1.Thoughts of Some Ethical Problems in Elderly Diabetes Mellitus
Xiaoning BAI ; Minquan HOU ; Huifang WANG
Chinese Medical Ethics 1995;0(04):-
Objective To observe some current situation in elderly Diabetes Mellitus with ethics in China,and to explore the method to solve the problems.Methods To analyze the problems with ethics.Results To advance some methods for solving the ethical problems associated with doctors and nurses,family and society.Conclusions Only by improving the minds of medical ethics of doctors and nurses,contributing infinite patience,attention and care,offering elderly diabetes mellitus patients much more help and ethical care with their family and society,they can get better clinical diagnosis and treatment,spiritual consolation,life care and economic support.
2.Spontaneous neoplastic lesions of Wistar rats and their incidences
Yanfeng XU ; Fenxia HOU ; Huifang YANG ; Yuhuan XU
Acta Laboratorium Animalis Scientia Sinica 2014;(2):75-79
Objective To investigate the spontaneous neoplastic lesions and their incidences in rats .Methods Sixty male and 60 female specific pathogen-free Wistar rats (4-weeks old ) were used in this study .The rats were acclima-ted for 1 week prior to initiation of the experiment .They were fed with conventional feed for 104 weeks and then sacrificed for histopathological examination .Results Various neoplastic lesions of the rats and their incidences were analyzed and reported.For male rats, their total tumor incidence was 49.12%, the benign tumor incidence was 38.60%and the malig-nant tumor incidence was 17.54%.The benign neoplastic lesions mainly were pituitary adenoma ( 19.30%) , testis Leydig cell tumor (5.26%) and subcutaneous fibroma (5.26%).The malignant neoplastic lesions mainly were squamous cell carcinoma (7.02%) and lymphoid hematopoietic system tumors (3.51%).For female rats, their total tumor inci-dence was 60.34%, the benign tumor incidence was 50.00%and the malignant tumor incidence was 15.52%.The benign neoplastic lesions mainly were breast fibroadenoma (25.86%) and pituitary adenoma (24.14%).The malignant neoplas-tic lesions mainly were adenocarcinoma (5.17%) and breast cancer (3.45%).Conclusions The spontaneous neoplastic lesions and their incidences reported in this paper provide another data of the spontaneous tumors of SPF Wistar rats and may provide some reference for relevant technical staffs .
3.Invariant nature killer T ( iNKT) cell percentages and subsets in non-obese diabetic ( NOD)/LtJ mice with different stages of type 1 diabetes
Dongzhi CHEN ; Xiaolin YIN ; Jialin LIU ; Huijuan ZHAO ; Yuanyuan WANG ; Huifang LIU ; Ming MENG ; Minghui HOU
Chinese Journal of Microbiology and Immunology 2018;38(5):327-336
Objective To detect and analyze the percentages of CD4+T, CD8+T and invariant na-ture killer T ( iNKT) cells as well as iNKT subsets in different tissues and organs of non-obese diabetic (NOD)/LtJ mice before the onset and in the early and late stages of type 1 diabetes (T1D) for better under-standing the immune function in different disease stages. Methods Female NOD/LtJ mice were selected as experimental subjects. Their fasting blood glucose levels were measured by blood glucose meter. Glycosuria and blood glucose level ≥11. 1 mmol/L in two consecutive detections were used as the diagnostic criteria of T1D. These mice were divided into three groups as follows: non-onset, early stage and late stage groups. Changes in food and water intake, glucose level in the urine, body weight, mental state, fur color and urine volume were recorded. Percentages of CD4+T, CD8+T and iNKT cells and ratios of subsets in peripheral blood, thymus, spleen, liver and inguinal lymph nodes were detected by flow cytometry (FACS). Results (1) Compared with the non-onset and the early stage groups, mice in the late stage group were apathetic and had rough hair. Moreover, significantly increased water and food intake and urine output (P<0. 05) and de-creased body weight, thymus index, spleen index and the absolute lymphocyte counts of spleen, liver and thymus (P<0. 05) were observed in the late stage group. (2) Compared with the non-onset group, the early stage group showed significantly increased percentages of CD4+T cells in spleen, liver, thymus and inguinal lymph nodes (P<0. 05). Compared with the early stage group, the late stage group showed decreased per-centages of CD4+T cells in liver, thymus, inguinal lymph nodes and peripheral blood (P<0. 05). Compared with the non-onset group, the percentages of CD8+T cells in the early stage group were significantly increased in spleen and thymus, but reduced in inguinal lymph nodes (P<0. 05). Compared with the early stage group, the percentages of CD8+T cells in late stage group were significantly reduced in liver and thymus, but increased in inguinal lymph nodes (P<0. 