Adolescent ; Adult ; Dimethylformamide ; adverse effects ; Female ; Humans ; Kidney ; drug effects ; physiopathology ; Lipid Metabolism ; drug effects ; Liver ; drug effects ; physiopathology ; Male ; Middle Aged ; Occupational Exposure ; adverse effects ; Young Adult

Objective To establish a method for determination the contents of six phendic acids in Mailuoning injection by HPLC at same time. Methods Separation was performed on a Kromasil-C18 (4.6 mm? 250 mm, 5 ?m) column with mobile phase consisting of 0.1% phosphoric acid-water and Acetonitrile with gradient elute at the flow rate of 1 mL/min. The UV detection wavelength was set at 327 nm and the column temperature was set at 25 ℃. The sample size was 20 ?L. Results The standard cures of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, 3,5-Dicaffeoylquinic acid, 3,4- Dicaffeoylquinic acid were linear within the contentration range of 0.010 90～0.544 8 ?g (r=0.999 9), 0.024 06～1.203 2 ?g (r =1), 0.011 06～0.552 8 ?g (r =1), 0.015 94～0.796 8 ?g (r =1), 0.009 104～0.455 2 ?g (r =1), 0.010 37～0.518 4 ?g (r=1) respectively. And the recovery rates of them were 101.06%, 100.22%, 101.54%, 99.42%, 100.21%, 101.65% respectively. Conclusion The method appeared to be simple, reliable, accurate and can be used to determine the contents of six phendic acids in Mailuoning injection.

Objective:To develop the Chinese version of the 22-item Impact of Event Scale-Revised (IES-R-C) and examine its reliability and validity. Methods:A sample of 439 female offenders experiencing at least one traumatic life event was tested. The authors examined the IES-R-C's retest reliability, internal consistency, discriminative validity and predictive value of the cut-off. Results:The test-retest reliability coefficient was 0.86;The Cronbach's coefficient of the IES-R-C was 0.96;The mean inter-item correlation coefficients ranged from0.42 to 0.60;The correlation coefficient of the three factors with the total scale scores ranged from0.84 to 0.91; The correlation coefficient among the three factors ranged from 0.75 to 0.89; Posttraumatic stress disorder (PTSD) and partial PTSD cases indicated significantly higher scores than non-PTSD cases. 34/35 in the IES-R-C total score seemed an appropriate cutoff indicative of high levels of sensitivity (0.86), specificity (0.86), and efficiency (0.86) in the broadly defined PTSD-positive groups (PTSD + partial PTSD). Conclusion: the of IES-R-C is a reliable and valid measure for assessing the posttraumatic stress symptoms in Chinese-speaking sample. The IES-R-C may be a useful self-rating diagnostic instrument particularly for survivors with PTSD symptoms as a clinical concern (PTSD + partial PTSD) by using a 34/35 cutoff in total score.

Objective To investigate the relationship between the expression of laminin and laminin-receptor in pulmonary carcinoma and cancer metastasis as well as prognosis.Methods Techniques of immune histochemistry were used.Results The higher the pathological grade of pulmonary carcinoma was, the higher the level of expression of laminin was. There were significant differences betweenⅠgrade group andⅡgrade group and Ⅲ grade group and Ⅳ grade group. The expression of laminin was also related to lymph node metastasis (P

Adult ; Aged ; Breast Neoplasms ; genetics ; metabolism ; pathology ; surgery ; Female ; Gene Amplification ; Genital Neoplasms, Male ; genetics ; metabolism ; pathology ; surgery ; Humans ; Male ; Middle Aged ; Paget Disease, Extramammary ; genetics ; metabolism ; pathology ; surgery ; Paget's Disease, Mammary ; genetics ; metabolism ; pathology ; surgery ; Penile Neoplasms ; genetics ; metabolism ; pathology ; surgery ; Receptor, ErbB-2 ; genetics ; metabolism ; Scrotum ; Vulvar Neoplasms ; genetics ; metabolism ; pathology ; surgery

Acute Disease ; Asthma ; drug therapy ; Bronchodilator Agents ; administration & dosage ; adverse effects ; therapeutic use ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infusions, Intravenous ; Male ; Theophylline ; administration & dosage ; adverse effects ; analogs & derivatives ; therapeutic use ; Treatment Outcome

Objective To investigate the effect of intracoronary application of tirofiban on coronary slow flow patients with acute myocardial infarction during primary percutaneous coronary intervention (PPC1).Method It was a retrospective analysis of 187 patients with acute myocardial infarction treated with PPCI in the emergency department of Beijing Anzhen Hospital enrolled in this study from January,2008 through January,2011.The patients divided into 2 groups in terms of intra-coronary administration of tirofiban (tirofiban group) and intra-coronary use of nitroglycerol (control group).Data were statistically analyzed by using SPSS 13.0 software.Categorical variables were analyzed using x2 test and continuous variables were compared by t test.Results Between two groups,there were no differences in preoperative systolic pressure (P =0.245),the rate of TIMI flow 3 (P =0.568) after PPCI and ST segment resolution (P =0.824),LVEF (P =0.275) and in-hospital mortality (P =0.502).Compared with tirofiban group,the systolic pressure was lower and the rate of using intra-aortic counter-pulsation was higher in control group.Although the incidence of slight bleeding in the control group was lower than that in the tirofiban group,no severe bleeding was observed in both groups.Conclusions The effect of intracoronary use of tirofiban was similar to that of nitroglycerol in terms of improving slow flow of coronary artery.It could safely and effectively reduce the incidence of the coronary slow flow in the patients after PPCI,but it produced a little impact on systolic pressure.It may be a better method of choice for AMI patient with low blood pressure.

Cell Cycle Checkpoints ; drug effects ; Cell Line, Tumor ; Gene Expression Regulation ; drug effects ; Humans ; NF-kappa B ; metabolism ; Scorpion Venoms ; pharmacology

Objective In order to promote service lever,hospitals started to use mobile phone text messages to construct short message platform. Methods Based on mobile phone text message services provided by China Mobile Inc. and China Unicom Inc,hospitals formulated platform service procedures including the service on demand system,booking a whole month and broadcasting; designed a variety of interactive processes of short message for business. Results Such platform can be used to apply reservation,notification for PACS,LIS reports,services for in-patient,information for managers and transaction among employees. Conclusion With such platform,hospitals will build up friendly relationship between doctors and patients,improve office automation system,and upgrade service lever. Along with this event,the future will open out bright prospects for us.

Objective To establish the amplified luminescent proximity homogeneous assay(AlphaLISA) for the detection of Sendai virus.Methods The antigen concentration,serum concentration and the donor beads/acceptor beads ratio used in the AlphaLISA method were optimumized by the phalanx experiments, then the antibodies of Sendai Virus in 40 rat sera were detected by the established AlphaLISA method and ELISA detection method.The results were compared and the differentia between the two methods was confirmed by IFA.Results The optimum concentration of SV bio-peptide in AlphaLISA assay was 250 nmol/L, the best proportion of donor beads/acceptor beads ratio was 1 ∶1, using the concentration of 20 μg/mL and the serum dilution was 1∶10000.7 of the 40 rat sera were detected SV positive by ELISA, the positive rate was 17.5%, 8 of the 40 rat sera were determined SV positive by AlphaLISA, and the positive rate was 20.0%, the AlphaLISA positive serum was confirmed by IFA.Conclusions We preliminary established the Amplified Luminescent Proximity Homogeneous Assay(AlphaLISA) for the detection of Sendai virus.The sensitivity of the method is comparable to classical ELISA method, but this method use less serum samples and without washing steps.The method has some advantages in degeneracy and accuracy.