Objective To prepare Mtb8.4 gene vaccine and to study the cellular immune response induced by the vaccine. Methods The gene encoding Mtb8.4 from Mycobacterium tuberculosis was amplified by PCR and cloned into the eukaryotic expression vector pcDNA3.1 (+). C57BL/6N mice were vaccinated three times with Mtb8.4 gene vaccine at 3 weeks interval. Four weeks after the final inoculation, mice were sacrificed to assess cytokine response and CTL induction. Results The IFN-? and IL-2 titers were 787.317?45.586pg/ml and 319.953?57.978pg/ml in Mtb8.4 gene vaccine group, 1 486.540?39.600pg/ml and 767.043?50.269pg/ml in BCG group, respectively. The level of IL-4 in BCG group (90.580?10.998 pg/ml) increased significantly as compared to other groups (P

Objective To observe the specific cellular immune response induced by co-immunization of DNA vaccine of Mtb8.4 and plasmid encoding human interleukin 12(hIL-12)in mice.Methods Fifteen C57BL/6N mice were divided into following groups:Mtb8.4 gene vaccine plus plasmid of hIL-12,Mtb8.4 gene vaccine,BCG,empty vector alone and PBS.Mice were immunized intramuscularly in both hind limbs three times at the intervals of three weeks or once subcutaneously with 1?106 of viable M.bovis BCG Pasteur at the time of the first DNA immunization.The level of IFN-? in supernatant of spleno-lymphocyte cultures was measured by ELISA.CTL activities of spleno-lymphocyte were detected with LDH release assay.Results The levels of IFN-? and IL-2 in supernatant of spleno-lymphocyte cultures in the group of Mtb8.4 gene vaccine plus plasmid of hIL-12 were significantly higher than that of group of Mtb8.4 alone(P

Objective To study the role of the chimeric hsp70/CD80 DNA vaccine for treatment for TB. Methods C57BL/6N mice challenged with H37Rv were immunized with the chimeric hsp70/CD80 DNA vaccine, hsp70 DNA vaccine and BCG in order to compare the therapeutic role of these vaccines. Results The levels of interferon ? (IFN-?) in the serum of mice in hsp70/CD80 group (1336.98?129.64) pg/ml was significantly higher than in group BCG (121.54?56.39) pg/ml, pcDNA3 (192.00?64.36) pg/ml and pcDNA3 hsp70 (542.33?99.77) pg/ml. The growth of Mycobacterium tuberculosis in live and spleen were inhibited significantly in hsp70/CD80 vaccinated mice. Conclusions The chimeric hsp70/CD80 DNA vaccine was more efficient than BCG and hsp70 DNA vaccine alone in treating TB.

BACKGROUND: Bone cement coated by different materials has various characteristics and causes varying therapeutic effects. OBJECTIVE: By comparing characteristics of CPC, CPC/D, and CPC/M/D3 to investigate the preparation of doxorubicin microspheres-coated bone cement. METHODS: Doxorubicin microspheres were prepared with multiple emulsion solvent volatilixation method. Doxorubicin microspheres were mixed with CPC as the ratio of 3:7 to prepare doxorubicin microspheres-coated bone cement. The samples were randomly divided into three groups: CPC group, containing bone cement alone; CPC/D group, containing doxorubicin;CPC/M/D group, containing doxorubicin microspheres. Scanning electron microscope at varying magnification was used to observe structural characteristics and measure the diameter of microspheres. X-ray diffraction was used to estimate the extent of CPC and CPC/M/D samples. The initial and final setting time of cement samples in the three different groups was measured at 25 ℃ and 37℃ respectively. The injectability and interval porosity of different samples were tested. The compressive strength of the specimens was measured using a universal material testing machine to record the maximal compressive strength and breaking strength. RESULTS AND CONCLUSION: PLGA microspheres (100-150 μm) were globular and the surface was slick and sly. Micrestructure of bone cement was not obviously changed following mixing with drugs, thus the location and characteristics of drugs in bone cement were not determined. Micrespheres-coated bone cement (100-150 μm) was distributed among CPC powder. All the X-ray diffraction pattern of three different samples was in coincidence with standard X-ray diffraction pattern of hydroxyapatite, i.e., the major peak was located near 32°. Additional drugs and microspheres did not cause new phases. Obvious collapsing was not observed in the three samples following immediately adding in saline, but the collapsing appeared in both CPC/D and CPC/M/D samples after 24 hours. The setting-up time of CPC/M/D was the longest, but that of CPC was the shortest. On the other hand, the setting-up time was the longest at 37℃. The final setting-up time of CPC/M/D group was 45 minutes. The doxorubicin microspheres-coated bone cement showed the best property of injectability among the three kinds of cement. The interval porosity was the highest in the CPC/M/D group but the lowest in the CPC group. Interval porosity of doxorubicin microspheres-coated bone cement was up to 61.67%. The yield stress was the strongest in the CPC group but the weakest in the CPC/M/D group. Additionally, the yield stress of calcium phosphate cement dramatically decreased while doxrorubicin microspheres were coated. However, there was no significant difference between them. The preparation of doxorubicin microspheres-coated bone cement was reliable and the product had good structures and properties.

