1.Multiplex RT-PCR assay for the detection of 8 kinds of respiratory viruses
De WU ; Yanling MO ; Lirong ZOU ; Hui LI ; Qiuxia CHEN ; Ling FANG ; Ping HUANG ; Changwen KE
Chinese Journal of Laboratory Medicine 2009;32(8):861-865
. Conclusions Two multiplex RT-PCR assays show high consistency with common RT-PCR. The multiplex RT-PCR assays were initially established.
2.Short-term outcome of laparoscopic adjustable gastric banding for morbid obesity complicated with type 2 diabetes
Yiping ZOU ; Weimin LI ; Hui HUANG ; Fang ZHENG ; Hucheng LI ; Jidong DU ; Haorun LIU ; Ruiguan WANG
Chinese Journal of Digestive Surgery 2008;7(3):171-173
Objective To investigate the short-term outcome of laparoscopic adjustable gastric banding (LAGB) for morbid obesity complicated with type 2 diabetes. Methods Eight morbidly obese patients with type 2 diabetes underwent LAGB from October 2006 to August 2007. The weight parameters, fasting (FBG) and 2-hour blood glucose (2hBG), medication for diabetes were assessed 1,3, 6 and 9 months after surgery. Results All of the patients lost weight, with a mean body mass index decreased from (38.7±7.5) kg/m2 before LAGB to (30.5±4.3) kg/m2 9 months after LAGB. The FBG and 2hBG were decreased significantly at month 6 and 9 after LAGB, with normal FBG and 2hBG in 4 patients. At month 9 after LAGB, 3 of 5 patients with insulin treatment before LAGB were changed to oral hypoglycemics, 1 was continuously administered with a reduced dose of insulin, and 4 patients stopped any medication. Conclusion LAGB is an effective procedure in the treatment of morbid obesity complicated with type 2 diabetes with a favorable short-term outcome.
3.Role of NRF2 signaling pathway in trichloromethane-induced oxidative stress in L02 cells
TAN Shi ying ZHONG Yuan fang QIU Yi bing ZOU Zhi hui YU ri an
China Occupational Medicine 2022;49(05):530-
Objective - ( )
To investigate the effects of nuclear factor erythroid 2 related factor 2 NRF2 on the oxidative stress
( ) Methods ) ,,
induced by trichloromethane TCM in human normal hepatocyte L02 cells. i L02 cells were stimulated with 1 2
, , , ( ),
4 8 12 16 and 20 mmol/L TCM solution dissolved in dimethyl sulfoxide and the control group and blank group were set
, - ,
up. After culturing for 24 hours the cell viability was detected by CCK 8 colorimetric method and the concentration of TCM
) -, -
stimulation was screened. ii L02 cells in logarithmic growth phase were randomly divided into control group and low medium
- , , ,
and high dose groups. After 24 hours of exposure to 0 4 8 and 12 mmol/L TCM the cells were collected. The activity of
( ), ( ), ( - ) ( )
superoxide dismutase SOD catalase CAT glutathione peroxidase GSH Px and the level of malondialdehyde MDA
NRF2, - (HO-1),
were detected by colorimetric analysis. The mRNA expression levels of heme oxygenase 1 glutamate cysteine
(GCLC) () (NQO1) -
ligase catalytic subunit and NAD P H quinone dehydrogenase 1 were detected by real time fluorescence
, - ,
polymerase chain reaction. The protein levels of NRF2 HO 1 GCLC and NQO1 were detected by Western blotting.Results ) , , , ,
i When the concentration of TCM was 4 8 12 16 and 20 mmol/L the survival rate of L02 cells decreased
( P ) , ,
significantly compared with the control group all <0.05 . The concentration of 0 4 8 and 12 mmol/L were selected as the
) , -
stimulation doses for subsequent experiments. ii Compared with the control group the activities of SOD and GSH Px in L02
