Child ; Early Diagnosis ; Hand, Foot and Mouth Disease ; diagnosis ; Humans

OBJECTIVE:To provide reference for the rational drug use and glycemic control for patients with type 2 diabetes. METHODS:160 patients with type 2 diabetes in 3 communities of Tianjin were divided into 2 groups based on the understanding of the diabetic knowledge,group A(86 cases)had understanding of diabetic knowledge and group B(74 cases)had no. They were carried on the questionnaire survey about the general information,medication and glycemic control. RESULTS:A total of 160 ques-tionnaires were conducted,and 160 were effective with the effective rate of 100%. The proportion of patients with highly educated (college or above)in group A was significantly higher than group B,low education(secondary or below)was significantly lower than group B(P<0.05). The proportion of patients with medicine background in group A was significantly higher than group B(P<0.05). There were no significant differences in the use ratio of different types of drugs for diabetes between group A and group B (P>0.05),however,the use ratio of self-purchase functional food in group A was 0,and group B(60.8%)was significantly high-er than group A(P<0.05). The recent self-test average blood glucose levels,the average blood glucose levels in the investigation day,the 2 h postprandial blood glucose and glycosylated hemoglobin levels in group A were significantly lower than group B(P<0.05). CONCLUSIONS:The medication and glycemic control of patients with type 2 diabetes is closely related to the understand-ing of glycemic knowledge. Therefore,propaganda and education of medication and related functional food for patients need to be strengthened to promote the rational medication.

Objective To identify the chemical components of traditional Chinese medicine by liquid chromatography-mass spectrometry.Methods Chemical components of 86 traditional Chinese medicines were identified by two methods,observation group with LC-MS method and control group with traditional LC methods.The identification efficacy,accuracy,and identification time between two methods were compared. Results The excellent rate in observation group was 76.74% (66/86),the excellent and good rate was 90.70%(78/86),which were significantly higher than those in control group (P<0.05 ).83 kinds of traditional Chinese medicine were successfully identified in observation group,the success rate was 96.5 1%,significantly higher than control group(P<0.05).The identification tine,retention time after correction of errors in observation group were significantly less than control group(P<0.05 ).Conclusion LC-MS has high accuracy,short identification time and wide adaptation range in identification of chemical components of traditional Chinese medicine.

Due to practical and ethical concerns associated with human experiments, animal models have been essential in cancer research.Vast resources are expended during the development of new cancer therapeutics, and selection of optimal in vivo models should improve this process.Genetically engineered mouse models ( GEMM) of cancer have progressively improved in technical sophistication and, accurately recapitulating the human cognate condition, have provided opportunities to accelerate the development of cancer drugs.In this article we consider the different types of animal models used for predicting the results of clinical trials of cancer drugs, and discuss the strengths and weaknesses of each in this regard.In addition, the methods of predicting in vivo models and clinical translation are discussed.

Objective:To develop a method for the simultaneous determination of vinyl chloride and vinylidenechloride in drug packaging materials containing polyvinyl chloride /polyvinylidene chloride by HS-GC.Methods: A Stabilwax (30.0 m ×0.25 mm, 0.25 μm) capillary column and an FID detector were adopted .The headspace equivalent temperature was 80℃for 30 min and the col-umn temperature was 40℃.The inlet temperature was 190℃and the detector temperature was 210℃.N2 was used as the carrier gas . The flow rate was 1.0 ml · min-1 .Results: Vinyl chloride had a good linear relationship within the range of 0.5-2.5 μg ( r =0.996 7), and the average recovery was 90.4%(RSD=0.9%, n=9).Vinylidenechloride had a good linear relationship within the range of 1.0-5.0 μg (r=0.999 0), and the average recovery was 90.0%(RSD=0.6%, n=9).Conclusion: The method is fast and accurate in the simultaneous determination of vinyl chloride and vinylidenechloride in drug packaging materials containing polyvinyl chloride/polyvinylidene chloride .

The connotation development of industry and education is the road to advance the development of connotation and the quality of higher vocational and technical education.Based on this and bringing into play the advantages of running a vocational school and combined with cultivating the applied personnel needed by enterprises and professions,the college perfects the personnel cultivation model.carries out the "225" education model,accelerate the development of the teacher,promote its service capacity to enhance constantly the quality of higher vocational and technical education.