05). (3) The percentages of iNKT cells in liver and inguinal lymph nodes of mice in the early stage group were significantly higher than those of the non-onset group (P<0. 05). The percentages of iNKT cells in peripheral blood and liver of mice in the late stage group were sig-nificantly lower than those of the early stage group (P<0. 05). No significant difference in the percentages of iNKT cells in spleen and thymus was found among the three groups (P>0. 05). (4) Compared with the non-onset group, the percentages of iNKT1 subset in thymus in the early and late stage groups were significantly increased, while the percentages of iNKT2 subset were significantly decreased (P<0. 05). No significant difference in the percentages of iNKT1 and iNKT2 subsets in spleen, liver and inguinal lymph nodes was found among the three groups (P>0. 05). (5) The percentages of iNKT2 subset in spleen, liver and ingui-nal lymph nodes were significantly lower than those of the iNKT1 subset in the three groups (P<0. 05). The percentage of iNKT2 subset in thymus was significantly higher than that of iNKT1 subset in the non-onset group (P<0. 05). (6) Compared with the non-onset and the late stage groups, the early stage group showed significantly increased levels of IFN-γ, IL-4 and IL-17A and up-regulated ratio of IFN-γ/IL-4 (P<0. 05). Compared with the non-onset and the early stage groups, the late stage group showed significantly increased IL-6 level (P<0. 05). Compared with the non-onset group, IL-10 level in the other two groups was in-creased, especially in the late stage group (P<0. 05). No significant difference in IL-2 level was found among the three groups (P>0. 05). Conclusion Increased percentages of iNKT cells and iNKT1 subset in NOD/LtJ mice with early stage of T1D might be involved in the development of T1D.
4. Determination of titanium dioxide in the air of workplace by inductivehy coupled plasma optical emission spectrometry
Haibin LI ; Shuang SONG ; Xingfu PAN ; Xuewen HOU ; Huifang YAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(6):469-471
Objective:
To establish the method for determination of titanium dioxide in the air of workplace by inductivehy coupled plasma optical emission spectrometry (ICP-OES) .
Methods:
The titanium dioxide was collected by filter membrane and then digested by microwave digestion apparatus in the mixed solvents (HNO3∶HF∶H2O=4∶1∶1) , dilutedto 25 ml and detected by ICP-OES.
Results:
The sampling efficiency was higher than 95%; the linearity of ICP-OES was good at the range of 10-500 μg/ml, the minimum quantitation concentration was 0.72 mg/m3 (as collecting 150 L air sample) , the maximum quantitation concentration was 21.7 mg/m3 (as collecting 960 L air sample) , the recovery was ranged from 99.0%-102.0%, the
5.Advantage of dual-phase contrast-enhanced multi-slice computed tomography in intracranial tuberculosis
Huifang QU ; Dailun HOU ; Xu ZHANG ; Jisheng YANG ; Xiaoji YUAN ; Cheng LIU
Chinese Journal of Neuromedicine 2014;13(12):1207-1211
Objective To explore the superiority of dual-phase contrast-enhancement multi-slice computed tomography (MSCT) in observing intracranial tuberculosis.Methods Thirty patients with intracranial tuberculosis,admitted to our hospital from January 2003 to November 2011,were chosen in our study; regular 16-slice spiral CT scan was performed,and then,dual-phase MSCT was performed as follows:contrast-enhanced scan was performed 25 seconds after arrival of contrast material on vascular phase,and 5 minutes after that (lag phase),conventional scanning was performed.According to the different lesions and morphologies of the tuberculosis,they were divided into three types:meningeal thickening,meningeal tuberculoma and parenchymal tuberculoma; the lesion sizes and edge definition and the relationship of the lesions with the surrounding vascular lesions in these three types were scored and calculated,and the differences of the image quality were compared at the vascular phase and lag phase.Results A total of 526 lesions in 30 patients were found,including 22 with meningeal thickening,235 with meningeal tuberculomas/tubercles,and 269 with parenchymal tuberculomas/tubercles.As compared with these three types at the vascular phase (0.36±0.49,0.36±0.52 and 0.41±0.53; 0.00,0.27±0.45 and 0.12±0.32; 1.09±0.68,1.22±0.74 and 1.27±0.75),these three types at the lag phase had significant differences in the scores of lesion sizes (1.64±0.58,1.64±0.58 and 1.59± 0.60) and lesion edge definition (2.00,1.73±0.49 and 1.88±0.34) and the relationship of the lesions with the surrounding vascular lesions (1.82±0.39,2.00±0.06 and 2.00±0.06,P<0.05).Conclusion Images in the lag phase have advantages on diagnosis of intracranial tuberculosis.
