The paper conducts a case study of Tongji Medical College, Huazhong University of Science&Technology, introduces the application and management status of various cards, elaborates the one-card integration program for the management of staffs in the hos-pital and discusses the problems needing attention and the application effect.It is noted that the one-card solution facilitates the simpli-fication of the work process, the improvement of management efficiency and the reduction of management costs.

Objective In order to meet the needs of Medicare patients, hospitals use existing network equipment to implement the connection with Data Center of Medicare without changing the present frame. Methods Network Address Translation function (NAT) was used to translate equipment IP address that hospital needs to connect with data center of Medicare into legal IP address in data center of medicare. Results The author used existing NAT function of equipment to reduce the repeating purchase for network equipment and successfully realized the connection between hospital and Data Center of Medicare. Conclusion Using NAT devices not only does not need to change the local IP address but effectively overcomes the lack of legal IP addresses, and what's more, network security is further strengthened.

The paper described the main function modules, system architecture, key technology and reservation business process of the appointment register platform system; security measure of the reservation platform system, as evidenced by the access of third-party payment interface, multi-tier data service, digital safety network among others which are designed to ensure the security and operability of the system.

The effects of phorbol-12,13-dibuterate (PDBu) on total sodium current (I(Na)-total), tetrodotoxin-resistant sodium current (I(Na)-TTXr), 4-AP-sensitive potassium current (I(A)) and TEA-sensitive potassium current (I(K)) in trigeminal ganglion (TG) neurons were investigated. Whole-cell patch clamp techniques were used to record ion currents in cultured TG neurons of rats. Results revealed that 0.5 micromol/L PDBu reduced the amplitude of I(Na)-total by (38.3+/-4.5)% (n=6, P<0.05), but neither the G-V curve (control: V (0.5)=-17.1+/-4.3 mV, k=7.4+/-1.3; PDBu: V (0.5)=-15.9+/-5.9 mV, k=5.9+/-1.4; n=6, P>0.05) nor the inactivation rate constant (control: 3.6+/-0.9 ms; PDBu: 3.6+/-0.8 ms; n=6, P>0.05) was altered. 0.5 micromol/L PDBu could significantly increase the amplitude of I(Na)-TTXr by (37.2+/-3.2)% (n=9, P<0.05) without affecting the G-V curve (control: V (0.5)=-14.7+/-6.0 mV, k=6.9+/- 1.4; PDBu: V (0.5)=-11.1+/-5.3 mV, k=8.1+/-1.5; n=5, P>0.05) or the inactivation rate constant (control: 4.6+/-0.6 ms; PDBu: 4.2+/-0.5 ms; n=5, P>0.05). 0.5 mumol/L PDBu inhibited I(K) by (15.6+/-5.0) % (n=16, P<0.05), and V (0.5) was significantly altered from - 4.7+/-1.4 mV to -7.9 +/-1.8 mV (n=16, P<0.05). I(A) was not significantly affected by PDBu, 0.5 mumol/L PDBu decreased I(A) by only (0.3+/-3.2)% (n=5, P>0.05). It was concluded that PDBu inhibited I(Na)-total but enhanced I(Na)-TTXr, and inhibited I(K) without affecting I(A). These data suggested that the activation of PKC pathway could exert the actions.

Animals ; Female ; Hydrocarbons ; toxicity ; Kidney ; drug effects ; ultrastructure ; Liver ; drug effects ; ultrastructure ; Male ; Mice

Objective To study the changes of the urinary ?-1 microglobulin and urinary immunoglobulin G(IgG) and to investigate the relationship between these two proteins and the allograft function after renal transplantation.Methods Twenty-nine renal transplant recipients were included in the study.Urinary ?-1 microglobulin and urinary IgG were analyzed at d 1,7,14,21,28 after renal transplantation.The allograft function was evaluated based on the clinical manifestations,laboratory and imaging examinations,and the relationship between urinary ?-1 microglobulin,IgG and serum cretinine(SCr) were analysed. Results Urinary ?-1 microglobulin and urinary IgG correlated with SCr after renal transplantation in one month.Of all the 29 cases,14 experienced allograft function recovery(group A),and 15 failed(group B).Urinary ?-1 microglobulin decreased significantly in group A(P