( P ) ( P ), -
cells in the three doses groups decreased all <0.05 and the levels of MAD increased all <0.05 with a dose effect
- (P ),
relationship. The CAT activity of L02 cells in the medium dose group was lower than that in the control group <0.05 and the
- ( P )
CAT activity of L02 cells in the high dose group was lower than that in the others three groups all <0.05 . Compared with the
, NRF2 - (P ),NRF2
control group the relative expression levels of mRNA in L02 cells in the low dose group decreased <0.05
- (P ), NRF2
mRNA in L02 cells in the medium dose group increased <0.05 mRNA and NRF2 protein expression in L02 cells in
( P ) HO-1,GCLC, NQO1 ,
the highdose group increased both <0.05 . The relative expression level of mRNA and GCLC NQO1
( P )
protein expression in L02 cells in the three doses groups increased compared with the control group all <0.05 . The relative
NRF2 - - -
expression level of mRNA in L02 cells in the high dose group was higher than that in the low and medium dose groups
( P ), - (P ),
both <0.05 and the relative expression of NRF2 protein was higher than that in the low dose group <0.05 but the
HO-1 GCLC - - (
relative expression levels of and mRNA and HO 1 protein level were lower than those in the medium dose group all
P )Conclusion -
<0.05 . TCM exposure can inhibit the proliferation of L02 cells by inducing oxidative stress with a dose effect
,
relationship. In this process the antioxidant mechanism mediated by NRF2 was activated. The expression of antioxidant defense
, - ,
and detoxification related target genes downstream of NRF2 signaling pathway was activated and the expression of HO 1
-
GCLC and NQO1 was up regulated to alleviate the oxidative damage caused by TCM.
4.Study on female sexual dysfunction in type 2 diabetic Chinese women.
Yao Fang SHI ; Xin Yu SHAO ; Qing Qing LOU ; Ya Juan CHEN ; Hui Juan ZHOU ; Jian Ying ZOU
Biomedical and Environmental Sciences 2012;25(5):557-561
OBJECTIVETo investigate the female sexual dysfunction (FSD) in type 2 diabetes patients, by comparing the sexual function between type 2 diabetic women and non-diabetic women with Female Sexual Function Index (FSFI).
METHODS115 type 2 diabetic women and 107 age-matched non-diabetes women were enrolled with similar backgrounds. Their sexual functions were evaluated with FSFI. Metabolic parameters such as body mass index, blood lipid profile, hemoglobin A1C, plasma glucose were also collected.
RESULTSTotal score of FSFI of the type 2 diabetic women were significantly lower than that of the non-diabetic controls (18.27±8.96 vs. 23.02±5.78, P=0.000). Scores of the FSFI domains (desire, arousal, lubrication, orgasm, satisfaction, pain) of the type 2 diabetic group were also lower than those of the control group. According to the FSD criterion (FSFI<25) available in China, the percentage of FSD in the type 2 diabetic group was significantly higher than that of the control group (79.2%vs. 55.0%, P<0.001). These trends seemed more prominent in pre-menopause subgroups. The logistic regression analysis indicated that age and diabetes were independent risk factors of FSD. Body Mass Index (BMI) also had influence in the diabetes group.
CONCLUSIONFindings from this study showed that there are more FDS in Chinese type 2 diabetic women than in their non-diabetic counterparts, especially in pre-menopause participants.
