1.A case report of inborn pyloric duplication.
Li-Qun ZHOU ; Bing-Hui WANG ; Ya-Hua ZUO
Chinese Journal of Contemporary Pediatrics 2007;9(5):421-421
Child
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Female
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Gastroscopy
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Humans
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Pylorus
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abnormalities
2.Cutaneous melioidosis: report of a case.
Qun LI ; Hui-ming ZENG ; He-jun ZHANG
Chinese Journal of Pathology 2006;35(12):767-768
Anti-Infective Agents
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therapeutic use
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Burkholderia pseudomallei
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drug effects
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isolation & purification
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Humans
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Male
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Melioidosis
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drug therapy
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microbiology
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pathology
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Middle Aged
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Skin Diseases, Bacterial
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drug therapy
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microbiology
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pathology
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Trimethoprim, Sulfamethoxazole Drug Combination
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therapeutic use
3.Effect of adiponectin on the expression of osteoprotegerin and receptor activator of nuclear factor-kappa B ligand in osteoblasts
Qun LI ; Bi HUANG ; Hui XIE ; Lingqing YUAN
Chinese Journal of Tissue Engineering Research 2009;13(28):5485-5488
BACKGROUND: The main role that the osteoprotegedn (OPG) plays in bone tissues is to inhibilate the formation and the activity of osteoclast, while as for the receptor activator of nuclear factor-κB ligand (RANKL), it is to stimulate the differentiation and the activity of osteoclast. Both OPG and RANKL are important for the regulation of osteoclastic function. Recent studies have found the stimulative effects of adiponectin on the proliferation and the differentiation of osteoblast, as well as the coupling between adiponectin and bone metabolism. But effects of adiponectin on osteoclast remain unclear. OBJECTIVE: To investigate the effect of adiponectin on the expression of OPG and RANKL in osteoblast, and to further analyze its effects on the formation of osteoclast. DESIGN, TIME AND SETTING: A contrast observational experiment was conducted in the laboratory of Institute of 2008.MATERIALS: Cancellous bone in anterior superior lilac spine was obtained from adult normal by surgery for cell incubation. Clinical samples were provided by the Xiangya Hospital. METHODS: Human osteoblast was incubated with different doses of adiponcetin (0, 3, 10 and 30 mg/L) for 48 hours, after which OPG and RANKL mRNA and protein expression level was determined. In addition, adiponcetin was added into the co-culture system of osteoblast and peripheral blood monouclear cells for examing its effects on osteoclast formation. MAIN OUTCOME MEASURES: OPG and RANKL mRNA and protein expression in human osteoblast was determined using fluorescent quantitative polymerase chain reaction (PCR) and enzyme linked immunosorbent assay (ELISA). Osteoclast was detected by antitartaric acid acid phosphatase staining. RESULTS: Adiponcetin inhibited osteoblast OPG mRNA and protein expression in a dose-dependent way (P < 0.05). Adiponcetin promoted osteoblast RANKL mRNA and protein expression in a dose-dependent way (P < 0.05). Adiponectin induced the osteoclasts formation in the co-culture system of osteoblast and peripheral blood monouclear cells. CONCLUSION: Adiponectin has the effect of inducing osteoclast formation via stimulating RANKL expression and inhibiting OPG
4.Microbial Ecology Principle of Activated Sludge Acclimation
Tie-Qun ZHU ; Kai-Hui LI ; Jie ZHANG ;
Microbiology 1992;0(06):-
Activated sludge is a complicated microbial ecosystem that has diversity. Based on the diversity,microorganisms are selected by acclimation condition:the survival of the fittest,otherwise eliminate through selection or contest. Moreover,microorganisms in activated sludge acclimatize themselves to acclimation conditions. In the process the ecosystem distributes and adjusts microbial niche over again. The theory of "selection and acclimatization" may be used to explain the mechanism of activated sludge acclimation. So then,original sludge,wastewater quality and treating process are major influencing factors for activated sludge acclimation.