AIM: To explore the feasibility of culturing human umbilical vein endothelial cells ( HUVEC ) on acellular corneal stroma and performing the posterior lamellar endothelial keratoplasty ( PLEK ) treating corneal endothelial decompensation.
METHODS: Thirty New-Zealand rabbits were divided into three groups randomly, 10 rabbits for experimental group, 10 for stroma group and 10 for control group. Corneal endothelial cells were removed to establish animal model of corneal endothelial failure. PLEK was performed on the rabbits of experimental group and stroma group, and nothing was transplantated onto the rabbits of control group with the deep layer excised only. Postoperative observation was taken for 3mo. The degree of corneal edema and central corneal thickness were recorded for statistical analysis.
RESULTS:Corneas in experimental group were relieved in edema obviously compared with that in stroma group and the control group, and showed increased transparency 7d after the operation. The average density of endothelial cells was 2 026. 4±129. 3cells/mm2 , and average central corneal thickness was 505. 2±25. 4μm in experimental group, while 1 535. 6±114. 5μm in stroma group and 1 493. 5±70. 2μm in control group 3mo after operation.
CONCLUSION:We achieved preliminary success in our study that culturing HUVEC on acellular corneal stroma and performing PLEK for corneal endothelial decompensation. HUVEC transplanted could survive in vivo, and have normal biological function of keeping cornea transparent. This study provides a new idea and a new way clinically for the treatment of corneal endothelial diseases.

With the development of organ transplantation for nearly half a century, currently the hot issues in clinical renal transplantation have already shifted to the application of low toxicity immunosuppressive drugs and their projects, as well as the research of technique which induces low or no immunity response to the specific donor. At present the main effective strategies of inducing immune tolerance are donor specific blood transfusion and donor specific bone marrow transplantation. This article summarizes the background, mechanism and clinical application of these two strategies.

The major goal of microbial ecology is to study the structure and function of complex micro-bial communities. New molecular biological techniques have been successfully applied to analyze mi-crobial community structure. However they do not provide information on the physiologic properties of the detected microorganisms. A new tool for structure-function analyses in microbial ecology, micro-autoradiography combined with fluorescence in situ hybridization (MAR-FISH) can be used to simul-taneously examine the phylogenetic identity and the specific activity of microorganisms within a com-plex microbial community at a single-cell level. This article reviews the principle, experimental steps of MAR-FISH technique. The application of this technique in study of the environmental microbial com-munity and function is also summarized.

Objective To assess and compare the Beck Depression Inventory-II ( BDI-Ⅱ) , Hospital Anxiety and Depression Scale-Depression Subscale ( HADS-D) and Center for Epidemiologic Studies Depression Scale ( CES-D) as screening instruments for depression in patients with epilepsy .Methods One hundred and seventeen patients diagnosed with epilepsy were evaluated by BDI-Ⅱ, HADS-D and CES-D, the performance of BDI-Ⅱ, HADS-D and CES-D was evaluated by ROC curve .Results There were 33 epileptics with depression .The sensitivity and specificity of BDI-Ⅱfor the diagnosis of depression was around 90% for critical value 16, the sensitivity of CES-D was more than 80%for critical value 15, and specificity was 72.6%.The sensitivity and specificity of HADS-D was more than 80%for critical value 9, and the sensitivity of HADS-D was 91.3% for critical value 7, and specificity was 76.8%.Three instruments showed a negative predictive value of over 90%.Comparisons of the areas under the ROC curve for these instrument were not statistically significant difference (all P>0.05).Conclusion HADS-D is a brief efficient screening instruments to identify depression in patients with epilepsy .