OBJECTIVETo investigate the effect of Sanleng pellet on angiogenesis of endometriosis in SD rats.

METHODThe rat model of endometriosis were treated with Sanleng pellet. Observe microvessels density by immunohistochemical test as well as the expression of vascular endothelial growth factor and tumor necrosis factor-alpha in ectopic endometriotic tissue by immunohistochemical test and RT-PCR.

RESULTSanleng pellet can reduce MVD, and inhibit the level of protein and mRNA of vascular endothelial growth factor and TNF-alpha in ectopic endometriotic tissue.

CONCLUSIONSanleng pellet can reduce the degree of angiogenesis in the endometriosis rats, and its mechanism might be associated with inhibiting the expression of vascular endothelial growth factor and TNF-alpha in the heterotopic tissue.

Animals ; Drugs, Chinese Herbal ; therapeutic use ; Endometriosis ; drug therapy ; metabolism ; Female ; Immunohistochemistry ; Neovascularization, Pathologic ; drug therapy ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Necrosis Factor-alpha ; genetics ; metabolism ; Vascular Endothelial Growth Factor A ; genetics ; metabolism