Child ; Erythropoietin ; adverse effects ; Humans ; Male ; Recombinant Proteins ; Red-Cell Aplasia, Pure ; chemically induced

Objective:To study the effect of compressive stress on expression of connective tissue growth factor(CTGF) in rabbit mandibular condylar chondrocytes in vitro. Methods:CTGF mRNA were determined by semiquantitative RT-PCR in rabbit mandibular condylar chondrocytes cultured in vitro. Results:Within a certain compressive stress, CTGF mRNA were up-regulated with the increase of compressive stress. While under certain compressive stress, CTGF mRNA were gradually up-regulated following the increase of culturing time, and reaching the maximum at 2~6 h, then gradually down-regulated until at 24 h. Conclusion:Application of different compressive stresses can up-regulate the expression of CTGF mRNA in rabbit mandibular condylar chondrocytes. It may be involved in the stress -mediated mandibular condylar cartilage remodeling.

Animals ; Basic Helix-Loop-Helix Transcription Factors ; metabolism ; physiology ; Cadherins ; metabolism ; Cell Transformation, Neoplastic ; metabolism ; pathology ; Epithelial Cells ; pathology ; Homeodomain Proteins ; metabolism ; physiology ; Humans ; Mesenchymal Stromal Cells ; pathology ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasms ; metabolism ; pathology ; Snail Family Transcription Factors ; Transcription Factors ; metabolism ; physiology ; Zinc Finger E-box-Binding Homeobox 1

Objective To establish a method of high resolution melting analysis (HRM) with unlabeled probe for detection of rt204 mutation in HBV P gene.Methods Plasmids with wild strain rt204M,mutant strains rt204I and rt204V were constructed,and the probes were designed and optimized.HRM plots were established by the constructed plasmids.A total of 185 samples were collected from patients with chronic hepatitis B in the Affiliated Hospital of School of Medicine of Ningbo University during May 2010 and May 2012.All samples were detected by HRM,and matched with characteristic melting pattern.rt204 mutations were screened,and then verified by DNA sequencing.Paired x2 test was used for the comparison of the detection of mutations.Results The melting temperatures for rt204I,rt204V and rt204M were 58.0℃,60.6℃ and 62.5℃,respectively.Among 185 samples,168 samples (90.8％) could be analyzed by HRM method,and 155 samples (83.8％) coule be successfully sequenced (P ＜0.01).In the 155 samples which were completely analyzed by HRM assay and sequencing,75 samples were rt204M,55samples were rt204I,and 25 samples were rt204V by using HRM method,with an overall mutation detection rate of 51.6％ (80/155) ; and by sequencing,110 samples were rt204M,30 samples were rt204I,and 15samples were rt204V,with an overall mutation detection rate of 29.0％ (45/155).The difference onmutation rates detected by the above two methods was of statistical significance (P ＜ 0.01).Conclusion HRM with unlabeled probe is simple,sensitive,rapid and specific for detection of rt204 mutation in HBV P gene.

Adenocarcinoma, Mucinous ; pathology ; Breast Neoplasms ; pathology ; Carcinoma, Ductal, Breast ; pathology ; Carcinoma, Renal Cell ; pathology ; Carcinoma, Squamous Cell ; pathology ; Cystadenocarcinoma, Mucinous ; pathology ; Cystadenoma, Mucinous ; pathology ; Female ; Giant Cells ; pathology ; Humans ; Osteoclasts ; pathology ; Ovarian Neoplasms ; pathology ; Pancreatic Neoplasms ; pathology ; Thyroid Carcinoma, Anaplastic ; Thyroid Neoplasms ; pathology ; Tongue Neoplasms ; pathology ; Urologic Neoplasms ; pathology

Antimony ; blood ; Humans ; Spectrophotometry, Atomic ; methods

ObjectiveTo study the therapeutic efficacy of lamivudine in preventing the activity of hepatitis B virus in patients with hepatocellular carcinoma and hepatitis B virus infection. MethodsFrom March 1999 to March 2000,72patients with hepatocellular carcinoma and hepatitis B virus infection were divided into 2 groups in complete randomization. In group 1,38 patients were treated with lamivudine 100mg/qd during perioperative period. Before and after operation,we detected HBV DNA with assay of quantitative polymerase chain reaction (QPCR). The control group(2) wasn't given anti-hepatitis B virus treatment. ResultsThe results showed that in group 2,the copies of HBV DNA in sera one week,two weeks after operations were significantly higher than those before the operation(P<0.01);in group 1,the copies of HBV DNA in sera one week,two weeks after operations were significantly lower than those before operation(P<0.01),all within the safety threshold;between group 1 and group 2,the indexes of ALT in sera and Child status didn't significantly change before and after operation.The postoperative complications were significantly different two weeks after operation.Conclusion It suggests that surgical interventions may result in promoting the process of HBV replication,the activity of HBV infection should be treated in perioperation, and the replication of HBV can be inhibited by lamivudine safely to the safety threshold within a short time, though lamivudine doesn't improve the liver biochemistry significantly.

Child ; China ; Communicable Diseases ; Communicable Diseases, Emerging ; Humans ; Multicenter Studies as Topic

Cancer metastasis is the most dangerous stage of tumorigenesis and evolution, the primary cause of death in cancer patients. Clinically, more than 60% of cancer patients have found metastasis at the time of examination. Modern medicine has made significant progress on the mechanisms of cancer metastasis in recent years, from the simple "anatomy and machinery" theory forward to the "seed and soil" theory, then to the "microenvironmental" theory and the "cancer stem cell" theory. The emerging "cancer stem cell" theory successfully explains phenomenon such as tumor genetic heterogeneity, anoikis resistance, tumor dormancy, providing more new targets and ideas for the diagnosis and treatment of cancer metastasis.
Animals ; Humans ; Medicine ; methods ; Neoplasm Metastasis ; Neoplasms ; drug therapy ; pathology

Objective To evaluate the role of mierosurgery in surgical treatment to urethrocutaneous fistula after urethroplasty in hypospadias and improve surgical results.Methods From 1999 to 2006,44 urethrocutaneous fistulae (more than 3mm in diameter) after urethroplasty for hypospadias in 33 patients were repaired with different skin flaps.For example,Thiersch technique,urthroplasty,etc.Microsurgical tech- nique was employed in every case.Results The success rates of different procedure were 84.8% (28/33) for Thiersch technique,100% (11/11) for urethroplasty respectively.The total success rate was 88.6%(39/ 44).Conclusion It's just application of skin flap for repairing of big or complex urinary fistula after hypos- padias surgery.The application of microsurgical technique can increase success rate.It is necessary to excise scar and partial urethra for hypospadias fistula combined with urethral structures,cicatricial eontracture and in- curvation of penis.Rich blood-supply,low tension and atraumatic technique are all very important to improve surgery success rates of urinary,fistula after hypospadias repair.