6.Effects of attribution therapy on self-efficacy and physical function among patients with rheumatoid arthritis
Huifang LI ; Feifei HOU ; Luoyi LI
Chinese Journal of Practical Nursing 2022;38(7):519-524
Objective:To investigate the attribution therapy on self-efficacy and physical function among patients with rheumatoid arthritis, and provide reference for functional rehabilitation of patients.Methods:A total of 72 rheumatoid arthritis patients of Rheumatology Department of Henan Province Orthopaedic Hospital were divided into experimental group ( n=36) and control group ( n=36) by random digits table method. The control group received routine nursing, while experimental group implemented attribution therapy for 6 times. Before and after 3 months of intervention, the intervention effect was assessed by using Health Assessment Questionnaire (HAQ), Clinical Disease Activity Index (CDAI), morning stiffness time as well as Arthritis Self-efficacy Scale (ASES), respectively. Results:After intervention, the scores of HAQ, CDAI were 0.62 ± 0.23, 9.52 ± 3.15, morning stiffness time were (31.76 ± 5.80) minutes in the experimental group, significantly lower than those in the control group (0.91 ± 0.33, 12.41 ± 2.70) points and (35.91 ± 7.90) minutes. The differences were statistically significant ( t=4.09, 4.04, 2.46, all P<0.05). The scores of self-efficacy pain subscale, self-efficacy function subscale, self-efficacy other symptoms subscale and total ASES scores were 6.58 ± 1.20, 5.88 ± 1.36, 6.67 ± 1.90 and 6.30 ± 0.81, significantly higher than those in the control group (5.97 ± 0.76, 5.18 ± 1.24, 5.59 ± 1.73 and 5.59 ± 0.74), the differences were statistically significant ( t values were 2.21-3.77, P<0.05). Conclusions:Attribution therapy can effectively alleviate physical function and promote self-efficacy of patients with rheumatoid arthritis.
7.Phenotypic and genetic characteristics of a child with 7p15 deletion syndrome.
Jing WU ; Binghua DOU ; Ge MENG ; Huifang WANG ; Yaqin HOU ; Junke XIA ; Ying BAI ; Xiangdong KONG
Chinese Journal of Medical Genetics 2020;37(8):855-858
OBJECTIVE:
To explore the genetic basis for a child with multiple malformation and growth retardation.
METHODS:
The child was subjected to low-coverage massively parallel copy number variation sequencing (CNV-seq) based on next generation sequencing (NGS) technique.
RESULTS:
G-banding karyotyping analysis has found no abnormality in the boy and his parents. CNV-seq analysis discovered that the child has carried a heterozygous 4.36 Mb deletion (24 020 000-28 380 000) at 7p15.3p15.1. The same deletion was not found in either parent. The deletion has encompassed 28 OMIM genes including HOXA13, CYCS, DFNA5, HOXA11 and HOXA2. Among these, HOXA13 has been associated with distal limb deformity, hypospadias and cryptorchidism. HOXA1, HOXA3 and HOXA4 are involved in the formation of cardiac primordia and primordial tube, and HOXA2 is involved in the development of auditory system. The clinical phenotype of the child was consistent with that of 7p15 deletion syndrome.
CONCLUSION
Haploinsufficiency of HOXA1, HOXA2, HOXA3, HOXA4 and HOXA13 genes may underlie the clinical phenotype of the child, which is comparable to 7p15 deletion syndrome.
