1.The study of therapy of the hypoplastic myelodysplastic syndrome
Lin HUANG ; Hui-Hui LIU ; Hong WANG ;
Cancer Research and Clinic 2006;0(11):-
Hypoplastic myelodysplastic syndrome(Hypo-MDS),with the derangement of BM texture,is characterized by the hypoplasia of bone marrow(BM)accompanied with the deterioration of MDS. Hitherto there isn't a definite therapeutic definition about Hypo-MDS. Is therapy should be paid more attention to. This article introduces the study of therapy of Hypo-MDS.
2.A preliminary study of hyperoxic solution taken orally on fatigue induced by exercise and its mechanism
Hui ZHANG ; Chunran LIU ; Lin WANG
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
Objective To investigate the antifatigue effect of hyperoxia solution and its mechanism. Methods Eighty soldiers were randomly divided into two groups: hyperoxic solution group and common balance salt solution group The exercise selected was 5000 meters constantly running. The soldiers of each group were respectively given hyperoxic solution or balanced salt solution 250ml thirty minutes before running; then each were respectively given orally 150ml hyperoxia solution or balanced salt solution at 2000 meters and 4000 meters, The soldiers were bled before running and after running immediately, and the contents of lactic acid and malondialdehyde,the general ability of antioxidation and the energy of adenosine triphosphate enzyme were determined instantly. Results The contents of lactic acid and malondialdehyde of hyperoxic solution group were significantly less than that of control group ( P
3.Extracelluar matrix stimulates neurite outgrowth of dorsal root ganglion neurons differently depending on whether extracted from degenerated or normal intervertebral disk
Chinese Journal of Tissue Engineering Research 2014;(7):1039-1044
BACKGROUND:Compared with the normal intervertebral disk, the density of nerve fibers and number of nerve endings and neuropeptides appear to be more in the degenerated intervertebral disk. However, this phenomenon does not occur in the normal y aged disk.
OBJECTIVE:To evaluate the axonal growth and induction of a painful neuropeptide and substance P using rat dorsal root ganglion neurons and degenerated human disc cells in vitro.
METHODS:The human intervertebral discs were harvested from patients with discogenic low back pain and normal people. And extracelluar matrix extracted from human degenerative intervertebral discs was cultured with rat dorsal root ganglion neurons. The promotion of axonal growth and induction of substance P of dorsal root ganglion neurons in extracted medium were evaluated through morphology observation and enzyme-linked immunosorbent assay, respectively.
RESULTS AND CONCLUSION:Compared with the normal group, the content of nerve growth factor in the degenerative group was significantly higher and the average length of neuritis was significantly longer in the experimental group (P<0.05). After intervention with anti-nerve growth factorβ, the average length of neuritis became remarkably shorter. The percentage of substance P-immunoreactive cells was significantly higher in the degenerative group compared with the normal group (P<0.001). Nerve growth factors that highly express in the extracellular matrix from the degenerative intervertebral dick can promote neurite outgrowth of dorsal root ganglion neurons and induce release of neuropeptides related to pain transmission.
4.Evaluation of the efficacy and usefulness of low temperature hydrogen peroxide gas plasma sterilization system for endoscopies
Yanling CHEN ; Xueyan LIU ; Hui LIU ; Zhiyan LIN
Chinese Journal of Practical Nursing 2006;0(14):-
Objective To evaluate the efficacy of STERRAD (r) 100S (low temperature hydrogen peroxide gas plasma sterilization system) by comparing with formaldehyde steam. Methods From 2005.1 to 2005.3, totally sterilization of 2 760 pieces of operating tools were carried out with formaldehyde steam. From 2005.1 to 2005.3, totally sterilization of 4 950 pieces of operating tools in 180 circles were carried out with STERRAD (r) 100S. The instrument damage rate, operating infection rate and satisfaction rate of medical workers of 2 groups were compared. Results All data were in favor of the efficacy of STERRAD (r) 100S. Conclusions STERRAD (r) 100S is a safe, effective, low temperature, and cost-effective sterilization system..
5.Screening for Kaposi sarcoma-associated genes by using Genechip technology
Hui WANG ; Guodong LV ; Xiaodong WANG ; Yan HUI ; Hui LIU ; Renyong LIN ; Xing WANG
Chinese Journal of Dermatology 2011;44(6):403-406
Objective To screen for Kaposi sarcoma (KS)-related genes. Methods Tissue samples were obtained from the lesion and normal skin of a patient with KS in Xinjiang Uygur Autonomous Region,and total RNA was extracted from these samples and reverse transcribed into cDNA. Real-time fluorescent quantitative reverse transcription PCR (RT-qPCR) was performed to determine the expression of K8.1, K2 and ORF50 in these samples. The cDNA was labeled with fluorescein and hybridized to a human 35K genome array containing 25 100 genes. Subsequently, the signal images were scanned by a laser scanner and acquired images were analyzed by software. Results RT-qPCR revealed the mRNA expression of K8.1, K2 and ORF50 in the KS tissues but not in the normal skin tissues, indicating that there was no crossed contamination in these specimens. Among the 25 100 genes, 1313 genes were identified to be differentially expressed between KS and normal skin tissues, including 756 up-regulated genes and 557 down-regulated genes. These differentially expressed genes, such as myeloid cell leukemia-1 gene (MCI-1), annexins (ANX) and serine proteinase inhibitor Kazal type 5 (SPINK5), were associated with apoptosis, angiogenesis, cell signaling, protein processing, cell cycle regulation, and so on. Conclusion The differentially expressed genes such as MCI-1 and SPINK5 may be associated with the development of KS.
