2.Effects of recombinant human augmenter of liver regeneration on renal inflammation after renal ischemia reperfusion injury in rats
Xiaohui LIAO ; Hang SUN ; Qi LIU ; Hui GUO ; Ling ZHANG
Chinese Journal of Nephrology 2012;28(2):121-126
Objective To investigate the effects of recombinant human augmenter of liver regeneration (rhALR) on renal inflammation in acute kidney injury (AKI) induced by renal ischemia reperfusion (IR). Methods SD rats were randomly divided into sham-operated group,IR group,rhALR1 group (100 μg/kg) and rhALR2 group (200 μg/kg).Both renal pedicles of rats were identified and occluded with microvascular clamps for 60 min to induce acute kidney injury (AKI).Blood urea nitrogen and serum creatinine levels were evaluated using a Hitachi 747 automatic analyzer. For histological examination, sections were stained with HE. The activity of myeloperoxidase (MPO) was detected by spectrophotometer.Expression of TNF-α,ICAM-1,MCP-1 was determined by Western blotting. Results Blood urea nitrogen,serum creatinine levels and the injury of kidney were improved significantly in rhALR group as compared with IR group (all P< 0.05).They were improved more significantly in rhALR2 group as compared to in rhALR1 group (all P<0.05).The protein levels of TNF-α,ICAM-1,MCP-1 and the activity of MPO in kidneys from the sham-operated rats were low,and increased significantly after renal ischemia reperfusion injury (all P<0.05).After treated with rhALR,the expression of TNF-α,ICAM-1,MCP-1 and the activity of MPO were decreased significantly in kidneys as compared to those in IR group (all P<0.05),which decreased more significantly in rhALR2 group than those in rhALR1 group (all P< 0.05). Conclusions nhALR can protect kidneys from ischemia reperfusion injury in rats.The mechanism may be associated with the inhibition of renal inflammatory cells infiltration and down-regulated expressions of YNF-α,ICAM-1 and MCP-1 in the kidney.
3.Meta-analysis on the association of interleukin-13 gene polymorphism and the genetic susceptibility of asthma in Chinese children
Chaofeng XING ; Ling LI ; Yu HUI ; Yun GUO ; Jun QIAN
Chinese Journal of Applied Clinical Pediatrics 2015;30(4):295-300
Objective Published literatures on the relationship between IL-13 gene polymorphism and the susceptibility of children to bronchial asthma in China were comprehensively analyzed with the use of Meta-analysis to evaluate this relationship.Methods The data were collected from the Medline database,Ovid database,the Cochrane library,and Chinese Biomedical database,and the references of eligible studies were manually screened.Published data related to case-control studies reporting the link between IL-13 polymorphisms and asthma in Chinese children were retrieved through those database.Meta-analysis was conducted to determine whether the IL-13 gene polymorphisms were associated with asthma.Results Eighteen studies were finally accepted for analysis.There were three studies focusing on C-1 112T polymorphism,and six studies focusing on C + 1923T polymorphism,and fourteen studies focusing on G + 2044A polymorphism.There was no evidence to confirm that the genotypes in position IL-13-1112 C/T were associated with asthma in Chinese children [odds ratio(OR) =1.00,95% CI 0.82-1.22,P =0.98].The OR of asthma for TT/CC genotypes was 1.15 (95 % CI 0.57-2.33,P =0.69) and for CT/CC was 1.01 (95 % CI 0.82-1.25,P =0.89).There was significant evidence to confirm that the genotypes in position + 1923 C/T were associated with asthma in Chinese children(OR =1.86,95% CI 1.29-2.67,P =0.000 9).The OR of asthma for TT/CC genotypes was 2.12 (95 % CI 1.27-3.56,P =0.004) and for TC/CC was 1.67 (95% CI 1.18-2.35,P =0.003).There was no correlation between IL-13 + 2044G/A polymorphism and the susceptibility (OR =1.33,95% CI 0.94-1.88,P =0.11).The OR of asthma for AA/GG genotypes was 1.30 (95 % CI 0.76-2.20,P =0.34) and for AG/GG was 1.24(95% CI 0.90-1.70,P =0.19).Conclusions IL-13 gene + 1923 TT and TC genotypes should be associated with susceptibility of asthma in Chinese children,and the T allele could increase the risk of asthma.No clear relationship was found between the genotype TT/TC at the IL-13-1112 site and the incidence of asthma of children in China,and so was the genotype AA/AG at the IL-13 +2044 site and the incidence.
