The connotation development of industry and education is the road to advance the development of connotation and the quality of higher vocational and technical education.Based on this and bringing into play the advantages of running a vocational school and combined with cultivating the applied personnel needed by enterprises and professions,the college perfects the personnel cultivation model.carries out the "225" education model,accelerate the development of the teacher,promote its service capacity to enhance constantly the quality of higher vocational and technical education.

Objective To compare the results of three methods for measuring C reaction protein.Methods 100 patients were collected from our hospital,and three different methods for measuring C reaction protein were used to analyze the level of C reaction protein.Results Correlation analysis showed that the correlation between immunoturbidimetric assay and immunochromatography was higher.The differences of three methods assayed the C reaction protein were significant (P <0.05 )among normal people by the variance analysis,but had not statistical significance among abnormal people (P >0.05).The differences among all of test peo-ple were significant(P <0.05).Conclusion Detection of C reaction protein was important for diagnose inflammatory diseases.

Objective To observe the effects of dendrobium nobile Lindl(DNL) on Nuclear factor(NF)-κB and interleukin(IL)-6 expression of renal tissue in diabetic rat model .Methods The diabetes mellitus rat model was induced by streptozotocin .All rats were divided into four groups at random :the normal control group(group N) ,diabetic control group(group diabetes mellitus) ,drug group one(group DNL1) ,drug group two(group DNL2) ,and administrated for 12 weeks .The levels of the fasting plasma glucose , urea nitrogen ,serum creatinine ,urine creatinine ,24 hours urinary protein were determined .NF-κB and IL-6 protein expression levels were measured by Western-Blot .IL-6 mRNA expression levels were measured by Real-time fluorescence quantitative PCR . Results Compared with group N ,the blood sugar ,urea nitrogen ,24 hours urinary protein of group diabetes mellitus were signifi-cantly higher(P<0 .01) ,creatinine clearance was significantly reduced (P<0 .01) ,the NF-κB、IL-6 protein expression levels and IL-6 mRNA level increased significantly (all P<0 .05) .Compared with group diabetes mellitus ,the blood sugar ,urea nitrogen ,24 hours urinary protein of group DNL1 and group DNL2 were significantly decreased(P<0 .05) ,creatinine clearance increased significantly (P<0 .05) ,the NF-κB、IL-6 protein expression levels and IL-6 mRNA level decreased significantly(all P<0 .05) .Conclusion The DNL could protect the kidney of diabetic rats ,reduce the levels of NF-κB 、IL-6 protein and IL-6 mRNA expression in renal tissue of diabetic rats .

Aged ; Aneurysm, Dissecting ; therapy ; Aorta ; Catheterization ; methods ; Humans ; Male

Objective To determine the incidence of clinically common acute avulsions of the popliteus tendon (PT) and the lateral collateral ligament (LCL) of the femur (peel-off lesion) in knees with multiple injured ligaments.Methods A retrospective review of 48 acute grade 3 posterolateral corner (PLC) knee surgeries was conducted.All office evaluations were reviewed to determine the demographic data,tear description,preoperative MRI examination,physical examination under anesthesia,intraoperative arthroscopic findings and surgical techniques.Results In total,19 patients were surgically verified as having femoral peel-off lesions as a portion of a series of multiple pathologic findings.Tear patterns were typically categorized into 3 patterns:isolated PT tear (pattern 1,4 patients),combined PT and LCL tears (pattern 2,8 patients) and complex tears (associated intrasubstance and/or fibular-based injuries,pattern 3,7 patients).Seventeen of the 19 patients underwent preoperative MRI examination,which demonstrated conclusive signs of femoral insertion separation and discontinuity in 13 patients (76%) and inconclusive signs of femoral insertion separation.Examination under anesthesia (EUA) revealed that for the 4 patients with pattern 1 injuries,the tibial external rotation increases were all greater than 10°.For the 15 patients with pattern 2 and 3 injuries,the yarus instability was as follows:grade 3 in 8 patients,grade 2 in 2,grade 1 in 1,and negative in 4.External rotation increases were greater than 10° in 12 patients and less than 10° in 3.Seventeen of the 19 patients were inspected with an arthroscope prior to PLC surgery:of these patients,94% had acute avulsion findings and positive lateral gutter drive-through sign;8 patients were repaired with recess or reattachment procedures;6 patients were primarily reconstructed;4 patients were treated with combined repair and reconstruction;and 1 patient was left untreated for secondary reconstruction due to priority repair for concurrent patellar tendon rupture.Conclusion For adequate clinical identification,we recommend a comprehensive diagnostic protocol,including MRI,physical examination and arthroscopic inspection,to avoid underdiagnosis of the lesion in multiple-ligament injured knees.The lateral gutter arthroscopic evaluation is particularly sensitive for detecting the lesion.

