Objective By comparing the organ system based medical integration teaching mode and traditional teaching mode to evaluate the effect of the integration of curriculum implemen-tation. Methods Through a questionnaire survey of 63 students who implement integrated curriculum and 183 students who are not implementing integrated curriculum as well as 76 teachers, we evaluate students' emotional, cognitive and motor skills, using SPSS statistical analysis, chi-square test. Results In the affective domain, most of the teachers thought the experimental class was superior to the com-mon one in learning interest, lifelong learning and independent learning, critical thinking, the contrast of the two classes were P=0.000, P=0.031 and P=0.001 respectively, all of significant difference. In the cognitive domain, the contrast of the two classes in memory, understanding and application were P=0.000, all of significant difference. The experimental class has high degree of recognition. In the psychomotor domain, more than 55.3%(42/76) of the teachers thought that the experimental class was superior to the common class. According to the students' questionnaire survey, the contrast of the two classes in clinical operation skills, Interpersonal communication skills were P=0.077, P=0.031 respec-tively. There was no statistically significant difference. Conclusions The integrated curriculum teach-ing model can greatly promote students' abilities in most areas such as interest in learning, lifelong learning awareness, clinical thinking ability; interpersonal communication skills etc. In some respects the differences remains to be further studied such as memory of knowledge, systematic knowledge, and clinical operation skills, etc.

Objective Light acts as an important zeitgeber and regulates the body's circadian rhythm.Excessive lighting at night leads to light pollution,which interferes with the circadian rhythm and thus adversely affects the body.To observe the adverse effect of continuous light on female reproductive endocrinology and explore effective treatment,the animal experiment was carried out.Methods 185 sexual maturity female SD rats in the age of 2 months with normal estrous cycle were enrolled.60 rats were selected randomly and enrolled into control group.Normal illumination conditions were given.The remaining rats were given 300±20lux light intensity for 24h/d last for 50 days to get reproductive rhythm disorder model.The model rats (100) were randomly divided into models group (n =50) and Traditional Chinese Medicine (TCM) group (n =50).Traditional Chinese Medicine group was treated with sequential TCM.At last we get 50 rats enrolled into model group and herbal group respectively.The TCM group rats were given therapy of nourishing yin and tonifying yang sequential TCM,while the two other groups were gavaged the same volume of physiological saline.The estrous cycle was observed in all groups.20 days later,blood were collected dynamically and E2 (estradiol),P (progesterone),LH (Luteinizing Hormone),FSH (Follicle-Stimulating Hormone)and melatonin were compared to evaluate the therapeutic effect of TCM.Finally,try to explain the mechanism of TCM's effect from the perspective of melatonin.Results At the first day,E2 was lower in TCM group and models group than control group (P＜ 0.05).At the second day,E2 was higher in traditional Chinese Medicine group than control group (9＜0.05).At the third day,E2 was higher in TCM group and models group than that in control group (P＜ 0.05).Progesterone in control group showed low level in pre-estrous and estrous period and elevated in the late stage of estrous.Progesterone showed no obvious change during the estrous cycle in TCM group and models group.At the third and fourth day,Progesterone was significantly lower in models group than TCM group and control group.At the first day,LH in models group [(249.8±50.2)mIU/mL] and TCM group [(83.4±37.3)mIU/mL] were significantly lower than control group [(430.0± 100.4)mIU/mL] (P＜0.05).At the first day,FSH in models group and TCM group were significantly lower than control group (P＜0.05).At the fourth day,FSH in TCM group was significantly lower than control group and models group (P＜0.05).Compared the melatonin among three groups,the circadian rhythm was high at night but low in the day in control group.The highest level was nearly 5 times higher than the lowest level.Neither the models group nor the TCM group had obvious recent rhythm changes.Conclusion Continuous light can induce disordered estrous cycle and loss of rhythm.Traditional Chinese Medicine can somehow induce circulation reconstruction.Continuous light can induce disordered endocrine,E2,FSH,LH surge abnormal,ovulation failure,luteal phase defect.TCM can induce ovulation and luteal support.Continuous light can destroy melatonin circadian rhythm and TCM can increase melatonin,which could be the mechanism of TCM's effect.

