3.Study on the relationship between early lactate clearance and APACHEⅡ in critically ill patients
Qinglong LU ; Ping ZHAO ; Zengxiang MA ; Wensheng WANG ; Hui YANG
Chinese Journal of Primary Medicine and Pharmacy 2011;18(3):307-308
Objective To observe the relationship between early lactate clearance and APACHEⅡ in critically ill patients. Methods In 121 critically ill patients,APACHE Ⅱ and lactate clearance rate were recorded. According to APACHE Ⅱ score,all patients were divided into≤10 score group, (11 ~20) score group,(21 ~30) score group and > 30 score group, then compared the level of the early lactate clearance rate. The early lactate clearance rate were also compared between survival group and death group. Then the relationship between early lactate clearance and APACHE Ⅱ were analyzed. Results In( 11 ~20) score group,the early lactate clearance rate was lower than those in ≤ 10 score group, but the difference was not significant ( P > 0. 05 ). The early lactate clearance rate in (21 ~ 30) score group ( 18. 35 ± 10. 01 ) % was lower than those in ( 11 ~ 20) score group (27.35 ± 10. 22) % ( t = 3.481, P < 0. 01 ),in > 30 score group( 11.98 ± 9. 93 )% those was lower compared with (21 ~30) score group( t = 2. 968, P < 0. 01 ). In death group, APACHE Ⅱ score(28. 1 ± 6. 7 ) was higher than that in survival group ( 18. 8 ± 8. 4) ( t = 3. 030, P <0. 01 ), the early lactate clearance rate was lower ( t = 3. 619, P < 0. 01 ). APACHE Ⅱ score correlated well with the mean level of the early lactate clearance rate ( r = - 0. 641, P < 0. 01 ). Conclusion The lactate clearance rate was the good fator on evaluation of condition and prognosis in the critically ill patients.
4.Molecular signaling pathways in embryonic development of the parathyroid gland and the hypoparathyroidism
Ping LING ; Hui LU ; He JIANG ; Xue CAO ; Weiying ZOU
Chinese Journal of Endocrinology and Metabolism 2015;31(10):917-920
Hoxa3-Pax1/Pax9-Eya1-Six1/4 regulatory pathway seems to be operating during forming the bilateral parathyroid/thymus common primordial in early embryonic development.The specification of the parathyroid domain in the parathyroid/thymus primordial is regulated through a Shh-Tbx1-Gcm2 pathway.Gcm2 also may play roles in later steps of parathyroid development,including CaSR and PTH gene expression.MafB and Gcm2 interact with each other and synergistically activate PTH transcription.Genetic basis and the etiology of some hypoparathyroid disorders in man are involved defects in transcription factors that include GCMB,GATA3,Tbxl,SOX3 and GNA11.This marker expression in thymus and parathyroid primordium includes HoxA3,Pax1,Eya1,and Six1;and expression of parathyroid cell-like cells includes Gcm2,CaSR,and PTH.These expressions may serve as markers of stem cell differentiation into parathyroid cell-like cells.
5.The study of chronic angio-complications in diabetes mellitus and impared glucosetolerance patidents were diagnosticated newly
Lian-Ping WANG ; Na LU ; Zhao-Hui ZHU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(12):-
Objective To investigate the chronic angio-complications of diabetes mellitus(DM)and impared glucosetolerance(IGT)patients were diagnosticated newly.Methods 46 patients with diabetes mellitus and 97 pa- tients of impared glucosetolerance were chosen in the study,and 35 patients were chosen as normal glucose tlerance group.Some biochemical criterion,carotid artery intima-media thickness and plaque condition were measure.Results The group of IGT contract coronary heart disease is 62.9 %,cetebrovascular disease is 20.6%,carotid artery atheromatosis is 50.5 %,the group of DM contract coronary heart disease is 65.2%,cerbrovascular disease is 26,1%,carotid artery atheromatosis is 69.6%,it have statistics significance compared with control group(P
6.The Development of Symptom Interference and Symptom Clusters of HCC
Hui LI ; Qian LU ; Ping YANG ; Wanrong LIU ; Yanhua DONG
Chinese Journal of Practical Nursing 2015;31(14):1090-1092
The hepatocellular carcinoma (HCC) is one of the most common malignant tumors.Patients with hepatobiliary cancer suffer from multiple symptoms,such as physical symptoms and psychology symptoms.In recent years,along with the further research of symptoms,the concept of symptom clusters occurred.Those two issues have always been the hot topics in recent years.Through the efforts of experts at home and abroad,there has been a part of progress in this area.However,the results are not the same.There are lots of disputes in the numbers、sequence、incidences of the symptom clusters.The paper will discuss this issue from symptom experience and symptom clusters in order to provide the reference and basis for symptom management.
