Objective To observe the clinical efficacy of acupuncture-moxibustion in treating urinary retention after anorectal operation.Method Forty patients with urinary retention after anorectal operation were randomly allocated to a treatment group and a control group, 20 cases each. The treatment group was intervened by acupuncture -moxibustion, and the control group by oral administration of Pyridostigmine bromide tablets. For the two groups, 10 d treatments were taken as a course. Two treatment courses later, the residual urine volume and spontaneous micturition were measured to see the changes, and the clinical efficacies were also compared. Result The total effective rate was 90.0% in the treatment group versus 70.0% in the control group, and the between-group difference was statistically significant (P<0.05). The residual urine volume was significantly changed after the treatment in both groups (P<0.05). After the intervention, the residual urine volume in the treatment group was significantly different from that in the control group (P<0.05). After the treatment, the mean spontaneous micturition time was (16.52±3.18)min in the treatment group, versus (41.37±5.63)min in the control group, and the between-group difference was statistically significant (P<0.05).Conclusion Acupuncture-moxibustion is an effective approach in treating urinary retention after anorectal operation, since it can reduce the residual urine volume and shorten the spontaneous micturition duration.

Objective: To investigate the effect of ionization on the expression of HIF-1 α and VEGF in anoxia condition, in order to search an effective method for improving the radiosensitivity. Methods: HepG2 hepatoma carcinoma cells were divided into four groups: the control group; the hypoxia group; the radiation group; the hypoxia and radiation group. The cell apoptosis was detected by fluorescence microscope. The cell viability was analyzed by MTY method. And we measured the expressions of HIF-1 α mRNA and VEGF mRNA by RT-PCR. Results: Cell apoptosis: no apoptosis was observed in the control group; few apoptosis occurred in the hypoxia group; bulk apoptosis was observed in the radiation group; less apoptosis occurred in the hypoxia and radiation group than that in the hypoxia group. The order of surviving fraction was the control group > the hypoxic group > the hypoxia plus radiation group > the radiation group; The order of the expression of HIF-1 α mRNA: the hypoxia plus radiation group > the hypoxic group > the radiation group, no significant difference was observed between the radiation group and the control group; The order of the expression of VEGF mRNA: the hypoxia plus radiation group > the hypoxic group > the radiation group > the control group. Conclusion: The expression of HIF-1α induced by hypoxia can prevent hepatoma cells from the damage of radiation. The HIF-1 α decreases the radiosensitivity through inducing VEGF expression in the HepG2 cell line.

Myeloid differentiation protein-2(MD-2)can separately and simultaneously bind lipopolysaccharide(LPS)and toll-like receptor 4(TLR4)has been shown to play critical roles in mediated recognition responses to LPS by TLR4 and signal transduction induced by LPS.MD-2 can be bound by LPS,not TLR4.The cells have no responsiveness or weak responsiveness to LPS without MD-2.MD-2 can be secreted into blood plasma,formed soluble MD-2 and remotely regulated cells that contained TLR4 without MD-2.MD-2 has been shown to play important roles in endotoxin signal transduction.MD-2 is a small molecular,short nucleic acid fragment,easily regulated should become a new potential anti-inflammatory target.

S100A4, a member of S100 superfamily of Ca~(2+)-binding proteins, is a polypeptide containing 101 amino acids. S100A4, which is overexpressed in most tumor cells, plays pivotal roles in growth, invasion and migration of tumor by regulating cell cycle progression, differentiation, apoptosis, invasion and metastasis of cells. This article reviews S100A4′s structure, function and potential application as the new target in the therapy of tumors.

Objective To understand the mechanism of cerebral energy failure after hypoxia ischemia at the molecular level and to establish the protocol for the safe and effective treatment of hypoxic-ischemic encephalopathy(HIE).Methods One hundred neonatal rats were divided into normal control group and hypoxic-ischemic(HI) group. SD rats of both groups were decapitated at the time of 2 h,24 h,48 h,72 h and 7 d after HI.These tissues of cerebrum,cortex and hippocampus were taken out to explore the influence of HI on the expression of GLUT1 and GLUT3 genes with the method of RT-PCR.Results There was an enhancement in the expression of GLUT1 and GLUT3 genes with the increasing of day age. The expression was more intense in hippocampus than that in cortex. However, HI could significantly enhance the expression of GLUT genes. The expression was higher in cortex than that in hippocampus. The expression of two genes reached the peak at 24 h after HI, but was significantly lower than that in control group at 7 d after HI.Conclusion The increased expression of GLUT genes can maintain the energy supplement for the brain and delay a cascade reaction of cerebral energy failure.

Adult ; Humans ; Lipids ; blood ; Male ; Occupational Exposure ; Vanadium ; toxicity

Child ; Humans ; Hyperplasia ; Male ; Panniculitis ; diagnosis ; Skin Diseases ; diagnosis ; T-Lymphocytes ; pathology

Animals ; Animals, Newborn ; Drugs, Chinese Herbal ; therapeutic use ; Excitatory Amino Acid Antagonists ; therapeutic use ; Hypoxia-Ischemia, Brain ; metabolism ; prevention & control ; Memantine ; therapeutic use ; Platelet Activating Factor ; analysis ; Rats ; Rats, Sprague-Dawley

Objective Inquire into the relationship between diabetic peripheral neuropathy(DPN)pathogenic factor and Antigangliosides antibody(Anti-GS-Ab)in type 2 diabetes mellitus. Methods It was examined by enzyme-linked immunosorbent assays(ELISA)to the levels of serum Anti-gangliosides(Anti-GS)in 2 DM and DPN as well as healthy.Results The positive rate of Anti-GS-IgM,IgG in DPN group were 46.7% and 20.0%.repectivily it was obviously higher than normal group and 2 DM group.Conclusion The relationship between the DPN and Anti-GS-Ab is a close.It show that Anti-GS-Ab play an important role in DPN pathological process.

Objective To explore the effect of DR on the expression of inhibitory neurotransmitter ,γ-aminobutyric acid ( GABA) and brain derived neurotrophic factor ( BDNF) in the primary visual cortex of young cats .Methods Totally 6 cats were wsed in this study . Nissl staining was used for cortical layer identification and cell counting . Immunohistochemical techniques were utilized to label GABA-and BDNF-positive neurons .Sections were observed under an Olympus light microscope and photographed with a digital camera .The cell density and absorbance of immunoreactivity were measured with Image-Pro Express 6.0 softwares .Results Our results showed that the mean density of Nissl-stained neurons in the primary visual cortex ( V1) of the DR group showed no significant difference from that of the control group . However , the mean density of GABA-immunoreactive neurons in each cortical layer of V 1 in DR cats was significantly higher than that in control cats .The mean immunoreactive intensity of GABA-positive neurons , as indicated by the average absorbance , increased significantly in DR cats relative to control ones . Accompanied with the elevation of GABA expression, DR enhanced BDNF expression in V1, as indicated by an increased mean density of BDNF-positive neurons and BDNF-immunoreactive average absorbance in DR cats relative to controls .Conclusion These results indicate that dietary restriction leads to a concurrent expression upregulation of GABA and BDNF , which may likely compensate for brain functional degradation during senescence and thus delay aging process .