Neurohypophyses of six adult rats were observed under electron microscope toinvestigate the morphological features of pituicytes and their possible functionalsignificance.The results of morphological observation showed that the pituicytesmay be divided into 4 types,the two of which,astral pituicytes and ependymalpituicytes,obviously resemble to the neurogliocytes of central nervous system intheir morphological features.The third,dark pituicytes,was rich in mitochondria.Little was known about secretory pituicytes other than their presumable secretoryfunction suggested by the secretory granules present in their cytoplasm.The interstitial cisternae were the sites where the neurosecretory activities carriedon.The dilations of nerve fiber around the interstitial cisternae were rich in vesi-cles.It was substantiated that the pituicytes can participate in supporting andnourishing the nerve fibers,removing cell debris and may also serve a function oftransferring excitation,because of the presence of gap junction between pituicytes andynapselike contact between pituicyte and the nerve fiber.

The frequency of peripheral lymphocyte subsets using monoclonal was studied in patients with viral myocarditis (MC). The percentage of T_8 positive cells was significantly lower in patients with MC of heart failure(MCHF) than that in patients with MC of arrhythmias and that in normal controls, whereas the T_4/T_8 ratio was significantly inereased in MCHF patients. At the same time, suppressive T cells (Ts) function in patients with MC was evaluated by shortlived assay. The Ts function was significantly reduced in MCHF patients, close correlation was found between the Ts function and the hemodynamic function in MCHF patients. Abnormal immunoreguiation may be of the important pathogenic factor in MCHF patients.

Objective To evaluate the efficacy of video-assisted mini-thoracoscopy(VAMT) for radical resection of lung cancer.Methods From April 2002 to December 2008,radical resection of lung cancer was performed on 39 patients by VMAT in our hospital.A 1.5-cm and a 7-to 10-cm incision were made during the operation.Both standard surgical instruments and thoracoscopic set were used to treat the pulmonary vessels,perform lobectomy,and remove the lymph nodes in the mediastinum and pulmonary portal.Results The operation was completed in all of the cases.No peri-operative death occurred.The patients received chest drainage for 4 to 7 days after the operation(mean,4.5 days).Post-operative pathological examination showed primary non-small cell lung cancer(NSCLC) in 37 cases,metastatic cancer in 1,and carcinoid in 1.In the 37 patients with NSCLC,9 were stage Ⅰa,13 were Ⅰb,5 were Ⅱa,7 were Ⅱb,2 were Ⅲa,and 1 was Ⅲb.Follow-up was available in 35 patients for up to 2 to 12 months in 4,13 to 24 months in 15,25 to 36 months in 8,37 to 48 months in 6,and 49 to 60 months in 2 cases.Among the 35 cases,totally 4 patients died of tumor recurrence or metastasis,1 patient survived with tumor,and the other 30 survived without tumor.The 1-and 3-year survival rate of stage Ⅰ and Ⅱ were 100%(21/21) and 91%(10/11) respectively.All of the 3 stage Ⅲ patients died of recurrence or metastasis in 19,11,and 14 months respectively after the surgery.Conclusions VAMT is effective for radical resection of lung cancer in a short term.Combination of traditional procedure and VAMT is safe and reliable for the disease.

Type B insulin resistance (TBIR) is a rare disease caused by the presence of insulin receptor autoantibodies (IRA).African middle-aged women are predisposed to it.Most patients have an underlying autoimmune disease,most commonly systemic lupus erythematosus (SLE).The patients predominantly present with abnormalities of glucose homeostasis ranging from extreme insulin resistance and symptomatic hyperglycemia to lifethreatening hypoglycemia,usually accompanied with manifestation of insulin resistance such as acanthosis nigricans,hyperandrogenism,and polycystic ovary.The diagnosis can be established by the elevation of IRA.We herewith report a ease of TBIR complicated with SLE,and the clinical features,diagnosis and treatment of TBIR are reviewed.

Objective To explore the guidance value of cerebrospinal fluid(CSF)and serum procalcitonin(PCT)in the ventricular catheter indwelling time of intracranial pressure monitoring(ICP).Methods Fifty-eight cases patients with moderate and severe craniocerebral trauma who were given ICP ventricular catheter and external ventricular drainage(EVD)were selected,5 ml CSF and 2 ml venous blood were collected at operation,1st,7th,14th d after operation.The change of CSF and serum PCT and WBC levels were compared among different time,the correlation among each indicators and diagnosis value for intracranial infection were analyzed.Results The level of CSF and serum PCT and WBC increased with the lengthen of ventricular catheter indwelling time(CSF PCT:(0.09±0.02)μg/L of operation,(1.17±0.25)μg/L of 14 d after the operation;CSF WBC:(24.33±12.75)×106/L of operation,(431.52±140.26)×106/L of 14 d after the operation;serum PCT:(0.16±0.05)μg/L of operation,(4.57±1.41)μg/L of 14 d after the operation;serum WBC:(4.14±0.46)×106/L of operation,(14.24±3.05)×106/L of 14 d after the operation;P＜0.05).The CSF PCT was positively correlated with CSF WBC and serum PCT(r=0.614,0.711,P＜0.05).The diagnostic sensitivity of CSF and serum PCT for prognosis of intracranial infection were 95.2％,81.0％,the specificity were 94.6％,78.4％,the specificity of CSF PCT was significantly higher than that of serum PCT(P=0.041).Conclusion CSF and serum PCT has high early diagnosis value for intracranial infection in patients with ICP ventricular catheter,which can contribute to guide the reasonable timing of tube drawing,brings out the best of ICP and cerebrospinal fluid drainage,improve the level of treatment in patients with traumatic brain injury.

