Animals ; Curcumin ; pharmacology ; therapeutic use ; Humans ; Liver Cirrhosis ; drug therapy

Objective To evaluate mid-term clinical and radiographic outcomes associated with cementless total hip arthroplasty(THA) in a consecutive series of patients with osteonecrosis of the femoral head. Methods This study examined the mid-term (equal to or more than 5 years) results of THA. From January 1998 to March 2001, a series of 71 patients (80 hips) who had undergone THA with cementless components in our hospital were involved in this study. All patients were evaluated both clinically and radiographically. Clinical outcomes were evaluated according to Harris evaluation score. Components migration, bone changes around the implant, the polyethylene wear rate and direction of the acetabular polyethylene wear were measured radiologically. Kaplan-Meier analysis was performed to evaluate the survival of the femoral and acetabulum components. End point was radiographical loosening or revision of the acetabular component, and revision of the femoral component for any reason. Results 62 hips in 54 patients were followed up at least 5 years. The mean Harris hip score improved from preoperation (44.0?8.4) points to (92.4?5.7) points at the time of final follow-up. No component was seen to be loose radiographically or had been revised because of any reason at the time of final follow-up. Only one focal area of pelvic osteolysis in one patient and twelve small focal areas of femoral osteolysis in another patient were identified. The mean linear wear rate was (0.125?0.074) mm/year. When loosening was used as the end point for failure, the survival rate of the acetabular and femoral components was 1.0 (95% confidence interval, 0.98 to 1.00) in both groups. Conclusion Cementless THA in patients with osteonecrosis of the hip had a satisfactory clinical and radiographic outcome at a minimum of five years follow-up. Because polyethylene wear and osteolysis can not avoided, radiographical follow-up for cementless components is very important.

Objective To summary the color Doppler imaging feature of localized Castleman disease.Methods From January 1997 to November 2011,32 localized Castleman diseases which were proved by pathology were analyzed.Results Round-like,hypoechoic,hypervascular lesions were showed in 23 hyalinevascular type lesions,2 of them with calcium,3 of them with structure liked lymph node hilum.Round-like,hypoechoic,hypervascular lesions or normal lymph node were showed in 5 plasma- type lesions and 4 mixed type lesions.Conclusions When round like,hypoechoic,hypervascular lesion is found by ultrasonography,Castleman should be considered.Calcium or lymph nod hilum-like structure is special finding in diagnosis of Castleman disease by ultrasonography.

In order to investigate the therapeutic effect of traditional chinese medicine "Re Du Qing" on silicosis, the cultured and purifiel peritoneal macrophages in five groups obtained from mice were observed dynamically with cytochemical me- thods and scanning electron microscope. The results showed that the survival times of cell cultures were 2-3 weeks, 24-48, 72, 48-72 and 48-72 hr in normal, silica, "Re Du Qing", P204 and joint action group respectively in vitro. The characteristics of cell morphology with a series of cell surface ultrastructural changes in different times of culturd and the stages of various function were different in five groups. The cell surface ultrastructural changes of "Re Du Qing" group were similar to the normal group. The macrophages in the silica group phagocytosed silica dusts rapidly died and broken down much earlier than the cells in "Re Du Qing" group. The cell surface ultrastructural changes in P204 group were less than that of the cells in silica group, whereas the eell surface ultrastructural changes in joint action group were between the cells in "Re Du Qing" and P204 groups. The activities of intracellular acid phosphatase (AcP) and succinic dehydrogenase (SDH) in silica group were also much lower than that of "Re Du Qing" one. This study suggested that traditional chinese medicine "Re Du Qing" is evidently more effective on therapy of experimental silicosis.

