Organ transplant the patient be special mental state of regulate, the budget consume a control, organ resources a development exploitation and transplant a performance evaluation is current check and supervision human body organ transplant the work open an exhibition of key problem. Need the angle which learns from the ethics, the knowledge which uses science strengthens to cure the communication for suffer from and strengthen the confidence that the sufferer cure and use to publicly line up with the realistic case of the sufferer's equilibrium mindset of the method maintenance resolve mental state to regulate a problem; The thought which learns with the ethics examines closely sufferer's psychological stress and learn from the ethics of the angle be excellent to turn a profect to lower expenses and use publicly in procession sufferer's equilibrium mindset of the method maintenance; Paww the social foundation of extensive organ source of the publicity extension and insistobtaining maintenance ethics to learn a principle and insistin a medical science by lqw the standard insures the science exploitation of organ; Pay attention to the transplantation performance of the method evaluation of use the economicsand pay attention to pay attention to resolve the transplantation performance of the corpse organ to maximize a problem towards live body organ the risk performance of the transplantation evaluate with, help the clinical organ to transplant a smoothly open of the work an exhibition.

Colitis, Ulcerative ; classification ; diagnosis ; pathology ; Humans ; Inflammation ; Medicine, Chinese Traditional

Objective To establish the method of determining the content of lead in Cynomorium Songaricum Rupr.by atomic fluorescen spectrometry.Method After digested by perchloric acid-nitric acid,atomic fluorescen spectrometry was used to determining the content of lead in Cynomorium Songaricum Rupr..Results When the concentration of trace lead was 0.00～10.00 ng/mL,it had linear relationship with fluorescence intensity.The linear function was Y =408.459 3X-72.159 5,and the linear correlation coefficient was 0.999 5.The relative standard deviation of six determination results of the samples was 2.8%.The recovery rate was 93.7%～96.0%.Conclusion The method is featured by easy operation,fast speed and good sensitivity.

Objective To investigate the clinical distribution characteristics of persisters isolated from the chronic infected pa-tients,so as to provide scientific basis for effective clinical measures to prevent,control and treat persister-associated chronic infection.Methods Clinical microbial samples cultured from Jan.2013 to Dec.2014 were analysed by WHONET5.6.Four bacteria species with the highest isolation rate were performed for screening of chronic infection.Concentration of bacteria were detected by viable plate count method and then the growth curve were drew of each sample under the presence of anti-biotics.Persisters were comfirmed according to the specific growth curve under the presence of antibiotics.Results Four highest isolated bacteria species of the clinical samples were Escherichia coli (1 3.2%),Klebsiella pneumoniae (9.6%), Pseudomonasaeruginosa (8%)and Staphylococcusaureus (6.6%).862 chronic infection samples were generated out of 14 216 microbial samples and 41 persisters (4 strains of Escherichia coli,23 strains of Klebsiella pneumoniae,8 strains of Pseudomonasaeruginosa and 6 strains of Staphylococcusaureus)were isolated finally.Conclusion With such a comprehen-sively retrospective analysis of the hospital clinical microbial samples,can tell that the ratio of persisters in chronic infection was not high.And chronic infections are mostly caused by gene-mutated drug-resistant bacteria.However,the isolation rate of Klebsiella pneumoniae was relatively high,of which more attention should be payed to the prevention and control.

Objective:To study the influence of anesthesia with propofol or with ketamine on autonomic nerve function in children. Methods: 35 children aged 3-8 years old undergoing general anesthesia for palatoplasty with ASAⅠ-Ⅱ cleft palate were randomly divided into 2 groups, group Ⅰ(ketamine group,n=17)and group Ⅱ (propofol group, n=18).The low frequency (LF), high frequency (HF), ratio of low frequency and high frequency (LF/HF),total power (LF+HF), MAP, HR and SpO_2 were recorded before anesthesia (T0), after intubation (T1), incision of skin(T2), 1 h after the begining of operation(T3), after the completion of operation(T4) and extubation (T5). Results:In the 2 groups the values of LF, HF, LF/HF and LF+HF at T1-T4 were significantly lower than those at T0 (P0.05). In group Ⅱ,the indexes of HRV at T2-T5 were significantly lower than those in group Ⅰ(P

Many kinds of small heat shock proteins (sHSPs) are able to prevent protein aggregation in stress, which show the ATP independent chaperone-like activity. The smallest protein HSP12.1 in sHSP family of the nematode Caenorhabditis elegans exhibits chaperone-like activities in vitro. It prevents protein aggregation in a certain extent when use insulin, ADH and lysozyme as the substrates, though it is not as efficient as the typical chaperones (such as HSP16.1 in C. elegans). By contrast, the other three sHSP12s (HSP12.2, HSP12.3 and HSP12.6), which have similar molecular masses and primary structure, appear devoid of in vitro chaperone-like activities. In addition, overexpressing HSP12.1 enhances cell thermotolerance of Escherichia coli. The survival rate of the HSP12.1 overexpressed cells is 4-fold higher than the control, yet whether it does the same function in C. elegans is still unknown. Results indicate that C-terminal region is not necessary for the chaperone-like activity of sHSPs, for HSP12.1 terminates a short C-terminal tail. N-terminal domain may play a relatively important role in the exhibition of chaperone-like activities, while ?-crystalline domain may also involve in this function.

