Objective To investigate the influencing factors on the nursing ability of caregivers who provide service for elderly Methods patients with cardiovascular disease in medical and nursing combination institutions. A total of 276 caregivers who provide service for elderly patients with cardiovascular disease in medical and nursing combination institutions in Beijing urban area were selected as research subjects using the judgment sampling method. The Chinese Caregiver Task Inventory, eHealth Results Literacy (eHL) Scale and Sense of Coherence (SOC) Scale were used to investigate the nursing ability, eHL and SOC. The scores of nursing ability, eHL and SOC of the caregivers were (28.3±6.1), (17.0±5.9) and (69.0±9.4), respectively. The results of multiple linear regression analysis showed that caregivers who regarded the occupation as a career, had general or good subjective income, cared for disabled subjects, had a good basic knowledge of cardiovascular disease and qualified eHL had P P Conclusion relatively high nursing ability (all <0.01). The higher the SOC score, the higher the nursing ability ( <0.01). Occupational recognition, subjective income, master the basic knowledge of cardiovascular diseases, disability of nursing patients, eHL and SOC were factors affecting the nursing ability of caregivers who provide service for elderly patients with cardiovascular diseases in urban medical and nursing combination institutions.

Objective To explore the method of health education to guide medication for the senile patients. Methods Fifty senile patients were divided into two groups;control group(n =25) and study group (n=25). The control group was given routine medication instruction while the study group performed medication instruction by using image pictures. Results The patients in the study group had dramatically improved the cognitive level of medicine. Conclusions The medication instruction of using image pictures is superior to the routine medication instruction.

Objective To study the related factors of hyponatremia in the elderly patients with chronic heart failure in order to provide evidences for preventing and nursing of hyponatremia.Methods Clinical data of 143 patients over 65 years old were investigated.The patients were divided into hyponatremia group (n =47)and control group (n =96).There were 29 cases with more than 80 years older and 19 cases with 65-79 years older in hyponatremia group.The data from the two groups were compared.Results The patients in hyponatremia group were significantly older than these in control group [(88.5 ± 4.8) vs (82.5 ± 7.5),t =4.895,P ＜0.01),and the incidences of hypertension,pneumonia,diabetes and acute heart failure,as well as the usage of diuretics and nasogastric tubes in hyponatremia group were significantly higher than that in the control group(x2 =26.034,14.492,5.385,10.881,12.674,5.389;P ＜ 0.05).Very elderly patients needed longer time to achieve normal natrium level in blood serum than that elderly patients (t =5.069,P ＜ 0.01).Conclusions Hyponatremia of the elderly patients with chronic heart failure was related to hypertension,pneumonia,diabetes,acute heart failure,usage of diuretics and nasogastric tubes.The symptoms of hyponatremia in very elderly patients is atypical,which are very likely to be neglected.We should pay more attention to the special population.

Objective To discuss the psychology and behavior of elderly hypoglycemia patients with symptomatic carotid artery stenosis, and compare the differences in psychology and behavior between groups.Methods Measurement and assessment with fall efficacy scale on psychology towards prevention of fall among 408 elderly hypoglycemia patients (＞ 60 years old) with symptomatic carotid artery stenosis were taken; They were divided for hypoglycemia history, carotid artery stenosis and ischemia sympton, and fall history; SPSS 17.0software was applied to do statistical analysis. Results There is significant difference on fall efficacy scale score between groups with and without hypoglycemia, carotid artery stenosis and ischemia sympton, and fall history (P ＜0. 0 1). Conclusions Subdividing symptomatic groups, applying preventive intervention procedure on prevention of fall, education on psychology and behavior towards prevention of fall are the effective psychological intervention measures on prevention of fall.

Objective To explore the clinical pathway of operable and evaluable psychological nursing for elderly patients so as to improve the psychological nursing competence of duty nurses.Methods Sixty elderly patients were randomly divided into control group( n =30 cases) which received traditional psychological nursing and pathway group( n =30 cases) which received psychological nursing in clinical pathway.Then,patients' satisfaction to psychological nursing service and the effect of psychological nursing pathway on competence of duty nurses were compared.Results The rate of emotional attention in nurses,psychological nursing content,mastery of relax skill,mental support feeling and patients' satisfaction in pathway group was 96.67％,86.67％,93.33％,100％,respectively,which were higher than 63.33％,36.67％,6％,36.67％,80％in control group,and difference was statistically significant (x2 =10.42,15.86,45.06,27.80,4.04,respectively;P ＜0.05 or P ＜0.01 ).The score of health education,assessment and intervention,communication and cooperation of nurses after clinical pathway application of psychological nursing respectively was (94.00 ±0.42),(87.61 ±7.09),(98.40 ±0.69) significantly higher than (92.80 ±0.49),(61.3.0 ±9.50),(93.70 ±1.24) before application (t =11.76,14.04,20.95,respectively; P ＜ 0.05 ).Conclusions Application of psychological nursing pathway is good for improving competence of nurses and promoting psychological nursing service in geriatric internal medicine wards.