7.Reversal of drug resistance in human ovarian cancer cells by wild-type PTEN gene and its mechanisms
Hui-Juan WU ; Hai-Tao WU ; Dan-Hui WENG ; Hui XING ; Yun-Ping LU ; Ding MA ;
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
Objective To examine expression of PTEN gene in ovarian cancer cisplatin-sensitive cell line OV2008 cells and cisplatin-resistant cell line C13K cells,and evaluate the effect of wild-type PTEN gene on reversing cisplatin-resistance of C13K cells and underlying mechanisms.Methods The expression of PTEN mRNA and protein in OV2008 and C13K cells were detected by semi-quantitative RT-PCR and western blot.Recombinant eukaryotic expression plasmid containing human wild-type PTEN gene was transfected into C13K cells by lipofectamine 2000.The expression of PTEN mRNA was monitored by RT- PCR and the expression of PTEN,protein kinase B(AKT),phospho-AKT(p-AKT)protein were analyzed by western blot in PTEN transfected and untransfected C13K cells.Proliferation and chemosensitivity of cells to cisplatin were measured by methyl thiazolyl tetrazolium(MTT),and cell apoptosis was detected by flow cytometry after treatment with cisplatin.Results(1)The expression of PTEN mRNA and protein(1.02 ?0.05,1.02?0.07)in OV2008 cells were significantly higher than those in C13K cells,which were 0.45 ?0.03 and 0.55?0.03 respectively(P
8.Clinical application of serum GFAP,NDKA and PARK7 in patients with ischemic stroke
Guiping CUI ; Ping LIU ; Ping YANG ; Hui LU ; Jianwei LIANG ; Wei ZHANG
Chinese Journal of Laboratory Medicine 2011;34(9):779-784
Objective To explore the relationship of GFAP, NDKA and PARK7 serum concentrations of patients with IS, and their diagnose and prognosis value in IS. MethodsThe serum concentrations of GFAP, NDKA and PARK7 were detected in 37 IS patients, 28 ICH patients and and 30 healthy persons by ELISA. These indexes of patients were detected in 12 hours, 3 d and 14th day after onset of ischemic stroke. Their neurological injury status were also evaluated by MESSS at corresponding time points, and their activities of daily living were evaluated by BI at 14 d discharge from hospitaL At the same time, the diagnostic efficiency was analysed for IS using the three biomarkers and the combined detection. ResultsIn IS group, the serum concentrations of GFAP in 12 hours, 3rd and 14th day after onset were (5. 49 ±2. 25 )μg,/L, (5. 17 ± 2. 29) μg/L and (5. 96 ± 2.39 ) μg/L, respectively. The serum concentrations of NDKA were 9. 15(6.28 -12.79) μg/L, 9. 13(6.31 - 12.23) μg/L, 9.31(6.40 - 11.83) μg/L respectively,and the serum concentrations of PARK7 were (32. 71 ±6. 34 ) μg/L, (31.23 ±6. 04) μg/L, (32. 79 ±6. 94) μg/L respectively. The serum levels of GFAP, NDKA and PARK were respectively (4. 62 ± 1. 56)μg/L, 4. 24(3. 30 -5. 61 ) μg/L, ( 14. 25 +2. 65) μg/L in healthy control group. The levels in IS groups were remarkably increased compared with the healthy control group except the level of GFAP in the 3rd day (t = 1. 129, P>0. 05). The levels in other time points were significantly different between patients group and healthy control. t value of GFAP were respectively 2. 642, 1. 870,P<0. 05; Z value of NDKA were 6. 173, 6.100, 6.278,P <0. 01; t value of PARK7 were 14.964, 15.367,16.060, P <0. 01. The specificity and sensitivity of the individual detection for diagnosis of IS was 46. 7% (14/30) and 81.1%( 30/37 ) for GFAP, 90. 0% ( 27/30 ) and 78.4% ( 29/37 ) for NDKA, 96. 7% (29/30) and 97.3% ( 36/37 )for PARK7. The specificity and sensitivity for combined detection of 3 biomarkers was 96. 7% (29/30) and 100% (37/37). The combined detection achieved better specificity and sensitivity. Moreover, the risk of IS with higher level GFAP was 1. 3 times that of the controls ( OR = 1. 300, P = 0. 044 ). The risk of higher NDKA was 1.7 times higher( OR = 1. 668, P = 0. 036 ). The risk of higher PARK7 was 1.8 times higher (OR = 1. 809, P =0. 005 ). The serum levels of GFAP were significantly different between IS and ICH in 12 h(t= 4.097, P=0.000). The serum concentrations of GFAP, NDKA and PARK7 were positively correlated with MESSS score at different time points. In IS, r value were 0. 534, 0. 482, 0. 357 , P < 0. 05at less than 12 h; r value were 0.433, 0.487, 0. 299,P value were 0. 007, 0. 002, 0.073 at 3 d;r value were 0. 394, 0. 200, 0. 084,P value were 0.016, 0. 236, 0.620 at 14 d. And the serum levels of GFAP,NDKA and PARK7 were negatively correlated with BI score at 14th day, r value were -0. 430, -0. 321,-0.076,P value were 0.044,0.050,0.657. Conclusions The concentrations of GFAP, NDKA and PARK7 in serum are closely related with IS. The increased seruro levels of these indexes are risk factors in IS. The detection of these indexes could be helpful for the early diagnosis, timely treatment and prognosis assessment for IS.
