2.Clinicopathological and molecular genetic characteristics of childhood diffuse large B cell lymphoma.
Hui HUANG ; Wen-ping YANG ; Hong-yan XU
Chinese Journal of Oncology 2012;34(3):209-211
Adolescent
;
Antigens, CD20
;
metabolism
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
Burkitt Lymphoma
;
drug therapy
;
metabolism
;
pathology
;
surgery
;
Child
;
Child, Preschool
;
Cyclophosphamide
;
therapeutic use
;
DNA-Binding Proteins
;
metabolism
;
Doxorubicin
;
therapeutic use
;
Female
;
Follow-Up Studies
;
Genes, myc
;
Humans
;
Hyaluronan Receptors
;
metabolism
;
Ki-67 Antigen
;
metabolism
;
Lymphoma, Large B-Cell, Diffuse
;
drug therapy
;
genetics
;
metabolism
;
pathology
;
surgery
;
Male
;
Neoplasm Staging
;
Neprilysin
;
metabolism
;
Peritoneal Neoplasms
;
drug therapy
;
genetics
;
metabolism
;
pathology
;
surgery
;
Prednisone
;
therapeutic use
;
Proto-Oncogene Proteins c-bcl-2
;
metabolism
;
Proto-Oncogene Proteins c-bcl-6
;
Stomach Neoplasms
;
drug therapy
;
genetics
;
metabolism
;
pathology
;
surgery
;
Translocation, Genetic
;
Vincristine
;
therapeutic use
3.Metatropic dwarfism in a case.
Ji HUANG ; Li-fu GUAN ; Hui-ping SHI ; Wei YU
Chinese Journal of Pediatrics 2003;41(4):310-310
4.The adiponectin level in gingival crevicular fluid in patients of chronic periodontitis with diabetes mellitus type 2
Daozhou LIU ; Wanhong WU ; Hui JIANG ; Fan ZHANG ; Ping HUANG
Journal of Practical Stomatology 2016;32(4):565-568
Objective:To examine the adiponectin level in gingival crevicular fluid(GCF)in patients of chronic periodontitis with dia-betes mellitus type 2.Methods:20 patients of diabetes mellitus type 2 with chronic periodontitis(DM&CP),20 of periodontitis(CP) and 20 health subjects(H)were included.The periodontal indexes (SBI,PLI,PD and AL)were measured,GCF samples were quan-tified by periotron 8000,the adiponectin content in GCF was tested by adiponectin ELISA kit.The relationship between the adiponectin level in GCF and the periodontal indexes of the DM&CP patients was analyzed statistically.Results:The adiponectin level in GCF in group DM&CP was significantly lower than that in the other 2 groups(P <0.05).The adiponectin levels in GCF in group CP and H were not statistically different.The adiponectin level in GCF was negatively correlated with PD and AL(P <0.05),but had no correlation with SBI and PLI(P >0.05).Conclusion:Decrease of adiponectin in GCF may play a role in the development of DM&CP.
5.Comparison of oxycodone and dezocine for prevention of fentanyl-induced cough during anesthesia induction
Meihua HUANG ; Hui WANG ; Jin XU ; Ping LI
Chinese Journal of Anesthesiology 2015;35(7):787-789
Objective To compare oxycodone and dezocine for prevention of fentanyl-induced cough during induction of anesthesia.Methods One hundred fifty patients of both sexes, aged 25-60 yr, weighing 45-75 kg, of ASA physical status Ⅰ or Ⅱ , scheduled for elective surgery under general anesthesia, were randomly divided into 3 groups (n =50 each) using a random number table: dezocine group (group Dez), oxycodone group (group Oxy), and normal saline control group (group NS).In group Dez, dezocine 0.1 mg/kg (in 10 ml of normal saline) was injected intravenously, and 5 min later fentanyl 3 μg/kg was injected over 5 s.In group Oxy, oxycodone 0.1 mg/kg (in 10 ml of normal saline) was injected intravenously, and 5 min later fentanyl 3 μg/kg was injected over 5 s.In group NS, normal saline 10 ml was injected intravenously, and 5 min later fentanyl 3 μg/kg was injected over 5 s.The occurrence and degree of cough were observed within 2 min after administration of fentanyl.Results The incidence of cough was 2%, 4% and 30% in Oxy, Dez and NS groups, respectively.Compared with group NS, the incidence of cough was significantly decreased, and the degree of cough was mitigated in Oxy and Dez groups.There was no significant difference in the incidence and degree of cough between Dez group and Oxy group.Conclusion Both intravenous oxycodone and dezocine 0.1 mg/kg can significantly prevent fentanyl-induced cough during induction of anesthesia with similar efficacy.
