Objective To explore the anti-aging mechanisms of Rhizoma Polygonati Odorati(RPO)and its anti-aging active components.Methods After drenching extracts of RPO for 56 days,the behavioral indexes of memory,learning and tolerance,changes of immune organs of spleen and thymus gland,testis index,plasma superoxide dismutase(SOD) activity,hepatic malondialdehyde(MDA)content were observed in D-galactose-induced suhacute senile mice model. Results Aqueous extract shows a trend of improving the memory and learning abilities,and can increase the tolerance of aging-model mice,prevent the atrophy of thymus gland and testis,enhance the activity of SOD and reduce the content of MDA.Conclusion Aqueous extract of RPO may be the anti-aging active components in RPO,and its mechanism may be concerned with the increase of immune function,prevention of the atrophy of genitical glands,counteraction of oxidation,clearance of free radicals and prevention of senile dementia.

In order to explore the effect of internet of things on the management of cardiac rehabilitation /secondary prevention in patients with myocardial infarction .This article first introduces briefly about the impact and the development of cardiac rehabilita -tion/secondary prevention;then discusss in detail about its current status and limitation between at home and abroad ;meanwhile intro-duces the necessity and prospect of internet of things applied in cardiac rehabilitation /secondary prevention .

Objective To study the value of sentinel lymph node (SLN) for forecasting axillary lymph node metastasis in breast cancer. Methods Fifty-six patients with breast cancer underwent SLN methylene blue mapping and biopsy,as well as axillary lymph node dissection.All lymph nodes received routine HE staining and immunohistochemical examination. Results SLN was successfully identified in 52 cases (52/56,92.8%).Routine pathological examination demonstrated metastasis of SLN in 22 cases and metastasis of non-SLN in 1 case,the false negative rate being 4 3% (1/23).In the remaining 29 cases without pathologically verified metastasis,immunohistochemical staining showed 1 case of CK-19 (+) with EMA (+) and another 1 case of CK-19 (+) with CEA (+),without metastasis of non-SLN. Conclusions SLN methylene blue mapping and biopsy may forecast axillary lymph node metastasis in breast cancer.

Objective To investigate the feasibility of breast conservative surgery for large breast carcinoma(over 3 cm in diameter)after neoadjuvant chemotherapy.Methods A total of 24 patients with breast carcinoma larger than 3 cm in diameter underwent core needle biopsy and neoadjuvant chemotherapy with paclitaxel and/or epirubicin in our hospital from June 2003 to September 2006.The chemotherapy was carried out for 2 to 4 cycles,and then breast conservative surgery was performed in 7 to 10 days.Results After the neoadjuvant chemotherapy,3(12.5%)patients achieved CR,and 21(87.5%)had PR.Pathological CR was detected in 2(8.3%)of the patients.The breast conservative surgery was completed in 14 cases(58.3%),10 patients were converted to modified radical resection.The patients was followed up for 5 to 44 months with a median of 24,during which none of them had recurrence or distant metastasis.Conclusions For patients with large breast carcinoma,breast conservative surgery is feasible after neoadjuvant chemotherapy.

Thyroid cancer is one of the most common malignant tumors in the human endocrine system . It is one of the common diseases in head and neck ,thyroid and breast surgery .Its incidence rate is increasing year by year .With the development of ultrasonography and fine needle aspiration biopsy ,as well as the auxiliary exami-nation of gene detection technology ,the detective rate of early diagnosis of papillary thyroid microcarcinoma ( PT-MC) is getting higher and higher .Compared with conventional surgery , highlighting the advantages of minimally invasive surgery , endoscopic and ultrasound guided percutaneous ablation and other new surgical methods are gradually applied in clinical treatment .Combined with the new guideline ,the present paper reviews the progress in the diagnosis and treatment of papillary thyroid microcarcinoma .

