1.Development of medical science and technology and doctor-patient relationship
Jie WANG ; Rui PAN ; Hui HUANG
Chinese Journal of Hospital Administration 1998;0(11):-
The rapid development of medical science and technology, which has greatly promoted the development of medicine and the improvement of diagnosis and treatment, has also brought about various negative impacts in clinical practice on the doctor-patient relationship. The paper attempts to analyze these impacts from such perspectives as medical treatment, society, ethics, and law and proposes some corresponding countermeasures in perspective of the present reality: (1)rationally controlling medical expenses; (2) effectively stepping up communication; (3)appropriately handling difficult ethical problems; (4) amplifying necessary health rules and regulations. The goal is to create a win-win situation for both progress in medical science and technology and improvement of the doctor-patient relationship.
3.A cell membrane chromatography method for screening 5-HT receptor agonists from drug pair of Chuanxiong Rhizoma and Angelicae Dahuricae Radix.
Hui DU ; Nan LV ; Jie HUANG ; Mei DENG
China Journal of Chinese Materia Medica 2015;40(3):490-494
Migraine is one of the common and frequently encountered diseases. The study proves that 5-hydroxytryptamine (5-HT) receptor, plays an important role in the occurrence of migraine. Rat striatum was used for preparation of the cell membrane stationary phase (CMSP) in our experiments. The cell membrane chromatography (CMC)-offline-HPLC system was applied to specifically recognize the components from the drug pair of Chuanxiong Rhizoma and Angelicae Dahuricae Radix, which interact with the receptors on CMSP. The dissociation equilibrium constant (KD) was measured in a rat striatum/CMC system, performed by continuously pumping sumatriptan, a 5-HT1D agonist, ranging from 2.42 x 10(-8) to 4.84 x 10(-7) mol · L(-1) through a CMC column, and the capacity factors (k') were recorded. The KD value obtained from the model was (4.59 ± 0.33) x 10(-6) mol · L(-1) for imperatorin, and the rat model of migraine induced by nitroglycerin was applied to validate the pharmacological effects of the drug pair. The results indicated that the CMC method could be a quick and efficient way for characterizing the drug-receptor interactions in vitro.
Angelica
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chemistry
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Animals
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Cell Membrane
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chemistry
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Chromatography, High Pressure Liquid
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methods
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Drug Evaluation, Preclinical
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methods
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Drugs, Chinese Herbal
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chemistry
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Male
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Migraine Disorders
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drug therapy
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Rats
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Rats, Sprague-Dawley
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Receptor, Serotonin, 5-HT1D
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chemistry
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Serotonin Receptor Agonists
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analysis
4.Strengthen the management of clinical research and promote the good development of clinical research
Yanqin ZOU ; Hui HUANG ; Jie DAI ; Guiping LIN
Chinese Journal of Medical Science Research Management 2013;26(5):293-295
In China,with the development of medicine,more and more clinical researches are increased.However,along with this development,there are still a lot of problems.In order to normalize the management of clinical researches,make the research results reliable and benefit for patients,several strict aspects of management are taken by our hospital,included standardizing the application procedures,introducing the roles of the academic committee and the ethics committee,strengthening the projects management and researchers training,etc.And all these ways promote the good development of clinical researches of our hospital.
5.Diallyl disulfide induces human leukemia HL-60 cells differentiation by up-regulating the expressions of p21,STAT1 and CAMTA1
Weiguo HUANG ; Hui TAN ; Lan YI ; Jie HE ; Qi SU
Chinese Pharmacological Bulletin 2010;26(4):513-516
Aim To investigate the molecular mechanisms of differentiation in human leukemia HL-60 cells induced by diallyl disulfide(DADS)using suppression subtractive hybridization(SSH).Methods In our privious study,the subtractive cDNA library was constructed successfully and efficiently. 30 clones were randomly analyed with restriction enzyme.The inserts of cDNAs were analyzed by restrictive enzyme EcoR I.Positive clones were sequenced and the homology of resulting cDNA sequences were analyzed through bioinformatics software Blastn.Results 18 clones contained 100~600 bp cDNA inserts.10 differantiation genes were obtained and involved in cell cycle,signal transduction,metabolism and RNA binding.And 3 of 10 genes,p21,STAT1 and CAMTA1 were up-regulated and detected by RT-PCR,the results matched with SSH.Conclusion sThere are tight correlation between the differentiation induced by DADS and three-upregulated gene:p21,STAT1 and CAMTA1.
