Objective To evaluate the diagnostic value of serum angiopoietin-2 (Ang-2) level for gastric cancer.Methods Serum Ang-2 level waa measured by enzyme-linked immunosorbent assays (ELISA) in samples from 108 patients with gastric cancer,50 patients with chronic gastritis and 50 normal controls.Results The serum Ang-2 level in patients with gastric cancer [(1539.0 ± 449.3) ng/L] was significantly higher than that in patients with gastritis [(1044.6 ± 246.1) ng/L,P ＜ 0.01] and normal control [(1075.6 ± 228.2) ng/L,P ＜ 0.01)].There was no significant difference of serum Ang-2 level between patients with gastritis and normal control.Gastric cancer patients with lymph node metastasis had significantly higher serum Ang-2 level than those without metastasis (P ＜0.01).The area under ROC curve of serum Ang-2 level for diagnosis of gastric cancer was 0.819.Conclusion The serum Ang-2 level will be a useful indicator for diagnosis of gastric cancer.

Objective: To construct the eukaryotic expression recombinant plasmid of cadF gene of Campylobacter jejuni Pen: 19 and to identify its express in COS-7 cells.Methods: Total DNA of Campylobacter jejuni Pen:2?Pen:8?Pen:19 and Pen: 21 were used as templates respectively and cadF gene DNA with KpnI and EcoRI sites were amplified by PCR reactions using same primer.Because of its closest association with Guillain-Barr? syndrome,the PCR product of Pen:19 was selected as target gene and inserted into the eukaryotic expression vector pcDNA3.1(+) and the recombinant plasmid pcDNA3.1(+)-cadF was constructed.The recombinant plasmid was identified by restriction enzyme digestion and PCR reaction and was confirmed by sequencing.The recombinant plasmid was then transfected into mammalian cell COS-7 for mRNA expression and the expression were investigated by RT-PCR reaction.Results: It was confirmed with restriction enzyme digestion and PCR reaction and sequencing that cadF gene was cloned into pcDNA3.1(+) successfully.The objective gene was amplified from COS-7 cell transfected with the recombinant plasmid pcDNA3.1(+)-cadF by RT-PCR.Conclusion: The eukaryotic expression recombinant plasmid pcDNA3.1(+)-cadF is constructed and expressed in COS-7 cell successfully.The results obtained lay the foundation for research on development of Campylobacter jejuni DNA vaccine.

The study on human embryonic stem cell regeneration is one of the current hot issues of life science.Dialectically analyzing its benefits and harms and the scientific application of the therapy of human diseases.It will surely initiate a new model of clinic treatment.

BACKGROUND: Severe or prolonged stress is harmful to health and even induces many mental and physical disorders and then impairs cognitire functions of the organism.OBJECTIVE: To investigate the effects of stress on the changes in amino acid and chronic stress on long-term potentiation (LTP) of hippocampus of rats.DESIGN: Completely randomized controlled experiment.SETTING: Mental Health Center of Shantou University.MATERIALS: The experiment was completed in the Medical College of Shantou University in December 2000. Totally 16 adult male SD rats were divided randomly into control group and stress group with 8 in each group.METHODS: Mice in stress group were swum compulsively for 4 weeds.Chronic stress model was established, and isolated hippocampal slice(500 μm) combined with electrophysiological technique was used to observe the changes of LTP in hippocampal CA1 field. High-frequency stimulation (HFS) was applied to Schaffer collaterals in hippocampus CA3, then the changes of PS amplitude and fEPSPs slope in CA1 pyramidal neurons were observed. High performance liquid chromatography (HPLC) coupled with UV detection was used for the quantificative analysis of hippocampal amino-acid neurotransmitters.MAIN OUTCOME MEASURES: ① Population spike (PS) amplitude and field excitatory postsynaptic potentials (fEPSPs) slope were used to indicate the changes of LTP. ② Changes of amino acid of hippocampus.RESULTS: One rat in stress group died because of drowning in the second week of experiment was supplied, and others entered the final analysis. ① The increases of PS amplitude and fEPSPs slope after HFS in control group were significantly higher than those in stress group (P＜ 0.05). ② The levels of aspartate and glutamate in control group were significantly lower than those in stress group [(2.425±0.211) μmol/g,(6.016±0.677) μmol/g. P＜ 0.01; (4.746±0.609) μmol/g. (8.094±1.035) μmol/g.P ＜ 0.01]; but there was no significant difference of γ-aminobutyric acid between control and stress groups [(4.229±0.449) μmol/g, (4.249± 0.463) μmol/g, P ＞ 0.05].CONCLUSION: It is suggested that chronic stress can inhibit the development of LTP in hippocampal CA1 field and increase the levels of hippocampal aspartate and glutamate, but does not affect the level of γ-aminobutyric acid. The accumulation of hippocampal excitatory amino acids induced by chronic stress may be the neurobiochemical basis of impairments in learning and memory abilities.

Objective To study the outcome of pregnancy with lupus nephritis. Methods The clinical data of 9 pregnancies with lupus nephritis were retrospectively malyzed. Results Hypertension,proteinuria,injured renal function and abnormal serological marker were complicated with nine patients with lupus nephritis in pregnancy. The outcome of pregnancy was as following:abortion 2 cases,stillbirth 1 case,premature delivery 4 cases,term delivery 2 cases. All neonate survived without malformation. There was no postpartum hemorrhage or death. Patients with active SLE had very high fetal mortality( 100% ). And SLE got worsen in 50% of them. However,fetal mortality rate in pa-tients with controlled or quiescent SLE was 11.1% ,which was much lower than it in other patients(P<0.05). Con-clusion Lupus nephritis should be controlled before pregnancy. Pregnancy outcome could be improved by careful multidisciplinary monitoring and proper usage of adrenal cortical hormone.