Adult ; Asian Continental Ancestry Group ; Diabetes Mellitus, Type 2 ; complications ; Female ; Humans ; Middle Aged ; Sexual Dysfunction, Physiological ; etiology
5.Laparoscopic adjustable gastric banding for morbid obesity
Yiping ZOU ; Hui HUANG ; Weimin LI ; Fang ZHENG ; Hucheng LI ; Jidong DU ; Haorun LIU ; Ruiguang WANG ; Zhaotao JIANG
Chinese Journal of General Surgery 2009;24(4):313-316
Objective To evaluate the effect of laparoscopic adjustable gastric banding(LAGB) in patients with obesity and obesity-related comorbidities.Methods From Oct.2006 to Dec.2007,50 morbidly obese patients including 11 cases with type 2 diabetes,3 with hypertension,15 with hyperlipidemia,28 with fatty liver,1 with obstructive sleep-apnea syndrome and 2 cases with gallstones underwent LAGB.The mean follow-up period for these patients was 11.2 months.ranging from 6 to 18 months.The weight loss,obesity-related comorbidities,outcomes and complications were evaluated.Results Mean BMI decreased significantly from preoperative(39±6)kg/m2 to postoperative(31±4)kg/m2,(28±7)kg/m2 and(27±7)kg/m2 respectively at 9,12 and 18 months(P<0.05).The mean excess weight loss at 9,12 and 18 months postoperatively was 30%±11%、42%±13%and 45%±13% respectively.At 12 and 18 months,respectively,20%and 44%of patients had>50%excess weight loss.The obesity-related comorbidities resolved or improved in 66%~100%of the patients at 12 and 18 months postoperatively.Complications occurred in 4 cases,among them 3 cases were cured conservatively and in 1 case reoperation was performed. Conclusions Based on short-term follow-up results,LAGB is a safe,effective and feasible technique in the treatment of patients with morbid obesity and obesity-related comorbidities.
6.Characteristics of neural information encoding in epileptic networks involved in rat caudate putamen-hippocampi.
Li GAN ; Qing LIU ; Hui-Lang LIU ; Fang-Ming DIAO ; Zu-Yu ZOU ; Dan HAN
Chinese Journal of Applied Physiology 2006;22(2):179-184
AIMTo study the characteristics of neural information encoding of the epileptic networks involved caudate-putamen(CPu) and the hippocampi induced by tetanization of the right CPu in rats.
METHODSExperiments were performed on 59 SD rats. Acute or chronic tetanization of the right CPu (ATRC or CTRC) (60Hz,0.4-0.6 mA, 2 s) was used to induce rat epilepsy.
RESULTS(1) The bursting or primary unit afterdischarges of single neurons were asymmetric in dual hippocampi induced by the ATRC. (2) Continuous sharp waves were observed in ipsilateral or contralateral CPu induced by the CTRC. The oscillatory network seizures with phase shift appeared between two sharp waves in ipsilateral CPu. The frequency of oscillatory waves was negatively correlated with the time and fluctuated from 70 Hz to 110 Hz, then to 35 Hz, and finally to 30 Hz. (3) In the contralateral side primary network after discharges in the CPu were induced by the CTRC. Therefore, the characteristic primary network afterdischarges could be shifted from the CPu or to the HPC, but amplified. On the other hand, HPC sharp waves could be depressed when the CPu network seizures occurred.
CONCLUSIONThe reestablishment of CPu-hippocampal epileptic networks could be transhemispherically promoted by over-activation of the right CPu network, in which bilateral hippocampal neuronal network and CPu neural network were involved in some particular pathophysiological information encoding.
Animals ; Caudate Nucleus ; metabolism ; Epilepsy ; physiopathology ; Hippocampus ; metabolism ; Male ; Neurons ; metabolism ; Putamen ; metabolism ; Rats ; Rats, Sprague-Dawley
7.Alteration of expression of survivin in HL-60 cells treated with chemotherapeutic drugs.