Objective: Cell cycle has recently become more appealing as a new target of anti-carcinogen-ic agent. Diallyl disulfide (DADS) inhibits growth and induces call cycle G_2/M arrest in human gastric cancer BGC823 cells. Cell division cycle protein 25C (Cdc25C) and CyclinB1 expression are involved in G_2/M arrest.However, mechanisms of G_2/M arrest are not yet fully understood. The aim of this study was to elucidate the mechanism of cell cycle G_2/M arrest in human gastric cancer BGC823 cells induced by DADS. Methods: The expression of chk1 and Chk2 mRNA associated with cell cycle arrest of BGC823 cells after the induction with DADS for 1 or 2 days was detected by RT-PCR. The protein expression of cycle-related proteins ATM-RAD3-related gene (ATR), checkpoint kinase1 (Chk1), checkpoint kinase 2 (Chk2), P-ATR, P-Chk1 and P-Chk2 was measured by Western blot. Interaction between Chk1/2 and Cdc25C was analyzed by immuno-precipitation. Results: After the cells were treated with 15 mg/L DADS for 1 or 2 days, the expression of Chk1 and Chk2 mRNA was not significantly different from that in untreated cells (P>0.05). Western blot analysis showed that the expression of total Chk1 and Chk2 treated with 15 mg/L DADS was not significantly different from that in untreated cells. But phospho-chk1 showed a significant increase after stimulation with 15 mg/L DADS for 2h to 12h and continued to increase gradually as time went on (P<0.05). Phospho-Chk2 showed a eak expression and a weaker expression after stimulation with DADS, but the changes were not statistically significant (P>0.05). Addition of 15 mg/L DADS to BGC823 cells for 15 rain to 120 min resulted in an increase in phospho-ATR expression, whereas no changes were found in ATR expression (P<0.05). The Chk1 Ab in-creasingly precipitated Cdc25C in BGC823 cells treated with DADS (P<0.05). In contrast, Chk2 Ab failed to change precipitation with Cdc25C by DADS (P>0.05). Conclusion: Activation of chk1 was involved in cell cy-cle G_2/M arrest in BGC823 cells treated with DADS. Cell cycle G_2/M arrest by DADS is associated with phos-phorylation of several cell cycle regulatory proteins including ATR and Chk1 which regulate expression of Cdc25C.

Objective To explore the applicability of pubertal development scale in estimating sexual maturation among urban Chinese adolescents.Methods A cross-sectional survey was conducted among 2562 students of 4th to 12th grade in Shanghai,selected by stratified cluster sampling method.Self-reported pubertal maturation level was obtained by Pubertal Development Scale (PDS).Meanwhile,visual depiction of Tanner stages of breast,genital and pubic hair development were rated by a trained rater of the same gender,Results In 2562 students,the correlation between self-reported PDS and assessment from the raters were substantial,both in boys and girls (Pearson' s correlation coefficients:0.822 and 0.814,respectively).The overall agreement of pubertal development stages based on PDS and the rater' s assessment were 35.24％ and 51.42％ for boys and girls,respectively,with Kendall' s correlation coefficients as 0.713 and 0.685,respectively.Adolescents who were older,had relatively higher level of pubertal stages or adolescents from the richer families,tended to underestimate their actual level of pubertal maturation while those male subjects and overweight or obese subjects were even more likely to be so.Conclusion Using the self-reported PDS,we could conveniently obtain the necessary information regarding sexual maturation among the urban adolescents.It seemed especially useful in the related epidemiological survey when physical examination was precluded by cost,privacy and other concerns.

Objective To identify the epidemiological features of COVID-19 epidemic in Chenzhou City, Hunan province so as to provide scientific evidence for effective containment of the COVID-19 epidemic. Methods Descriptive epidemiological method was used to retrospectively characterize the confirmed COVID-19 cases and asymptomatic cases in Chenzhou City from January 23 through March 10, 2020. Results A total of 39 confirmed COVID-19 cases and 6 asymptomatic cases infection were documented in the city, with no death.We identified 8 clusters of COVID-19, which were all familial transmission.There was statistical difference between the sources of different types of epidemic (χ²=15.996, P < 0.001), in which all the local COVID-19 cases were the secondary cases in the clusters.As the epidemic expanded, the trend shifted from imported-case-centered to local-case-centered.The epidemic has covered 81.81% of the city area; in each area, the first COVID-19 cases were all imported. Conclusion The COVID-19 epidemic has been effectively controlled.At present, we focus on the quarantine in the entry and exit to prevent the imported epidemic.

