Objective To investigate the effect of repeated and prolonged +Gz exposure on endocrine function of vessel in a rabbit model of atherosclerosis. Methods Twenty-four male New Zealand white rabbits were used to establish the model of atherosclerosis, and then randomly divided into control group and +Gz exposure group (12 each). Animals in control group underwent no +Gz exposure, while those in +Gz exposure group underwent +Gz exposure for 4, 8, and 12 weeks respectively (4 animals at each time point), and 4 animals from each group were sacrificed at each time point. The levels of angiotensin II (Ang II), endothelin (ET), heme oxygenase-1 (HO-1), carbon monoxide (CO) and cyclic guanosine-3', 5'-monophosphate (cGMP) were measured by radioimmunoassay and biochemical method, and the ultrastructure of aortic intima was examined by electron microscopy. Results Along with the prolongation of +Gz exposure, the contents of Ang II, ET, HO-1, CO and cGMP were increased in +Gz exposure group (P<0.05), but the increase stopped at the 12th week, and no obvious change in the above indices was observed in control group (P=0.05). The foam cells and collagen content under the aortic intima and in the shallow layer of aortic media were slightly increased in control group, but the foam cells and the interstitial collagen fibers were greatly increased after +Gz exposure for 8 and 12 weeks as compared with that in control group. Conclusion Repeated and prolonged +Gz exposure may induce the production of Ang II and ET, increase the secretion of HO-1, CO and cGMP, thus play a potential role in protecting the vessels from further injuries.

Abstract: Objective To analyze the changes of coagulation indicators in patients with hepatitis B complicated with Clonorchis sinensis (C. Sinensis), and provide reference value for diagnosis, drug using and prognosis monitoring. Methods　The patient samples were collected from the Third Affiliated Hospital of Sun Yat-sen University from June 2018 to February 2022 and divided into six groups. They were 40 healthy patients, 47 patients with simple chronic hepatitis B, 47 patients with post-hepatitis B liver cirrhosis, 40 patients with C. Sinensis mono-infection, 30 patients with chronic hepatitis B patients co-infected with C. Sinensis and 27 patients with post-hepatitis B liver cirrhosis patients coinfected with C. Sinensis. Four coagulation indexes, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB), were detected and compared among the groups. Results Compared with the healthy group, C. Sinensis mono-infection group had higher level of FIB and delayed PT, APTT; chronic hepatitis B group and chronic hepatitis B patients co-infected with C. Sinensis group had delayed PT, APTT, TT and significant lower FIB, these differences were statistically significant (all P<0.05). Compared with simple chronic hepatitis B group, post-hepatitis B liver cirrhosis and chronic hepatitis B patients co-infected with C. Sinensis had significant delayed PT, APTT, TT and lower FIB (all P<0.05). Compared with the post-hepatitis B liver cirrhosis and chronic hepatitis B patients co-infected with C. Sinensis, post-hepatitis B liver cirrhosis patients coinfected with C. Sinensis group had significant delayed PT, APTT, TT and lower FIB (all P<0.05). Conclusions The coinfection of C. Sinensis will further aggravate the coagulation dysfunction of HBV patients, leading to poor treatment and prognosis. HBV patients will have worse coagulation function in the process to post-hepatitis B liver cirrhosis; Therefore, it is important to pay attention to C. Sinensis co-infection when treating HBV patients, so that further guidance on clinical use and monitoring of prognosis can be provided.

