Objective To quantitatively compare the effects of bleach and peracetic acid reprocessing on the clearance and surface charge characteristics of Fresenius F80B polysulfone dialyzers. Methods Clearance experiments were performed using urea, vitamin B_12, and polydisperse dextrans in an in vitro dialysis circuit. Clearance, ultrafiltration coefficient and zeta potential were obtained on a new F80B dialyzer, after exposure to plasma in a 3 h in vitro dialysis session, and after cleaning with bleach and peracetic acid.Results Bleach was able to remove the protein deposit, restoring the clearance characteristics, but there was a significant increase in the net negative charge of the membrane due to chemical reaction with the bleach. In addition, longer time exposure to bleach altered the membrane transport characteristics, increasing the solute clearance. Dialyzers cleaned with peracetic acid had significantly lower clearance of the larger dextrans due to the presence of residual protein on or within the membrane. Conclusion Cleaning with bleach and peracetic acid may have dramatically different effects on the clearance and surface charge characteristics of F80B polysulfone dialyzers.

Objective: To study the chemical constituents of Huoxiang Zhengqi Liquid. Methods: Silica gel column chromatography, preparative thin-layer chromatography, and recrystallization were used to isolate the chemical constituents from chloroform extract. And the structures of compounds were identified by spectral analysis and physicochemical properties. Results: Fifteen compounds were obtained and identified as liquiritin (1), liquiritigenin (2), isoliquiritigenin (3), formononetin (4), oxypeucedanin hydrate (5), byakangelicin (6), hesperidin (7), 5, 7, 8, 3′, 4′- pentamethoxyflavone (8), 5, 6, 7, 3′, 4′-pentamethoxyflavone (9), 5, 7, 8, 4′-tetramethoxyflavone (10), nobiletin (11), 3, 5, 6, 7, 8, 3′, 4′-heptamethoxyflavone (12), tangeretin (13), honokiol (14), and magnolol (15). Conclusion: All the compounds are isolated from Huoxiang Zhengqi Liquid for the first time. Compounds 1-4 may come from Glycyrrhizae Radix et Rhizoma; compounds 5 and 6 from Angelicae Dahuricae Radix; compounds 7-13 from Citri Reticulatae Pericarpium; and compounds 14 and 15 from Magnoliae Officinalis Cortex.

BACKGROUND:Traditional Chinese medicine plays a specific role in the clinical diagnosis and treatment of ischemia reperfusion injury. OBJECTIVE:To investigate the effect of Bu Yang Huan Wu Decoction on ischemia reperfusion injury in rats after replantation of of lower limbs, and evaluate the protective effect and mechanism of ischemic tissue. METHODS:A total of 120 Wistar rats were randomly divided into four groups:normal control (intragastric administration of saline after anesthesia), Bu Yang Huan Wu Decoction (intragastric administration of 10 mL/kg Bu Yang Huan Wu Decoction immediately after reperfusion), edaravone (intravenous administration of 10 mL/kg edaravone immediately after reperfusion), and blank control (intragastric administration of saline) groups. At 24 hours after reperfusion, the myeloperoxidase, lactate dehydrogenase, nitricoxide synthase, inducible nitricoxide synthase, constitutive nitricoxide synthase level in the blood were detected. At 1 week after reperfusion, the activity of superoxide dismutase in gastrocnemius tissue, nitrogen monoxidum and malondialdehyde, and the load of Ca2+were assayed. RT-PCR semiquantitative analysis was performed to detect bcl-2 mRNA and bax mRNA transcriptional levels in the gastrocnemius.RESULTS AND CONCLUSION:Bu Yang Huan Wu Decoction protected the rats from ischemia reperfusion injury by reducing the production of oxygen free radicals, reinforcing the activity of superoxide dismutase, lessening the calcium overload, decreasing the production of nitrogen monoxidum, up-regulating the expression of anti-apoptotic gene bcl-2, down-regulating the expression of pro-apoptotic gene bax, and attenuating the injury of skeletal muscle after ischemia reperfusion injury. Bu Yang Huan Wu Decoction has significant protection on cerebral ischemia reperfusion injury in rats after replantation of severed limb, which may be related to the scavenge of free radicals, inhibition of skeletal cells apoptosis, and attenuation of reperfusion injury.

OBJECTIVE:To observe the clinical short-term efficacy and safety of brucea javanica oil injection combined with oxaliplatin and xeloda in the treatment of elderly patients with advanced gastric cancer. METHODS:82 elderly patients with ad-vanced gastric cancer were randomly divided into control group and observation group. Control group was treated with Oxaliplatin injection 130 mg/m2 added into 5% Glucose injection 250 ml for 2 h,iv,d1+Xeloda tablets 1 000 mg/m2,orally,twice a day, d1-14;based on the treatment of control group,observation group was additionally treated with 10% Brucea javanica oil injection 30 ml added into 0.9% Sodium chloride injection 250 ml,iv,once a day,d1-14. 21 days were as a treatment course,and it lasted 3 courses. The short-term efficacy,life quality and toxicity reactions were evaluated in 2 groups. RESULTS:The short-term efficacy in observation group was significantly higher than control group,life quality was significantly better than control group,and the in-cidences of leucopenia and liver damage were significantly lower than control group(P<0.05). CONCLUSIONS:Brucea javanica oil injection combined with oxaliplatin and xeloda has better efficacy than oxaliplatin combined with xeloda in the treatment of elder-ly patients with advanced gastric cancer,with good safety.