At present,in the opinions of the domestic and foreign scholars,the only hope of long-term survival even cure of hilar cholangiocarcinoma was obtained by radical resection(ie R0 resection).The application of preoperative assessment with appropriate and comprehensive strategies can judge the respectability and Intraoperative safety accurately to the tumor,thereby it can improve the radical resection rate and safety of patients with hilar cholangiocarcinoma.In this paper,preoperative assessment strategies are reviewed.

A totel of 120 patients with CT or MRI proved as acute cerebral infarction group of anterior circulation and 90 non-infarction patients as the control group were studied. The diameter,inter median thickness( IMT) ,peak systolic velocity ( PSV ) , end diastolic velocity ( EDV ) , early shrinkage acceleration time ( AT ) , resistance index ( RI) ,and pulsatility index( PI) of carotid artery,and whether there was arteriosclerotic plaque or stenosis,and the properties of plaque were examined with color Doppler ultrasound. In acute cerebral infarction group of anterior cir-culation,the IMT was thicker that in the control group(P<0. 01). The incidence of the film thickness,moderate-se-vere stenosis and instable carotid artery plaque in infaiction group of anterior circulation were obviousely higher than those in the control group(P<0. 01). Arterial complete or incomplete occlusion was observed in severe cases. The blood flow parameters showed that the PSV and EDV of common carotid and internal carotid in acute cerebral in-farction group of anterior circulation were lowerer than those in the control group. RI and PI of internal carotid in a-cute cerebral infarction group of anterior circulation were higher than those in the control group(P<0. 05),while AT was lower than those in the control group.

Objective To explore the therapeutic mechanism of Xiao Chaihu Decoction for endometriosis in rats. Methods The rat models of endometriosis were treated with Xiao Chaihu Decoction, Danazol, and Xiao Chaihu Decoction plus Danazol for 4 weeks,respectively. And then cyclooxygenase-2(COX-2) expression in endometrium and endometriotic tissue was observed by immunohistochemistry method. Results The expression of COX-2 in the endometriotic tissue was lower than that in endometrium in Xiao Chaihu Decoction group and Xiao Chaihu Decoction plus Danazol group, while the results in Danazol group and control group were opposed to those in the above two groups. Conclusion The mechanism of Xiao Chaihu Decoction for rats endometriosis may be related to the decrease of COX-2 expression in ectopic Endometrium, thus reducing the formation of prostaglandins.

Objective To evaluate the effect of color Doppler ultrasound monitoring in artificial arteria-venous fistula in patients with uraemia.Methods 25 cases were examined with upper limbs arteriovenous fistulas with uremia and analysed by CDFI and 2D.The fistula and blood vessel was observe and hemodynamics changes were examined.Results Among 25 patients,18 cases showed good filling state of the vessel,3 cases with anastomoses stenosis,2cases with thrombus and 2cases with venous dilatations.Conclusion CDFI is an effective supplementary detective methods in treating hemodialysis patients with uremia.

Objective To explore the influence of ICAM-1 and LFA-1 on the breast cancer metastasis and provide scientific base for studying the molecular mechanism of breast cancer metastasis by observing the expression of ICAM-1(intercellular adhension molecule1) and LFA-1(Lymphocyte function related antigen 1) in different time different metastasis stage of breast cancer. Methods We observed the expression of ICAM-1 and LFA-1 in breast cancer tissue as well as the expression of ICAM-1、LFA-1 and VEGFR-3 in Lymphatic vessel endothelial cell of nearby the breast cancer tissues from the patients of the operation in the defferent stages with the methods of HE staining and immunohistochemistry staining. Results The positive rate of ICAM-1 and LFA-1 expression in cancer cell and cancer nest of breast cancer was increased gradually along with the increase of clinical stages and the occurrence of lymph node metastasis. And the positive rate of ICAM-1、LFA-1 and VEGFR-3 expression in lymphatic vessel endothelial cell of nearby the breast cancer tissues was increased gradually too along with the increase of clinical stages and the occurrence of lymph node metastasis,which has the positive correlation,and it has the positive correlation that the ICAM-1 and LFA-1 expressed in Lymphatic vessel endothelial cell of nearby the breast cancer tissues(P0.05).Conclusion The ICAM-1 and LFA-1 were related with breast cancer lymph metastasis,and the ICAM-1 and LFA-1 promoted breast cancer lymph metastasis through synergism each other.The increase or decrease of ICAM-1 and LFA-1 expression in the breast cancer tissue and lymphatic vessel endothelial cell of nearby the breast cancer tissues could as the data target to display certain instruction function for prevention and treatment of breast cancer lymph metastasis.The Lymphatic vessel quantity of nearby the breast cancer tissues was increased along with tumor progress, which may be related with breast cancer lymph metastasis.VEGFR-3 has the high specificity in the lymphatic vessel endothelial cell expression,and may act as the lymphatic vessel as the lymphatic vessel endothelial cell specific marker.