4.CD4+CD25+FoxP3+Treg in the immune mechanism of severe mycoplasma pneumoniae pneumonia in children
Yun GUO ; Jun QIAN ; Ling LI ; Yu HUI ; Hanmin JIANG
International Journal of Pediatrics 2016;43(8):647-650
Objective To investigate the role of CD4+CD25+FoxP3+ in severe Mycoplasma pneumonia among children.Methods One hundred and forty children with M.pneumoniae pneumonia (65 severe and 75 non-severe) who were hospitalized were enrolled along with forty other children as controls.X-ray was assessed.The proportions of peripheral blood CD4+CD25+FoxP3+cells were determined by flow cytometry.Results Both severe and non-severe children had decreased CD4+CD25+FoxP3+cells as compared with control subjects in acute phase (0.87 ± 0.66% vs.3.88 ± 2.00%,P < 0.01 and 1.17 ± 0.70% vs.3.88 ±2.00%,P <0.01,respectively).The levels of CD4+CD25+FoxP3+cells in severe children were lower than those in non-severe children in acute phase and recovery phase (0.87 ±0.66% vs.1.17 ±0.70%,P <0.05 and 1.66 ±0.85% vs.3.61 ± 1.45%,P<0.01,respectively).Both severe children and non-severe children expressed higher CD4+CD25+FoxP3+cells in recovery phase than in acute phase (1.66 ± 0.85 % vs.0.87 ± 0.66%,P <0.01 and 3.61 ± 1.45% vs.1.17 ±0.70%,P <0.01,respectively).Conclusion The expression of CD4+CD25+FoxP3+Tregs may play a role in the onset of severity of mycoplasma pneumonia and the low express of CD4+CD25+FoxP3+Tregs in children infected with M.pneumonia may increase the susceptibility to severe mycoplasma pneumonia.
5.Association of HLA-DR/DQ with Oral Lichen Planus
hui-yuan, LI ; ling-di, XU ; guo-yao, TANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(10):-
Objective To investigate the association of human leukocytes antigen(HLA)-DR/DQ with oral lichen planus(OLP) in the area of Yangtze River Delta. Methods HLA-DRB1 and DQB1 genotyping of 44 unrelated OLP patients and 150 normal controls were performed by polymerase chain reaction-sequence specific primers(PCR-SSP) method.The data were compared between the OLP group and normal controls,and between different types of OLP patients. Results The frequency of HLA-DRB1*09 and HLA-DRB1*07 alleles were significantly higher in OLP group than those in normal controls(56.8% vs 31.3% and 27.3% vs 13.3%,P
6.Application of negative pressure attraction method of brushing and washing in oral care of patients with orotracheal intubation
E FENG ; Yan-Ling YIN ; Xin-Rong GUO ; Hui-Ling ZHENG
Chinese Journal of Modern Nursing 2012;18(11):1280-1282
Objective To explore the effect of negative pressure attraction method of brushing and washing apply in oral care of patients with orotracheal intubation.Methods Totals of 82 oral intubation patients in ICU were randomly selected and divided into experience group ( n =42 ) and control group ( n =40 ).The traditional method of oral care was used in control group,while the negative pressure attraction method of brushing and washing was used in experience group,then,the incidence of oral infections,ventilator-associated pneumonia( VAP),dental plaque and oral mucosal injury were observed in two groups.Results The incidence of oral infections,ventilator-associated pneumonia(VAP),dental plaque in experience group respectively were 7.14%,2.38%,4.76%,and that in control group were 22.50%,15.00%,32.50%,the differences were statistically significant ( x2 =3.88,4.18,10.55,respectively; P < 0.05 ),while there was no significant difference in the incidence of oral mucosal injury between two groups ( P > 0.05 ).Conclusions The negative pressure attraction method of brushing and washing can effectively keep the oral clean of patients with orotracheal intubation,reduce the incidence of oral infections and ventilator-associated pneumonia and dental plaque,when compared with the traditional method of oral care.
7.Effects of pH9.0 Tris-EDTA and pH6.0 citrate on antigen retrieval.
Xin-Lan LUO ; Yan-Hui LIU ; Heng-Guo ZHUANG ; Xiu-Ling CAI ; Jie XU
Chinese Journal of Pathology 2005;34(11):749-751
Antigens
;
analysis
;
Cervical Intraepithelial Neoplasia
;
metabolism
;
Citric Acid
;
Cyclin-Dependent Kinase Inhibitor p21
;
analysis
;
Edetic Acid
;
Formaldehyde
;
Hot Temperature
;
Humans
;
Hydrogen-Ion Concentration
;
Immunohistochemistry
;
Intestinal Mucosa
;
immunology
;
Ki-67 Antigen
;
analysis
;
Microwaves
;
Palatine Tonsil
;
metabolism
;
Proto-Oncogene Proteins c-bcl-6
;
analysis
8.Influence of different antigen retrieval on the immunohistochemistry.