OBJECTIVE: To evaluate the anticoagulation therapy regimen in one patient with dilated cardiomyopathy and severe heart failure. METHODS: Literatures and clinical practice guidelines were consulted, referring to the management of anticoagulation therapy in heart failure with sinus rhythm. RESULTS: Clinical pharmacist participated in the process of drug treatment and decided on optimal anticoagulation therapy. CONCLUSION: Individual bleeding and thrombotic risk should be evaluated to optimize the treatment of individual patients with heart failure.

Asphyxia Neonatorum ; therapy ; Humans ; Infant, Newborn ; Resuscitation ; methods

Objective To evaluate the role of bispectral index (BIS) in assessing the depth of sedation in hypogastric operation of children. Methods Forty children undergoing hypogastric operation were divided into two groups with 20 cases each by pre-school (age 3-5) (Ⅰ group) and school (age 6-12)(Ⅱ group). Sedation was solely maintained with TCI propofol. The infusion was adjusted till adequate scdation (OAA/S≤ 1 score) before sacral block. The course of propofol infusion and BIS of recovery period were closely observed. Sedation scores were assessed by special anesthetists. Results During recovery period, the mean values of BIS were gradually increased, when BIS > 76 predicted 94% sensitivity and 50% specificity in Ⅰ group, 100% sensitivity and 100% specificity in Ⅱ group. ROC area under the curve of the two groups were 0.917 ± 0.007, 0.955 ± 0.004. Conclusions BIS can predict fairly well the level of consciences during recovering periods. BIS monitoring is an effective and reliable method to guide children recovery.

Cystadenocarcinoma, Mucinous ; pathology ; surgery ; Cystadenocarcinoma, Serous ; pathology ; surgery ; Female ; Humans ; Lymphatic Metastasis ; Neoplasm Invasiveness ; Ovarian Neoplasms ; pathology ; surgery ; Ovariectomy ; methods ; Prognosis

Objective To study the effect and safety of compound Biejiaruangan tablets combined with entecavir on the treatment of viral hepatitis B cirrhosis.Methods100 cases with hepatitis B cirrhosis in Zhejiang Taizhou hospital from June 2014 to December 2016 were selected as the research object in this study which were randomly divided into the control group and the experimental group 50 cases in each group.The control group were given entecavir, the experimental group were given compound Biejiaruangan tablets combined with entecavir.The clinical effect and the adverse reaction in the two groups were compared.ResultsThe effective rate was 90.0% in the experimental group, which was significantly higher than 72.0% in the control group(P<0.05).There were 2 patients vomiting in the experimental group, the adverse reaction rate was 4.0%.There were 9 cases of vomiting and headache in the control group, the adverse reaction rate was 18.0%.T,he adverse reaction rate(in the experimental group was significantly lower than that in the control group(P<0.05).ConclusionThe clinical effect of compound Biejiaruangan tablets combined with entecavir on the treatment of viral hepatitis B cirrhosis is better, which can reduce the rate of adverse reactions in a certain extent, is high safety, and worth popularizing.

Objective To investigate the correlation of blood lipid metabolism abnormity and other components of metabolic syndrome on angiocardiopathy and cerebrovascular diseases in the elderly people. Methods The clinical data of elderly people, who underwent medical examination during 1996 to 2005 in the General Hospital of PLA, were retrospectively analyzed. Based on the analysis, the co-existence of blood lipid disorders with glucose metabolic abnormity, hypertension and obesity, as well as their influence on cardio-cerebrovascular diseases were further analyzed. Results The prevalence of blood lipid metabolic disturbance was 69.6%, in which the prevalence of hyper-cholesteremia and hyper-triglyceride were 23.6% and 21.7%, respectively. The prevalence of hyper-cholesteremia accompanied by hyper-triglyceride (mixed group) was 19.3%, that of hyper-triglyceride with lower high density lipoprotein cholesterol was 5.03%, while it was 30.4% in the normal group. There was a higher risk of having the above abnormal condition in patients with type 2 diabetes mellitus (T2DM) and obesity, and they were more frequently complicated by hypertension in the hyper-triglyceride group (70.8%). When the patients with lipid metabolic disturbance were complicated by T2DM, hypertension and obesity, the prevalence of cardiovascular or cerebrovascular diseases increased. The prevalence of CHD was higher in the patients with hyper-cholesteremia and hypertension or diabetes, and incidence of both CHD and CVD was higher in the patients with hyper-triglyceride and either one of hypertension, diabetes or obesity. Conclusion There is high prevalence of lipid metabolic disturbance (more than two thirds) and other components of metabolic syndrome, and high risk of suffering from cardiovascular or cerebrovascular diseases in elderly people. The aging patients with hyper-triglyceride are vulnerable to cardiovascular or cerebrovascular diseases, and more attention should be paid in clinical practice in future.