Objective To compare the body fluid count results detected by Sysmex XE-5000 automatic blood cell analyzer and manual method .Methods A total of 300 cases of body fluid specimens (including cerebrospinal fluid and fluid of serous cavity ) were analyzed .RBC ,WBC counting and classification were respectively detected by XE-5000 and manual method of improvement Neubauer counting plate .Results The fresh specimens without contain a large number of cell clusters ,which RBC counts(RBC-BF)(100-10 000)× 106/L ,and WBC counts(WBC-BF) (9-50)× 106/L ,showed there were a linear relationship between the in-strument method(r=0 .998 5 ,0 .986 3) .In the range ,there was no significant difference between XE-5000 and manual method(t=9 .96 ,P>0 .05) .Also in this range the results of instrument correlated with those of manual method(r= 0 .989 3 ,0 .971 7 , 0 .924 9) .For those specimens which contain a large number of cell clusters ,RBC-BF and WBC-BF were a badly linear relationship between the instrument method(r=0 .564 8 ,0 .456 1) .Conclusion Body fluid specimens which are fresh and do not contain a large number of cell clusters ,in the range of RBC-BF (100 -10 000) × 106/L ,WBC-BF (9 -50) × 106/L ,Sysmex XE-5000 automatic blood cell analyzer could ensure the results have good accuracy .

Objective To investigate the characteristic of etiology of chronic cough in Shenzhen.Methods The chronic cough etiology was analyzed in 136 cases with the guidance of cough diagnosis and treatment guidelines(2009 editions)published by Chinese Medical Association.The cough was the main or sole symptom,the duration was no less than 8 weeks and chest X-ray film was normal.Results The causes of chronic cough was confirmed in 125 patients and was not definitely diagnosed in 11 patients by inspection and treatment.Cough due to single cause was found in 104 patients(83.20%,104/125),due to compound causes was found in 21 patients(16.80%,21/125).The first 4 etiologies were cough variant asthma(CVA)with 57 patients(36.31%,57/157),upper airway cough syndrome(UACS)with 41 patients(26.11%,41/157),eosinophilic bronchitis(EB)with 17 patients(10.83%,17/157),occupational injury(including harmful,toxic substances inhalation,etc.)with 10 patients(6.37%,10/157).Conclusions The most common cause of chronic cough in Shenzhen is CVA,UACS,EB.Due to the developed industrialization,there is a lack of understanding the cough course of inhaling more harmful and toxic gases and substances in the manufacturing process.So this should be paid more attention.

OBJECTIVE:To observe the effect of different polarity of components of Rhizoma Polygonati Odovati on the spleen and thymus gland in D-galactose-induced aging-model mice so as to establish the anti-aging mechanism and anti-aging active components of Rhizoma Polygonati Odovati.METHODS:Different polarity of extracts of Rhizoma Polygonati Odovati were extracted respectively with benzin petroleum,acetic ether,dehydrated alcohol,80% ethanol,and water as extract solvents.The model mice were intragastrically administered with different polarity of components of Rhizoma Polygonati Odovati for 56 consecutive days,and the effects of each extractive on the indexes and pathological change of immune organ D-galactose-induced aging-model mice were monitored.RESULTS:Compared with each other extractive,aqueous extract of Rhizoma Polygonati Odovati can prevent the atrophy of thymus and spleen,and ameliorate the patho-constitution of spleen and thymus gland.CONCLUSION:Aqueous extract of Rhizoma Polygonati Odovati maybe the anti-aging active components in Rhizoma Polygonati Odovati,and its mechanism maybe related to its function to prevent the atrophy of immune organs and improve the immune function.