Objective To compare the efficiencies of imatinib and dasatinib in patients with chronic myeloid leukemia-chronic phase (CML-CP).Methods The databases were retrieved,including Cochrane Library,OVID,Embase,PubMed,China National Knowledge Infrastructure (CNKI),WanFang database and VIP database,besides,references of articles were further to search.The quality of randomized controlled trials (RCTs) was assessed by the Cochrane collaboration' s risk tool.Meta-analysis was performed by RevMan 5.1 software.Results A total of 5 articles involved 2 031 patients with CML-CP were included.Meta-analysis showed that the rate of complete cytogenetic response (CCyR) at the 12th month in dasatinib group was higher than that in imatinib group [83.6 ％ (478/572) vs 70.6 ％ (406/575),OR =2.11,95 ％ CI 1.59-2.80,P＜ 0.05],and the rate of major molecular response (MMR) at the 12th month in dasatinib group was higher than that in imatinib group [49.3 ％ (296/600) vs 30.6 ％ (185/605),OR =2.22,95 ％ CI 1.75-2.82,P ＜ 0.05].Conclusion Dasatinib can improve CCyR and MMR rate at the 12th month in CML-CP patients.

This study left flavonoids and alkaloids Chinese herbal monomer with common parent nucleus as cream base carriages drug respectively, cream base were prepared with stable span 60-tween 80 emulsification system. The near-infrared stability analysis technology was performed to quantitatively characterize the physical stability of cream. Base on the theory of gel network structure, theory of emulsification, theory of solubility parameter and theory of double layer, the influence mechanism of Chinese herbal monomer on physical stability of cream was discussed. The results showed that tetrahydropalmatine, matrine and naringenin had similar solubility parameter value with cream base material, creams prepared with those Chinese herbal monomer have higher Zeta potential value and stronger physical stability, and that those creams had similar microstructure information with cream base. However, a larger solubility parameter difference exists between baicalin, baicalein, berberine, palmatine and cream base material. Creams prepared with those Chinese herbal monomers had lower Zeta potential value and poorer physical stability, and that those creams had great different microstructure information with cream base.
Drug Stability ; Drugs, Chinese Herbal ; chemistry ; Emulsions ; chemistry ; Kinetics ; Skin Cream ; chemistry ; Solubility

Objective To investigate the differences in the epidemiological characteristics of patients in pre-hospital medical care before and after “5 · 12” Wenchuan earthhquake in Deyang city.Methods Comparison was made between the epidemiological features of cases treated in Deyang emergency medical center from the January 1,2007 through the December 31,2007 before the earthquake (the pre-earthquake group) and those treated from the January 1,2009 through the December 31,2009 after the earthquake ( the post-earthquake group) with the criteria of international classification of diseases (ICD-10).Results ( 1 ) There were 11 325 patients medically cared before earthquake and 16 265 patients after disaster.The distribution of sex,age,the top five causes,the seasonal and diurnal variations of emergency call,radius of service circle of pre-hospital medical care cases were no significantly different between the two groups ( P ＞ 0.05 ).( 2 ) The time required for scheduling ambulance and time consumed for the transportation of patients in the post-earthquake group were less than those in the pre-earthquake group ( P ＜ 0.05 ).Conclusions The efficiency of medical care done by 120 Emergency Command Center in Deyang city has been improved significantly in ease of without change in epidemiological features of patients after reconstruction of disaster area.