5.Level of reduced glutathione and oxidized glutathione in a mouse bone cell line MC3T3-E1 cells exposed to fluoride
Zhi-tao, ZHAO ; Li-qun, SHI ; Peng, L(U) ; Hui, XU ; Guang-Sheng, LI
Chinese Journal of Endemiology 2012;31(5):511-514
Objective To observe the level of reduced glutathione(GSH) and oxidized glutathione(GSSG)in a mouse bone cell line MC3T3-E1 cells exposed to fluoride.Methods MTT method was used to detect cell viability of M C3T3-E1 cells exposed to varying concentrations and periods of fluoride [F-concentration:0(control),0.5,1.0,2.0,4.0,8.0,12.0,20.0 mg/L; F-periods:1,2,4 and 10 days].The Xevo TQ MS was employed to test the levels of GSH,GSSG and glutamine (Gln).Results The MC3T3-E1 cell viability was significantly higher in the 2 mg/L group(0.57 ± 0.05) 1 day after the exposure compared to the respective control(0.49 ± 0.03,P <0.01); conversely,cell viability was markedly lower in the 8 mg/L(0.49 ± 0.07) and 12 mg/L(0.47 ± 0.09)groups 4 days after the exposure in comparison to the control(0.63 ± 0.06,P < 0.05 or P < 0.01).The cell viability in the 8 mg/L group(1.52 ± 0.29) 10 days after the exposure was significantly higher than that in the control group (0.86 ± 0.23,P < 0.01),however,the value in the 20.0 mg/L group (0.54 ± 0.07) was significantly lower(P <0.01).The level of cell GSH decreased significantly in the 20 mg/L groups 2 days[(13.92 ± 4.63)μmol/L]and 10 days [(0.53 ± 0.30)μmol/L]after exposure compared to the respective comtrols [(26.42 ± 3.67),(24.85 ± 5.68)μmol/L,all P < 0.01].The level of cell GSSG markedly increased in the 2 mg/L group 2 days [(1.12 ± 0.62)μ mol/L]and the 8 mg/L group 4 days [(2.13 ± 0.62)μ mol/L]after exposure compared to the controls[(0.55 ± 0.22),(1.46 ± 0.46)μmol/L,all P < 0.05].The similar change was observed in the 8 mg/L group[(2.97 ± 1.30)μmol/L] 10 days after exposure compared to the control [(1.35 ± 0.50)μmol/L,P < 0.05].The level of Glndecreased significantly in the 2 mg/L group[ (62.80 ± 17.4l)μ mol/L] 4 days and in the 8 and 20 mg/L groups 10 days[ (122.26 ± 19.51), (19.38 ± 8.11)μmol/L] after exposure compared to the controls [ (83.28 ±14.32), ( 147.15± 16.95) μmol/L , all P < 0.05 or P < 0.01 ]. Conclusions Fluoride exposure can significantly promote the changes of GSH, GSSG and Gln levels in the osteoblast, thus affecting the intracellular redox equilibrium.
7.Morinda Officinalis How improves cellphone radiation-induced abnormality of LH and LHR in male rats.
Rong LI ; Wei-qun YANG ; Hui-qin CHEN ; Yong-hong ZHANG
National Journal of Andrology 2015;21(9):824-827
OBJECTIVETo investigate the effects of Morina Officinalis How (MOH) on the abnormal levels of serum luteotrophic hormone (LH) and LH receptor (LHR) in the testis tissue induced by cellphone radiation (CPR) in rats.
METHODSFifty adult male SD rats were randomly divided into five groups of equal number: sham CPR, untreated CPR, negative double distilled water (DDW) control, aqueous MOH extract, and alcohol MOH extract. All the animals were exposed to mobile phone radiation except those of the sham CPR group. Then, the rats of the latter two groups were treated intragastrically with MOH at 20 g per kg of the body weight per day in water and alcohol, respectively. After 2. weeks of treatment, all the rats were sacrificed for measurement of the levels of serum LH and LHR in the testis tissue.
RESULTSThe levels of serum LH and LHR were 30.15 ± 8.71 and 33.28 ± 6.61 in the aqueous MOH group and 0.96 ± 0.06 and 0.94 ± 0.08 in the alcohol MOH group, both significantly decreased as compared with the negative DDW controls (P < 0.05), but with no remarkable difference between the two MOH groups (P > 0.05).
CONCLUSIONMOH can improve CPR-induced abnormality of LH and LHR in adult male rats.
Animals ; Cell Phone ; Electromagnetic Radiation ; Luteinizing Hormone ; blood ; drug effects ; radiation effects ; Male ; Morinda ; chemistry ; Radiation Injuries, Experimental ; blood ; drug therapy ; Random Allocation ; Rats ; Receptors, LH ; blood ; drug effects ; radiation effects ; Testis ; radiation effects
9.Meningeal melanocytoma with nevus fuscoceruleus ophthalmomaxillaris: report of a case.
Chun WU ; Hai WANG ; Qun-li SHI ; Heng-hui MA ; Zhen-feng LU
Chinese Journal of Pathology 2011;40(3):194-195
Adult
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Diagnosis, Differential
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Humans
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MART-1 Antigen
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metabolism
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Magnetic Resonance Imaging
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Male
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Medulloblastoma
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metabolism
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pathology
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Melanocytes
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pathology
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Melanoma
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diagnosis
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metabolism
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pathology
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surgery
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Melanoma-Specific Antigens
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metabolism
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Meningeal Neoplasms
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diagnosis
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metabolism
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pathology
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surgery
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Neoplasms, Multiple Primary
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diagnosis
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metabolism
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pathology
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surgery
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Neurilemmoma
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metabolism
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pathology
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Nevus of Ota
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diagnosis
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metabolism
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pathology
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surgery
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S100 Proteins
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metabolism
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Skin Neoplasms
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diagnosis
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
10.Secretory carcinoma of breast in male: report of a case.
Yan XU ; Qun-Li SHI ; Xiao-Jun ZHOU ; Heng-Hui MA ; Hang-Bo ZHOU
Chinese Journal of Pathology 2009;38(10):707-708
Adenocarcinoma, Mucinous
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metabolism
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pathology
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Adult
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Breast Neoplasms, Male
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metabolism
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pathology
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surgery
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Cadherins
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metabolism
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Carcinoma
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metabolism
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pathology
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surgery
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Carcinoma, Signet Ring Cell
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metabolism
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pathology
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Diagnosis, Differential
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Follow-Up Studies
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Humans
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Keratin-5
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metabolism
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Lymph Node Excision
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Male
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Mastectomy
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methods
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Mucin-1
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metabolism
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S100 Proteins
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metabolism