9.Expression of subtilisin-like protease gene from Hirsutella sinensis before and after infecting Hepialus gonggaensis.
Zeng-Hui LU ; Ping SHI ; Shi-Jiang CHEN
Acta Pharmaceutica Sinica 2013;48(7):1164-1168
To address the role of Pr1 gene in the process of Hirsutella sinensis infecting Hepialus gonggaensis, differential expression of subtilisin-like protease gene was detected. In the present study, Pr1 gene analogues from H. sinensis were obtained by PCR strategy using specific primers designed from conserved regions of Pr1 gene reported in the GenBank. Then we detected the changes in the expression of Pr1 gene before and after infecting H. gonggaensis using real-time quantitative PCR. We obtained the partial sequence of Pr1 gene with the length of 535 bp (GenBank accession: KC009680). Real-time PCR results showed that the expression level of Pr1 gene was significantly different among 8 samples (P < 0.01). Pr1 gene showed the obvious higher expression level (2-3 folds) after infecting the H. gonggaensis, suggesting that the Pr1 gene may play an important role in the process of H. sinensis infecting H. gonggaensis. The present study paves a way for further identification on infectivity assessment of H. sinensis.
Amino Acid Sequence
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Animals
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Hypocreales
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genetics
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metabolism
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pathogenicity
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Larva
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Lepidoptera
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microbiology
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Real-Time Polymerase Chain Reaction
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Subtilisin
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genetics
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metabolism
10.Chinese Sanqi industry status and development countermeasures.
Xiu-Ming CUI ; Lu-Qi HUANG ; Lan-Ping GUO ; Da-Hui LIU
China Journal of Chinese Materia Medica 2014;39(4):553-557
Sanqi (Panax notoginseng) is a valuable unique herb, and is also one of the very fast developed varieties of traditional Chinese medicines in recent years with increasing role in traditional Chinese medicine industry. This paper summarized the main experience, industry development and present situation, pointed out the main problems existing in the industry development. On this basis, we put forward the targets and measures for the development of the Sanqi industry in to provide decision-making reference for the sustainable development of the Sanqi industry in China.
China
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Drug Industry
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economics
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trends
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Drugs, Chinese Herbal
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analysis
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economics
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Medicine, Chinese Traditional
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economics
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trends
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Panax notoginseng
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chemistry
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growth & development