing operation,protection of privacy and self-supervision 2 factors were extracted and they could explain that the total variance was 53.589%,and project load value was 0.517~0.758.Conclusion The nurses to ethics problems in nursing work cogni-tive assessment scale have good reliability and validity,which can be used to study the nursing ethics.

Objective To prepare cognitive assessment questionnaire on nursing ethics. Methods The methods of the research applied interview and face cross-sectional survey,prelimi-nary test paper was formed on the basis of the original research papers,and formal assessment test was formed with 17 questions.Using stratified sampling,468 nurses aged 19~53 years were col-lected from 4 first-class hospitals,reliability and validity assessment were tested through factor analysis regression and other statistical analysis methods.Results Nurses in nursing work on ethics problem of cognitive assessment table eventually confirmed 17 items,internal consistency co-efficient of the questionnaire was 0.922,and internal alpha coefficients of 2 factors were 0.901 and 0.921.By factor analysis,basic nursing operation,protection of privacy and self-supervision 2 factors were extracted and they could explain that the total variance was 53.589%,and project load value was 0.517~0.758.Conclusion The nurses to ethics problems in nursing work cogni-tive assessment scale have good reliability and validity,which can be used to study the nursing ethics.

The 28th International Conference on Amyotrophic Lateral Sclerosis (ALS)-Motor Neuron Disease was held in Boston from December 8 to 10, 2017. The conference covered 23 topics, 102 special topics and 446 papers. This article briefly introduces some topics of the conference, involving basic research, clinical research and clinical trials. Among these, basic studies include genetics, cell biology and pathology, and superoxide dismutase1 gene ALS related pathology; clinical studies include the progression of ALS disease, cognitive behavioral disorders, and biological markers.

OBJECTIVE:To study induction effect of euphornin on the apoptosis of cervical cancer Hela cells and its mechanism. METHODS:The cervical cancer Hela cells were divided into blank control group,cisplatin group(positive control, 10 mg/L) and euphornin low-dose,medium-dose and high-dose groups (50,100,200 mg/L). They were treated with relevant medicine. The inhibitory effect of Hela cells proliferation was tested by MTT assay after 24,48,72 h of medicine treatment. The apoptotic rate of Hela cells was measured by flow cytometry after 48 h of medicine treatment. Morphology of nucleus was detected by Hoechst 33258 staining. The protein expression of Cyt-C,Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9 and Caspase-10 were detected by Western blot assay. RESULTS:Compared with blank control group,inhibitory rate of cell proliferation and cell apoptosis rate were increased significantly in cisplatin group and euphornin groups(P＜0.05 or P＜0.01),and obvious staining, deformation,shrinking,fragmentation or apoptotic bodies was found in nucleus. Compared with blank control group,the protein expression levels of Cyt-C,Caspase-8 and Caspase-9 in euphornin low-dose,medium-dose and high-dose groups were increased significantly,while the protein expression level of Bcl-2 and Bcl-2/Bax ratio were decreased significantly(P＜0.05 or P＜0.01);the protein expression levels of Bax,Caspase-3 and Caspase-10 in euphornin medium-dose and high-dose groups were increased significantly(P＜0.05 or P＜0.01). CONCLUSIONS:Euphornin can significantly inhibit the proliferation of Hela cell and promote cell apoptosis,the effect of which will be achieved by activating the Caspase-dependent mitochondrion apoptosis pathway.

Objective To analyze the clinical characteristics,prognosis and molecular biological changes of tonsillar squamous cell carcinoma(TSCC).Methods Retrospective analysis of 61 TSCC cases treated from January 1999 to December 2012.Demographic data and clinical charts,including histologic grade of tumor,treatment and outcome of the patients,were reviewed.Human papillomavirus (HPV)-DNA were detected using SPF10-DNA enzyme immunoassay and LiPA genotyping method.Expressions of p16 and p53 proteins were examinated by immunohistochemistry.Survival rate was calculated with SPSS 19.0 software using the Kaplan-Meier method.Results There were 55 males and 6 females,with a median age of 57 years.Of the 61 TSCC,21 were with well differentiation,19 with moderate differentiation and 21 with poor differentiation,including 7 patients at stage Ⅱ,10 at stage Ⅲ and 44 at stage Ⅳ.HPV-positive rate of TSCC was 29.5％ (18/61) and high-risk HPV-16 subtype accounted for 72.2％ (13/18).The percentage of famel patients in HPV-positive TSCC was higher than HPV-negative TSCC(22.2％ vs 4.7％).HPV-positive TSCC was more common in non-smoking patients (50.0％ vs 79.1％,x2 =5,155,P =0.023) and non-drinking patients (27.8％ vs 51.2％,x2 =4.346,P =0.037).HPV-positive TSCC mostly presented with high expression of p16 protein (88.9％ vs 16.3％,x2 =28.481,P =0.000),and low expression of p53 protein(72.7％ vs 46.5％,x2 =5.028,P =0.025).The prognosis of patients with HPV-associated TSCC was significantly better than non-HPV-associated TSCC,and The 3-year and 5-year overall survival rates of patients with HPV-positive TSCC were higher than those of patients with HPV-negative TSCC (87.7％ vs 49.5％ and 78.9％ vs 33.0％,respectively).Conclusion HPV-associated TSCC had unique clinicopathological and molecular biological features,showing better prognosis compared to HPV-negative TSCC.

Objective:To explore the value of pressure-strain loop (PSL) for non-invasive quantitative assessment of left ventricular myocardial work index (GWI), global work efficiency (GWE), global constructive work (GCW), global wasted work (GWW) in the evaluation of left ventricular myocardial work in patients with hypertrophic cardiomyopathy (HCM).Methods:Thirty-one HCM patients (HCM group) and 36 healthy volunteers (control group) from December 2018 to September 2019 in Henan Provincial People′s Hospital were selected. Relevant clinical data were collected, left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVEDd), left ventricular end systolic diameter (LVEDs), left atrial diameter (LAD), the maximum wall thickness (MWT), left ventricular mass index (LVMI), the global longitudinal strain (GLS), the peak strain dispersion(PSD) and GWI, GWE, GCW and GWW between the two groups were compared.Results:Compared with the control group, MWT, LAD, E/e′, LVMI in HCM group were significantly increased (all P<0.05); left ventricular myocardial functional parameters of GLS, GWI, GWE, GCW were significantly decreased, and GWW and PSD were significantly increased (all P<0.05). Left ventricular GLS, PSD and GWI, GCW, GWW, GWE have better repeatability within the observer and between observers. ICC within the observer were 0.852, 0.707, 0.917, 0.955, 0.675, 0.618, respectively. And their ICC between observers were 0.837, 0.631, 0.927, 0.944, 0.555, 0.670, respectively. Correlation analysis showed that GLS was positively correlated with GWI, GWE, and GCW ( r=0.765, 0.737, 0.815; all P<0.001), and negatively correlated with GWW and PSD ( r=-0.517, -0.606; all P<0.001). Conclusions:The left ventricular GWI, GWE and GCW decreased in HCM patients, while the GWW increased. PSL can evaluate the damage of left ventricular myocardial work in HCM patients.