Objective To provide structure and function applied anatomical of auricle stabilizing system for plastic and reconstruction.Methods Twelve cases fresh ears from adult head specimen,to dissect the skin,muscle fascia system and cartilage,to observe nerve,blood vessel and structure of cartilage.Results The structure of auricle Including the perichondrium,auricle cartilage,muscle and ligament.There was a rich blood supply for auricle stabilize system.The mainly blood supply come from posterior auricular artery diameter was 1.2 ± 0.2 cm and superficial temporal artery diameter was 2.6 ± 0.3 mm.Vein accompanying with the same name.Lymphatic flow in the area of superficial muscle fascia.Conclusion The blood supply of posterior auricular artery and superficial temporal artery play a crucial role in auricle repair and reconstruction.The main structure of stable system was elastic cartilage.Auricle cartilage and muscle play an important role in the microsurgery of auricle cartilage morphology and location.

AIM: To study the effect of double gene transduction mediated by lentiviral vectors on the characteristics of human embryonic stem cells (hESCs). METHODS: Using the backbone from inducibal dual and excisable transgene vector (iDuet101) , lentiviral vectors overexpressing cytotoxic T lymphocyte - associated molecule - 4 immuno-globulin (iDuet101 - CTLA4Ig) , indoleamine 2, 3 dioxygenase (iDuet101 - IDO) , and ubiquitin - C promoter - lueifer-ase - ires - puromycin ( ULIP) were constructed and packaged according to the standard recombinant techniques. Human embryonic stem cells (hESCs) were first transduced with iDuet101, iDuet101 - CTLA4Ig or iDuet101 - IDO, then, after the selection, were transduced again with ULIP. The expression and function of the exogenous genes were detected. Immu-nohistochemistry, RT - PCR and flow cytometry were applied for detection of embryoid bodies ( EB) formation in vitro and in vivo teratoma formation. RESULTS: Double - transduced hESCs showed typical shape of cell clones and positive staining of tumor rejection antigen -1 - 60 ( Tra -1 - 60 ) and octomer transcription factor - 4 ( OCT - 4 ). The formation of EB was observed, in which a - fetoprotein (AFP), paired box gene 6 ( Pax6) and Musashi 1 ( MSI1) were positively expressed. The cells formed teratomas, and the luciferase signals existed until 28 days after xeno - transplantation. CONCLUSION : Double transduction of non - transcriptional factors mediated by lentiviral vectors does not affect the cell growth rate and their differentiation ability.

Adult ; Altitude ; Arteries ; metabolism ; Calcitonin Gene-Related Peptide ; blood ; Humans ; beta-Endorphin ; blood

0.05). Conclusions Our study does not support a positive association between Chlamydia pneumoniae infection or reinfection and MS. Chlamydia pneumoniae may be a bystander pathogen.

Objective Purpose This prospective randomized study was to explore the efficacy of different methods in preventing postoperative air leakage of video-assisted thoracoscopic (VATS) bullectomy for primary spontaneous pneumothorax.Methods The patients who underwent VATS bullectomy from January 2015 to March 2016 in Beijing Chao Yang Hospital were enrolled,and they were randomly assigned to experimental group and control group.The experimental group applied an absorbable polyglycolic acid(PGA) sleeve combining with an automatic stapler in the bullectomy.The control group resected the bullae using the automatic stapler alone.Covering the staple line with an absorbable polyglycolic acid sheet and pleural abrasion were performed in both groups.The baseline characteristics of the patients,postoperative air leakage,drainage tube removing time,postoperative hospital-stay,and postoperative complication were recorded.Results Finally,123 patients were enrolled in the study,the experimental group and control group were 58 and 65 cases,respectively.There was no operative mortality in either group.Compared with the control group,the postoperative air leakage (0.53 ± 0.99) days,chest tube drainage (2.98 ±1.03) days,postoperative hospital-stay (3.88-± 0.91) days in the experimental group were all significantly shorter.The rate of postoperative complications in the experimental group was(3.4％),of which one patient catched a pulmonary infection,the other one postoperative atelectasis.However,complications were developed in ten patients (15.4％) in the control group,which was significantly higher compared with the experimental group,including pulmonary infection five cases,postoperative atelectasis three cases,pleural effusion two cases.Conclusion The appliance of PGA sleeve in the operation for primary spontaneous pneumothorax could effectively prevent postoperative air leakage,as well as reduce the postoperative drainage tube removing time.Furthermore,the patient could recover more soon from the surgery,and have a shorter hospital-stay.