Objective To explore the therapeutic mechanism of Xiao Chaihu Decoction for endometriosis in rats. Methods The rat models of endometriosis were treated with Xiao Chaihu Decoction, Danazol, and Xiao Chaihu Decoction plus Danazol for 4 weeks,respectively. And then cyclooxygenase-2(COX-2) expression in endometrium and endometriotic tissue was observed by immunohistochemistry method. Results The expression of COX-2 in the endometriotic tissue was lower than that in endometrium in Xiao Chaihu Decoction group and Xiao Chaihu Decoction plus Danazol group, while the results in Danazol group and control group were opposed to those in the above two groups. Conclusion The mechanism of Xiao Chaihu Decoction for rats endometriosis may be related to the decrease of COX-2 expression in ectopic Endometrium, thus reducing the formation of prostaglandins.

Adult ; Aged ; Chlorine ; poisoning ; Female ; Follow-Up Studies ; Humans ; Lung ; physiopathology ; Male ; Middle Aged ; Young Adult

Purpose To investigate the correlation between promoter methylation of RKIP and the clinicopathological features of gastric carcinoma and to analyze the correlation between promoter methylation of RKIP and expression of RKIP. Methods The protein ex-pression and promoter methylation of RKIP were detected in 45 cases of surgically resected gastric carcinoma specimens and 21 cases of corresponding normal tissues using the immunohistchemistry SP method and methylation-specific PCR ( MSP) . Results The positive rate of promoter methylation of RKIP was 48. 89% in gastric carcinoma tissues and 4. 8% in adjacent tissues (P<0. 05). The positive rate of the expression of RKIP protein was 42. 22% in gastric carcinoma tissues and 90. 48% in adjacent tissues (P<0. 05). The pro-tein expression and promoter methylation of RKIP were correlated with the degree of differentiation and lymph node metastasis ( P<0. 05) . Expression of RKIP protein in gastric carcinoma was associated with promoter methylation of RKIP. Conclusions Promoter abnormal methylation of RKIP may be one reason of the deletion of expression of RKIP protein, which correlates with occurrence and development of gastric carcinoma.

ObjectiveTo investigate the influence of nateglinide treatment of newly diagnosed patients with type 2 diabetes on the state of inflammatory response. MethodsThe clinical data of 74 newly diagnosed patients with type 2 diabetes were retrospectively reviewed,and treated with nateglinide,before and after treatment,the fasting blood glucose (FBG), postprandial blood glucose 0.5h ( 0.5 hPG), 1 h postprandial blood glucose ( 1 hPG) ,2h postprandial blood glucose(2hPG) ,fasting insulin(FINS) ,0.5h postprandial insulin(0. 5hINS) ,postprandial 1h insulin( 1 bINS),2h postprandial insulin(2hINS),interleulin-2(IL-2) and C-reactive protein(CRP) levels were observed. ResultsAfter treatment,the FBG,0. 5hPG, 1hPG,2hPG, FINS,0. 5hINS, 1hINS,2hINS, IL-2 and CRP of patients were ( 8.0 ± 1.5) mmol/L,(12.0±1.8)mmol/L,(10.2 ± 1.3) mmol/L,(10.5 ±1.2) mmol/L,(168.2 ±11.5) pmol/L,(213.5±23.5) pmol/L,(197.0 ±21.5) pmol/L,(189.5 ±12.0) pmol/L,(14.0 ±1.5) μg/L, (13.5 ±1.5) mg/L,compared with( 10. 5 ± 1.0) mmol/L, ( 14. 5 ± 1.5) mmol/L, ( 12. 5 ± 1.4) mmol/L, ( 11.6 ±2.0) mmol/L,(180.7 ±12.0) pmol/L,(229.8 ±26.0) pmol/L,(218.5 ±23.0) pmol/L, (197.0± 14.5) pmol/L,(12.5 ±2.0) μg/L, (22.8 ±2.0) mg/L before treatment decreased significantly(t =11. 9293,9. 1785,10. 3561,4. 1115,6. 4696,4.0009,5. 8744,3. 4279,5. 2307,32.0006, all P ＜0. 05). There was no serious adverse events in treatment process. ConclusionNateglinide treatment of newly diagnosed patients with type 2 diabetes could significantly improve the patient's inflammatory response state,and there was no serious adverse events in treatment process.

