2.Progress on Biotechnological Production of Butyric Acid
Ming-Rong DENG ; Jun GUO ; Hong-Hui ZHU ;
China Biotechnology 2006;0(03):-
Butyric acid can be used to produce cellulose butyrate fiber and ester derivatives,and to be applied in foodstuffs and perfume industries.Recent researches have found that butyric acid is a preferred carbon source for colonic epithelial cells,and can inhibit histone deacetylase,showing great anticancer potentials.With more and more functions of butyric acid being found and applied in bio-related fields,and with consumer's growing preference to bioproducts,biotechnological production of butyric acid will receive more competence than petroleum-based chemical synthesis.Low product concentration and poor selectivity are presently the main restricting factors.Workers have made considerable progress on more cheep carbon sources,optimization of fermentation process,simplifying downstream processing,and genetic engineering of producing strains.Any achievement on these aspects in the future can contribute to put fermentation of butyric acid into industry.
3.Application of low dose radiation therapy in the treatment of COVID-19 pneumonia
Hui LUO ; Hong GE ; Wenna NIE ; Hui LIU ; Yamei HU ; Wenjuan XU ; Guodong DENG
Chinese Journal of Radiological Medicine and Protection 2021;41(2):151-154
With the global pandemic of COVID-19, cytokine storms in critical patients with pneumonia is really a problem and need to be solved immediately.Low dose radiation therapy (LDRT) has been temporarily used to treat pneumonia.In the past decades, researchers were dedicated to clarify the biological mechanism of LDRT.LDRT plays a unique role in the suppression of inflammation, preliminary outcomes have been acquired in critical patients with COVID-19 pneumonia, and radiotherapy community is paying attention to this treatment strategy.This review summarizes the application of LDRT in pneumonia, its biological mechanism, the result of LDRT in COVID-19 pneumonia, the existing problems and prospective in clinic.
4.Advance of Ser/Thr Kinase Pololike Kinase 1 (Plk1) Research
Fu-Yin XIONG ; Hui-Yuan LIU ; Hong-Xing CHEN ; Ji-Xian DENG ;
China Biotechnology 2006;0(05):-
Pololike kinase 1(Plk1)contain an Nterminal Ser/Thr kinase catalytic domain and a Cterminal region that contains two poloboxes.As a key regulator of multiple steps during cell cycle across eukaryotic species,many proteins interact with Plk1.Plk1 is highly expressed in malignant cells and serves as a negative prognostic marker in specific human cancer types.Plk1 is a potential target for cancer therapy.Some novel smallmolecule inhibitors of pololike kinase 1 provide novel opportunities for cancerdrug discovery,such as BI 2536,ON01910.
5.Expression of Lck in renal tubule epithelial cells of lupus nephritis
Ying-Hui DENG ; Qing-Gang LI ; Chong-Yu REN ; Xiu-Hong XU ;
Chinese Journal of Rheumatology 2003;0(07):-
Objective To investigate the Lymphocyte-specific protein-tyrosine kinase(Lck)gene ex- pression in the renal tubule epithelial cells(TECs)of lupus nephritis,and the effect of interlenkin-2(IL-2) stimulation on its expression.Methods Proximal TECs derived from 6 weeks old spontaneous systemic lupus erythematosus(SLE)BXSB mice were exposed to IL-2(100 U/ml),the expression of Lck mRNA and protein was examined by reverse transcription-polymerase chain reaction(RT-PCR)and immunoblotting respectively. The difference of Lck gene expression before and after IL-2 stimulation was investigated.The expression of Lck protein in TECs of renal tissues of BXSB mice and human with lupus nephritis was observed through im- munohistochemistry.Results The expression of Lck mRNA and protein was very low in cultured TECs of 6 weeks old BXSB mice,but increased sharply after IL-2 stimulation(P
6.Study on totai flavonoids of Epimedium assisted with soybean polysaccharide spray-drying powder.
Hong-mei YAN ; Xiao-bin JIA ; Zhen-hai ZHANG ; E SUN ; Jia-hui DENG
China Journal of Chinese Materia Medica 2015;40(15):2994-2998
In order to evaluate the characteristics of the spray drying of total flavonoids of Epimedium extracts assisted with soybean polysaccharide, a certain percentage of soybean polysaccharide or polyvidone were added to the total flavonoids of Epimedium extract to conduct the spray drying. The effect of soybean polysaccharides against the wall sticking effect of the spray drying was detected, as well as the powder property of total flavonoids of Epimedium spray drying powder and the dissolution in vitro behavior of the effective component. Compared with the total flavonoids of Epimedium spray drying powder, soybean polysaccharide revealed a significant anti-wall sticking effect. The spray drying power which had no notable change in the grain size made a increase in the fluidity, improvement in the moisture absorption and remarkable rise in the dissolution in vitro behavior. It was worth further studying the application of soybean polysaccharide in spray drying power of traditional Chinese medicine.