Yao-Hui WU ; Ping ZOU ; Fang LIU ; Ming ZHANG ; Jian-Hua CHENG
Journal of Experimental Hematology 2006;14(2):347-350
Survivin, a new member of the inhibitor of apoptosis protein (IAP) family, expressed in the most human cancers but not in terminally differentiated adult tissues, so survivin may be a target for tumor therapy. In addition, some scholars found that survivin expression is associated with the resistance in chemotherapy. To explore the relationship between survivin and drug-resistance, the alteration of survivin mRNA and protein of HL-60 cells treated with daunomycin (DNR), mitoxantrone (MIT) and arsenic trioxide (As2O3) was investigated, the expressions of survivin mRNA and survivin protein were detected on the first and third day by RT-PCR and Western blot, respectively. The results showed that survivin mRNA levels all decreased after the first day of treatment with drugs. It was decreased by 10% in DNR group, 40% (P < 0.01) in MIT group, and 25% (P < 0.01) in As2O3 group in comparison with control cells. In the third day, the survivin mRNA treated with DNR was up-regulated by 20% (P < 0.05), compared with the first day, and MIT was up-regulated by 65% (P < 0.01), but As2O3 was still down-regulated by 32% (P < 0.01). In Western blot, survivin protein level increased 14% after treated with DNR for three days, compared with the control cells, and 11% in MIT, but decreased by 82% in As2O3. It is concluded that after treatment with chemotherapeutic drugs, the survivin level descended at first day and then ascended obviously. This phenomenon may be associated with the resistance in chemotherapy for leukemia. On the other hand, As2O3 shows a different mechanism that may play a significant role to reverse resistance.
Antineoplastic Agents
;
pharmacology
;
Arsenicals
;
pharmacology
;
Daunorubicin
;
pharmacology
;
Drug Resistance, Neoplasm
;
drug effects
;
HL-60 Cells
;
Humans
;
Inhibitor of Apoptosis Proteins
;
Microtubule-Associated Proteins
;
biosynthesis
;
genetics
;
Mitoxantrone
;
pharmacology
;
Neoplasm Proteins
;
biosynthesis
;
genetics
;
Oxides
;
pharmacology
;
RNA, Messenger
;
biosynthesis
;
genetics
8.Expression of survivin in leukemia cells and influence on it by GM-CSF.
Yao-Hui WU ; Ping ZOU ; Fang LIU ; Han-Bing SHEN
Journal of Experimental Hematology 2007;15(1):6-9
This study was aimed to explore the expression of survivin in leukemia cells and to investigate the effect of GM-CSF on survivin expression. The survivin expressions in 37 previously untreated leukemia patients and 10 normal persons as well as in three kinds of leukemia cell lines (K562, HL-60, U937) were analyzed by RT-PCR. The HL-60 cells were treated by the proper concentration of GM-CSF, and the changes of survivin mRNA and protein expression levels were detected by using RT-PCR and Western blot respectively. The results indicated that the positive rate of survivin gene in 37 leukemia patients was 67.6% (25/37) and significantly higher than that in normal control (20.0%). The expression level was higher in ALL cells than that in AML cells (73.3% vs 63.6%). Moreover, three kinds of leukemia cell lines all expressed survivin. After treating with the proper concentration of GM-CSF for 2 days, the expression of survivin obviously increased and the expression level of survivin mRNA was up-regulated by 26%, the expression level of survivin protein was up-regulated by 49%. It is concluded that the survivin gene extensively express in leukemia and its cell lines, and its expression can be obviously increased by GM-CSF.
Adolescent
;
Adult
;
Aged
;
Female
;
Granulocyte-Macrophage Colony-Stimulating Factor
;
pharmacology
;
HL-60 Cells
;
Humans
;
Inhibitor of Apoptosis Proteins
;
K562 Cells
;
Leukemia, Myeloid, Acute
;
metabolism
;
Male
;
Microtubule-Associated Proteins
;
biosynthesis
;
genetics
;
Middle Aged
;
Neoplasm Proteins
;
biosynthesis
;
genetics
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
metabolism
;
U937 Cells
9.Mechanism of laparoscopic adjustable gastric banding in the treatment of obesity with type 2 diabetes mellitus.
Zhao-tao JIANG ; Yi-ping ZOU ; Hui HUANG ; Fang ZHENG ; Xin DAI ; Ya LI
Chinese Journal of Gastrointestinal Surgery 2010;13(7):520-523
OBJECTIVETo explore the mechanism of laparoscopic adjustable gastric banding (LAGB) in the treatment of obese patients with type 2 diabetes mellitus (T2DM).