Xin-lan LUO ; Xiu-ling CAI ; Yan-hui LIU ; Heng-guo ZHUANG ; Wei ZHANG
Chinese Journal of Pathology 2005;34(1):52-54
Antigens
;
analysis
;
Autoantigens
;
analysis
;
Breast Neoplasms
;
metabolism
;
Citrates
;
Female
;
Formaldehyde
;
Hot Temperature
;
Humans
;
Hydrogen-Ion Concentration
;
Immunohistochemistry
;
methods
;
Iodide Peroxidase
;
analysis
;
Iron-Binding Proteins
;
analysis
;
Paraffin Embedding
;
Receptors, Progesterone
;
analysis
;
Thyroid Gland
;
immunology
;
Tissue Fixation
9.Effectiveness of manual therapy and traction for lumbar disc herniation: a meta-analysis.
Jiang-Hui BU ; Ling-Jun KONG ; Chao-Qing GUO ; Xiao-Cun YANG ; Ying-Wu CHENG
China Journal of Orthopaedics and Traumatology 2014;27(5):409-414
OBJECTIVETo evaluate the effectiveness of manual therapy and traction for lumbar disc herniation and analyze the current status of this kind of randomized clinical trial (RCT).
METHODSDatabase of CNKI, VIP, WANFANG, PubMed and OVID were searched. Some relevant journals were manually retrieved. A total of 2 874 literatures on manual therapy and traction for lumbar disc herniation were collected, of which 17 articles met the inclusion criteria. The Jadad score scale was used to evaluate the quality,and RevMan5.0 was used for meta-analysis of literatures.
RESULTSThe results of the meta-analysis of all trials involved were as followed:the combined effect of the effective rate was RR = 1.10, 95% CI [1.06, 1.14], the combined effect of the cure rate was RR = 1.36, 95% CI [1.21,1.52], the combined effect of the VAS was RR = 1.37, 95% CI [1.28, 1.45], the combined effect of the JOA was RR = 4.75, 95% CI [4.40, 5.09].
CONCLUSIONThe overall quality of the current RCT researches about manual therapy for lumbar disc herniation was lower,and did not support the conclusion that manual therapy was more effective than traction for lumbar disc herniation.
Humans ; Intervertebral Disc Displacement ; surgery ; therapy ; Lumbar Vertebrae ; surgery ; Musculoskeletal Manipulations ; methods ; Randomized Controlled Trials as Topic ; Traction ; methods
10.Application of shear wave elastography in the evaluation of neck-shoulder myofascial pain syndrome.
Ling GUO ; Chen ZHANG ; Ding-ding ZHANG ; Jing-hua GAO ; Guang-hui LIU ; Shang-quan WANG
China Journal of Orthopaedics and Traumatology 2016;29(2):142-145
OBJECTIVETo study clinical value of shear wave elastography (SWE) in the evaluation of neck-shoulder myofascial pain syndrome.
METHODSFrom December 2013 to July 2014,30 patients diagnosed as neck-shoulder myofascial pain syndrome were in the treatment group,including 17 males and 13 females, with an average age of (44 ± 3) years old. Thirty healthy people were in the control group, including 22 males and 8 females, with a mean age of (37 ± 5) years old. The patients in the treatment group were treated with manipulation, once every other day, total 7 times. The SWE was used to detect tension part of trapezius muscle of patients in the treatment group before and after treatment, as well as to detect muscle belly at the descending part of trapezius muscle in the control group. The tissue elasticity and Yang's modulus value were recorded and compared.
RESULTSThe tissue elasticity chart of patients in the treatment group before treatment was mainly greenish blue with the score of 3.70 ± 1.53, and the Yang's modulus was (43.4 ± 15.6) kPa. The tissue elasticity figure after treatment was mainly blue with the score of 2.40 ± 0.87, and the Yang's modulus was (29.0 ± 5.9) kPa. Whereas in the control group, the tissue elasticity figure was mainly blue with the score of 1.60 ± 0.72, and the Yang's modulus was (24.0 ± 7.6) kPa. These were statistical differences between the two groups (P = 0.000).
CONCLUSIONSWE can be used as an evaluation method of manipulation treatment for neck-shoulder myofascial pain syndrome, which is an objective and sensitive detection method.
Adult ; Elasticity Imaging Techniques ; methods ; Female ; Humans ; Male ; Middle Aged ; Musculoskeletal Manipulations ; Myofascial Pain Syndromes ; diagnosis ; therapy ; Neck ; Shoulder