BACKGROUND: Cervical lymph nodes are draining region of cornea. It is believed that aqueous fluid goes through a minor pathway named uveoscleral drainage, which will allow passage of antigen-presenting cells (APC) directly to the draining lymph nodes and induce allograft rejection after keratoplasty.OBJECTIVE: To explore the inhibitory effects of cervical lymphadenectomy in alkali induced high-risk corneal transplantation.DESIGN: A randomized controlled animal experiment.SETTING: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University.MATERIALS: The experiment was performed in the State Key Laboratory of Ophthalmology (No. 2006DA105054), Zhongshan Ophthalmic Center, Sun Yat-sen University from May 2005 to February 2007. 144 male animals (1-2 months old) including 104 SD rats and 40 Wistar rats were provided by the animal experimental center of Sun Yat-sen University. Sandwich enzyme-linked immunosorbent assay (ELISA) kits for interleukin-2 (IL-2) and interferon-γ (IFN-γ) were brought from BioSource International company (USA). The animal treatment in the experiment was accorded with the statement in Association for Research in Vision and Ophthalmology (ARVO) for animals.METHODS: With the SD rats as recipients, and Wistar rats as donors, all rats were subjected to corneal allografting. The recipient rats were randomly divided into 4 groups (n=20): group A (control group) which underwent corneal transplantation; group B which was subjected to bilateral cervical lymphadenectomy; group C, corneal transplantation 21 days after the alkali burn injury; group D, cervical lymphadenectomy following group C. The immune rejection of grafts was evaluated by detecting the expression of IFN-γ and IL-2 using ELISA. The time when allograft rejection occurred was recorded and mean survival time (MST) was compared among the groups. The development of corneal inflammation and new vessels was examined by slit lamp microscope and histopathological examination.MAIN OUTCOME MEASURES: ①The development of corneal inflammation after corneal alkaline burns. ②MST of rats in each group following transplant. ③The expression of IL-2 and IFN-γ in grafts of each group. RESULTS: ①Normal rat cornea was transparent without inflammation or neovascularization. There were many inflammatory cells invading to stroma on day 3 after burn. Then, the inflammation of cornea resolved gradually 3 weeks after the burn, but corneal neovascularization reached the peak at that time. Corneal blood vessels regressed completely at the end of 8 weeks after the burn. ②The MST of group A, B, C, and D was (10.40±1.14), (46.30±9.46), (7.00±1.58), and (15.00±3.39) days, respectively. Compared with the group A, the MST of group B was significantly longer (P < 0.05), and the MST of grafts in group D was also significantly longer than group C (P < 0.05). ③The expression of IFN-γ and IL-2 proteins was absent in group B. Compared with group C, the expression of IL-2 and IFN-γ proteins in group D significantly decreased on days 3, 7, 10, and 14 after keratoplasty (P < 0.05). CONCLUSION: Cervical lymphadenectomy therapy can effectively inhibit corneal allograft rejection in normal and high-risk corneal beds after alkali burn injury.

A highly sensitive immuno-PCR assay based on sandwich ELISA and PCR was developed to detect the circulating antigen in trichinellosis. Antigens were purified from the muscle larvae of Trichinella spiralis, and the myeloma cells were fused with spenocytes immunized with T. spiralis antigens to product the specific monoclonal antibodies. Indirect ELISA was used to select the antibody-secreting hybrodoma cells. By this method of procedure, monoclonal antibody F4C6 against the T. spiralis ES antigen was obtained, which was used as the indicator antibody, while the rabbit polyclonal antibodies against T. spiralis were to be used as capturing antibodies. The plasmid Bluecript Ⅱ KS was amplified by PCR with biotin-labeled primer M13-20, and thus the biotin-labeled DNA was obtained. Both the second antibody and DNA labeled with biotin were to be linked with 100 ng/ml avidin. The whole procedures of assay consisted of two steps, in which the circulating antigens were captured by monoclonal antibody through sandwich ELISA in the first step, and the DNA linked by monoclonal antibody was amplified by PCR in the second step. The sensitivity of this method was compared with that of the ELISA assay. It was found that the measuring ranges to detect the circulating antigens in trichinellosis were 50 pg/L to 0.005 pg/L for the immuno-PCR assay, and 5 μg/L to 0.05 μg/L for ELISA assay, the former was quite higher than that of the latter. It is evident that this method is highly sensitive for the detection of circulating antigens in trichinellosis.