Objective To investigate the change of serum prealbumin (PA) and C-reactive protein (CRP) in burn patients and its clinical significance.Methods Sixty eight cases of burn patients were divided into two groups,group Ⅰ [n=38,with total burn surface area less than 15% of total body surface area (TBSA)] and groupⅡ(n=30,with total burn surface area more than 50% of TBSA). Thirty healthy people were set as healthy control group. Particle enhanced immunoturbidimetic assay was applied to detecting the serum levels of PA and CRP for all subjects on the day 1 after burns.Results The CRP level was much higher and serum level of PA was much lower in group Ⅱ than that of group Ⅰ,respectively. There existed significant negative correlation between PA and CRP level.Conclusion The level of serum PA decreases significantly along with total burn surface area expansion. PA can be used as a sensitive marker for estimation of inflammation degree after burns.

Objective To discuss the comparability of ALT, AST, CK and LDH results by ap- plying three different vacuum tubes (no additive tubes, separation gel coagulation promoting tubes and other coagulation promoting tubes) for venous blood samples, and to provide evidence for clinical tests. Methods Fast venous blood from 26 healthy blood donors were collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. Olympus AU5400 Biochemical Analyzer was applied to examining serum ALT, AST, CK and LDH for all pre- pared serum samples. Results There was no statistical difference of serum ALT, AST, CK and LDH level collected in test tubes without additives, separation gel coagulation promoting tubes and other co- agulation promoting tubes. With the no additive tubes as the standard tubes, the above result bias be- tween separation gel coagulation promoting tubes and other coagulation promoting tubes were clinically acceptable. Conclusion There is credible comparability of serum ALT, AST, CK and LDH level a- mong different kinds of venous blood samples collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. The separation gel coagulation promoting tubes and other coagulation promoting tubes can substitute test tubes without additives for ALT, AsT, CK and LDH determination.

Objective: To improve the database of medical equipment archives system and manage medical equipment more scientifically and efficiently. Methods:According to the needs of medical equipment management, the problems of medical equipment archives system would be analyzed and addressed. Results:In recent medical equipment archives system, the database of usage rate, cost-effectiveness, maintenance costs and property management should be improved. Conclusion:The improved archives system could provide reliable basic data and multi-faceted sharing for medical equipment dynamic management.

Objective:To observe the effect of calcitonin gene-related peptide (CGRP) on the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappaB ligand (RANKL) in osteoblast cells through an in vitro breast cancer cell and osteoblast cell co-culture system. Methods:The metastatic breast cancer MDA-MB-231 or MDA-MB-435 cells were co-cultured with osteoblast MG63 cells to establish an in vitro microenvironment of bone metastasis of breast cancer. After treated with CGRP(1?108 mol/L),OPG and RANKL mRNA and protein expressions in osteoblast MG63 cells were examined by RT-PCR and Western blotting. Results:Expression of RANKL in osteoblast MG63 cells was up-regulated at both mRNA and protein levels when osteoblast MG63 cells were co-cultured with breast cancer MDA-MB-231 or MDA-MB-435 cells,while those of OPG in osteoblast MG63 cells were both down-regulated (P

Animals ; Arrestins ; metabolism ; Liver ; pathology ; Liver Cirrhosis, Experimental ; metabolism ; pathology ; Male ; Mice ; Mice, Inbred ICR ; beta-Arrestins

Adult ; Chlorine ; poisoning ; Humans ; Male ; Middle Aged ; Phosgene ; poisoning ; Respiratory Distress Syndrome, Adult ; chemically induced

Aiming at the cultivation goal and requirements as well as the current status of learning and applying medical statistics for medical doctoral students, a new idea of medical statistics teaching for medical doctoral students that combined theory with practice, science research and paper writing was proposed, with respect to improving the teaching content, teaching with clinical cases, enhancing the teaching material development, paying attention to the computer practice, and providing the writing standard for research papers.

