1.The Selection of Transdermal Release Condition and Percutaneous Enhancer of Oxaprozin Gel in Vitro
China Pharmacy 2001;12(6):331-333
OBJECTIVE: To observe the effect of drug loading, medium composition, transdermal enhancer, lauryl alcohol and azone on percutaneous permeation of oxaprozin gel in vitro.METHODS: Drug permeation test was carried out by using modified Franz-type double compartment diffusion cell and isolated mice skin in vitro as transdermal barrier.RESULTS: Under the condition of an effective area of 5.77cm2, it was found that the result was stable and the reproducibility was well with drug loading more than 1.2g, the receiver solution ethanol to normal saline=7∶ 3(v∶ v);The transdermal enhancing effect of lauryl alcohol was superior to that of azone, the effect of mixed transdermal enhancer 3% AZ+ 10% LA was the best.CONCLUSION: The selection of both the optimum release condition and the best penetration enhancers provided reference for oxaprozin transdermal delivery.The standardization of gel percutaneous test in vitro was discussed preliminarily.
2.The primary study of auto-IgG on glycoL+ cell blocking EPO-receptor in patients with immunorelated pancytopenia.
Yi-hao WANG ; Rong FU ; Hui LIU
Chinese Journal of Hematology 2011;32(11):794-795
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Autoantibodies
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biosynthesis
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Case-Control Studies
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Child
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Female
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Humans
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Immunoglobulin G
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biosynthesis
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Male
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Middle Aged
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Pancytopenia
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immunology
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pathology
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Receptors, Erythropoietin
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physiology
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Young Adult
3.Two cases of epiglottic neuroendocrine carcinoma.
Hui LIU ; Yi ZHAO ; Xiaoguang HE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(19):1531-1532
Neuroendocrine tumors of the epiglottis were extremely rare. Patients normally had no obvious symptoms and signs of early stage. Epiglottis lingual except (without) tubercle of epiglottis, and hyperplastic mass were observed by using laryngoscopy and also confirmed by biopsy and immunohistochemistry. Immunohis- tochemical studies showed that Syn, a broad spectrum endocrine marker, and CgA, a specific marker of endocrine tumor, were both positive. Which is consistent with the diagnosis of neuroendocrine carcinoma.
Carcinoma, Neuroendocrine
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pathology
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Epiglottis
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pathology
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Humans
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Immunohistochemistry
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Laryngeal Neoplasms
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pathology
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Laryngoscopy
5.Expression of MT-3 mRNA in human esophageal squamous cell carcinoma
yi, MIAO ; bao-qing, LI ; hui-ning, LIU ; hui, LIU ; zi-qiang, TIAN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(03):-
Objective To detect the expression of metallothionein-3 (MT-3)mRNA in human esophageal squamous cell carcinoma. Methods Five cell lines of human esophageal cancer,TE-1,TE-13,TTN,ECA-109 (cell lines of esophageal squamous cell carcinoma) and OE33 (cell lines of esophageal adenocarcinoma),were used in this study. RT-PCR was employed to detect the expression of MT-3 mRNA. Peripheral blood monouclear cells from normal subjects were served as controls. Results Sequencing of RT-PCR product certified the gene of MT-3 mRNA. It was revealed by gel electrophoresis that there was expression of MT-3 mRNA in each cell line. The relative expression of MT-3 mRNA was 0.230?0.023,0.516?0.020,0.140?0.009,0.176?0.015 and 0.085?0.011 in cell lines of TE-1,TE-13,TTN,ECA-109 and OE33,respectively,significantly lower than that in controls (0.762?0.026) (P
9.Comparison of the efficacies of laparoscopic sleeve gastrectomy with duodenojejunal bypass and laparoscopic Roux-en-Y gastric bypass in the treatment of patients with non-obese type 2 diabetes mellitus