Objective: To analyse the level of glucose concentration in saliva and to study the relationship between the level of blood glucose and that of salivary glucose.Methods: Blood glucose level and the glucose concentration in unstimulated mixed saliva taken from testee were measured with Beckman SYNCHRON CX7 System in 60 patients with diabetes mellitus and 60 healthy subjects. Results: The average value of salivary glucose concentration in experimental group was (1.950?0.179) mmol/L, that in control group was (0.953?0.124) mmol/L(P

Objective:To study the effect of insulin therapy on glucose concentration in saliva in patients with diabetes mellitus(DM), and to study the relationship between blood glucose level and salivary glucose level.Methods: Glucose concentration in blood and in unstimulated mixed saliva was measured with Beckman SYNCHRON CX7 system in 40 DM patients before and after insulin therapy.Results:The average value(mmol/L) of salivary glucose concentration before and after insulin therapy was 2.081?0.287 and 1.571? 0.193 respectively (P

Objective: To examine the microbial flora within the root canals with failed root canal therapy by PCR test, to identify detection rate of bacteria and the predominant bacteria species, and to determine the association of the various species with clinical features. Methods: Forty roots with failed root canal therapy were selected for this study. According to clinical features the roots were divided into three groups: pain, fistula and symptomless. After removal of the root filling material, the microbial samples were taken from the canal and detected by PCR test. Results: The microbial flora within root canals of teeth with failed root canal therapy was found to be mixed, six kinds of bacteria were detected. Enterococcus faecalis was the most commonly recovered bacteria species. Significant associations were observed between Prevotella nigrescens and pain, while dependablity was observed between Actinomyces israelii and fistula. Conclusion: The main cause of root canal treatment failure is the persistent microorganisms. The composition of bacteria in teeth with failed root canal therapy has its own specificity.

Objective To discuss the role of interleukin (IL)-12 in capillary bronchiolitis disease.Methods Fifty-nine cases of capillary bronchiolitis children under 2 years old were enrolled as the bronchiolitis group Ⅰ(n =28) and bronchiolitis group Ⅱ(n =31),36 cases of children with bronchopneumonia of the same age and 31 cases of children suffered from non-infectious diseases such as hernia and renal calculus of the same age were enrolled as bronchopneumonia control group and normal control group respectively.The peripheral blood IL-12 levels of four groups were detected by enzyme-linked immunosorbent assay.Results The levels of the peripheral blood IL-12 in bronchiolitis group Ⅰ,bronchiolitis group Ⅱ,bronchopneumonia control group and normal control group were (34.72±7.96) pg/ml,(55.30 ±6.72) pg/ml,(56.79±10.36) pg/ml and (61.23 ± 11.51) pg/ml respectively.The level of the peripheral blood IL-12 in bronchiolitis group I was significantly lower than that in bronchiolitis group Ⅱ,bronchopneumonia control group and normal control group respectively (P ＜0.05),and the level of the peripheral blood IL-12 in bronchiolitis group ⅡⅡ was significantly lower than that in bronchopneumonia control group and normal control group respectively (P ＜ 0.05),but there was no significant difference between bronchopneumonia control group and normal control group (P ＞ 0.05).Conclusion The level of IL-12 is one of the important factors for bronchiolitis disease,the reducing of serum level of IL-12 in children who have a high risk factor of capillary bronchiolitis is more obvious.

With light and electron microscope, we studied the morphology of the thymus of the neonatal mouse. The results showed: 1.the lobules of the thymus had not well developed and there was no distinct demarcation between the cortex and medulla; 2.a cyst composed of epithelial cells with microvilli or cilia might be frequently seen in the medulla; 3.occasionally small lymphocytes with some glycogen particles in their cytoplasm were observed; 4.only a few small-sized thymic corpusles existed in the medulla, The article also described the ultrastructure of the lymphocytes, epithelial reticular cells, macrophage, interdigitating cell and blood-thymus barrier in the thymus of the neonatal mouse.