OBJECTIVESTo study the effect of lead acetate on the expression of nerve growth factor (NGF) protein in rat brain and the regulation of thyroid hormone.

METHODSLead acetate was given to SD rats intraperitoneally ip. at the dosage of 25, 50 and 100 mg/kg respectively. 6-n-propyl-2-thiouracil (PTU) was used to make a hypothyroid model and then lead acetate was given at the dosage of 50 mg/kg body weight through i.p. The NGF protein expression in rat brain was observed by immunohistochemistry Triiodothyronine (T3), thyroxin (T4), TSH in serum and T3, T4 in brain tissue were determined by radio immunoassays (RIAs).

RESULTSThe average gray value of NGF protein in cerebral cortex of 50 mg, 100 mg treated groups (180.49 +/- 10.33, 169.72 +/- 19.75, respectively) were lower than the control (200.75 +/- 3.27, P<0.01). The area density of NGF protein in hippocampus of three treated groups (0.08 +/- 0.14, 0.12 +/- 0.02, 0.13 +/- 0.04, respectively) were significantly different from the control (0.025 +/- 0.015, P<0.05). The area density and the average gray value of NGF protein in lead acetate treated hypothyroid rat brain were of no significant changes. The levels of serum T3 in three treated groups [(0.68 +/- 0.02), (0.57 +/- 0.04), (0.54 +/- 0.02) microg/L respectively] and T4 [(28.30 +/- 1.83), (27.35 +/- 2.55), (24.00 +/- 3.01) microg/L] in serum were significantly lower while TSH [(6.34 +/- 1.13), (7.74 +/- 0.79), (9.16 +/- 0.77) IU] higher than those in the control [T3 (0.97 +/- 0.14) microg/L, T4 (54.50 +/- 3.70) microg/L and TSH (4.62 +/- 2.16) IU], and there was a good dose-response relationship. The levels of T3 in cerebral cortex of three treated groups [(13.26 +/- 0.81), (11.49 +/- 0.10), (10.42 +/- 1.19) pg/mg pro respectively] and T4 [(0.50 +/- 0.03), (0.49 +/- 0.13), (0.42 +/- 0.01) ng/mg pro] were significantly lower than those in control [(20.85 +/- 11.01) pg/mg pro, (0.76 +/- 0.14) ng/mg pro, P<0.05, P<0.01].

CONCLUSIONLead could increase the NGF protein expression in rat brain, which may be regulated by thyroid hormone.

Animals ; Brain Chemistry ; drug effects ; Immunohistochemistry ; Male ; Nerve Growth Factor ; analysis ; Organometallic Compounds ; toxicity ; Rats ; Rats, Sprague-Dawley ; Thyroid Hormones ; analysis ; blood ; physiology

OBJECTTo investigate the therapeutic effect of microencapsulated porcine retinal pigmented epithelial cells (RPE-M) transplantation on rat model of Parkinson's disease (PD).

METHODSPrimary porcine RPE cells were harvested by enzyme digestion and expanded in culture medium. Determine the levels of dopamine (DA) and homovanillic acid (HVA) by high performance liquid chromatography electrochemical (HPLC) assay, and the levels of brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF) were detected by ELISA. Alginate-polylysine-alginate (APA) microencapsulated cells were produced by using a high voltage electrostatic system. PD rat model was established by unilateral injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle (MFB). After that, the RPE-M was transplanted into the corpus striatum of PD rat, and then the rotation test scores were recorded and biochemical changes of the corpus striatum were tested.

RESULTSThe levels of DA, HVA, BDNF and GDNF secreted by RPE were stable in the RPE culture supernatant and were not changed by the microencapsulation. Eighty-three percent rats developed PD by unilateral lesion of 6-OHDA in the MFB. The RPE-M transplantation had therapeutic effect on 33% PD rats.

CONCLUSIONPorcine RPE cells grow actively in vitro and could secrete DA, HVA, BDNF, and GDNF constantly, which does not be affected by the passage culture and the APA miroencapsulation. RPE-M transplantation of may be a curative therapy for PD.

Adrenergic Agents ; toxicity ; Animals ; Brain-Derived Neurotrophic Factor ; metabolism ; Cell Transplantation ; methods ; Cells, Cultured ; Disease Models, Animal ; Dopamine ; metabolism ; Enzyme-Linked Immunosorbent Assay ; Epithelial Cells ; metabolism ; transplantation ; Glial Cell Line-Derived Neurotrophic Factor ; metabolism ; Male ; Oxidopamine ; toxicity ; Parkinson Disease ; etiology ; surgery ; Rats ; Rats, Sprague-Dawley ; Retina ; cytology ; Swine ; anatomy & histology ; Time Factors ; Transplantation, Heterologous ; methods ; Tyrosine 3-Monooxygenase ; metabolism