8.Expression of microRNA-373 in cutaneous squamous cell carcinoma and its effect on cell invasion
Yonghua XIA ; Huifang HOU ; Min LI ; Caifeng ZHANG ; Dong LIU ; Hua HU ; Mengjie ZHANG ; Sai CHENG
Chinese Journal of Dermatology 2017;50(10):715-718
Objective To investigate the expression of microRNA-373 (miR-373) in cutaneous squamous cell carcinoma (CSCC) tissues and cells,and to explore its effects on cell invasion.Methods Real-time PCR was performed to determine the expression of miR-373 in CSCC tissues and paralesional normal skin tissues,as well as in CSCC cell lines (A431 and SCL-1) and HaCaT cells.A431 cells were divided into 4 groups:miR-373 mimic group,miR-373 inhibitor group and negative control group which were transfected with miR-373 mimic,miR-373 inhibitor and negative control miRNA respectively,and untreated group receiving no treatment.Cell invasion assay was performed to evaluate effects of miR-373 downregulation on cell invasion.Western blot analysis was conducted to assess effects of miR-373 downregulation on the protein expression of matrix metalloproteinase-2 (MMP-2) and MMP-9.Results Expression of miR-373 was significantly higher in the CSCC tissues (2.465 ± 0.218) than in the paralesional normal skin tissues (1.000 ± 0.000,P < 0.05),and higher in SCL-1 cells (1.864 ± 0.178) and A431 cells (2.919 ± 0.277) than in HaCaT cells (1.000 ± 0.000,P < 0.05).Most notably,miR-373 expression was also markedly higher in metastatic CSCC tissues than in non-metastatic CSCC tissues (3.323 ± 0.344 vs.1.914 ± 0.161,t =4.158,P =0.000 4).Compared with the untreated group and negative control group,the miR-373 mimic group showed significantly increased miR-373 expression and invasive ability,while the miR-373 inhibitor group showed markedly decreased miR-373 expression and invasive ability (all P < 0.05).Conclusion MiR-373 downregulation can significantly suppress the invasion of A431 cells,and obviously decrease the protein expression of MMP-2 and MMP-9.
9.Factors affecting regular monitoring in patients with chronic myeloid leukemia who received tyrosine kinase inhibitor
Yingling ZU ; Yanli ZHANG ; Jian ZHOU ; Fengkuan YU ; Huifang ZHAO ; Ruirui GUI ; Zhen LI ; Yujie HOU ; Yongping SONG
Journal of Leukemia & Lymphoma 2019;28(1):39-42
Objective To observe regular monitoring in patients with chronic myeloid leukemia (CML) who received tyrosine kinase inhibitor (TKI), and to analyze its influencing factors. Methods A total of 857 patients with CML in Henan Tumor Hospital from October 2012 to October 2016 were collected. Patients were told to receive regular monitoring after receiving TKI treatment, including blood routine, bone marrow, BCR-ABL fusion gene and chromosomes. All patients were divided into good and poor compliance groups according to regular monitoring. Chi-square test was used to compare ABL kinase domain mutations rate and mortality between two groups. TKI species, level of education, duration from diagnosis to treatment, teaching times, sites of follow-up, convenience of transportation, annual income and gender were recorded respectively, and the factors affecting regular monitoring were analyzed by using single and multiple factor analysis. Results There were 390 and 467 patients in good and poor compliance groups respectively. Treatment failure rate was 19.49% (76/390) and 25.91% (121/467) in good and poor compliance groups respectively, the mutation rate was 28.95% (22/76) and 7.44% (9/121) respectively. The difference of ABL kinase domain mutation in patients with treatment failure of both groups was statistically significant (χ 2 =16.287, P < 0.01). The mortality was 0.77% (3/390) in good compliance group, and 2.78% (13/467) in poor compliance group, and the difference was statistically significant (χ 2 = 4.543, P = 0.033). The single factors analysis showed that TKI species, level of education, duration from diagnosis to treatment, teaching times, sites of follow-up, convenience of traffic and annual income were related with regular monitoring (all P < 0.05). Multiple-factor analysis showed that inconvenient transportation (β = 1.56, 95% CI 1.74-3.74, P = 0.014), low education level (β = 1.67, 95% CI 0.81-3.12, P = 0.041) and low income (β = 2.87, 95% CI 1.31-4.51, 95%CI 1.74-3.74, P = 0.011) were independent factors for poor compliance in regular monitoring. In the result detection, 56 fusion genes fluctuated. Conclusions CML patients who received regular monitoring have a low treatment failure rate and mortality. Inconvenient transportation, low education level and low outcome are independent risk factors for regular monitoring. The single monitoring result can not prompt treatment effect, and thus it needs to review and monitor for many times.
10.Introduction of guidelines for iodine thyroid blocking
Chunhui CHANG ; Ximing FU ; Huifang CHEN ; Long YUAN ; Yuhan HOU ; Lin HE ; Cuiping LEI
Chinese Journal of Radiological Health 2021;30(3):253-257
During a radiological or nuclear emergency, iodine thyroid blocking is an urgent protection action to prevent or reduce the absorption of radioactive iodine by thyroid. Although potassium iodide (KI) administration is recommended by WHO guidelines for iodine prophylaxis following nuclear accidents and is also widely implemented in most national guidelines, the scientific evidence for the guidelines lacks as the guidelines are mostly based on expert opinions and recommendations. This paper introduces the development and revision of WHO guidelines for iodine thyroid blocking published in 1989 and 1999, as well as the revision methods and main recommendations of the guidelines published in 2017, which supports the use of iodine thyroid blocking during a radiological or nuclear emergency.