DQA1*0501.TSHR peptides of 183～198、195～210、248～263、301～320、343～362 and 357～376 could bind with high affinity to both HLA-DR3 and HLA-DQA1*0501,all of their IC50 values less than(1 ?mol/L),but they could bind to neither HLA-DR7 nor HLA-DQA1*0201 with high affinity.Conclusion Epitopes of TSHR 183～198,195～210,248～263,301～320,343～362 and 357～376 on the TSHR extracellular domain might be the auto-antigens to cause GD.

Antigens, CD34 ; metabolism ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Hemangioma ; metabolism ; pathology ; surgery ; Hemangiosarcoma ; pathology ; Humans ; Median Nerve ; pathology ; Median Neuropathy ; metabolism ; pathology ; surgery ; Melanoma ; pathology ; Middle Aged ; Peripheral Nervous System Neoplasms ; metabolism ; pathology ; surgery ; S100 Proteins ; metabolism

Objective To study the prognostic value of modified early warning score (MEWS) and Acute Physiology and Chronic Health Evaluation (APACHE Ⅱ) score in emergency ICU elderly patients.Methods 113 elderly patients were collected in emergency ICU,after admission to calculate the MEWS score and APACHE Ⅱ score,the MEWS score and APACHE Ⅱ score difference were compared between death group and survival group.the differences were compared in MEWS score ≤ 3,4 ～6,7 ～ 9,≥ 10 scores groups respectively;analysis of the MEWS score and APACHE Ⅱ the relevance score.the correlation analysis of the MEWS score and APACHE Ⅱ score was given.Results The MEWS(7.91 ± 2.42) point and APACHE Ⅱ (21.9 ± 4.18) point in death group were greater than that in survival group [(4.51 ± 2.14) point,(19.53 ± 4.37) point] (t =7.49,2.70,all P ＜ 0.01).With MEWS score increasing,the fatality rate rose(P ＜0.01).the MEWS score and APACHE Ⅱ score was correlated(r =0.617,P ＜ 0.01).Conclusion The MEWS score was a simple and quick scoring system,as well as APACHE Ⅱ,it can predict the prognosis of elderly patients in emergency department,it has important application value.

Objective To assess the value of combination of susceptibility weighted imaging (SWI)and dynamic susceptibility-weighted contrast-enhanced (DSC) perfusion-weighted magnetic resonance imaging in astrocytoma grading.Methods SWI and DSC scans were performed in 82 patients with pathologically confirmed astrocytoma.The patient group consisted of grade Ⅱ (15),grade Ⅲ (10),and grade Ⅳ (57).The intratumoral susceptibility signal intensity (ITSS) and relative cerebral blood volume (rCBV) max were used to determine the grade of astrocytomas by Kruskal Wallis test,Welch test,Spearman correlation coefficients,Pearson correlation coefficients,and receiver operating characteristic curve (ROC)statistic methods.Results There were no ITSS in 14 cases of low-grade astrocytomas,the degree of ITSS were grade 1 to 3 in anaplastic astrocytomas,the degree of ITSS were grade 3 in all of the glioblastomas,the degree of ITSS were significant difference in all grades (H =71.96,P ＜ 0.01).rCBV max in grade Ⅱ,grade Ⅲ and grade Ⅳ astrocytomas were 1.26 ± 0.42,3.59 ± 2.09 and 8.34 ± 1.16 respectively,rCBV max were significant difference in all grades (F' =681.72,P ＜ 0.01).ITSS showed significant correlation with rCBV max (r =0.72,P ＜0.01) and tumor grades (r =0.89,P ＜0.01),and rCBV and tumor grades showed significant correlation (r =0.78,P ＜ 0.01).Area under the ROC curve application SWI,DSC,SWI and DSC in differentiation of the grade Ⅱ and grade Ⅲ astrocytomas were 0.99,0.93,1.00,differentiate grade Ⅲ from grade Ⅳ were 0.70,0.94,0.94,and differentiate high-grade from low-grade astrocytomas were 1.00,0.99,1.00.Conclusions ITSS is helpful to determine the grade of astrocytomas.The use of SWI in combination with DSC may improve the diagnostic accuracy of astrocytoma grading.