Objective To develop a new method, capillary electrophoresis(CE) based on molecular beacon(MB),for rapid detection of polymerase chain reaction(PCR).To explor the roles of the IL-13 gene exon4 A2044G single nucleiotide polymorphism(SNP) in the pathogenesis of bronchial asthma.Methods The IL-13 exon 4 was amplified by PCR with genomic DNA used as templates from 20 healthy persons and 32 patients with dominantly allergic familial history living in the north of china.Then separating the amplification with capillary electrophoresis followed by hybridization of molecular beacons into the PCR product which were sequenced in the end. Results Detected with CE-MB method,there was significant difference in the distribution of A/G in IL-13exon 2044,A allele frequency was higher in asthma compared with normal controls,same as sequencing. Conclusion The method of capillary electrophoresis based on molecular beacon(MB-CE) is able to be developed as a clinical detecting method for genetic variation diseases.IL-13 A2044G SNP is important in the asthmatic mechanism.

Objective To investigate the clinical effects of lactobacillus acidophilus combined with glutathione in the treatment of nonalcoholic fatty liver disease.Methods 120 patients with nonalcoholic fatty liver disease were selected,and they were randomly divided into control group (60 patients used glutathione alone) and observation group (60 patients received lactobacillus acidophilus on the basis of control group).The clinical efficacy,liver function index,blood lipid index and the ultrasonography grading of fatty liver before and after treatment of the two groups were compared.Results The clinical total effective rates of the control group and the observation group were 76.67％,91.67％,respectively.The total effective rate of the observation group was significantly higher than that of the control group(x2 =10.52,P ＜ 0.05).The levels of AST,ALT and GGT of the observation group after treatment were significantly lower than those of the control group and before treatment [(36.89 ± 7.14) U/L,(36.89 ± 7.14) U/L,(36.89 ±7.14) U/L vs.(49.36 ± 11.08) U/L,(45.91 ± 10.24) U/L,(90.28 ± 20.70) U/L;(36.89 ± 7.14) U/L,(36.89 ± 7.14) U/L,(36.89 ± 7.14) U/L vs.(92.90 ± 24.3) U/L,(81.11 ± 17.37) U/L,(147.27 ± 34.19) U/L,t =2.88,2.54,2.91;3.01,3.36;3.18,3.48;3.41,3.87;all P ＜0.05).The levels of TG,TC,HDL-C and LDL-C of the observation group after treatment were significantly better than those of the control group and before treatment [(1.66 ± 0.42) mmol/L,(4.05 ± 0.77) mmol/L,(1.69 ± 0.60) mmol/L,(2.44 ± 0.38) mmol/L vs.(2.13 ± 0.80) mmol/L,(4.64 ± 0.94) mmol/L,(1.45 ± 0.48) mmol/L,(2.97 ± 0.57) mmol/L;(1.66 ± 0.42) mmol/L,(4.05 ± 0.77) mmol/L,(1.69 ± 0.60) mmol/L,(2.44 ± 0.38) mmol/L vs.(2.71 ± 1.33) mmol/L,(5.42 ± 1.27) mmol/L,(1.08 ± 0.36) mmol/L,(3.49 ± 0.71) mmoL/L,t =2.43.2.13,2.55,2.07;3.02,3.41;3.11,3.65;2.81,3.30;2.87,3.15;all P ＜ 0.05).In the control group,normal in 6 cases,mild in 24 cases,moderate in 21 cases and severe in 9 cases as fatty liver degree;in the observation group,normal in 13 cases,mild in 30 cases,moderate in 13 cases and severe in 4 cases as fatty liver degree.The ultrasonography grading of fatty liver of the observation group after treatment were significantly better than those of control group and before treatment (U =3.74,5.20,all P ＜ 0.05).Conclusion Lactobacillus acidophilus combined with glutathione in the treatment of nonalcoholic fatty liver disease can efficiently promote the recovery process of liver function,regulate blood lipid levels and is helpful to improve the imaging grading.

OBJECTIVE: To develop a new form named dropping core pills which contain both water soluble and liposoluble constituents of the traditional Chinese medicine compounds. METHODS: Volatile, liposoluble and water-soluble constituents were extracted with supercritical fluid extraction-CO2,ethanol extraction, water decoction and membrane separation and other methods respectively. The dropping core pills were prepared with liposoluble and volatile constituents before being coated with water-soluble constituent. RESULTS: As compared with watered pills and dropping pills, dropping core pills had the advantages of both but overcome their shortages in that the dosage form was optimized, the amount of the reagent less and the resolving time was shorter. CONCLUSION: Dropping core pill was a new dosage form suitable for traditional Chinese Medicine compounds in which liposoluble and water-soluble components were both active compounds.

Animals ; Disease Models, Animal ; Heart Rate ; Hypertension ; physiopathology ; Rats ; Rats, Wistar

Female ; Humans ; Polycystic Ovary Syndrome ; therapy