Antigens ; chemistry ; Fixatives ; chemistry ; Humans ; Hydrogen-Ion Concentration ; Immunohistochemistry ; methods ; Microwaves ; Staining and Labeling ; methods

Antigens, CD34 ; metabolism ; Cytokines ; metabolism ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Mucin-1 ; metabolism ; Perineum ; pathology ; Phosphopyruvate Hydratase ; metabolism ; Sarcoma ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Aged ; Antigens, CD20 ; analysis ; Gene Rearrangement ; Humans ; Immunoblastic Lymphadenopathy ; genetics ; metabolism ; pathology ; Immunoglobulin Heavy Chains ; genetics ; Immunohistochemistry ; Lymph Nodes ; metabolism ; pathology ; Lymphoma, T-Cell ; genetics ; metabolism ; pathology ; Male ; Polymerase Chain Reaction ; RNA, Viral ; analysis ; Receptors, Antigen, T-Cell, gamma-delta ; genetics ; Reed-Sternberg Cells ; metabolism ; pathology

Objective To investigate the effect of repeated and prolonged +Gz exposure on endocrine function of vessel in a rabbit model of atherosclerosis. Methods Twenty-four male New Zealand white rabbits were used to establish the model of atherosclerosis, and then randomly divided into control group and +Gz exposure group (12 each). Animals in control group underwent no +Gz exposure, while those in +Gz exposure group underwent +Gz exposure for 4, 8, and 12 weeks respectively (4 animals at each time point), and 4 animals from each group were sacrificed at each time point. The levels of angiotensin II (Ang II), endothelin (ET), heme oxygenase-1 (HO-1), carbon monoxide (CO) and cyclic guanosine-3', 5'-monophosphate (cGMP) were measured by radioimmunoassay and biochemical method, and the ultrastructure of aortic intima was examined by electron microscopy. Results Along with the prolongation of +Gz exposure, the contents of Ang II, ET, HO-1, CO and cGMP were increased in +Gz exposure group (P<0.05), but the increase stopped at the 12th week, and no obvious change in the above indices was observed in control group (P=0.05). The foam cells and collagen content under the aortic intima and in the shallow layer of aortic media were slightly increased in control group, but the foam cells and the interstitial collagen fibers were greatly increased after +Gz exposure for 8 and 12 weeks as compared with that in control group. Conclusion Repeated and prolonged +Gz exposure may induce the production of Ang II and ET, increase the secretion of HO-1, CO and cGMP, thus play a potential role in protecting the vessels from further injuries.

Adult ; Fabry Disease ; pathology ; Female ; Follow-Up Studies ; Humans ; Kidney Glomerulus ; pathology ; ultrastructure ; Proteinuria

Aged ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cyclophosphamide ; therapeutic use ; Doxorubicin ; therapeutic use ; Epstein-Barr Virus Infections ; Female ; Herpesvirus 4, Human ; Humans ; Lymphoma, Large B-Cell, Diffuse ; drug therapy ; pathology ; virology ; Lymphoma, T-Cell, Peripheral ; drug therapy ; pathology ; Neoplasms, Second Primary ; drug therapy ; pathology ; Prednisone ; therapeutic use ; Vincristine ; therapeutic use

Adenocarcinoma ; diagnosis ; pathology ; Adult ; Aged ; Aged, 80 and over ; Ascitic Fluid ; chemistry ; Cadherins ; analysis ; Calbindin 2 ; Carcinoembryonic Antigen ; analysis ; Diagnosis, Differential ; Epithelial Cells ; chemistry ; Female ; Humans ; Immunohistochemistry ; Keratin-5 ; analysis ; Male ; Middle Aged ; Pericardial Effusion ; chemistry ; diagnosis ; Pleural Effusion ; chemistry ; diagnosis ; Pleural Effusion, Malignant ; chemistry ; diagnosis ; S100 Calcium Binding Protein G ; analysis

Abdominal Cavity ; Adult ; Anemia, Aplastic ; therapy ; Antigens, CD34 ; metabolism ; Carcinoma ; metabolism ; pathology ; Dendritic Cell Sarcoma, Follicular ; metabolism ; pathology ; Diagnosis, Differential ; Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Ki-67 Antigen ; metabolism ; Lymph Nodes ; pathology ; Male ; Neoplasms, Muscle Tissue ; metabolism ; pathology ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism ; Sarcoma, Kaposi ; etiology ; metabolism ; pathology ; Viral Proteins ; metabolism