6.Mechanism underlying intrauterine growth retardation induced by caffeine and its research advance
Shu ZHOU ; Jing HUANG ; Chong BAO ; Jie PING ; Hui WANG
Chinese Journal of Pharmacology and Toxicology 2010;24(1):77-80
Intrauterine growth retardation (IUGR) is one of the most commonly encountered developmental toxicity, which could lead to perinatal morbidity and mortality, be also extended from the fetus to adulthood, and seriously affect the quality of the population. Caffeine widely exists in a variety of daily beverages and some drugs. Its consumption is increasing year by year. Caffeine intake during pregnancy is one of the risk factors for IUGR. However, its mechanism of adverse outcome based on embryonic research is still unclear. In this paper, the possible mechanisms of caffeine-induced IUGR focusing on 3 important factors-the mother, placenta and fetus were explored. Caffeine's impact on the mother is the chronic activation of renin-angiotensin system; on the placenta, caffeine induces cell damage or the failure of the cell proliferation/apoptosis balance, leading to blockage of blood supply to the placenta; caffeine is also capable of directly affecting fetal development through interfering its neuroendocrine.
7.Relationship between plasma level of fibrinogen and carotid atherosclerosis in the patients with ischemic cerebrovascular disease
Ping QV ; Hui HUANG ; Qiong ZHANG ; Guihai CHEN
Chinese Journal of Neurology 2008;41(6):367-370
Objective To investigate the association between the plasma level of fibrinogen and carotid atherosclerosis(CAS)in patients with ischemic cerebrevascddar disease(ICVD),and the effects intensity of different risk factors on CAS.Methods Carotid intima-media thickness(IMT)was measured by color Doppler uhrasonography in 840 patients with ICVD,including cerebral infarction(ci),transient ischemic attack and insufficient blood-supply of vertebral-basilar artery.According to the results of uhrasonography,the patients were divided into four groups:normal(IMT≤0.9 mm),arterial sclerosis (0.9 mm
8.Simultaneous determination and pharmacokinetic studies of ferulic acid and paeoniflorin in human serum by high performance liquid chromatography after oral administration of Modified Xiao-yao Decoction.
Hui LI ; Ping REN ; Xi HUANG ; Wenfu TANG ; Hongqiang WEI
Journal of Integrative Medicine 2008;6(11):1178-83
To simultaneously determine the contents and explore the pharmacokinetics of ferulic acid and paeoniflorin by high performance liquid chromatography (HPLC) after oral administration of Modified Xiao-yao Decoction (MXYD), a compound of traditional Chinese herbal medicine.
9.Metastasis and micrometastasis in ultra-low rectal cancer
Xuefeng GUO ; Meijin HUANG ; Ping LAN ; Hui PENG ; Jianping WANG
Chinese Journal of General Surgery 2009;24(5):402-405
Objective To study cancer metastasis in mesorectum and ischiorectal loss in cases of ultra-low rectal cancer and evaluate the rationale of Miles procedure. Methods Whole-mount slice and tissue mieroarray technique were used to study the dissected specimen from 23 cases of uhra-low rectal cancer for metastatic lymph nodes. Result 415 lymph nodes were harvested in 23 mesorectum specimen, 169 and 59 lymph nodes were metastasic and micrometastasie respectively. 12 eases were diagnosed with metastasis, 4 cases were found to have micrometastasis. Metastatic lymph nodes in the lateral and anterior mesorectum were 29.0% (49/169) and 17.2% (29/169) respectively. There were 2 patients with metastasis and 1 with micrometsstssis in ischiorectal fossa lymph nodes, accounting for 13% patients. Conclusion Regional metastasis exists in ultra-low rectal cancer and its incidence varies in different location of mesorectum and ischiorectal fessa. The value of Miles procedure as the standard therapy for ultra-low rectal cancer should undergo an evaluation.
10.Establishment and preliminary application of real time PCR assay for quantitative detection of CRLF2
Jingjing FU ; Hong LI ; Lijun YI ; Ping YUE ; Hui HUANG
International Journal of Laboratory Medicine 2015;(24):3520-3521,3524
Objective To establish a real‐time quantitative PCR method for the detection of cytokine receptor‐like factor 2 (CRLF2) expression .Methods Specific primers amplification target gene CRLF2 and housekeeping genes ABL were designed ,the purified PCR products were performed the TA cloning .After bacterial colony PCR screening and sequencing ,then the recombinant plasmids DNA was extracted and measured by using UV spectrophotometer and converted to copies/mL concentration .Finally it was diluted for preparing the plasmid standard substance ,then the standard curve was drawn for observing the sensitivity and linear rang ,meanwhile the stability of the plasmid DNA was evaluated .This method was initially applied to detect the CRLF2 level of bone marrow mononuclear cells in 10 cases of healthy children and 10 cases of newly diagnosed acute lymphoblastic leukemia (ALL) .Results CRLF2 PCR product had a single specific melting curve;the linear detection range of the standard substance was 103 - 108 copies /ml;the plasmid standard substance by freeze‐thawing for 3 times remained stable;the CRLF2 level of clinical sample was within the linear detection range of standard substance .Conclusion The real‐time quantitative PCR method for CRLF2 established by our laboratory has good specificity ,linearity range and stability ,which can be applied to the quantitative detection of CRLF2 gene in clinical ALL children .