BACKGROUND: Angiogenesis attracts much attention in tissue engineering field. Previous research has proved that a two-dimensional culture of vascular endothelial growth factor (VEGF) promotes angiogenesis.OBJECTIVE: To evaluate the effect of VEGF on three-dimensional angiogenesis.METHODS: Endothelial progenitor cells were separated from the SD rat bone marrow. At about 70%-80% fusion, rat tail collagen gel was added to establish three-dimensional models. Samples in the experimental group were incubated in complete culture solution containing M199 culture media, fetal bovine serum, VEGF, and double antibody. The samples in the control group were incubated with VEGF-free culture media. In vitro culture and amplification of bone marrow-derived endothelial progenitor cells were determined at 1, 4, 7, and-20 days after incubation. Morphology and quantitative analysis were performed at 3, 6, 9, and 12 days after three-dimensional model establishment.RESULTS AND CONCLUSION: Endothelial progenitor cells grew from three-dimensional matrix into collagen matrix in the experimental group. Budding and infiltration were observed in the collagen within 24 hours, and branching-like structure was then gradually formed. Cells in the control group grew slowly, with slowing budding, small tubiform structure, superficial infiltration into COllagen, sparse network structure, and non-intact. Numbers of newborn vessels in the expedmental group were significantly greater than control group (P<0.01). A detection on gel block showed positive expressions of endothelin-1 and endothelial nitric oxide synthase-3 on the 3~(rd), 6~(th), 9~(th), and 12~(th) days. The results demonstrated that VEGF mobilized and induced endothelial progenitor cells in order to promote angiogenesis. Rat tail collagen gel induced endothelial progenitor cells which behaved migration, proliferation, and pullulation of angiogenesis.

OBJECTIVETo evaluate the relationship between overweight, obesity and arterial stiffness in community residents.

METHODSA total of 4585 community-dwelling adults in Jiangsu province, China were surveyed with the method of stratified and cluster sampling from 2007 to 2009. Overweight and obesity were defined by body mass index (BMI) and arterial stiffness was assessed by brachial-ankle pulse wave velocity (baPWV). Statistical analysis of arteriosclerosis included multivariate logistic regression testing among which BMI was viewed as continuous variable (1 kg/m(2) increasing to BMI) and categorical variables (underweight, normal, overweight and obesity) respectively. Odds ratio, population attributable risk percent and the optimal cut-off points for BMI to evaluate arteriosclerosis were analyzed using receiver operator characteristic (ROC) curve.

RESULTS(1) After age control, BMI of male or female were positively correlated with baPWV (r = 0.213, P < 0.01; r = 0.186, P < 0.01). baPWV and prevalence of arteriosclerosis were significantly higher in obese residents compared with normal body weight group (all P < 0.01). (2) As a continuous variable, the odds ratio value of BMI on predicting arteriosclerosis was 1.146 (95%CI: 1.117 - 1.175, P < 0.01) after adjusting of age, gender and hypertension. As categorical variables, the odds ratio value of BMI was 0.369 (95%CI: 0.141 - 0.962, P < 0.05) for underweight group, 1.576 (95%CI: 1.333 - 1.863) for overweight group and 2.087 (95%CI: 1.615 - 2.698) for obesity group (all P < 0.01). (3) The population attributable arteriosclerosis risk was 19.1% and 11.6% in overweight and obesity groups, respectively. The area under the ROC curve was 0.661 (95%CI: 0.645 - 0.678, P < 0.01) and the optimal cut-off point for BMI to evaluate arteriosclerosis was 24.25 kg/m(2).

CONCLUSIONSOverweight and obese residents faced higher risk for arteriosclerosis than normal population. Overweight and obesity are independent risk factors for arteriosclerosis after adjusting for age, gender and hypertension.

Adult ; Aged ; Ankle Brachial Index ; Arteriosclerosis ; Blood Flow Velocity ; Body Mass Index ; China ; Female ; Humans ; Hypertension ; Male ; Obesity ; complications ; physiopathology ; Overweight ; complications ; physiopathology ; Prevalence ; Pulsatile Flow ; Pulse Wave Analysis ; Thinness ; Vascular Stiffness

Objective To evaluate the therapeutic effects and safety of open surgery vs.endovascular procedures for subclavian steal syndrome (SSS).Methods A retrospective analysis was made on 112 patients from July 2005 to July 2014 including 72 undergoing endovascular therapy and 40 cases treated by open bypass surgery.Results There were not difference between endovascular therapy group and surgical group in technical success rate (95.8％ vs.100％,P =0.239),postoperative subclavian artery diameter [(8.3 ± 1.6) mm vs.(8.1 ± 0.7) mm,P =0.374],postoperative suffering/contralateral systolic pressure ratio [(0.95 ±0.12) vs.(0.96 ±0.15),P =0.518].Nor there were the differences in mortality [0(0％) vs.3 (7.5％),P =0.018],postoperative renal dysfunction [2 (2.8％) vs.5 (12.5％),P =0.042],wound inffections [1 (1.4％) vs.4 (10％),P=0.034],duration of operation [(1.5 ±0.32) h vs.(2.7±0.51) h,P=0.027],days in hospital [(5.7±3) dvs.(9.3±3) d,P=0.013].After 1,3,5 years postoperative follow-up the two groups were not significantly different in patency rates,subclavian artery diameter,suffering/contralateral systolic pressure ratio (all P ＞ 0.05).Conclusion For subclavian artery steal syndrome,endovascular treatment is safe,effective and minimally invasive.