OBJECTIVETo establish a HPLC-based method for simultaneous determination of 2 classes of compounds (flavonoids and chromones) and 6 their effective components,(including prin-O-glucosylcimifugin, cimifugin, 4'-O-beta-D-glucosyl- 5-O-methylvisamminol, quercetin, sec-o-glucosylhamaudol and formononetin), in Yupingfeng Decoction.

METHODSHPLC-based separation of the agents was performed on Agilent Extend-C(18) column (4.6 mm x 250 mm, 5 microm) at 25 degrees with the mobile phase of MeOH-1% acetic acid water solution (gradient elution), flow rate of 0.8 ml/min and detection wavelength of 254 nm.

RESULTS AND CONCLUSIONHPLC allowed simultaneous quantitative determination of the 6 components in Yupingfeng Decoction, and they showed good linear relationships when their sample amount ranged 90-1810 ng, 97-1940 ng, 190-1906 ng, 105-3144 ng, 88-2625 ng and 109-3279 ng, respectively, with correlation coefficients all beyond 0.9999 and average recovery rates of 98.2%, 99.1%, 97.3%, 97.8%, 98.8% and 99.2%, respectively. This simple and convenient method accommodated a broad linear range with high sensitivity and precise and reproducible results.

Chromatography, High Pressure Liquid ; methods ; Chromones ; analysis ; Drugs, Chinese Herbal ; chemistry ; Flavonoids ; analysis ; Isoflavones ; analysis ; Quercetin ; analysis ; Reproducibility of Results

Objective To establish an isolated rat pancreas perfusion technique,a method for the precise measurement of insulin secretion in vitro.Methods An isolated rat pancreas perfusion technique was applied in the study of insulin secretion from?-cells in 10 high-fat diet-induced obese Wistar rats.Results For the assessment of the functional integrity of the perfused pancreas,the isolated pancreas of 6 rats met all the criteria: (1)The constancy of perfusion pressure was kept over the whole experiment time[(70?5)mm Hg,1 mm Hg= 0.133 kPa].(2)The duodenal peristaltic activity of isolated pancreas and duodenum block was present after perfusion experiment.(3)Total insulin response to arginine stimulation was significantly increased as compared with glucose stimulation[maximum insulin secretion rate:(987?100)?U/min vs(545?50)?U/min,P

The use of free flaps is an essential and reliable method of reconstruction in complex head and neck defects. Flap failure remains the most feared complication, the most common cause being pedicle thrombosis. Among other measures, thrombolysis is useful when manual thrombectomy has failed to restore flap perfusion, in the setting of late or established thrombosis, or in arterial thrombosis with distal clot propagation. We report a case of pedicle arterial thrombosis with distal clot propagation which occurred during reconstruction of a maxillectomy defect, and was successfully treated with thrombolysis using recombinant tissue plasminogen activator. We also review the literature regarding the use of thrombolysis in free flap surgery, and propose an algorithm for the salvage of free flaps in head and neck reconstruction.

The use of free flaps is an essential and reliable method of reconstruction in complex head and neck defects. Flap failure remains the most feared complication, the most common cause being pedicle thrombosis. Among other measures, thrombolysis is useful when manual thrombectomy has failed to restore flap perfusion, in the setting of late or established thrombosis, or in arterial thrombosis with distal clot propagation. We report a case of pedicle arterial thrombosis with distal clot propagation which occurred during reconstruction of a maxillectomy defect, and was successfully treated with thrombolysis using recombinant tissue plasminogen activator. We also review the literature regarding the use of thrombolysis in free flap surgery, and propose an algorithm for the salvage of free flaps in head and neck reconstruction.