Objective To establish a method for rapid determination of chlorinated benzene compounds in drinking water.Methods Chlorinated benzene compounds were extracted by purge-and-trap and separated by GC then determined by MS.Results The correlation coefficients of 6 chlorinated benzene compounds were 0.999 1-0.999 8 and the average rates of recovery were 93.0%-97.5%(n=6).The relative standard deviation was 2.45%-4.36%(n=6) and limit of detection 0.007-0.05 ?g/L.Conclusion The method established in the present paper is simple,precious,accuracy and sensitive and applicable to the determination of chlorinated benzene compounds in water.

Objective To assess the value of enhanced multislice spiral CT (MSCT) scan using fat-dense enema for the staging of colorectal carcinoma.Methods 33 cases with colorectal carcinoma confirmed by colonoscopy were examined by enhanced MSCT scan ,from the dome of the diaphragm to symphysis pubica,after bowel cleaning,administration of smooth muscle relaxant, and rectal fat-dense contrast agent insuffation .Carcinomas were staged with TNM by axial,multiplannar reconstruction(MPR) and CT virtual endoscopy (CTVE) images. The staging results on CT were compared with that of postoperative pathological examination in all cases.Results All the 33 colorectal carcinoma were demonstrated clearly by MSCT ,the total accurate rate in staging with the TNM classification was 78.78%.The sensitivity and positive accurate values for T staging were 100%and 87.88%(29/33),respectively. The sensitivity and positive accurate values for in detecting lymph node involvement were 86.36%(19/22)and 68.18%(15/22), respectively. Five cases in M stage were all diagnosed correctly. Conclusion Using fat-dense contrast medium and enhanced multislice spiral CT is of very important value in staging of colorectal carcinoma.

To investigate whether the use of concentrate growth factors have a favorable impact on clinical outcome of immediate implant in periodontally infected sites.48 patients with 51 teeth with periodontitis and periapical periodontitis accepted immediate implantation in the maxillary esthetic zone.25 implants(test group) were placed simultaneously with guided bone regeneration (GBR) using CGF combined with Bio-oss, other 26 implants(control group) were treated with Bio-oss alone.The facial bone thickness at 0, 4 and 8mm apical to the implant platform were measured immediately after, 3 months after and 6 months after surgery;pain visual analogue scale (VAS) was used to assess the degreeof pain in the patient within 1 week after surgery.3 months after surgery,the change of horizontal resorption in labial was less in test group(P<0.05).In the first three days postsurgery, the test group reported significantly less pain with respect to the control group(P<0.05).The application of CGF produced beneficial impacts on clinical outcome of immediate implant in periodontally infected sites.

Objective To compare the clinical effects of four kinds of gingival retraction technique in treat-ment of anterior teeth restoration.Methods 169 patients for 352 teeth with tooth body and tooth dentition defect by anterior crown bridge repair were chosen and divided into 4 group by patients'order,including A group(88cases)with single track method,B group(91cases)with single line for two times method,C group(89cases)with double line method and D group(94cases)with double line for two times method.And the fixed restoration satisfaction rate of anterior teeth of both groups was compared.Results The satisfaction rates of gums,shoulder,impression,model and restoration of A group were 73.9%,70.1%,69.3%,65.9%,61.4% respectively,which of B group were 83.5%, 80.2%,80.2%,80.2%,76.9%;which of C group were 84.3%,80.9%,78.7%,77.5%,76.4%;which of D group were 94.6%,93.6%,92.6%,92.6%,90.4%.There were no significant differences in the fixed restoration satisfaction rate of anterior teeth between B group and C group(χ2 =2.43,1.17,2.85,3.04,2.30,all P >0.05). The fixed restoration satisfaction rate of anterior teeth of D group were significantly better than A,B and C groups (χ2 =14.90,16.39,15.83,19.87,21.38,all P <0.05 ).Conclusion Double line for two times method in the treatment of anterior teeth restoration can efficiently avoid ree gingival damage in the situation with abutment shoulder preparation,improve the quality of preparation,impression and model,and are helpful to higher the fitness of the neck of anterior teeth fixed restorations.