Epimedium
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chemistry
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Flavonoids
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analysis
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Particle Size
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Polysaccharides
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chemistry
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Powders
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Soybeans
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chemistry
7.Relationship of haplotypes of FgBbeta-1420G/A -993C/T, and BsmAIG/C with functional expression and cerebral infarction.
Nan-nan ZHANG ; Xiao-dong YUAN ; Jian-hui XU ; Hong-liang DENG ; Shu-juan WANG
Chinese Journal of Applied Physiology 2012;28(3):218-220
Aged
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Case-Control Studies
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Cerebral Infarction
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blood
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genetics
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Female
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Fibrinogen
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genetics
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metabolism
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Haplotypes
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Humans
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Male
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Middle Aged
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Polymorphism, Genetic
8.Protective Effects of Tempol on Ultraviolet-AI Irradiated Human Dermal Fibroblasts
Shuxian YAN ; Yue HU ; Hui DENG ; Xinyu HONG ; Yu XU ; Kanghuang LIAO
Chinese Journal of Dermatology 2003;0(07):-
Objective To determine the effects of Tempol, one of the nitroxides, in the presence of ultraviolet-AI (UVA1, 340 nm -400 nm) on superoxide enzyme (SOD) activity, lipid peroxidation, and expression of collagen Ⅰ, collagen Ⅲ and matrix metalloproteinases (MMP)-1, MMP-3 in human dermal fi broblasts in vitro. Methods Fibroblasts were irradiated by a single exposure to UVA1 and at the same time incubated with or without Tempol, and detected 24 h later. SOD activity and lipid peroxidation, as shown by accumulation of malondialdehyde (MDA), were detected by biochemical assay. Expression of collagen Ⅰ, collagen Ⅲ (protein levels) and MMP-1, MMP-3 (mRNA level) was examined by ELISA and semi-quantitative RT-PCR, respectively. Results A dose of 15 J/cm2 UVA1 significantly inhibited SOD activity and collagen Ⅰ , collagen Ⅲ protein levels, increased MDA level and stimulated MMP-1, MMP-3 mRNA expression (P
9.The physiological effects of resveratrol and its potential application in high altitude medicine.
Hui-li ZHU ; Hong-jing NIE ; Pei-bing LI ; Bing-nan DENG ; Rui-feng DUAN ; Hong JIN ; Zhao-li CHEN
Chinese Journal of Applied Physiology 2015;31(6):498-503
Resveratrol, as a natural polyphenolic compound, has a wide range of beneficial effects, which includes anti-tumor, cardiovascular protection, anti-oxidant and estrogen-like effects, and so on. Its various physiological properties are closely related to the therapeutic principle for prevention and treatment of high altitude hypoxia injury. Resveratrol may play an important role in relieving or curing high altitude diseases, especially high altitude polycythemia(HAPC). However, the literature about study and application of resveratrol in plateau medicine field is rarely reported up to now. In this review, we summarized the physiological effects of resveratrol, discussed the possible main principle of resveratrol for HAPC therapy, and looked forward to resveratrol's perspective or potential application in high altitude medicine.
Altitude
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Humans
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Hypoxia
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drug therapy
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Polycythemia
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drug therapy
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Stilbenes
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pharmacology
10.The value of multi-slice CT and MRI for early complications of inguinal hernia repair
Yong ZHANG ; Yumei ZHOU ; Shujun CHEN ; Hong LI ; Zhouxuan WANG ; Qingtao HUI ; Hongyi DENG ; Banggao NI ; Bing MING
Journal of Practical Radiology 2015;(4):580-583
Objective To evaluate the value of MSCT and MRI in diagnosis of early postoperative complications of inguinal hernia repair (IHR).Methods Imaging and clinical data in 1 6 patients with early complications of IHR were analyzed retrospectively.Re-sults Among the complications,postoperative infection in 2 was found including incision infection in 1 and groin cellulitis in other 1. CT showed swelling abdominal wall and heterogeneous enhancement for incision infection,and inguinal mass,deep inguinal ring thickening,edema of residual sac with fluid and air collections for groin cellulitis.Seroma was found in 8,and CT and MRI demon-strated residual sac effusion,spermatic cord thickening and spermatic vascular tortuosity.Residual sac edema was found in 4,and CT and MRI showed thickening spermatic cord without effusion in residual sac or scrotum.Effusions between patch and anterior abdom-inal wall were detected by MRI in 2,one of which was accompanied by patch shrinking.Conclusion With specific clinical background for early complications of IHR,MSCT and MRI provide more anatomical information of inguinal region,which may contribute to di-agnosis and treatment of early postoperative complications.