6.Mechanism underlying intrauterine growth retardation induced by caffeine and its research advance
Shu ZHOU ; Jing HUANG ; Chong BAO ; Jie PING ; Hui WANG
Chinese Journal of Pharmacology and Toxicology 2010;24(1):77-80
Intrauterine growth retardation (IUGR) is one of the most commonly encountered developmental toxicity, which could lead to perinatal morbidity and mortality, be also extended from the fetus to adulthood, and seriously affect the quality of the population. Caffeine widely exists in a variety of daily beverages and some drugs. Its consumption is increasing year by year. Caffeine intake during pregnancy is one of the risk factors for IUGR. However, its mechanism of adverse outcome based on embryonic research is still unclear. In this paper, the possible mechanisms of caffeine-induced IUGR focusing on 3 important factors-the mother, placenta and fetus were explored. Caffeine's impact on the mother is the chronic activation of renin-angiotensin system; on the placenta, caffeine induces cell damage or the failure of the cell proliferation/apoptosis balance, leading to blockage of blood supply to the placenta; caffeine is also capable of directly affecting fetal development through interfering its neuroendocrine.
7.Use of self-made drainage device in surgical procedure for prolapse and hemorrhoid operation
Hui YE ; Shujuan HUANG ; Zhilin GONG ; Qichang ZHOU ; Jie YU
Chinese Journal of General Practitioners 2013;12(8):641-642
A total of 120 patients with Ⅲ/Ⅳ degree hemorrhoids were randomly divided into 3 groups.Self-made drainage(A group),traditional device(B group)and stuffing(C group) were used at the end of operation.The patient postoperative complications and satisfaction were observed and compared.The results showed that postoperative abdominal distension,pain,medical dressing dampness and satisfaction were better in A group than those in B and C groups(all P <0.01).Postoperative abdominal distention in B group was lighter than that in C group and medical dressing dampness less in C group than that in B group (all P < 0.01).Thus the self-made drainage device may reduce the levels of postoperative abdominal distension,pain and medical dressing dampness and increase postoperative patient satisfaction.
8.Pondering on the Acceleration of High-Tech Medicine Industry Development
Rui PAN ; Hui HUANG ; Jie WANG ; Jianhua LU ; Jianguo WU
China Pharmacy 2001;0(07):-
OBLECTIVE:To promote a sustained and quick development of the high-tech medicine industry in our country.METHODS:The related literature was consulted and the current developmental status of the high-tech medicine in-dustry in our country was analyzed.RESULTS&CONCLUSIONS:The high-tech industry in our country can be pushed on only through rearrange industry mix at the right time,constructing a serial comprehensive development terrace,supporting es-pecially those medicine enterprises that with core technology and independent intellectual property rights,building a virtual strategic league together with the academy of higher learning and scientific research institutes.
9.Induction of differentiation by diallyl disulfide through inhibition of JAK1/STAT3 in human leukemia HL-60 cells
Minghua WU ; Weiguo HUANG ; Hui TAN ; Jie HE ; Qi SU
Chinese Pharmacological Bulletin 1986;0(05):-
Aim To investigate JAKs/STATs signal transduction change in HL-60 cells differentiation induced by diallyl disulfide(DADS)and molecular mechanism regulating the differentiation.Methods After incubation of HL-60 cells with DADS or AG490(50 ?mol?L -1),the cell differentiation indexes were observed by cytomorphology, NBT reduction ability assay,cell myeloid differentiation antigen CD11b by flow cytometry. Kinase activity of JAKs/STATs was tested by western-blotting and expressions of nucleus transcription genes stats,c-myc,c-fos,c-jun were detected by immumocyte chemistry method.Results Cell differentiation index changes indicated that HL-60 cells were induced differentiation toward granulocytic lineage by DADS, Western blot test demonstrated that constitive phosphorylation of Jak1,stat3 kinase was suppressed. Stat3,c-myc gene expression decreased and c-fos, c-jun gene expression increased in HL-60 cells treated with DADS through immunocyte chemistry.Conclusion Inhibition of phosphative Jak1, Stat3 was involved in HL-60 cells differentiation induced by DADS, its molecular mechanism might be related to modulation of gene expression associated proliferation and differentiation,and inhibition of DNA systhesis, induction differentiation.
10.Protective Value of Low-dose CT Scanning in Temporal Bone of Children
Nanzhou WU ; Zhengyang XU ; Xiangbing BIAN ; Hui HUANG ; Jie YANG
Chinese Medical Equipment Journal 2003;0(10):-
0.05) . Conclusion An acceptable image quality can be achieved for pediatric patients by reducing the mA value to 40 to 80mA used for conventional temporal bone, and the low dose CT scanning ought to be extended in the temporal bone decease for children.