Animals ; Danazol ; pharmacology ; Endometriosis ; drug therapy ; Endometrium ; drug effects ; Female ; Interleukin-6 ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley

A microprocedure for the simultaneous determination of retinol and alpha-tocopherol in 100 ?l human plasma by reversed phase high pressure liquid chromatography without precolumn is detailed in this paper. A solvent system of methanol/water (96:4) provides clear separation of these two fat soluble vitamins. The chromatogram is complete in 12 min. The retinol and al-pha-tocopherol are quantitated by the peak height ratio and weight ratio method using retinyl acetate as an internal standard. The coefficient of variation of ten determinations is less than 5%. The mean recovery of four different concentrations of retinol and alpha-tocopherol are 102.2110.3% and 95.8?9.9% respectively. This microprocedure should be useful for assessment of the nutritional status of these two vitamins and might help to elucidate the relationship between them.

Objective: To express and purify transforming growth factor ?(TGF?)-pseudomonas exotoxin 40 and investigate its cytotoxic effect on cancer cells overexpressing epidermal growth factor (EGF) receptor. Methods: Recombi nant plasmid pV28 was constructed by inserting the gene coding TGF?-PE40 into the vector pET28a Expression of fusion protein was conducted using the host BL21. Production of the recombinant protein was induced by IPTG, following extraction and purification of inclusion bodies with His-tag purification system. Cell viability assay (by MTT) was performed to determine the cytotoxic effect of TGF?-PE40 on cancer cells ( A431 and SK-OV3). Results: Recombinant plasmid pV28, which expresses TGF?-PE40, was constructed successfully. Purity of TGF?-PE40 was about 98% after a purification procedure using His-tag column. Cytotoxic experiment showed that at a concentration of 0. 86?0. 07 UUUUg/ml, TGF?-PE40 could reduce 50% viability of A431, which has high expression of EGFR. Whereas the IC50 for ovarian cancer cell SK OV3, which expresses less EGFR, was 6.37?2.18 ?g/ml. There was a significant difference between these two groups (P

A blood film slide taken from a patient previously diagnosed as vivax malaria in Mojiang County, Yunnan Province, showing atypical forms. The ring forms had multinuclei, and the late trophozoites trended to form band. The schizonts and gametocytes were somewhat alike to Plasmodium vivax. PCR amplification confirmed that the patient was infected by P.knowlesi.

The objective of this retrospective study was to evaluate the myocardial protection of different periop erative procedures in 100 cases with heart-valve replacement (HVR) between 1980 and 1992. Before Feburary. 1989. HVRs of 48 cases (group Ⅰ) were undertaken with hypothermia (28C=30C) and cold cardioplegic solution (4C. 500ml). under morphine-diazepam-pancuronium anesthesia: following cardiac resuscitation, the procedures were performed including blood transfusion, diuresis, supplemental KCI. inotropic support with dopomine, norepincphrine and aramine. and continuous mechnical ventilation. Since 1989, the procedures were increased to be conducted in 52 cases (group Ⅱ) as followed: A. preoperative managements for achiving the optimum status of cardiac function: 1. oxygen therapy by mask. 2. antirhenmatic treatment for patients aged less than 35 years. 3. oral isosorbide and captopril for pulmonary hypertension, 4, infusion with fructose 1.6-diphusphatc and glucose-insulin-KC1 mixture. 5. clinical dose of digitora and diuretics for cardiac failure; B. methods during surgery: 1. anesthesia was induced with fentanyl, midazolam and pancuronium. and maintained with inhalation of nitrous oxide and infusion of fentanyl at 30-35?g/kg/min, 2, hy pothermia (24C-27C) and cold cardioplegic solution(600-1000ml) 3. immediately before the blockage of aorta was overcome. the artificial perfusion blood pressure was reduced; afterwards, it was gradually recovered, in order to prevent myocardium from injury of reperfusion. 4. intra-and extracoporal circulations worked together over half of blokage time of arota, to improve myoardial oxygen delivery; C. treatments in early postoperative stage: 1. continuous positive end-expiratory pressure ventilation, 2. infusion with magnesium-potassium aspartyl: and the others were similar to those before 1989. As compared with those in group 1. the rate of spontanous restoration of beats increased significantly (P

0 05), but fas proteins decreased gradually (P