METHODSA total of 20 patients with obesity and T2DM were treated with LAGB. During the postoperative 1, 3, 6, 9, 12 months, the body weight changes were monitored and body mass indices (BMI) were calculated. The serum levels of leptin, GLP-1, and ghrelin were examined preoperatively and 1, 3, 6, 9, 12 months after LAGB using enzyme-linked-immunosorbent assay (ELISA). At the same time, the fasting serum insulin (FINS), C-peptide, glycated hemoglobin (HbA1c) levels were examined by electrochemiluminescence and the level of fasting blood glucose (FBG) was tested with oxidase test.
RESULTSAt postoperatively 12 months, all the 20 patients lost weight. The mean body weight decreased from (108 + or - 18) kg to (71 + or - 16) kg (P<0.05) and BMI decreased from 38 + or - 5 to 29 + or - 6 (P<0.05). The HOMA-IR decreased from (12.8 + or - 7.4) to (3.4 + or - 2.0) (P<0.01). The serum ghrelin level increased from (7.8 + or - 1.9) microg/L to (11.6 + or - 2.6) microg/L (P<0.01). The serum leptin level declined from (24.9 + or - 13.7) microg/L to(12.9 + or - 5.1) microg/L (P<0.01). The serum GLP-1 level increased from (0.58 + or - 0.12) microg/L to(0.80 + or - 0.06) microg/L (P<0.01). After LAGB, there were positive correlations between serum leptin level and FBG, FINS, HbA1c,and C-peptide level. Serum ghrelin and GLP-1 were negatively correlated with FBG, FINS, HbA1c,C-peptide.
CONCLUSIONSLAGB is effective in treatment of obesity patients with T2DM. The mechanism may be associated with the increase of serum GLP-1 and ghrelin and the decrease of serum leptin and insulin resistance.
Adult ; Blood Glucose ; Body Mass Index ; Diabetes Mellitus, Type 2 ; complications ; surgery ; Female ; Gastroplasty ; methods ; Ghrelin ; metabolism ; Glucagon-Like Peptide 1 ; metabolism ; Glycated Hemoglobin A ; metabolism ; Humans ; Laparoscopy ; Leptin ; metabolism ; Male ; Middle Aged ; Obesity ; complications ; surgery ; Young Adult
10.Analysis of clinical characteristics of 43 surgical patients with Crohn disease using the Montreal classification.
Xue-feng LI ; Fang-gen LU ; Yi-you ZOU ; Chun-hui OUYANG ; Ling-juan YE ; Zheng-gen WANG
Chinese Journal of Gastrointestinal Surgery 2010;13(3):210-212
OBJECTIVETo investigate the clinical features of Crohn disease according to the Montreal classification.
METHODSClinical data of 43 surgical patients with Crohn disease (surgical group) and 125 non-surgical patients with Crohn disease (non-surgical group) were retrospectively analyzed and compared between two groups. The Montreal classification was used.
RESULTSIn the surgical group, 28 patients (65.1%) were A2, 14 (32.6%) were A3 and only one was A1, which was not significantly different as compared to the non-surgery group. The proportions of L1, L2, L3, and L4 subtype in the surgical group were 41.9%, 25.6%, 30.2%, and 2.3%, respectively, which was not significantly different as compared to that in the non-surgery group. In the surgical group,B1 disease was found in 1 case (2.3%), B2 in 26 cases (60.5%), and B3 in 16 cases (37.2%), while in the non-surgical group, B1 was found in 79 cases (63.2%), B2 in 44 cases (35.2%) and B3 in 2 cases (1.6%). Differences were significant between two groups in disease behavior (P=0.001, P=0.004, P=0.001).
CONCLUSIONSMost surgical patients of Crohn disease are A2. L1 and L3 are the main lesion location. As disease behavior, B2 and B3 are the main reasons for operation.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Crohn Disease ; classification ; pathology ; surgery ; Female ; Humans ; Male ; Middle Aged ; Reference Standards ; Retrospective Studies ; Young Adult