Euthyroid Sick Syndromes ; blood ; etiology ; Female ; Gestational Age ; Humans ; Hypothyroidism ; blood ; etiology ; Infant, Newborn ; Infant, Premature ; Male ; Risk Factors ; Sex Factors ; Thyronines ; blood ; Triiodothyronine ; blood

Animals ; Birds ; Influenza A Virus, H5N1 Subtype ; Influenza in Birds ; virology

Endothelial Cells ; metabolism ; pathology ; Humans ; Sepsis ; metabolism ; physiopathology

Acquired Immunodeficiency Syndrome ; prevention & control ; Adult ; Burnout, Professional ; Chi-Square Distribution ; Female ; Humans ; Male ; Medical Staff ; Middle Aged ; Rural Population ; Surveys and Questionnaires ; Young Adult

OBJECTIVE To investigate the relationship of Pseudomonas aeruginosa strain in hospital infection. METHODS ?-Lactamase gene,aminoglycoside modification enzymes(AMEs)gene,and resistant antiseptic(qacE△1-sul1)gene were detected to mutidrug resistant P.aeruginosa with polymerase chain reaction(PCR) and we conducted cluster analysis of the molecule marked drug resistance gene. RESULTS PCR showed the positive ratios of TEM,CARB and VIM genes were 24.32%,43.24%,and 5.4%,respectively,While SHV,CTX-M,OXA-10 group,PER,GES,VEB,DHA,and IMP genes were all negative,the deleted ratio of oprD_2 gene was 86.49%;the positive ratios of aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰand ant(2″)-Ⅰgenes were 8.11%,43.24%,18.91%,and 40.54%,respectively and the positive ratio of qacE△1 gene was 51.35%,There were clone transmitted phenomenon. CONCLUSIONS Hospital P.aeruginosa infection can induce clone transmitted hospital infection and it has fulminant prevalence.

Objective To explore the occurrence of anemia, anaemia type, iron metabolism situation,variation of serum Prealbumin (PA), and the relationship among them in cancer patients. Methods Three hundred and seventy cancer patients, admitted from July 2007 to April 2010 to our hospital, were enrolled into the study. The clinical data of all subjects were analyzed retrospectively. Results The incidence of anemia in 370 patients was 55.67% (206/370). Among all 206 anemia cases,129 cases had mild anaemia and 77 cases had middle to severe anaemia. In the last group ( n = 77 ), we found significant decrease in serum iron level ( [ 8. 37 ± 6. 09 ] μmol/L) and increase in serum ferritin level ( [ 474. 57 ± 327. 58 ] μg/L); and the correlation between serum iron and the anemia degree( Ps ＜ 0. 05 ). However, we found no significant differences of serum ferritin level between the groups with different degree of anemia(P ＞0. 05). Among all 206 anemia cases ,187(90. 77% ) patients had a low level of serum PA, but no relationship between the degree of anemia and the drop of serum PA ( P ＞ 0. 05 ). Conclusion The anemia was very popular in cancer patients, which had correlation with iron metabolism situation but not PA.

We reported a simple and fast fluorescence system based on quantum dots ( QDs ) to detect glutamate dehydrogenase ( GLDH) , which inverted glutamate to α-ketogrutarate using NAD+ as a coenzyme. The fluorescence of CdTe QDs was quenched by nicotinamide adenine dimucleotide ( NAD+) through an electron transfer pathway, and the quencher NAD+ could be consumed by adding NAD+-dependent enzymes and corresponding substrates. Based on this principle we introduced GLDH to consume NAD+ in the QDs/NAD+ system, leading to the enhancement of the fluorescence intensity of QDs, which was in proportional to the amounts of GLDH added. Using this fluorescence system, we measured GLDH in a wide concentration range from 10 U/L to 1000 U/L, which was of significance in clinical diagnosis of different kinds of liver diseases.

Humans ; Microcirculation ; Sepsis ; physiopathology

Adult ; Brain Injuries ; Foreign Bodies ; Humans ; Male ; Paranasal Sinuses ; injuries

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Rheumatic Diseases ; diagnosis ; drug therapy