1.Therapeutic Observation of Acupuncture-moxibustion for Urinary Retention after Anorectal Operation
Shanghai Journal of Acupuncture and Moxibustion 2017;36(3):303-307
Objective To observe the clinical efficacy of acupuncture-moxibustion in treating urinary retention after anorectal operation.Method Forty patients with urinary retention after anorectal operation were randomly allocated to a treatment group and a control group, 20 cases each. The treatment group was intervened by acupuncture -moxibustion, and the control group by oral administration of Pyridostigmine bromide tablets. For the two groups, 10 d treatments were taken as a course. Two treatment courses later, the residual urine volume and spontaneous micturition were measured to see the changes, and the clinical efficacies were also compared. Result The total effective rate was 90.0% in the treatment group versus 70.0% in the control group, and the between-group difference was statistically significant (P<0.05). The residual urine volume was significantly changed after the treatment in both groups (P<0.05). After the intervention, the residual urine volume in the treatment group was significantly different from that in the control group (P<0.05). After the treatment, the mean spontaneous micturition time was (16.52±3.18)min in the treatment group, versus (41.37±5.63)min in the control group, and the between-group difference was statistically significant (P<0.05).Conclusion Acupuncture-moxibustion is an effective approach in treating urinary retention after anorectal operation, since it can reduce the residual urine volume and shorten the spontaneous micturition duration.
2. Relation between ionization and the expressions of HIF-1α and VEGF in anoxia condition
Tumor 2007;27(7):545-548
Objective: To investigate the effect of ionization on the expression of HIF-1 α and VEGF in anoxia condition, in order to search an effective method for improving the radiosensitivity. Methods: HepG2 hepatoma carcinoma cells were divided into four groups: the control group; the hypoxia group; the radiation group; the hypoxia and radiation group. The cell apoptosis was detected by fluorescence microscope. The cell viability was analyzed by MTY method. And we measured the expressions of HIF-1 α mRNA and VEGF mRNA by RT-PCR. Results: Cell apoptosis: no apoptosis was observed in the control group; few apoptosis occurred in the hypoxia group; bulk apoptosis was observed in the radiation group; less apoptosis occurred in the hypoxia and radiation group than that in the hypoxia group. The order of surviving fraction was the control group > the hypoxic group > the hypoxia plus radiation group > the radiation group; The order of the expression of HIF-1 α mRNA: the hypoxia plus radiation group > the hypoxic group > the radiation group, no significant difference was observed between the radiation group and the control group; The order of the expression of VEGF mRNA: the hypoxia plus radiation group > the hypoxic group > the radiation group > the control group. Conclusion: The expression of HIF-1α induced by hypoxia can prevent hepatoma cells from the damage of radiation. The HIF-1 α decreases the radiosensitivity through inducing VEGF expression in the HepG2 cell line.
3.Role and Clinical Significance of Myeloid Differentiation Protein-2 in Endotoxin Signal Transduction
xi-hua, YUAN ; chang-hui, CHEN
Journal of Applied Clinical Pediatrics 1992;0(06):-
Myeloid differentiation protein-2(MD-2)can separately and simultaneously bind lipopolysaccharide(LPS)and toll-like receptor 4(TLR4)has been shown to play critical roles in mediated recognition responses to LPS by TLR4 and signal transduction induced by LPS.MD-2 can be bound by LPS,not TLR4.The cells have no responsiveness or weak responsiveness to LPS without MD-2.MD-2 can be secreted into blood plasma,formed soluble MD-2 and remotely regulated cells that contained TLR4 without MD-2.MD-2 has been shown to play important roles in endotoxin signal transduction.MD-2 is a small molecular,short nucleic acid fragment,easily regulated should become a new potential anti-inflammatory target.
4.The role of S100A4 in cancer cells and its potential application in the search for new treatment targets
Bulletin of The Academy of Military Medical Sciences 2010;34(1):88-91
S100A4, a member of S100 superfamily of Ca~(2+)-binding proteins, is a polypeptide containing 101 amino acids. S100A4, which is overexpressed in most tumor cells, plays pivotal roles in growth, invasion and migration of tumor by regulating cell cycle progression, differentiation, apoptosis, invasion and metastasis of cells. This article reviews S100A4′s structure, function and potential application as the new target in the therapy of tumors.
5.Influence of Brain Hypoxia-Ischemia on Expression of Glucose Transporter 1 Genes and Glucose Transpsorter 3 Genes in Neonatal Rats
zheng, CHEN ; hui-jin, CHEN ; ming-hua, JIANG ; long-hua, QIAN ; guan-yi, CHEN
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To understand the mechanism of cerebral energy failure after hypoxia ischemia at the molecular level and to establish the protocol for the safe and effective treatment of hypoxic-ischemic encephalopathy(HIE).Methods One hundred neonatal rats were divided into normal control group and hypoxic-ischemic(HI) group. SD rats of both groups were decapitated at the time of 2 h,24 h,48 h,72 h and 7 d after HI.These tissues of cerebrum,cortex and hippocampus were taken out to explore the influence of HI on the expression of GLUT1 and GLUT3 genes with the method of RT-PCR.Results There was an enhancement in the expression of GLUT1 and GLUT3 genes with the increasing of day age. The expression was more intense in hippocampus than that in cortex. However, HI could significantly enhance the expression of GLUT genes. The expression was higher in cortex than that in hippocampus. The expression of two genes reached the peak at 24 h after HI, but was significantly lower than that in control group at 7 d after HI.Conclusion The increased expression of GLUT genes can maintain the energy supplement for the brain and delay a cascade reaction of cerebral energy failure.