BACKGROUND: It is quite difficult to produce a decellularized lung scaffold, in which cells are removed and the extracellular matrix components (ECM) are preserved effectively. Perfusion of detergent-enzymes is an effective method with wide applications for decellularized lung scaffolds. OBJECTIVE: To investigate the effects of two detergents (sodium deoxycholate, SDC and sodium dodecyl sulfate, SDS) on the preparation of decellularized lung scaffolds. METHODS: Twenty-four male Sprague-Dawley rats were randomized into three groups: control group with no intervention, SDC group and SDS group. Decellularized lung scaffolds were prepared by perfusion of SDC or SDS combined with enzymes. The rat lung tissues in the three groups were taken for histological staining, immunofluorescent staining and DNA quantification. A549 cells were cultured and seeded onto the decellularized lung scaffolds for 7 days followed by hematoxylin-eosin staining. The decellularized lung scaffolds prepared by perfusion of SDC or SDS were subcutaneously implanted into the rat back, and the implants were retrieved and assessed by Masson staining after 2 weeks. RESULTS AND CONCLUSION: In the control group, there were abundant cells in the lung tissues. In the other two groups, the decellularized lung scaffolds were nearly transparent, and the morphology of the SDC scaffold was more close to the native lung. There were no residual cells and nuclei on the two scaffolds, and the DNA content in the SDS and SDC groups was significantly lower than that in the control group (P＜ 0.01). At 7 days of culture, A549 cells cultured on the SDS and SDC scaffolds migrated from the edge to the center of the scaffold. Comparatively speaking, the migration ability of A549 cells on the SDC scaffolds was stronger, and there was obvious cell invasion and growth in the middle part of the lung. After 2 weeks of scaffold transplantation, the SDC implants poorly fused with the surrounding tissues, with a clear boundary, a large number of infiltrating cells distributed evenly, and intravascular blood cells were clearly visible; the number of new blood vessels with larger diameter in the SDC scaffold was significantly higher than that in the SDS scaffold. These findings indicate that the SDC scaffold has better biocompatibility than the SDS scaffold, which can fuse with the surrounding tissues faster and produce more infiltrating cells and new blood vessels.

Objective To investigate the prognostic value of acute beart block (AHB) after percutaneous transluminal septal myocardial ablation (PTSMA) in patients with hypertrophic obstructive cardiomyopathy (HOCM). Methods Ninety-four HOCM patients underwent PTSMA were included in this study. Twelve-lead electrocardiograms were obtained during and post PTSMA. Association between clinic events and incidence of post-PTSMA AHB was analyzed. Results AHB was induced in 26 patients by PTSMA and disappeared in 11 patients shortly post PTSMA, subacute intraventricular conduction disturbances was seen in 11 (42.3%), subacute I°AVB in 2(7.7% ) and subacute Ⅲ°AVB in another 2 (7.7%) patients. Among 68 patients without AHB during PTSMA, intraventricular conduction disturbances was evidenced in 14 patients (20.6% ), I°AVB in 2 (2.9% ) and Ⅲ°AVB in 1 patient (1.5%) after PTSMA. AHB patients with subacute heart block were associated with poor prognosis (conduction block duration was 42.00 h) while patients without AHB was associated with benign prognosis even with new onset of subacute heart block (conduction block duration was 7.33 h, P <0.01). Conclusion Patients with AHB during PTSMA are at higher risk for subacute heart block, especially intraventricolar conduction disturbances. AHB patients with subacute heart block were associated with poor prognosis and longer recovery time conducting system.

Objective To investigate the on-job training mode suitable for ICU nursing staff for elderly patients,in order to improve the comprehensive treatment and nursing capabihties of ICU nursing staffs in critically ill elder patients with a variety of diseases.Methods Nurses from coronary care unit (CCU),respiratory intensive care unit (RICU),and surgery intensive care unit(SICU) were recruited to participated in the training.Every three months were considered as a training cycle,each ICU took responsibility for one-month training program for the participants in turns.Satisfaction rate and examination scores concerning the first aid theory and first aid skills of nurses were evaluate and compared before and after training.Results After receiving the training program,satisfaction rates for the first aid theory and the first aid skills of nurses in patients and their families were 96.6％ and 95.2％,and higher than that were 83.7％ and 86.3％ before the training program; 95.5％ and 93.7％ among doctors after the training were higher than 80.9％ and 84.6％ before the training program; 98.7％ and 97.6％ among nursing staffs after the training were higher than 80.9％ and 84.6％ before the training program; and these differences were statistically significant between before and after the training (x2 =8.900,4.395,5.224,4.940,12.890,7.680,respectively; P ＜0.05).The examination score of the first aid theory before the training was (59.2 ± 4.2) and lower than that was (89.7 ± 3.7) after the training,the examination score of the first aid skills of nurses before the training was (63.8 ± 4.6) and lower than that was (90.3 ± 5.3) after the training,these differences were statistically significant between before and after the training (t =-59.12,-41.06,respectively; P ＜ 0.01).Conclusions The establishment of the teaching mode of multidisciplinary collaboration on nursing training among elderly patients in ICU,which can develop the comprehensive treatment and nursing capabilities of ICU nursing staffs and improve the satisfaction rate for nursing job and the rate of successfully rescuing critically ill patients,can be used to achieve the sharing of human resources,medical instruments and information resources,and is feasible in nursing practice in ICU and more worthy of clinical promotion and application.