Objective To investigate the association between the plasma level of fibrinogen and carotid atherosclerosis(CAS)in patients with ischemic cerebrevascddar disease(ICVD),and the effects intensity of different risk factors on CAS.Methods Carotid intima-media thickness(IMT)was measured by color Doppler uhrasonography in 840 patients with ICVD,including cerebral infarction(ci),transient ischemic attack and insufficient blood-supply of vertebral-basilar artery.According to the results of uhrasonography,the patients were divided into four groups:normal(IMT≤0.9 mm),arterial sclerosis (0.9 mm1.5 mm)and stenosis(stenosis of lumina≥30%).The plasma fibrinogen and other biochemical markers were also detected.The history of hypertension,diabetes,hyperlipemia,smoking,drinking were recorded,and the blood pressure was measured.Results The level of fibrinogen was the highest((3.91±1.05)g/L)in the patients with CI,the difference was significant compared to the patients with TIA((3.45±0.81)S/L,X2=62.812,P<0.01),and so was the prevalence of CAS(77.0%),there was significant difference compared to the patients with TIA(61.8%,X2=9.000,P<0.01).The rate of CAS increased gradually with the quartiles of the fibrinogen(the odds ratios in the 2nd,3rd and 4th quartiles(3.16-3.72 g/L,3.72-4.39 g/L and>4.39 g/L)were 1.80,3.36 and 3.38 respectively,all P≤0.01).There were significant differences of age,fibrinogen,systolic blood pressure,hyperglycaemia and history of diabetes and hypertension between the normal-carotid group and three CAS groups.Multiple logistic regression showed that only the age,fibrinogen and systolic blood pressure significandy influenced on the CAS with the highest risk factor being the fibrinogen.Conclusions In the patients with ICVD,the elevated plasma fibrinogen Was significantly correlated with CAS,and misht play more important role than other traditional risk factors.

OBJECTIVE To investigate the distribution and the resistance of pathogens from the children with VAP in PICU,and to analyze the reasons of antibiotics resistance of the pathogens.METHODS The sputum obtained from the children with final diagnosis of VAP in PICU was cultured and identified from Jan 2005 to Dec 2006.The resistance of the bacteria identified to antibiotics used frequently was determined by Kirby-Bauer method.RESULTS A total of 187 strains were isolated from sputum specimens,of which Gram-negative bacilli and Gram-positive cocci accounted for 76.5% and 23.5%,respectively.Acinetobacter baumannii(17.7%),Escherichia coli(16.0%),Pseudomonas aeruginosa(15.0%) and Klebsiella pneumoniae(13.9%) were the most frequently isolates of Gram-negative bacilli.Their resistant rates to ?-lactam antibiotics were high,especially the ESBLs-producing strains in E.coli and K.pneumoniae.The Staphylococcus epidermidis(5.9%),Staphylococcus aureus(4.8%) and Enterococcus faecalis(4.3%) were the most common strains of Gram-positive cocci.No vancomycin-resistanct strains were found,but resistance rates to ?-lactam antibiotics and other antibiotics were high in S.epidermidis and S.aureus.CONCLUSIONS The main strains cultured from the sputum specimens of children with VAP in PICU are Gram-negative bacilli with high resistance rates to antibiotics,especially the ESBLs producing bacilli to ?-lactam antibiotics.Staphylococcus are the main Gram-positive cocci.

Objective:Atrioventricular nodal reentrant tachycardia(AVNRT) is a common type of arrhythmia,for which radiofrequency ablation(RFCA) is the first therapeutic option.This study is to investigate the causes of 16 cases of temporary atrioventricular block(AVB) during RFCA and 2 cases of permanent AVB after RFCA in common and refractory AVNRT.Methods: We performed RFCA for 77 AVNRT patients by gradually increasing the ablation time,energy and frequency.Generally we chose the lower zone as the first position of ablation and,if invalid,moved the catheter gradually up to the middle or upper zone.Then we analyzed the correlation of AVB with A/V and H-wave in different positions.Results: The AVB risk was increased with the upward movement of the ablation zone and increase of A/V or H-wave(P 0.05). Conclusion: The method of gradually increasing the ablation time,energy and frequency can prevent temporary from permanent AVB.In the common AVNRT group,the middle and lower zone ablation,the multiple-peak A wave,small A wave and big V wave(A/V ≤ 0.45?0.37) with no H wave or H≤0.003?0.01 mV could significantly decrease the risk of RFCA related AVB,but the risk of AVB remains among the refractory AVNRT patients with CSO abnormality or expansion,even if with ablation in the lower zone.