Hui LIANG ; Wei GUAN ; Huan LIU ; Qing CAO ; Yi MIAO
Chinese Journal of Digestive Surgery 2013;12(12):909-913
Objective To investigate the efficacies of laparoscopic sleeve gastrectomy + duodenojejunal bypass (DJB) and laparoscopic gastric bypass in the treatment of patients with non-obese type 2 diabetes mellitus (T2DM).Methods The clinical data of 42 patients with type 2 diabetes mellitus and body mass index (BMI) < 30 kg/m2 received surgical treatment at the First Affiliated Hospital of Nanjing Medical University from January 2012 to June 2013 were retrospectively analyzed.Fifteen patients received laparoscopic sleeve gastrectomy + DJB (Sleeve + DJB group),and 27 received Roux-en-Y gastric bypass (RYGB group).The follow-up time for all the patients was more than 6 months.The decrease of BMI,complete remission of T2DM,decrease of fasting glycemia and glycosylated hemoglobin (HbAlc),postoperative nutritional condition and the incidence of complications of the 2 groups were compared.The measurement data were analyzed using the t test and the repeated measurement chi-square test.Results The operation time of the Sleeve + DJB group and the RYGB group were (137 ± 61)minutes and (89 ± 43) minutes,with significant difference between the 2 groups (t =6.158,P < 0.05).No mortality and hemorrhage,bowel obstruction and anastomotic stenosis were detected.One patient was complicated with bile leakage in the Sleeve + DJB group,and was cured by conservative treatment 5 days later.The levels of fasting glucose before operation and at postoperative month 1,3,6 were (8.9 ± 0.7) mmol/L,(5.8 ± 1.3) mmoL/L,(5.6 ±1.8) mmol/L and (5.7 ± 0.3) mmol/L in the Sleeve + DJB group,and (9.9 ± 1.2) mmol/L,(6.9 ± 0.8) mmol/L,(6.6 ± 2.2) mmol/L and (5.6 ± 0.8) mmol/L,with no significant difference between the 2 groups (F =1.670,2.932,0.444,0.158,P > 0.05).The levels of HbAle before operation and at postoperative months 1,3,6 were 7.4%± 1.4%,6.5% ±0.6%,5.7%±0.5%,5.9% ±0.6% in the Sleeve + DJB group,and 7.7%± 2.0%,6.8% ± 1.3%,5.7%±0.8%,5.6% ± 1.1% in the RYGB group,with no significant difference between the 2 groups (F =0.055,0.125,0.005,0.286,P > 0.05).The remission rates of T2DM of the Sleeve + DJB group and the RYGB group were 14/15 and 74.1% (20/27) at postoperative month 6,with no significant difference between the 2 groups (x2 =2.320,P > 0.05).The decrease rate of BMI of the Sleeve + DJB group and the RYGB group were 18.2%±9.5% and 21.2% ± 4.9%,with no significant difference between the 2 groups (t =0.982,P >0.05).The numbers of patients with postoperative anaemia,avitaminosis and diarrhea were 0,0,2 in the Sleeve + DJB group and 3,2,6 in the RYGB group,with no significant difference between the 2 groups (x2=1.795,1.167,0.908,P >0.05).The BMIs of all the patients were above 19 kg/m2.Conclusion The effects and incidence of postoperative complications of Sleeve + DJB for the treatment of T2DM are comparable to those of RYGB.Sleeve + DJB has less interference on the nutritional condition of patients compared with RYGB.
10.CT perfusion study of neck lymph nodes
Jin ZHONG ; Jun LIU ; Rui HUA ; Hui QIAO ; Yi GONG
Chinese Journal of Radiology 2011;45(1):46-49
Objective To study the CT perfusion features of various lymph nodes in the neck.Methods Dynamic perfusion CT scanning was performed in 83 neck lymph nodes proved by pathology,including tuberculosis lymph nodes, lymphoma and metastatic lymph nodes. The shapes, blood flow modes,and perfusion parameters of these lymph nodes were compared among 3 groups. Statistical analysis of L/T and CT perfusion parameters was performed by one-way ANOVA and LSD test. Results The values of MTT of tuberculosis lymph nodes, lymphoma and metastatic lymph nodes were (28. 13 ±5.08), (31.08 ±5.82),and ( 11.24 ±5.31 ) s,respectively. The MTT of metastatic lymph nodes was statistically lower than that of tuberculosis lymph nodes and lymphoma (P < 0. 05). Their frequencies of marginal blood flow were 5/9,4/19, and 39/55 (70. 9% ), respectively. The frequency of marginal blood flow in the tuberculosis lymph nodes and metastatic lymph nodes was statistically higher than that of lymphoma ( P < 0. 05 ). Their frequencies of central blood flow were 2/9, 11/19, and 9/55 (16.4%), respectively. The frequency of central blood flow in the lymphoma was statistically higher than that of tuberculosis lymph nodes and metastatic lymph nodes ( P < 0. 05 ). Their values of L/T were 1.82 ± 0. 32, 1. 80 ± 0. 39, and 1.84 ± 0. 36,(40. 98 ±6. 62) s,respectively. There were no significant differences in L/T, BF, BV and TTP among tuberculosis lymph nodes, lymphoma and metastatic lymph nodes( P > 0. 05 ). Conclusion CT perfusion,especially combination functional imaging with perfusion images may be helpful in judging the nature of neck lymph nodes.