BACKGROUND: The parameters of blood platelet(B-PLT) can reflect its thrombo-function, but the variation of blood-platelet parameters in patients with thrombotic disease is controversial and not unanimous.OBJECTIVE: To observe the changing rule of the B-PLT parameters in patients with cerebral infarction(CI), and investigate whether blood cell counter can be used in the evaluation of the variation of B-PLT parameters in CI patients.DESIGN: A case-control and experimental study based on patients and healthy people.SETTING: Department of laboratory and internal medicine in a university.PARTICIPANTS: During the January and March 2002, 37 CI patients including 28 males and 9 females aged 45 - 87 years were selected from Department of Neurology, Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology. Among 37 healthy people,20 males and 17 females aged 40 - 60 years were selected as control group.METHODS: Platelet distribution width (PDW), mean platelet volume (MPV), platelet-large cell ratio(P-LCR) and the changing quantity of B-PLT of 37 CI patients were analyzed with blood cell counter, meanwhile,the healthy people were analyzed as control subjects.MAIN OUTCOME MEASURES: Changes of B-PLT parameters in two groups respectively.RESULTS: A positive relation between PDW and P-LCR was observed and between P-LCR and MPV as well( r = 0. 99, 0.92, P ＜ 0. 001 ). A negative correlation between B-PLT quantity and MPV was observed and also between B-PLTquantity and P-LCR(r= -0.58, -0.59, P ＜0.05) .In CI patients, PDW, MPV and P-LCR were(14.51 ± 2. 88)%, (10.95 ± 1.48) fl and(34.24 ± 11.23)% respectively, which were higher than those of healthy people [ (12. 86 ± 2.02)%, (10. 19 ± 1.29) fl, (28.47 ± 9.41 )% ]respectively, and the difference was significant( P =0. 019 31, 0. 021 77 and 0.026 35), but there was no significant difference between the two groups in B-PLT quantity.CONCLUSION: The blood platelets' parameters in CI patients, which reflect the function of the B-PLT, are significantly higher than those in healthy people. It shows that the enhanced function of platelet is a risk factor of cerebral infarction, the use of blood cell counter in analysis can observe the variation of the platelets' function rapidly and accurately. It can be a useful method of the therapy and prevention in CI patients.

Chickenpox is an acute and highly infectious respiratory disease commonly seen in children.Varicela－zoster often results from the activation of latent virus in chickenpox called varicella－zoster virus（VZV）or called human herpesvirus type 3. Primary infection of VZV may induce typical disease of various severity and most healthy children infected with VZV have mild symptoms and good prognosis.But VZV infection，if occurs in special population such as persons of immune inhibition treatment will induce serious consequence or even death and it is as same serious as in adults.A detailed illustration of molecular biology，epidemiology，laboratory diagnosis，treatment and preventive strategy of the VZV were given.

OBJECTIVE: To discuss the biosafety and biocompatibility of biomaterials. METHODS: A computer-based online search of CNKI, CBM and VIP as well as manual search were performed to collect articles about random cell control experiments and animal experiments of biological material biocompatibility published between 1990 and 2008. A total of 28 Chinese literatures were collected, and 7 were included mainly involving the cytotoxicity test methods and blood compatibility of the experimental medium, experimental grouping, experimental materials, methods of observation, experimental results, and experimental conclusion. In addition, biocompatibility of the biological material was analyzed to summarize the biocompatibility of the biological safety. RESULTS: Based on experiments of biosafety and biocompatibility of biomaterials, the cytotoxicity test and blood compatibility of various biomaterials, such as collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, are essential for the biological safety. The experimental results have shown that the biomaterials have good biocompatibility. CONCLUSION: A variety of biological materials present good biocompatibility, including collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, based on the evaluation criteria.

Objective To compare the efficacy of sevoflurane combined anesthesia and propofol combined anesthesia in elderly patients undergoing abdominal surgery.Methods Sixty patients aged 65-80 yr scheduled for elective abdominal surgery under general anesthesia were randomly divided into 2 groups (n = 30 each): sevoflurane group (group S) and propofol group (group P).Anesthesia was induced with TCI of sufentanil and propofol.The initial target effect-site concentration (Ce) of sufentanil was set at 0.25 ng/ml and initial target plasma concentration (Cp) of propofol set at 2.0 μg/ml,and then both concentrations were increased in 0.5 μg/ml increment when they reached the balance until loss of consciousness.Tracheal intubation was facilitated with rocuromitm 0.6 mg/kg and the patients were mechanically ventilated.Anesthesia was maintained with inhalation of sevoflurane (end-tidal concentration set at 1.0%-2.0%) in group S,TCI of prepofol (Cp set at 2-4 μg/ml) in groups P, and sufentanil by TCI ( Ce set at 0.15 ng/ml) and iv infusion of vecurenium 0.15 mg·g-1·h-1 in both groups. .BIS value was maintained at 40-60,and changes of MAP and HR were less than 30% of the baseline value in both groups.The use of vasoactive drugs during perioperative peried,time of awakening,extubation and consciousness were recorded and compared between the 2 groups.Results The usage rate of urapidil was significantly decreased,and the awakening time,extubation time and consciousness time shortened in group S compared with group P.Conclusion Sevoflurane-sufentanil combined anesthesia provides better hemodynamic stability and faster recovery than propofol-sufentanil combined anesthesia in elderly patients.