6.Treatment of intractable atlantoaxial dislocation with atlantoaxial pedicle screw instrumentation in children
Bin LIN ; Kejian LIAN ; Xiongwei DENG ; Zhimin GUO ; Hui LIU
Chinese Journal of Trauma 2008;24(8):608-611
Objective To explore the clinical effects of atlantoaxial pedicle screw instrumentation in treatment of intractable atlantoaxial dislocation in children. Methods A total of 7 patients with intractable atlantoaxial dislocations were treated with aflantoaxial pedicle screw instrumentation plus atlantoaxial bone grafting from June 2002 to January 2001. Results The dislocation in all patients reached complete reduction, with no complications. All patients were followed up for average 10 months (8-14 months). Radiographs showed successful bone fusion in all patients. Conclusion Atlantoaxial pedicle screw fixation and fusion is an effective method for treatment of intractable atlantoaxial dislocation in chil dren.
7.Establishment of a model of rabbit auricular hematoma and its application
Wenlang WEI ; Hui LIN ; Xuanyu WEI ; Xingxing DI ; Yongcheng LIU
Chinese Journal of Medical Aesthetics and Cosmetology 2012;(5):376-378
Objective To establish a rabbit auricular hematoma model,to observe the process of production and absorption,and to study the location of hematoma and method of its removal Methods Ten health New Zealand rabbits were divided randomly into control group and experimental group.A weight was dropped onto particular locality of auricles 3-6 times until a big hematoma appeared.The pathological processes of hematoma were observed,and the hematoma was formed 1 hour and the hematoma was almost absorbed after 4 days.The drainage experiment was conducted to observe the effect of needle aspiration or incision and drainage on the hematoma.Results All experimen tal auricles formed hematoma.The histopathological study showed that hematoma located between skin and cartilage,did not involve the cartilage.After 4 days,new-formed capillaries and fibroblasts were found,and then hematoma mostly absorbed; fibrous hyperplasia was been found.By cutting the hematoma,the blood clot could be thoroughly removed.Conclusions The auricular hematoma model can be established easily by striking with a heavy weight.Hematoma locates in subcutaneous connective tissue.A large hematoma should be early removed for prevention of auricular deformity.
8.Risk factors for nosocomial pneumonia in elderly stroke patients
Zhiguo RAO ; Zongming LIU ; Guijun LIN ; Hui GUO
Chinese Journal of Geriatrics 2008;27(7):518-520
Objective To investigate the risk factors for nosoeomial pneumonia in elderly stroke patients(aged 60 years and over). Methods The clinical data of 259 patients with nosoeomial pneumonia from Jan 2002 to June 2007 were collected and the risk factors were retrospectively analyzed. Results The morbility rate of nosocomial pneumonia in elderly stroke patients was 41.3%,and the risk factors were aging,Iong hospitalization,unconsciousness,type of stroke,and underlying diseases,smoking,tracheal intubation,tracheotomy,application of respiratory machine (all P>0.05). Conclusions The morbility rate of nosoeomial pneumonia in elderly stroke patients was high,and the risk factors are aging,long hospitalization,unconsciousness,type of stroke,and underlying diseases,smoking,tracheal intubation,tracheotomy,application of respiratory machine.
9.Exploration of the common problems facing the nursing trainees
Fengping LIU ; Yeqing ZOU ; Xiaoyan HUANG ; Hui LIN ; Faxiang LUO
Chinese Journal of Practical Nursing 2008;24(12):1-3
Objective To explore the common problems that facing the nursing trainees and seek the way to solving them. Methods The established Internet forum for nursing eases which was divided into six areas,such as clinical decision,ethical problems,nursing skills, the relationship between teachers and students,the relationship between nurses and patients,vocational prevention;77 nursing stuedents who were from three junior colleges of Jiangxi province participated in this study voluntarily.Nursing cases that happened during practical period were sent to the forum by volunteers and were discussed by nursing students and tutors. Results The cases sent by the nursing students were listed as the following order:relationship between teachers and students, relationship between nurses and patients, vocational prevention, clinical decision, ethical problem, and nursing skills. Conclusions During practical period, the main problems which the nursing students focused on stemod from the relationship between teachers and students, the relationship between nurses and patients and vocational prevention. Nursing educators should put emphases on the related knowledge and skills in order to improve the students'ability of coping with these problems.
10.Microsurgical treatment of cavernous hemangioma located in pons(report of 12 cases)
Feng GAO ; Wenyi ZHANG ; Hui GUO ; Zongming LIU ; Guijun LIN
Clinical Medicine of China 2008;24(11):1149-1151
Objective To analyze the mierosurgery treatment and prognosis of cavernous hemangioma loca-ted in pons. Methods 12 cases with cavernous hemangioma located in ports underwent mierosurgery. 11 lesions lo-cated in dorsal tons were resected through midline suboccipital trans- rhomboid fnssa approach. 1 lesion located in ventrolateral pous was resected with suboceipital retrosigmoid approach. Results All the 12 cavernous hemangiomns were rosected totally and confirmed by the postoperative pathology. Clinical improvement was gained in 6 cases, no change in 3 ,aggravation of facial palsy in 1 ,death in 1. The mean follow-up time was 3 months,and Mill scan dem-onstrated good restoration of brain stem tissue with no recurrence signs of lesion. The symptoms due to the operation recovered to some extent. Conclusion The cavernous hemangioma located in brain stem can be resected safely and effectively given the selection of surgical indication and optimal surgical approach.