7.Ultrasound-guided double-tract percutaneous cholecystostomy combined with choledochoscopic gallbladder-preserving cholecystolithotomy for elderly patients with acute calculous cholecystitis
Hua WANG ; Qi CHEN ; Tao WANG ; Lijun TANG ; Hui ZHANG
Chinese Journal of Digestive Surgery 2015;14(2):149-151
Objective To investigate the clinical efficacy of ultrasound-guided double-tract percutaneous cholecystostomy combined with choledochoscopic gallbladder-preserving cholecystolithotomy for the elderly patients with acute calculous cholecystitis.Methods The clinical data of 35 elderly patients with cholecystolithiasis who were admitted to the General Hospital of Chengdu Military Command from January 2012 to December 2013 were retrospectively analyzed.All the 35 patients received ultrasound-guided double-tract percutaneous drainage,and then received choledochoscopic gallbladder-preserving cholecystolithotomy.The long-term oral use of anti-inflammatory and cholagogue medications after operation reduced recurrence of cholecystolithiasis.All the patients were followed up by telephone interview and outpatient examination till June 30,2014.Results All the 35 patients undergoing successfully the ultrasound-guided double-tract percutaneous cholecystostomy.Of the 35 patients,1 patient with the puncture bleeding was cured successfully by symptomatic treatment,1 patient was treated by cholecystectomy due to the unformed sinus tract under costal margin,and other 34 patients underwent successfully cholecystolithotomy.The follow-up was carried out for 4 to 24 months and 1 patient was diagnosed as with recurrence of cholecystolithiasic.Conclusion Ultrasound-guided double-tract percutaneous cholecystostomy combined with choledochoscopic gallbladder-preserving cholecystolithotomy for elderly patients with acute calculous cholecystitis is safe and feasible,with an advantage of promotional value.
9.Effects of Enema Combined with Massage Therapy on Jaundice in Premature Infants
yin-hua, CHEN ; yan, CUI ; hui-zhu, LI ; qin, ZHOU
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To explore the clinical effects of enema combined with massage therapy on jaundice in premature infants.Methods Seventy-five premature infants with jaundice were randomly divided into 3 groups,enema combined message therapy group(group A),abdominal massage therapy group(group B),and double-side phototherapy group(group C).All 3 groups were received the same formula fee-ding,intravenous nutrition and identical drug treatment.Group A was given enema with mixed kaiselu and normal saline together with 60 times clockwise abdominal massage once a day for 2 weeks.Group B only received abdominal massage twice per day for 2 weeks.Transcutaneous bilirubin(TB) indexes of all the premature infants in the 3 groups were detected and transformed into total TB concentrations every morning,through version of MINOLTA JM-102 transcutaneous bilirubin radiometer made in Japan.When TB index counted more than 196.58 ?mol/L,group A and B were given single-side phototherapy for 24 hours.Neither enema nor abdominal massage was given to group C,and double-side phototherapy was applied when TB indexes were above 196.58 ?mol/L.Daily TB indexes,duration of jaundice and phototherapy,time of meconium exhaustion,defecation times in each day,incidence of constipation and feeding intolerance were recorded.Results Duration of jaunhospitalized and phototherapy were significantly shortened in group A compared with those of the other groups.In 34 premature infants who were hospital for at least 2 weeks,TB indexes in group C were lower than those in group B on the 9th day.On the 12th day and the 14th day,TB indexes in group A and C were lower than those in group B(Pa
10.Toxic Reactions of Memantine in Acute Toxicologic Experiment in Neonatal Rats
ying, GAO ; hui-jin, CHEN ; long-hua, QIAN
Journal of Applied Clinical Pediatrics 2004;0(08):-
Objective To explore the acute toxic reactions of memantine in neonatal rats. Methods Based on Completely Lethal dose(LD_(100)) and median lethal dose (LD_(50))of memantine in SD neonatal rats acquired in a preliminary test of death dose, 60 neonatal rats were randomly divided into normal group which were given water injection intraperitoneally and 5 study groups which were given different doses of memantine intraperitoneally.LD_(50) was calculated with Bliss method and the toxic reactions of memantine were observed in all neonatal rats of 6 groups after administration of memantine. Results LD_(50) of memantine in SD neonatal rats was((74.386?2.811)) mg/kg with 95% confidence at the range of 59.334-93.257 mg/kg.Side effects occurred at 1-4 minutes after administration. Excitatory jitteriness,ataxia,decreased respiratory rate and passivity were usually observed in groups with a lower dosage (52.0 mg/kg,61.2 mg/kg,72.0 mg/kg);some of them also manifested side lying, cyanosis and respiratory failure.While neonatal rats with a higher dosage (85 mg/kg,100 mg/kg)mainly manifested visible symptoms of inhibition, respiratory failure,side (lying) and cyanosis.However,no jitteriness and ataxia were observed in them.The neonatal rats usually died around 1 hour after memantine administration;survival rats usually returned to normal 4-5 hours after administration.Conclusion There is a positive correlation between toxic reactions and the mortality with memantine dosage in neonatal rats.