Objective To investigate the influence of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expression of adhesive and invasive molecules. Methods With an artificial CO2 pneumoperitoneum model in vitro, human gastric cancer cells MKN-45, SGC-7901 and MKN-28 were exposed to 3 different CO2 gradients: 9 mm Hg, 15 mm Hg and control group (0 mm Hg). The expression of E-cadherin, ICAM-1, MMP-2 and VEGF-A were measured at 2 and 4 hours exposure by using RT-PCR, CytoMatrixTM kit and ECMatrixTM kit. The pretreated gastric cancer cells were injected into abdominal cavity of nude mice(2×106 cells per mouse). Five mice in each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity. The remaining mice were kept for observation of survival time. Results The expression of E-cadherin (MKN-45: from 2.26 to 2.19, SGC-7901 :from 2.16 to 2.09、MKN-28 :from 2.06 to 1.99), ICAM-1 (MKN-45 : from 2.20 to 2.28、SGC-7901: from 2.10 to 2.18、MKN-28: from 2.00 to 2.08), MMP-2 (MKN-45:from 2.05 to 2.13、SGC-7901: from 1.95 to 2.03、MKN-28: from 1.85 to 1.93) and VEGF-A(MKN-45 : from 2.10 to 2.16、SGC-7901 :from 2.00 to 2.06、MKN-28: from 1.90 to 1.96) didn't change significantly with increasing pressure and time (P>0.05). The expression of adhesive and invasive molecules didn't change significantly between the experimental groups and the control group. There was no statistical significance of tumor metastasis in abdominal cavity of nude mice(MKN-45:from 22 to 23、SGC-7901 :from 20 to 22、MKN-28:from 21 to 22) and survival time(MKN-45 :from 23 to 21、SGC-7901 :from 22 to 21、MKN-28 :from 22 to 21) among all the groups. Conclusion Under low pressure and short time of CO2 exposure, the adhesive and invasive capacity of gastric cancer cells did not change significantly hence did not increase the possibility of neoplasm metastasis.

Aged ; Diabetes Mellitus, Type 2 ; therapy ; Humans ; Walking

Animals ; Biological Availability ; Drug Carriers ; chemistry ; Drug Delivery Systems ; Humans ; Lactic Acid ; chemistry ; pharmacokinetics ; Nanoparticles ; Polyesters ; Polyethylene Glycols ; chemistry ; pharmacokinetics ; Polyglactin 910 ; chemistry ; pharmacokinetics ; Polyglycolic Acid ; chemistry ; pharmacokinetics ; Polymers ; chemistry ; pharmacokinetics

The plastic splint PCL,which is introduced in this article,is a new type of medical exter- nal immobilization material.During the course of its development,the burden has been optimality seeked with regressive orthogonality after screening of the material.Its function has attained the international levels during the eighties.This splint may be used not only in the treatment of burns and various orthopedic conditions,but also in the immobilization of fracture and rehabilation treatment of orthopedic patients etc.

Objective To investigate the expression and clinical significance of serum response factor (SRF) and vascular endothelial growth factor receptor (VEGFR2) in gastric carcinoma. Methods SABC immunohistochemical method was used to determine the expressions of SRF and VEGFR2 in 50 cases of gastric carcinoma, 50 cases of surgery incisal edges and 29 cases of lymph node metastasis focus. Results The detection positive rates of SRF and VEGFR2 in gastric carcinoma were 52.00 % (26/50) and 60.00 %(30/50), respectively, which were significantly higher than those in the normal gastric mucosa [16.00 % (8/50) and 10.00 % (5/50), respectively] (P< 0.05), with no statistical difference with metastiatic lymph node [65.52 % (19/29) and 72.41 % (21/29), respectively]. In the gastric carcinoma group, the expression of SRF was relevant with depth of invasion and lymph node metastasis (P<0.05). The expression of VEGFR2 was not related to age, gender and tumour size (P>0.05), but closely correlated to differentiation degree, invasion depth and lymph node metastasis (P<0.05). The expressions of SRF and VEGFR2 in the gastric carcinoma were positively correlated (r= 0.594, P< 0.05). Conclusion Overexpressions of SRF and VEGFR2 in gastric carcinoma can be regarded as the poorly prognostic markers and play an important role in invasion and metastasis of gastric carcinoma.

Objective To explore the clinical characteristics and prognosis factors of child hemophagocytic syndrome(HPS)in different age groups.Methods Eighty-five children cases of HPS were divided into 3 age groups,0-2 years old (group A),＞2-8 years old (group B) and ＞8-14 years old (group C).The etiology,pathology,clinical symptoms,laboratory detection and prognosis were retrospectively analyzed.Results In the etiology,the etiology in the group A,B and C was dominated by EB virus infection,the infection positive rate in the group C was significantly higher than that in the group B and C;in the bone marrow examination:the occurrence rate of granulocytes to erythrocytes ratio ＜1.0 in the group A was higher than that in the group C;in the clinical symptoms,cervical lymph node enlargement mostly occurred in the group B,hepatosplenomegaly was most rare in the group C,and the serous cavity effusion commonly occurred in the group B;in the laboratory detection,the LDH level in the group B was higher than that in the group A,the above differences were statistically significant(P＜0.05).There were no statistically significant differences in other clinical symptoms and laboratory detection results among the three groups (P＞0.05);In the comparison between the survival group and death group,the serous cavity effusion,triglyceride(TG),CD4/CD8 ratio and granulocytes to erythrocytes ratio in bone marrow had statistical difference(P＜0.05).The above factors were performed the Logistic analysis,the results showed that TG＞3.5 mmol/L,CD4/CD8 ratio ＜1.24 and bone marrow granulocytes to erythrocytes ratio ＜1.02 were the unfavorable risk factors affecting the prognosis of child HPS (P＜0.05).Conclusion Partial clinical characteristics of child HPS are related with the onset age,TG level,CD4/CD8 ratio and bone marrow granulocytes to erythrocytes ratio are the risk factors affecting prognosis.